Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_009_C03
(800 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P13230|GRP33_ARTSA Glycine-rich protein GRP33 126 8e-29
sp|Q60749|SAM68_MOUSE KH domain containing, RNA binding, si... 117 5e-26
sp|Q07666|SAM68_HUMAN KH domain containing, RNA binding, si... 117 5e-26
sp|Q91V33|SAM68_RAT KH domain containing, RNA binding, sign... 117 5e-26
sp|Q8UUW7|SAM68_CHICK KH domain containing, RNA binding, si... 117 5e-26
sp|O01367|HOW_DROME Held out wings protein (KH-domain prote... 83 1e-15
sp|Q17339|GLD1_CAEEL Female germline-specific tumor suppres... 77 5e-14
sp|Q15637|SF01_HUMAN Splicing factor 1 (Zinc finger protein... 51 3e-06
sp|Q64213|SF01_MOUSE Splicing factor 1 (Zinc finger protein... 50 5e-06
sp|P42083|YKCA_CAEEL Hypothetical protein B0280.11 in chrom... 48 4e-05
>sp|P13230|GRP33_ARTSA Glycine-rich protein GRP33
Length = 308
Score = 126 bits (316), Expect = 8e-29
Identities = 62/125 (49%), Positives = 88/125 (70%), Gaps = 7/125 (5%)
Frame = +3
Query: 303 IPVEAYPNFNFVGKLLGPAGKTLKTVQEQTNTKMSILGAGSLRNPEKEQELLNSGDPKYN 482
+PV+ +P +NF+GKLLGP G T+K +Q++T TK+SILG GS+R+ KE+EL NSGD KY
Sbjct: 80 LPVDQFPKYNFLGKLLGPGGSTMKQLQDETMTKISILGRGSMRDRNKEEELRNSGDVKYA 139
Query: 483 HLRDRLHLQIDSIGNPSEAYYNISYALAEVKKVMIP--DPN-----EQAPGQMPMVTPLM 641
HL ++LH++I SI +P+EA+ ++YAL E+KK + P DPN G M + +M
Sbjct: 140 HLNEQLHIEIISIASPAEAHARMAYALTEIKKYITPEEDPNYMMMAGHGAGPMMGMGGMM 199
Query: 642 GGPNP 656
GGP P
Sbjct: 200 GGPGP 204
>sp|Q60749|SAM68_MOUSE KH domain containing, RNA binding, signal transduction associated
protein 1 (p21 Ras GTPase-activating protein-associated
p62) (GAP-associated tyrosine phosphoprotein p62)
(Src-associated in mitosis 68 kDa protein) (Sam68) (p68)
Length = 443
Score = 117 bits (292), Expect = 5e-26
Identities = 59/122 (48%), Positives = 81/122 (66%), Gaps = 1/122 (0%)
Frame = +3
Query: 303 IPVEAYPNFNFVGKLLGPAGKTLKTVQEQTNTKMSILGAGSLRNPEKEQELLNSGDPKYN 482
IPV+ YP FNFVGK+LGP G T+K +QE+T K+S+LG GS+R+ KE+EL GDPKY
Sbjct: 162 IPVKQYPKFNFVGKILGPQGNTIKRLQEETGAKISVLGKGSMRDKAKEEELRKGGDPKYA 221
Query: 483 HLRDRLHLQIDSIGNPSEAYYNISYALAEVKKVMIPD-PNEQAPGQMPMVTPLMGGPNPG 659
HL LH+ I+ G P EAY +++A+ EVKK ++PD ++ Q ++ L G P P
Sbjct: 222 HLNMDLHVFIEVFGPPCEAYALMAHAMEEVKKFLVPDMMDDICQEQFLELSYLNGVPEPS 281
Query: 660 FG 665
G
Sbjct: 282 RG 283
>sp|Q07666|SAM68_HUMAN KH domain containing, RNA binding, signal transduction associated
protein 1 (p21 Ras GTPase-activating protein-associated
p62) (GAP-associated tyrosine phosphoprotein p62)
(Src-associated in mitosis 68 kDa protein) (Sam68) (p68)
Length = 443
Score = 117 bits (292), Expect = 5e-26
Identities = 59/122 (48%), Positives = 81/122 (66%), Gaps = 1/122 (0%)
Frame = +3
Query: 303 IPVEAYPNFNFVGKLLGPAGKTLKTVQEQTNTKMSILGAGSLRNPEKEQELLNSGDPKYN 482
