Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_008_O05
(907 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P07602|SAP_HUMAN Proactivator polypeptide precursor [Con... 78 3e-14
sp|P26779|SAP_BOVIN Proactivator polypeptide precursor [Con... 78 3e-14
sp|O13035|SAP_CHICK Proactivator polypeptide precursor [Con... 70 1e-11
sp|Q61207|SAP_MOUSE Sulfated glycoprotein 1 precursor (SGP-... 54 6e-07
sp|P50405|PSPB_MOUSE Pulmonary surfactant-associated protei... 54 8e-07
sp|P10960|SAP_RAT Sulfated glycoprotein 1 precursor (SGP-1)... 52 2e-06
sp|P15285|PSPB_RABIT Pulmonary surfactant-associated protei... 51 4e-06
sp|P20097|SAP_CAVPO Saposin C (CO-beta-glucosidase) (Glucos... 49 1e-05
sp|P22355|PSPB_RAT Pulmonary surfactant-associated protein ... 49 2e-05
sp|P07988|PSPB_HUMAN Pulmonary surfactant-associated protei... 42 0.002
>sp|P07602|SAP_HUMAN Proactivator polypeptide precursor [Contains: Saposin A (Protein A);
Saposin B-Val; Saposin B (Sphingolipid activator protein
1) (SAP-1) (Cerebroside sulfate activator) (CSAct)
(Dispersin) (Sulfatide/GM1 activator); Saposin C
(Co-beta-glucosidase) (A1 activator) (Glucosylceramidase
activator) (Sphingolipid activator protein 2) (SAP-2);
Saposin D (Protein C) (Component C)]
Length = 524
Score = 78.2 bits (191), Expect = 3e-14
Identities = 58/287 (20%), Positives = 111/287 (38%), Gaps = 31/287 (10%)
Frame = +2
Query: 53 CQTCLDVVVMLKKDMVNVPASEVEALIKN--NICAKLNFVAKPICNDYVDNYVPKFMSEI 226
CQ C+ +V ++ V ++ V+AL+++ C +L IC +Y+ Y + ++
Sbjct: 198 CQDCIQMVTDIQT-AVRTNSTFVQALVEHVKEECDRLGPGMADICKNYISQY-SEIAIQM 255
Query: 227 QSTMTPQQICNTNHFC--------------------------MAPSIPKPNVDMKGPVTC 328
M P++IC FC + I K V K V C
Sbjct: 256 MMHMQPKEICALVGFCDEVKEMPMQTLVPAKVASKNVIPALELVEPIKKHEVPAKSDVYC 315
Query: 329 NLCEIAVSEMKSFISNTENXXXXXXXXXXXXXXXXXXXXXXXXXVVDRYGDILMTFIINH 508
+CE V E+ I N + VVD YG +++ ++
Sbjct: 316 EVCEFLVKEVTKLIDNNKTEKEILDAFDKMCSKLPKSLSEECQEVVDTYGSSILSILLEE 375
Query: 509 MDAKSICKLIHACTSVNVQLKPIRKMK---GIECDACRLVVKKFKEYISDPATETEIKNF 679
+ + +C ++H C+ + + + G C+ C+ +V + +T+ EI
Sbjct: 376 VSPELVCSMLHLCSGTRLPALTVHVTQPKDGGFCEVCKKLVGYLDRNLEKNSTKQEILAA 435
Query: 680 LKNDFCYIFGSDKIQCQQIVDEYFNLILNFILAEVDVDKICKFFGLC 820
L+ ++ + QC Q V EY +++ ++ +D +C G C
Sbjct: 436 LEKGCSFLPDPYQKQCDQFVAEYEPVLIEILVEVMDPSFVCLKIGAC 482
Score = 58.9 bits (141), Expect = 2e-08
Identities = 71/337 (21%), Positives = 125/337 (37%), Gaps = 74/337 (21%)
Frame = +2
