Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_009_A19
(599 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q35905|RMAR_SACDO Mitochondrial ribosomal protein VAR1 33 0.72
sp|P46213|SYI_THEMA Isoleucyl-tRNA synthetase (Isoleucine--... 32 1.6
sp|Q03208|YML9_YEAST Hypothetical 40.1 kDa protein in NDI1-... 30 3.6
sp|P02381|RMAR_YEAST Mitochondrial ribosomal protein VAR1 30 3.6
sp|P51842|GUC2F_RAT Retinal guanylyl cyclase 2 precursor (G... 30 6.1
sp|O14265|VATH_SCHPO Vacuolar ATP synthase subunit H (V-ATP... 29 7.9
sp|P10845|BXA1_CLOBO Botulinum neurotoxin type A precursor ... 29 7.9
>sp|Q35905|RMAR_SACDO Mitochondrial ribosomal protein VAR1
Length = 373
Score = 32.7 bits (73), Expect = 0.72
Identities = 21/78 (26%), Positives = 33/78 (42%)
Frame = -2
Query: 598 MPSLID*R**ININVLNNETKADNNQYSKTIKTFIHRNINFYNNETKLSNYNSCITRLKS 419
MP L D I++N +NN +NN+Y+ I + N N NN +NY I + +
Sbjct: 225 MPKLNDHN--ISMNYINNINNINNNKYNNMINLLNNNNNNNNNNNNNNNNYIDNINNIYN 282
Query: 418 YTVFSRIGLKFSFENILI 365
I + L+
Sbjct: 283 NMTIDNIPMDILMYKYLV 300
>sp|P46213|SYI_THEMA Isoleucyl-tRNA synthetase (Isoleucine--tRNA ligase) (IleRS)
Length = 919
Score = 31.6 bits (70), Expect = 1.6
Identities = 21/74 (28%), Positives = 39/74 (52%)
Frame = +3
Query: 249 NKNLIHENLYLLSIEQVIVHYCKSCRYQDIWSHHLNAIRINIFSNENFKPILENTV*LFN 428
++ L+ + LLSI + ++ + R QD+ H L+A + + N+ K +LE +
Sbjct: 794 DRKLMEDFEKLLSIREDVLKALEEKRQQDVIGHSLDAEVVLVPKNDTIKALLEK----YR 849
Query: 429 RVMHEL*LDSFVSL 470
++ EL + S VSL
Sbjct: 850 DILEELFIVSKVSL 863
>sp|Q03208|YML9_YEAST Hypothetical 40.1 kDa protein in NDI1-ATR1 intergenic region
Length = 357
Score = 30.4 bits (67), Expect = 3.6
Identities = 20/56 (35%), Positives = 34/56 (60%), Gaps = 3/56 (5%)
Frame = +1
Query: 280 SYRLSKSLYIIVNRVDIKISGLIISMQFESIYSQMKTLNLSL--KTQCS-SLIELC 438
+Y+L ++ ++ + KI L+IS++FE Y ++ + LS KT+ S SL ELC
Sbjct: 198 TYKLPSPVHETIDDISKKIIILLISLKFEKNYHFLQPIQLSTNSKTRISKSLDELC 253
>sp|P02381|RMAR_YEAST Mitochondrial ribosomal protein VAR1
Length = 404
Score = 30.4 bits (67), Expect = 3.6
Identities = 18/53 (33%), Positives = 28/53 (52%)
Frame = -2
Query: 598 MPSLID*R**ININVLNNETKADNNQYSKTIKTFIHRNINFYNNETKLSNYNS 440
MP L D I++N +NN +NN+Y+ I + N N NN ++N N+
Sbjct: 248 MPKLNDHN--ISMNYINNINNINNNKYNNMINLLNNNNNN--NNNNNINNNNN 296
>sp|P51842|GUC2F_RAT Retinal guanylyl cyclase 2 precursor (Guanylate cyclase 2F,
retinal) (RETGC-2) (Rod outer segment membrane guanylate
cyclase 2) (ROS-GC2) (Guanylate cyclase F) (GC-F)
Length = 1108
Score = 29.6 bits (65), Expect = 6.1
Identities = 12/35 (34%), Positives = 22/35 (62%)
Frame = -1
Query: 446 QFMHNSIKELHCVFKDRFKVFI*EYIDSNCIEMMR 342
+F+H +K +CV RF + + +Y +N +EM+R
Sbjct: 665 EFIHGRLKSRNCVVDGRFVLKVTDYGFNNILEMLR 699
>sp|O14265|VATH_SCHPO Vacuolar ATP synthase subunit H (V-ATPase H subunit) (Vacuolar
proton pump H subunit) (V-ATPase 54 kDa subunit)
Length = 450
Score = 29.3 bits (64), Expect = 7.9
Identities = 16/52 (30%), Positives = 29/52 (55%)
Frame = +3
Query: 216 LIYVRQ*LYDDNKNLIHENLYLLSIEQVIVHYCKSCRYQDIWSHHLNAIRIN 371
L ++ L + +K+L ++Y ++ I+H+ S R +D W H NA R+N
Sbjct: 314 LDFITSTLDESSKHLSTFDMYKSELDTGILHWSPSHRSEDFW--HQNAKRLN 363
>sp|P10845|BXA1_CLOBO Botulinum neurotoxin type A precursor (BoNT/A) (Bontoxilysin A)
(BOTOX) [Contains: Botulinum neurotoxin A light-chain;
Botulinum neurotoxin A heavy-chain]
Length = 1296
Score = 29.3 bits (64), Expect = 7.9
Identities = 24/68 (35%), Positives = 36/68 (52%), Gaps = 5/68 (7%)
Frame = +3
Query: 240 YDDNKNLIHENLYLLSIEQVIVHYCKSCRYQDIWSHHL-----NAIRINIFSNENFKPIL 404
Y DN+ L+ + + I+ +I + RY+ S+HL A +INI S NF PI
Sbjct: 856 YVDNQRLL--STFTEYIKNIINTSILNLRYE---SNHLIDLSRYASKINIGSKVNFDPID 910
Query: 405 ENTV*LFN 428
+N + LFN
Sbjct: 911 KNQIQLFN 918
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 61,631,121
Number of Sequences: 369166
Number of extensions: 1142579
Number of successful extensions: 2808
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 2633
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2784
length of database: 68,354,980
effective HSP length: 105
effective length of database: 48,957,805
effective search space used: 4602033670
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)