Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_009_A02
(719 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P81121|SP1_HORSE Seminal plasma protein HSP-1 44 6e-04
sp|P80964|PB1_PIG Seminal plasma protein pB1 precursor (pAI... 43 7e-04
sp|P81019|SFP4_BOVIN Seminal plasma protein BSP-30 kDa prec... 42 0.002
sp|P04557|SFP3_BOVIN Seminal plasma protein A3 precursor (B... 41 0.004
sp|Q90611|MMP2_CHICK 72 kDa type IV collagenase precursor (... 40 0.005
sp|Q07113|MPRI_MOUSE Cation-independent mannose-6-phosphate... 40 0.006
sp|P33434|MMP2_MOUSE 72 kDa type IV collagenase precursor (... 39 0.014
sp|P33436|MMP2_RAT 72 kDa type IV collagenase precursor (72... 39 0.014
sp|P08253|MMP2_HUMAN 72 kDa type IV collagenase precursor (... 38 0.031
sp|P08169|MPRI_BOVIN Cation-independent mannose-6-phosphate... 38 0.031
>sp|P81121|SP1_HORSE Seminal plasma protein HSP-1
Length = 120
Score = 43.5 bits (101), Expect = 6e-04
Identities = 17/43 (39%), Positives = 27/43 (62%)
Frame = +2
Query: 56 ECTFPFIYKGLLFEDCTDANADFLWCSPTKEYSGTSIPCSSIE 184
+C FPF Y+G + DCT ++ + WCS T YSG+ C++ +
Sbjct: 33 KCVFPFNYRGYRYYDCTRTDSFYRWCSLTGTYSGSWKYCAATD 75
Score = 35.0 bits (79), Expect = 0.20
Identities = 14/34 (41%), Positives = 20/34 (58%), Gaps = 2/34 (5%)
Frame = +2
Query: 56 ECTFPFIYKGLLFEDCTDANADF--LWCSPTKEY 151
+C FPF+Y+G ++ CT + F WCS T Y
Sbjct: 78 KCAFPFVYRGQTYDRCTTDGSLFRISWCSVTPNY 111
>sp|P80964|PB1_PIG Seminal plasma protein pB1 precursor (pAIF-1) (DQH protein)
Length = 130
Score = 43.1 bits (100), Expect = 7e-04
Identities = 17/32 (53%), Positives = 22/32 (68%)
Frame = +2
Query: 56 ECTFPFIYKGLLFEDCTDANADFLWCSPTKEY 151
+C FPFIYKG L+ DCT ++ + WCS T Y
Sbjct: 43 KCVFPFIYKGNLYFDCTLHDSTYYWCSVTTYY 74
>sp|P81019|SFP4_BOVIN Seminal plasma protein BSP-30 kDa precursor (BSP-30K)
Length = 183
Score = 42.0 bits (97), Expect = 0.002
Identities = 18/45 (40%), Positives = 24/45 (53%)
Frame = +2
Query: 59 CTFPFIYKGLLFEDCTDANADFLWCSPTKEYSGTSIPCSSIEDEQ 193
C FPF YKG + CT N+ LWCS EY G C+ ++ +
Sbjct: 97 CAFPFTYKGKKYYMCTRKNSVLLWCSLDTEYQGNWKFCTERDEPE 141
Score = 38.5 bits (88), Expect = 0.018
Identities = 19/43 (44%), Positives = 25/43 (58%), Gaps = 2/43 (4%)
Frame = +2
Query: 29 FSTKAGKPKECTFPFIYKGLLFEDCTDANADF--LWCSPTKEY 151
F T+ +P EC FPFIY+ +E CT ++ F WCS T Y
Sbjct: 133 FCTERDEP-ECVFPFIYRKKSYESCTRVHSFFWRRWCSLTSNY 174
>sp|P04557|SFP3_BOVIN Seminal plasma protein A3 precursor (BSP-A3)
Length = 140
Score = 40.8 bits (94), Expect = 0.004
Identities = 17/37 (45%), Positives = 22/37 (59%)
Frame = +2
Query: 47 KPKECTFPFIYKGLLFEDCTDANADFLWCSPTKEYSG 157
K +C FPFIY + DCT + FLWCS +Y+G
Sbjct: 50 KDNKCVFPFIYGNKKYFDCTLHGSLFLWCSLDADYTG 86
Score = 33.9 bits (76), Expect = 0.45
Identities = 19/58 (32%), Positives = 28/58 (48%), Gaps = 10/58 (17%)
Frame = +2
Query: 8 LLLSCVIFSTKAGKPKECT--------FPFIYKGLLFEDCTDANADFL--WCSPTKEY 151