IPV+ YP FNFVGK+LGP G T+K +QE+T K+S+LG GS+R+ KE+EL GDPKY
Sbjct: 162 IPVKQYPKFNFVGKILGPQGNTIKRLQEETGAKISVLGKGSMRDKAKEEELRKGGDPKYA 221
Query: 483 HLRDRLHLQIDSIGNPSEAYYNISYALAEVKKVMIPD-PNEQAPGQMPMVTPLMGGPNPG 659
HL LH+ I+ G P EAY +++A+ EVKK ++PD ++ Q ++ L G P P
Sbjct: 222 HLNMDLHVFIEVFGPPCEAYALMAHAMEEVKKFLVPDMMDDICQEQFLELSYLNGVPEPS 281
Query: 660 FG 665
G
Sbjct: 282 RG 283
>sp|Q91V33|SAM68_RAT KH domain containing, RNA binding, signal transduction associated
protein 1 (p21 Ras GTPase-activating protein-associated
p62) (GAP-associated tyrosine phosphoprotein p62)
(Src-associated in mitosis 68 kDa protein) (Sam68) (p68)
Length = 443
Score = 117 bits (292), Expect = 5e-26
Identities = 59/122 (48%), Positives = 81/122 (66%), Gaps = 1/122 (0%)
Frame = +3
Query: 303 IPVEAYPNFNFVGKLLGPAGKTLKTVQEQTNTKMSILGAGSLRNPEKEQELLNSGDPKYN 482
IPV+ YP FNFVGK+LGP G T+K +QE+T K+S+LG GS+R+ KE+EL GDPKY
Sbjct: 162 IPVKQYPKFNFVGKILGPQGNTIKRLQEETGAKISVLGKGSMRDKAKEEELRKGGDPKYA 221
Query: 483 HLRDRLHLQIDSIGNPSEAYYNISYALAEVKKVMIPD-PNEQAPGQMPMVTPLMGGPNPG 659
HL LH+ I+ G P EAY +++A+ EVKK ++PD ++ Q ++ L G P P
Sbjct: 222 HLNMDLHVFIEVFGPPCEAYALMAHAMEEVKKFLVPDMMDDICQEQFLELSYLNGVPEPS 281
Query: 660 FG 665
G
Sbjct: 282 RG 283
>sp|Q8UUW7|SAM68_CHICK KH domain containing, RNA binding, signal transduction associated
protein 1 (Src-associated in mitosis 68 kDa protein)
(Sam68)
Length = 433
Score = 117 bits (292), Expect = 5e-26
Identities = 62/137 (45%), Positives = 83/137 (60%), Gaps = 10/137 (7%)
Frame = +3
Query: 303 IPVEAYPNFNFVGKLLGPAGKTLKTVQEQTNTKMSILGAGSLRNPEKEQELLNSGDPKYN 482
IPV+ YP FNFVGK+LGP G T+K +QE+T K+S+LG GS+R+ KE+EL GDPKY
Sbjct: 145 IPVKQYPKFNFVGKILGPQGNTIKRLQEETGAKISVLGKGSMRDKAKEEELRKGGDPKYA 204
Query: 483 HLRDRLHLQIDSIGNPSEAYYNISYALAEVKKVMIPDPNEQAPGQ----------MPMVT 632
HL LH+ I+ G P EAY +++A+ EVKK ++PD + + +P T
Sbjct: 205 HLNMDLHVFIEVFGPPCEAYALMAHAMEEVKKFLVPDMMDDICQEQFLELSYLNGVPEPT 264
Query: 633 PLMGGPNPGFGAMRTQP 683
GGP G GA P
Sbjct: 265 RGRGGPVRGRGAAPPPP 281
>sp|O01367|HOW_DROME Held out wings protein (KH-domain protein KH93F) (Putative
RNA-binding protein) (Muscle-specific protein) (Wings
held out protein) (Struthio protein) (Quaking-related
93F)
Length = 405
Score = 82.8 bits (203), Expect = 1e-15
Identities = 41/105 (39%), Positives = 66/105 (62%)
Frame = +3
Query: 303 IPVEAYPNFNFVGKLLGPAGKTLKTVQEQTNTKMSILGAGSLRNPEKEQELLNSGDPKYN 482
+PV +P+FNFVG++LGP G T K ++++T K+ + G GS+R+ +KE N G P +
Sbjct: 143 VPVREHPDFNFVGRILGPRGMTAKQLEQETGCKIMVRGKGSMRDKKKED--ANRGKPNWE 200
Query: 483 HLRDRLHLQIDSIGNPSEAYYNISYALAEVKKVMIPDPNEQAPGQ 617
HL D LH+ I + A ++ A+AEV+K+++P QA G+
Sbjct: 201 HLSDDLHVLITVEDTENRATVKLAQAVAEVQKLLVP----QAEGE 241
>sp|Q17339|GLD1_CAEEL Female germline-specific tumor suppressor gld-1 (Defective in germ
