Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_008_P10
(210 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O13035|SAP_CHICK Proactivator polypeptide precursor [Con... 34 0.12
sp|Q5DU02|UBP22_MOUSE Ubiquitin carboxyl-terminal hydrolase... 31 1.0
sp|P50405|PSPB_MOUSE Pulmonary surfactant-associated protei... 31 1.0
sp|Q9UPT9|UBP22_HUMAN Ubiquitin carboxyl-terminal hydrolase... 31 1.0
sp|Q6EW41|CEMA_NYMAL Chloroplast envelope membrane protein 30 1.7
sp|P07602|SAP_HUMAN Proactivator polypeptide precursor [Con... 29 2.9
sp|P09810|TPH1_RAT Tryptophan 5-hydroxylase 1 (Tryptophan 5... 28 6.5
sp|P17532|TPH1_MOUSE Tryptophan 5-hydroxylase 1 (Tryptophan... 28 6.5
sp|P70080|TPH1_CHICK Tryptophan 5-hydroxylase 1 (Tryptophan... 28 6.5
sp|P26779|SAP_BOVIN Proactivator polypeptide precursor [Con... 28 6.5
>sp|O13035|SAP_CHICK Proactivator polypeptide precursor [Contains: Saposin A; Saposin
B; Saposin C; Saposin D]
Length = 518
Score = 33.9 bits (76), Expect = 0.12
Identities = 14/24 (58%), Positives = 16/24 (66%)
Frame = +2
Query: 5 WCVSRKHAVACGPGAVKHCEMNVW 76
WC S + A CG AVKHC+ NVW
Sbjct: 33 WCQSLRTASQCG--AVKHCQQNVW 54
>sp|Q5DU02|UBP22_MOUSE Ubiquitin carboxyl-terminal hydrolase 22 (Ubiquitin thiolesterase
22) (Ubiquitin-specific processing protease 22)
(Deubiquitinating enzyme 22)
Length = 525
Score = 30.8 bits (68), Expect = 1.0
Identities = 15/40 (37%), Positives = 22/40 (55%), Gaps = 3/40 (7%)
Frame = -3
Query: 148 CELKNSSSL---KSSIRIVELYNGHLSPHIHFTMFHCTGT 38
CE+++ SS + S E Y+GH SPHI + + H T
Sbjct: 211 CEMQSPSSCLVCEMSSLFQEFYSGHRSPHIPYKLLHLVWT 250
>sp|P50405|PSPB_MOUSE Pulmonary surfactant-associated protein B precursor (SP-B)
(Pulmonary surfactant-associated proteolipid SPL(Phe))
Length = 377
Score = 30.8 bits (68), Expect = 1.0
Identities = 13/25 (52%), Positives = 15/25 (60%)
Frame = +2
Query: 2 FWCVSRKHAVACGPGAVKHCEMNVW 76
FWC S +HAV C A+ HC VW
Sbjct: 37 FWCQSLEHAVQC--RALGHCLQEVW 59
>sp|Q9UPT9|UBP22_HUMAN Ubiquitin carboxyl-terminal hydrolase 22 (Ubiquitin thiolesterase
22) (Ubiquitin-specific processing protease 22)
(Deubiquitinating enzyme 22)
Length = 525
Score = 30.8 bits (68), Expect = 1.0
Identities = 15/40 (37%), Positives = 22/40 (55%), Gaps = 3/40 (7%)
Frame = -3
Query: 148 CELKNSSSL---KSSIRIVELYNGHLSPHIHFTMFHCTGT 38
CE+++ SS + S E Y+GH SPHI + + H T
Sbjct: 211 CEMQSPSSCLVCEMSSLFQEFYSGHRSPHIPYKLLHLVWT 250
>sp|Q6EW41|CEMA_NYMAL Chloroplast envelope membrane protein
Length = 229
Score = 30.0 bits (66), Expect = 1.7
Identities = 12/38 (31%), Positives = 22/38 (57%)
Frame = -3
Query: 121 KSSIRIVELYNGHLSPHIHFTMFHCTGTTCYSVFPAYT 8
K +I++V ++N HIH + T TC+++ AY+
Sbjct: 90 KETIQLVRMHN---QDHIHIILHFSTNITCFAILSAYS 124
>sp|P07602|SAP_HUMAN Proactivator polypeptide precursor [Contains: Saposin A (Protein
A); Saposin B-Val; Saposin B (Sphingolipid activator
protein 1) (SAP-1) (Cerebroside sulfate activator)