Query: 35 TLNSWNCQTCLDVVVMLKKDMVNVPASEVEALIKNNICAKLNFVAKP----ICNDYVDNY 202
T+ S C C DVV DM+ A+E E L+ + +++ KP C + VD+Y
Sbjct: 57 TVKSLPCDICKDVVTAAG-DMLKDNATEEEILVY--LEKTCDWLPKPNMSASCKEIVDSY 113
Query: 203 VPKFMSEIQSTMT-PQQICNT--------------NHFCMAPSIPKPNVDMKGPVT---- 325
+P + I+ M+ P ++C+ NH S P +DM V
Sbjct: 114 LPVILDIIKGEMSRPGEVCSALNLCESLQKHLAELNHQKQLESNKIPELDMTEVVAPFMA 173
Query: 326 ------------------------CNLCEIAVSEMKSFI-SNTENXXXXXXXXXXXXXXX 430
C C V+++++ + +N+
Sbjct: 174 NIPLLLYPQDGPRSKPQPKDNGDVCQDCIQMVTDIQTAVRTNSTFVQALVEHVKEECDRL 233
Query: 431 XXXXXXXXXXVVDRYGDILMTFIINHMDAKSICKLIHACTSVNVQ--------------- 565
+ +Y +I + ++ HM K IC L+ C V
Sbjct: 234 GPGMADICKNYISQYSEIAIQMMM-HMQPKEICALVGFCDEVKEMPMQTLVPAKVASKNV 292
Query: 566 ------LKPIRKMK-----GIECDACRLVVKKFKEYISDPATETEIKNFLKNDFCYIFGS 712
++PI+K + + C+ C +VK+ + I + TE EI + + S
Sbjct: 293 IPALELVEPIKKHEVPAKSDVYCEVCEFLVKEVTKLIDNNKTEKEILDAFDKMCSKLPKS 352
Query: 713 DKIQCQQIVDEYFNLILNFILAEVDVDKICKFFGLCN 823
+CQ++VD Y + IL+ +L EV + +C LC+
Sbjct: 353 LSEECQEVVDTYGSSILSILLEEVSPELVCSMLHLCS 389
Score = 46.2 bits (108), Expect = 1e-04
Identities = 36/176 (20%), Positives = 66/176 (37%), Gaps = 4/176 (2%)
Frame = +2
Query: 53 CQTCLDVVVMLKKDMVNVPASEVEALIK-NNICAKLNFVAKPICNDYVDNYVPKFMSEIQ 229
C+ C +V + K +++ +E E L + +C+KL C + VD Y +S +
Sbjct: 315 CEVCEFLVKEVTK-LIDNNKTEKEILDAFDKMCSKLPKSLSEECQEVVDTYGSSILSILL 373
Query: 230 STMTPQQICNTNHFCMAPSIPKPNVDMKGPVT---CNLCEIAVSEMKSFISNTENXXXXX 400
++P+ +C+ H C +P V + P C +C+ V + +
Sbjct: 374 EEVSPELVCSMLHLCSGTRLPALTVHVTQPKDGGFCEVCKKLVGYLDRNLEKNSTKQEIL 433
Query: 401 XXXXXXXXXXXXXXXXXXXXVVDRYGDILMTFIINHMDAKSICKLIHACTSVNVQL 568
V Y +L+ ++ MD +C I AC S + L
Sbjct: 434 AALEKGCSFLPDPYQKQCDQFVAEYEPVLIEILVEVMDPSFVCLKIGACPSAHKPL 489
Score = 43.1 bits (100), Expect = 0.001
Identities = 25/83 (30%), Positives = 43/83 (51%), Gaps = 2/83 (2%)
Frame = +2
Query: 584 MKGIECDACRLVVKKFKEYISDPATETEIKNFLKNDFCYIFGSD-KIQCQQIVDEYFNLI 760
+K + CD C+ VV + + D ATE EI +L+ ++ + C++IVD Y +I
Sbjct: 58 VKSLPCDICKDVVTAAGDMLKDNATEEEILVYLEKTCDWLPKPNMSASCKEIVDSYLPVI 117
Query: 761 LNFILAEVD-VDKICKFFGLCNA 826
L+ I E+ ++C LC +
Sbjct: 118 LDIIKGEMSRPGEVCSALNLCES 140
>sp|P26779|SAP_BOVIN Proactivator polypeptide precursor [Contains: Saposin A (Protein A);
Saposin B (Sphingolipid activator protein 1) (SAP-1)
(Cerebroside sulfate activator) (CSAct) (Dispersin)
(Sulfatide/GM1 activator); Saposin C