L L C + + G+ K CT FPFIY+G ++ C + F+ WCS + Y
Sbjct: 74 LFLWCSLDADYTGRWKYCTKNDYAKCVFPFIYEGKSYDTCIIIGSTFMNYWCSLSSNY 131
>sp|Q90611|MMP2_CHICK 72 kDa type IV collagenase precursor (72 kDa gelatinase) (Matrix
metalloproteinase-2) (MMP-2) (Gelatinase A)
Length = 663
Score = 40.4 bits (93), Expect = 0.005
Identities = 17/33 (51%), Positives = 21/33 (63%), Gaps = 2/33 (6%)
Frame = +2
Query: 59 CTFPFIYKGLLFEDCTDA--NADFLWCSPTKEY 151
C FPF + G + CTDA N FLWCS TK++
Sbjct: 230 CKFPFWFNGKEYNSCTDAGRNDGFLWCSTTKDF 262
Score = 40.0 bits (92), Expect = 0.006
Identities = 26/78 (33%), Positives = 37/78 (47%), Gaps = 4/78 (5%)
Frame = +2
Query: 59 CTFPFIYKGLLFEDCTDA--NADFLWCSPTKEYSGTSIPCSSIEDEQR--LKRDNYYQLF 226
C FPFI+ G ++ CT A N LWC+ T SS +D+++ D Y LF
Sbjct: 346 CVFPFIFLGNKYDSCTSAGRNDGKLWCAST----------SSYDDDRKWGFCPDQGYSLF 395
Query: 227 KVLEEQEAHIKKLEASQE 280
V + H LE S++
Sbjct: 396 LVAAHEFGHAMGLEHSED 413
Score = 32.0 bits (71), Expect = 1.7
Identities = 13/38 (34%), Positives = 22/38 (57%), Gaps = 2/38 (5%)
Frame = +2
Query: 44 GKPKECTFPFIYKGLLFEDC-TDANAD-FLWCSPTKEY 151
G + C FPF ++G ++ C T+ D + WC T++Y
Sbjct: 283 GDGQPCKFPFKFQGQSYDQCTTEGRTDGYRWCGTTEDY 320
>sp|Q07113|MPRI_MOUSE Cation-independent mannose-6-phosphate receptor precursor (CI Man-6-P
receptor) (CI-MPR) (M6PR) (Insulin-like growth factor II
receptor) (300 kDa mannose 6-phosphate receptor) (MPR
300) (MPR300)
Length = 2483
Score = 40.0 bits (92), Expect = 0.006
Identities = 18/39 (46%), Positives = 22/39 (56%)
Frame = +2
Query: 35 TKAGKPKECTFPFIYKGLLFEDCTDANADFLWCSPTKEY 151
T G+P C FPFIYKG +++C LWCS T Y
Sbjct: 1890 TDDGEP--CVFPFIYKGKSYDECVLEGRAKLWCSKTANY 1926
>sp|P33434|MMP2_MOUSE 72 kDa type IV collagenase precursor (72 kDa gelatinase) (Matrix
metalloproteinase-2) (MMP-2) (Gelatinase A)
Length = 662
Score = 38.9 bits (89), Expect = 0.014
Identities = 25/78 (32%), Positives = 35/78 (44%), Gaps = 4/78 (5%)
Frame = +2
Query: 59 CTFPFIYKGLLFEDCTDA--NADFLWCSPTKEYSGTSIPCSSIEDEQR--LKRDNYYQLF 226
C FPF + G +E CT A N +WC+ T Y +D+++ D Y LF
Sbjct: 349 CVFPFTFLGNKYESCTSAGRNDGKVWCATTTNY----------DDDRKWGFCPDQGYSLF 398
Query: 227 KVLEEQEAHIKKLEASQE 280
V + H LE SQ+
Sbjct: 399 LVAAHEFGHAMGLEHSQD 416
Score = 35.8 bits (81), Expect = 0.12
Identities = 14/33 (42%), Positives = 18/33 (54%), Gaps = 2/33 (6%)
Frame = +2
Query: 59 CTFPFIYKGLLFEDCTDANAD--FLWCSPTKEY 151
C FPF++ G + CTD FLWCS T +
Sbjct: 233 CKFPFLFNGREYSSCTDTGRSDGFLWCSTTYNF 265
Score = 30.8 bits (68), Expect = 3.8
Identities = 14/38 (36%), Positives = 22/38 (57%), Gaps = 2/38 (5%)
Frame = +2
Query: 44 GKPKECTFPFIYKGLLFEDC-TDANAD-FLWCSPTKEY 151
G+P C FPF ++G + C T+ D + WC T++Y
Sbjct: 288 GQP--CKFPFRFQGTSYNSCTTEGRTDGYRWCGTTEDY 323
>sp|P33436|MMP2_RAT 72 kDa type IV collagenase precursor (72 kDa gelatinase) (Matrix
metalloproteinase-2) (MMP-2) (Gelatinase A)
Length = 662
Score = 38.9 bits (89), Expect = 0.014
