Planarian EST Database


Dr_sW_008_O19

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_008_O19
         (403 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q7WRG7|MUTS_STAAU  DNA mismatch repair protein mutS >gi|6...    37   0.011
sp|Q6GHE0|MUTS_STAAR  DNA mismatch repair protein mutS             35   0.042
sp|Q9PPB8|SYC_CAMJE  Cysteinyl-tRNA synthetase (Cysteine--tR...    32   0.47 
sp|P27986|P85A_HUMAN  Phosphatidylinositol 3-kinase regulato...    32   0.61 
sp|Q63787|P85A_RAT  Phosphatidylinositol 3-kinase regulatory...    32   0.61 
sp|P26450|P85A_MOUSE  Phosphatidylinositol 3-kinase regulato...    32   0.61 
sp|Q05755|GLTB_AZOBR  Glutamate synthase [NADPH] large chain...    31   0.80 
sp|Q5HUZ0|SYC_CAMJR  Cysteinyl-tRNA synthetase (Cysteine--tR...    31   0.80 
sp|Q9CNU1|GRPE_PASMU  GrpE protein (HSP-70 cofactor)               31   0.80 
sp|P23727|P85A_BOVIN  Phosphatidylinositol 3-kinase regulato...    31   1.0  
>sp|Q7WRG7|MUTS_STAAU DNA mismatch repair protein mutS
 sp|Q5HGD6|MUTS_STAAC DNA mismatch repair protein mutS
 sp|P65494|MUTS_STAAW DNA mismatch repair protein mutS
 sp|Q6G9R8|MUTS_STAAS DNA mismatch repair protein mutS
 sp|P65493|MUTS_STAAN DNA mismatch repair protein mutS
 sp|Q931S8|MUTS_STAAM DNA mismatch repair protein mutS
          Length = 872

 Score = 37.4 bits (85), Expect = 0.011
 Identities = 16/49 (32%), Positives = 29/49 (59%)
 Frame = +3

Query: 111 LEEVVREYTRAYEQETRLLQAKGDIRTRAETAKLYQYTTPSFMRREALQ 257
           L E V++YT   +Q+ +++     +++ AE A+ Y YT PSF   + L+
Sbjct: 518 LREEVKKYTERLQQQAKIISELDCLQSFAEIAQKYNYTRPSFSENKTLE 566
>sp|Q6GHE0|MUTS_STAAR DNA mismatch repair protein mutS
          Length = 872

 Score = 35.4 bits (80), Expect = 0.042
 Identities = 15/49 (30%), Positives = 28/49 (57%)
 Frame = +3

Query: 111 LEEVVREYTRAYEQETRLLQAKGDIRTRAETAKLYQYTTPSFMRREALQ 257
           L E V++YT   + + +++     +++ AE A+ Y YT PSF   + L+
Sbjct: 518 LREEVKKYTERLQHQAKIISELDCLQSFAEIAQKYNYTRPSFSENKTLE 566
>sp|Q9PPB8|SYC_CAMJE Cysteinyl-tRNA synthetase (Cysteine--tRNA ligase) (CysRS)
          Length = 462

 Score = 32.0 bits (71), Expect = 0.47
 Identities = 15/46 (32%), Positives = 27/46 (58%), Gaps = 1/46 (2%)
 Frame = +3

Query: 63  NIDEHFKRKLITSNEPLEEVVREYTRAYEQETRLLQA-KGDIRTRA 197
           +ID+   +K+  S + LEE+   Y ++YE++ R+L     D + RA
Sbjct: 65  DIDDKILKKMAQSGQTLEEITEFYIKSYEEDMRVLNVLDPDFKPRA 110
>sp|P27986|P85A_HUMAN Phosphatidylinositol 3-kinase regulatory alpha subunit (PI3-kinase
           p85-alpha subunit) (PtdIns-3-kinase p85-alpha) (PI3K)
          Length = 724

 Score = 31.6 bits (70), Expect = 0.61
 Identities = 21/63 (33%), Positives = 32/63 (50%), Gaps = 4/63 (6%)
 Frame = +3

Query: 42  QEKMKKENIDEHFKRKLITSNEPLEEVVREYTRAYEQETRLLQ----AKGDIRTRAETAK 209
           Q+++ KE+  E   +KL   N   +E  REY R YE+ TR  Q     +  I    ET K
Sbjct: 433 QDQVVKEDNIEAVGKKLHEYNTQFQEKSREYDRLYEEYTRTSQEIQMKRTAIEAFNETIK 492

Query: 210 LYQ 218
           +++
Sbjct: 493 IFE 495
>sp|Q63787|P85A_RAT Phosphatidylinositol 3-kinase regulatory alpha subunit (PI3-kinase
           p85-alpha subunit) (PtdIns-3-kinase p85-alpha) (PI3K)
          Length = 724

 Score = 31.6 bits (70), Expect = 0.61
 Identities = 22/63 (34%), Positives = 32/63 (50%), Gaps = 4/63 (6%)
 Frame = +3