(CSAct) (Dispersin) (Sulfatide/GM1 activator); Saposin C
(Co-beta-glucosidase) (A1 activator) (Glucosylceramidase
activator) (Sphingolipid activator protein 2) (SAP-2);
Saposin D (Protein C) (Component C)]
Length = 524
Score = 29.3 bits (64), Expect = 2.9
Identities = 13/26 (50%), Positives = 15/26 (57%)
Frame = +2
Query: 5 WCVSRKHAVACGPGAVKHCEMNVWRQ 82
WC + K A CG AVKHC VW +
Sbjct: 32 WCQNVKTASDCG--AVKHCLQTVWNK 55
Score = 28.1 bits (61), Expect = 6.5
Identities = 10/25 (40%), Positives = 16/25 (64%)
Frame = +2
Query: 2 FWCVSRKHAVACGPGAVKHCEMNVW 76
+WC + + A C AV+HC+ +VW
Sbjct: 501 YWCQNTETAAQCN--AVEHCKRHVW 523
>sp|P09810|TPH1_RAT Tryptophan 5-hydroxylase 1 (Tryptophan 5-monooxygenase 1)
Length = 444
Score = 28.1 bits (61), Expect = 6.5
Identities = 14/34 (41%), Positives = 19/34 (55%), Gaps = 5/34 (14%)
Frame = -3
Query: 88 GHLSPH-----IHFTMFHCTGTTCYSVFPAYTPK 2
G+LSP + F +FHCT +S P YTP+
Sbjct: 234 GYLSPRDFLSGLAFRVFHCTQYVRHSSDPLYTPE 267
>sp|P17532|TPH1_MOUSE Tryptophan 5-hydroxylase 1 (Tryptophan 5-monooxygenase 1)
Length = 447
Score = 28.1 bits (61), Expect = 6.5
Identities = 14/34 (41%), Positives = 19/34 (55%), Gaps = 5/34 (14%)
Frame = -3
Query: 88 GHLSPH-----IHFTMFHCTGTTCYSVFPAYTPK 2
G+LSP + F +FHCT +S P YTP+
Sbjct: 237 GYLSPRDFLSGLAFRVFHCTQYVRHSSDPLYTPE 270
>sp|P70080|TPH1_CHICK Tryptophan 5-hydroxylase 1 (Tryptophan 5-monooxygenase 1)
Length = 445
Score = 28.1 bits (61), Expect = 6.5
Identities = 14/34 (41%), Positives = 19/34 (55%), Gaps = 5/34 (14%)
Frame = -3
Query: 88 GHLSPH-----IHFTMFHCTGTTCYSVFPAYTPK 2
G+LSP + F +FHCT +S P YTP+
Sbjct: 235 GYLSPRDFLAGLAFRVFHCTQYVRHSSDPLYTPE 268
>sp|P26779|SAP_BOVIN Proactivator polypeptide precursor [Contains: Saposin A (Protein
A); Saposin B (Sphingolipid activator protein 1)
(SAP-1) (Cerebroside sulfate activator) (CSAct)
(Dispersin) (Sulfatide/GM1 activator); Saposin C
(Co-beta-glucosidase) (A1 activator)
(Glucosylceramidase activator) (Sphingolipid activator
protein 2) (SAP-2); Saposin D (Protein C) (Component
C)]
Length = 525
Score = 28.1 bits (61), Expect = 6.5
Identities = 12/24 (50%), Positives = 14/24 (58%)
Frame = +2
Query: 5 WCVSRKHAVACGPGAVKHCEMNVW 76
WC + K A CG AV+HC VW
Sbjct: 32 WCQNVKTAADCG--AVQHCLQTVW 53
Score = 28.1 bits (61), Expect = 6.5
Identities = 10/25 (40%), Positives = 15/25 (60%)
Frame = +2
Query: 2 FWCVSRKHAVACGPGAVKHCEMNVW 76
+WC + + A C AV+HC +VW
Sbjct: 502 YWCQNMESAALCN--AVEHCRRHVW 524
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 21,908,106
Number of Sequences: 369166
Number of extensions: 284325
Number of successful extensions: 666
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 661
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 666
length of database: 68,354,980
effective HSP length: 41
effective length of database: 60,780,845
effective search space used: 1701863660
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)