Identities = 25/78 (32%), Positives = 35/78 (44%), Gaps = 4/78 (5%)
Frame = +2
Query: 59 CTFPFIYKGLLFEDCTDA--NADFLWCSPTKEYSGTSIPCSSIEDEQR--LKRDNYYQLF 226
C FPF + G +E CT A N +WC+ T Y +D+++ D Y LF
Sbjct: 349 CVFPFTFLGNKYESCTSAGRNDGKVWCATTTNY----------DDDRKWGFCPDQGYSLF 398
Query: 227 KVLEEQEAHIKKLEASQE 280
V + H LE SQ+
Sbjct: 399 LVAAHEFGHAMGLEHSQD 416
Score = 35.8 bits (81), Expect = 0.12
Identities = 14/33 (42%), Positives = 18/33 (54%), Gaps = 2/33 (6%)
Frame = +2
Query: 59 CTFPFIYKGLLFEDCTDANAD--FLWCSPTKEY 151
C FPF++ G + CTD FLWCS T +
Sbjct: 233 CKFPFLFNGREYSSCTDTGRSDGFLWCSTTYNF 265
Score = 30.8 bits (68), Expect = 3.8
Identities = 14/38 (36%), Positives = 22/38 (57%), Gaps = 2/38 (5%)
Frame = +2
Query: 44 GKPKECTFPFIYKGLLFEDC-TDANAD-FLWCSPTKEY 151
G+P C FPF ++G + C T+ D + WC T++Y
Sbjct: 288 GQP--CKFPFRFQGTSYNSCTTEGRTDGYRWCGTTEDY 323
>sp|P08253|MMP2_HUMAN 72 kDa type IV collagenase precursor (72 kDa gelatinase) (Matrix
metalloproteinase-2) (MMP-2) (Gelatinase A) (TBE-1)
Length = 660
Score = 37.7 bits (86), Expect = 0.031
Identities = 24/78 (30%), Positives = 34/78 (43%), Gaps = 4/78 (5%)
Frame = +2
Query: 59 CTFPFIYKGLLFEDCTDANAD--FLWCSPTKEYSGTSIPCSSIEDEQR--LKRDNYYQLF 226
C FPF + G +E CT A +WC+ T Y +D+++ D Y LF
Sbjct: 349 CVFPFTFLGNKYESCTSAGRSDGKMWCATTANY----------DDDRKWGFCPDQGYSLF 398
Query: 227 KVLEEQEAHIKKLEASQE 280
V + H LE SQ+
Sbjct: 399 LVAAHEFGHAMGLEHSQD 416
Score = 35.8 bits (81), Expect = 0.12
Identities = 14/33 (42%), Positives = 18/33 (54%), Gaps = 2/33 (6%)
Frame = +2
Query: 59 CTFPFIYKGLLFEDCTDANAD--FLWCSPTKEY 151
C FPF++ G + CTD FLWCS T +
Sbjct: 233 CKFPFLFNGKEYNSCTDTGRSDGFLWCSTTYNF 265
Score = 31.6 bits (70), Expect = 2.2
Identities = 14/38 (36%), Positives = 23/38 (60%), Gaps = 2/38 (5%)
Frame = +2
Query: 44 GKPKECTFPFIYKGLLFEDC-TDANAD-FLWCSPTKEY 151
G+P C FPF ++G ++ C T+ D + WC T++Y
Sbjct: 288 GQP--CKFPFRFQGTSYDSCTTEGRTDGYRWCGTTEDY 323
>sp|P08169|MPRI_BOVIN Cation-independent mannose-6-phosphate receptor precursor (CI Man-6-P
receptor) (CI-MPR) (M6PR) (Insulin-like growth factor II
receptor) (300 kDa mannose 6-phosphate receptor) (MPR
300) (MPR300)
Length = 2499
Score = 37.7 bits (86), Expect = 0.031
Identities = 15/39 (38%), Positives = 23/39 (58%)
Frame = +2
Query: 35 TKAGKPKECTFPFIYKGLLFEDCTDANADFLWCSPTKEY 151
T+ G+P C FPF++ G +E+C + LWC+ T Y
Sbjct: 1906 TEDGEP--CVFPFVFNGKSYEECVVESRARLWCATTANY 1942
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 78,364,669
Number of Sequences: 369166
Number of extensions: 1495502
Number of successful extensions: 4194
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4015
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4189
length of database: 68,354,980
effective HSP length: 107
effective length of database: 48,588,335
effective search space used: 6413660220
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)