line development protein 1)
Length = 463
Score = 77.0 bits (188), Expect = 5e-14
Identities = 35/98 (35%), Positives = 58/98 (59%)
Frame = +3
Query: 303 IPVEAYPNFNFVGKLLGPAGKTLKTVQEQTNTKMSILGAGSLRNPEKEQELLNSGDPKYN 482
+P YP++NFVG++LGP G T K +++ T K+ + G GS+R +K +E + G +
Sbjct: 211 VPKNEYPDYNFVGRILGPRGMTAKQLEQDTGCKIMVRGKGSMR--DKSKESAHRGKANWE 268
Query: 483 HLRDRLHLQIDSIGNPSEAYYNISYALAEVKKVMIPDP 596
HL D LH+ + + + + AL +VKK++IP P
Sbjct: 269 HLEDDLHVLVQCEDTENRVHIKLQAALEQVKKLLIPAP 306
>sp|Q15637|SF01_HUMAN Splicing factor 1 (Zinc finger protein 162) (Transcription factor
ZFM1) (Zinc finger gene in MEN1 locus) (Mammalian branch
point binding protein mBBP) (BBP)
Length = 639
Score = 51.2 bits (121), Expect = 3e-06
Identities = 33/107 (30%), Positives = 57/107 (53%), Gaps = 6/107 (5%)
Frame = +3
Query: 303 IPVEAYPNFNFVGKLLGPAGKTLKTVQEQTNTKMSILGAGSLRNPE---KEQELLNSGDP 473
IP + YP NFVG L+GP G TLK ++++ N K+ I G GS++ + K+ ++L D
Sbjct: 142 IPQDEYPEINFVGLLIGPRGNTLKNIEKECNAKIMIRGKGSVKEGKVGRKDGQMLPGED- 200
Query: 474 KYNHLRDRLHLQIDSIGNPSEAYYNISYALAEVKKVM---IPDPNEQ 605
+ LH + + N E N+ A+ +++ ++ I P +Q
Sbjct: 201 ------EPLHALVTA--NTME---NVKKAVEQIRNILKQGIETPEDQ 236
>sp|Q64213|SF01_MOUSE Splicing factor 1 (Zinc finger protein 162) (Transcription factor
ZFM1) (mZFM) (Zinc finger gene in MEN1 locus) (Mammalian
branch point binding protein mBBP) (BBP) (CW17)
Length = 653
Score = 50.4 bits (119), Expect = 5e-06
Identities = 33/107 (30%), Positives = 56/107 (52%), Gaps = 6/107 (5%)
Frame = +3
Query: 303 IPVEAYPNFNFVGKLLGPAGKTLKTVQEQTNTKMSILGAGSLRNPE---KEQELLNSGDP 473
IP + YP NFVG L+GP G TLK ++++ N K+ I G GS+ + K+ ++L D
Sbjct: 142 IPQDEYPEINFVGLLIGPRGNTLKNIEKECNAKIMIRGKGSVEEGKVGRKDGQMLPGED- 200
Query: 474 KYNHLRDRLHLQIDSIGNPSEAYYNISYALAEVKKVM---IPDPNEQ 605
+ LH + + N E N+ A+ +++ ++ I P +Q
Sbjct: 201 ------EPLHALVTA--NTME---NVKKAVEQIRNILKQGIETPEDQ 236
>sp|P42083|YKCA_CAEEL Hypothetical protein B0280.11 in chromosome III
Length = 634
Score = 47.8 bits (112), Expect = 4e-05
Identities = 28/93 (30%), Positives = 49/93 (52%)
Frame = +3
Query: 306 PVEAYPNFNFVGKLLGPAGKTLKTVQEQTNTKMSILGAGSLRNPEKEQELLNSGDPKYNH 485
P E N N VG+L+GP G T++ +++ K+ I G G ++ KE+ L + H
Sbjct: 520 PPETANNTNPVGRLIGPRGMTIRQLEKDLGCKLFIRGKGCTKDDAKEERLRER--VGWEH 577
Query: 486 LRDRLHLQIDSIGNPSEAYYNISYALAEVKKVM 584
L++ +H+ I + EA S L+ +KK++
Sbjct: 578 LKEPIHVMISVRSDSEEA---ASEKLSSIKKML 607
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 75,454,072
Number of Sequences: 369166
Number of extensions: 1378531
Number of successful extensions: 3561
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3432
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3548
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 7570361805
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)