Query: 42  QEKMKKENIDEHFKRKLITSNEPLEEVVREYTRAYEQETRLLQAKGDIRTRAE----TAK 209
           Q+++ KE+  E   +KL   N   +E  REY R YE+ TR  Q     RT  E    T K
Sbjct: 433 QDQVVKEDNIEAVGKKLHEYNTQFQEKSREYDRLYEEYTRTSQEIQMKRTAIEAFNDTIK 492

Query: 210 LYQ 218
           +++
Sbjct: 493 IFE 495
>sp|P26450|P85A_MOUSE Phosphatidylinositol 3-kinase regulatory alpha subunit (PI3-kinase
           p85-alpha subunit) (PtdIns-3-kinase p85-alpha) (PI3K)
          Length = 724

 Score = 31.6 bits (70), Expect = 0.61
 Identities = 21/63 (33%), Positives = 32/63 (50%), Gaps = 4/63 (6%)
 Frame = +3

Query: 42  QEKMKKENIDEHFKRKLITSNEPLEEVVREYTRAYEQETRLLQ----AKGDIRTRAETAK 209
           Q+++ KE+  E   +KL   N   +E  REY R YE+ TR  Q     +  I    ET K
Sbjct: 433 QDQVVKEDNIEAVGKKLHEYNTQFQEKSREYDRLYEEYTRTSQEIQMKRTAIEAFNETIK 492

Query: 210 LYQ 218
           +++
Sbjct: 493 IFE 495
>sp|Q05755|GLTB_AZOBR Glutamate synthase [NADPH] large chain precursor (Glutamate
           synthase alpha subunit) (NADPH-GOGAT) (GLTS alpha chain)
          Length = 1515

 Score = 31.2 bits (69), Expect = 0.80
 Identities = 19/64 (29%), Positives = 29/64 (45%)
 Frame = +3

Query: 147 EQETRLLQAKGDIRTRAETAKLYQYTTPSFMRREALQPNTHSDLKSEVLIRTLRKPTEGL 326
           E +TRLLQ +  + T AE   +  Y   +    +A  P           +R +R+ TE  
Sbjct: 573 ETQTRLLQLESPVLTTAEFRAMRDYMGDTAAEIDATFPVDGGPEALRDALRRIRQETEDA 632

Query: 327 IRGG 338
           +RGG
Sbjct: 633 VRGG 636
>sp|Q5HUZ0|SYC_CAMJR Cysteinyl-tRNA synthetase (Cysteine--tRNA ligase) (CysRS)
          Length = 462

 Score = 31.2 bits (69), Expect = 0.80
 Identities = 15/46 (32%), Positives = 26/46 (56%), Gaps = 1/46 (2%)
 Frame = +3

Query: 63  NIDEHFKRKLITSNEPLEEVVREYTRAYEQETRLLQA-KGDIRTRA 197
           +ID+   +K+  S + LEE+   Y ++YE++ R L     D + RA
Sbjct: 65  DIDDKILKKMTQSGQTLEEITEFYIKSYEEDMRALNVLDPDFKPRA 110
>sp|Q9CNU1|GRPE_PASMU GrpE protein (HSP-70 cofactor)
          Length = 197

 Score = 31.2 bits (69), Expect = 0.80
 Identities = 15/64 (23%), Positives = 35/64 (54%), Gaps = 2/64 (3%)
 Frame = +3

Query: 15  HLQVNDEMNQEKMKKENIDEHFKRKLITSNEPLEEVVREYTRAYEQETRLLQAKGD--IR 188
           H++  +++ +EK +++   E  + + +   +PLEE +        Q T +++ + D  +R
Sbjct: 7   HIEKEEQLQEEKHEEQQKTEETEVEAVNGVDPLEEAILRVQELEAQLTEMVKKEQDFLLR 66

Query: 189 TRAE 200
           +RAE
Sbjct: 67  SRAE 70
>sp|P23727|P85A_BOVIN Phosphatidylinositol 3-kinase regulatory alpha subunit (PI3-kinase
           p85-alpha subunit) (PtdIns-3-kinase p85-alpha) (PI3K)
          Length = 724

 Score = 30.8 bits (68), Expect = 1.0
 Identities = 21/63 (33%), Positives = 31/63 (49%), Gaps = 4/63 (6%)
 Frame = +3

Query: 42  QEKMKKENIDEHFKRKLITSNEPLEEVVREYTRAYEQETRLLQ----AKGDIRTRAETAK 209
           Q+++ KE+  E   +KL   N   +E  REY R YE  TR  Q     +  I    ET K
Sbjct: 433 QDQVVKEDNIEAVGKKLHEYNTQFQEKSREYDRLYEDYTRTSQEIQMKRTAIEAFNETIK 492

Query: 210 LYQ 218
           +++
Sbjct: 493 IFE 495
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 47,641,048
Number of Sequences: 369166
Number of extensions: 939703
Number of successful extensions: 3043
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2918
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3036
length of database: 68,354,980
effective HSP length: 98
effective length of database: 50,250,950
effective search space used: 1758783250
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)