(Co-beta-glucosidase) (A1 activator) (Glucosylceramidase
activator) (Sphingolipid activator protein 2) (SAP-2);
Saposin D (Protein C) (Component C)]
Length = 525
Score = 78.2 bits (191), Expect = 3e-14
Identities = 58/289 (20%), Positives = 112/289 (38%), Gaps = 31/289 (10%)
Frame = +2
Query: 53 CQTCLDVVVMLKKDMVNVPASEVEALIKN--NICAKLNFVAKPICNDYVDNYVPKFMSEI 226
CQ C+ +V ++ + + ++ VEAL+ + C +L +C +Y++ Y + +
Sbjct: 199 CQDCIQLVTDVQ-EALRTNSTFVEALVDHAKEECDRLGPGMSDMCKNYINQYSEVAIQMV 257
Query: 227 QSTMTPQQICNTNHFC--------------------------MAPSIPKPNVDMKGPVTC 328
M P++IC FC + I K K + C
Sbjct: 258 MH-MQPKEICVLAGFCDEVKEMPMKTLVPAEVVSENVIPALGLVEPIKKDPAPAKADIYC 316
Query: 329 NLCEIAVSEMKSFISNTENXXXXXXXXXXXXXXXXXXXXXXXXXVVDRYGDILMTFIINH 508
+CE V E+ I N VVD YG +++ +++
Sbjct: 317 EVCEFVVKEVAKLIDNNRTEEEILHALDKVCSKLPTSLAEQCQEVVDTYGRSILSILLDE 376
Query: 509 MDAKSICKLIHACTSVNVQLKPIRKMK---GIECDACRLVVKKFKEYISDPATETEIKNF 679
+ +C ++H C+S + +R M G C+ C+ +V + +T+ +I
Sbjct: 377 ASPELVCSMLHLCSSRGLPAATVRVMPRKDGGFCEVCKKLVGYLDRNLEKNSTKEQILAA 436
Query: 680 LKNDFCYIFGSDKIQCQQIVDEYFNLILNFILAEVDVDKICKFFGLCNA 826
L+ ++ + QC Q V EY +++ ++ +D +C G C A
Sbjct: 437 LEKGCSFLPDQYRKQCDQFVTEYEPVLIEILVEVMDPSFVCLKIGACPA 485
Score = 55.5 bits (132), Expect = 2e-07
Identities = 75/378 (19%), Positives = 138/378 (36%), Gaps = 87/378 (23%)
Frame = +2
Query: 35 TLNSWNCQTCLDVVVMLKKDMVNVPASEVEALIKNNICAKLNFVAKP----ICNDYVDNY 202
T+ S C C DV+ +++ A+E E L+ + +++ KP C + VD+Y
Sbjct: 57 TVKSLPCDICKDVITAAG-NLLKDNATEQEILMY--LERTCDWLPKPNMSASCKEIVDSY 113
Query: 203 VPKFMSEIQSTMT-PQQICNT--------------NHFCMAPSIPKPNVDMKGPVT---- 325
+P + I+ M+ P ++C+ NH S P +DM V
Sbjct: 114 LPVILDMIKGQMSHPGEVCSALNLCESLQKHLAELNHQKQLESNQIPELDMAEVVAPFMA 173
Query: 326 -------------------------CNLCEIAVSEMKSFI-SNTENXXXXXXXXXXXXXX 427
C C V++++ + +N+
Sbjct: 174 NIPFLLYPQDGSHSKPQPKKANGNVCQDCIQLVTDVQEALRTNSTFVEALVDHAKEECDR 233
Query: 428 XXXXXXXXXXXVVDRYGDILMTFIINHMDAKSICKLIHACTSVNVQ-------------- 565
+++Y ++ + ++ HM K IC L C V
Sbjct: 234 LGPGMSDMCKNYINQYSEVAIQMVM-HMQPKEICVLAGFCDEVKEMPMKTLVPAEVVSEN 292
Query: 566 -------LKPIRK-----MKGIECDACRLVVKKFKEYISDPATETEIKNFLKNDFCYIFG 709
++PI+K I C+ C VVK+ + I + TE EI + L +
Sbjct: 293 VIPALGLVEPIKKDPAPAKADIYCEVCEFVVKEVAKLIDNNRTEEEILHALDKVCSKLPT 352
Query: 710 SDKIQCQQIVDEYFNLILNFILAEVDVDKICKFFGLCNA------------KLSGIECEL 853
S QCQ++VD Y IL+ +L E + +C LC++ + G CE+
Sbjct: 353 SLAEQCQEVVDTYGRSILSILLDEASPELVCSMLHLCSSRGLPAATVRVMPRKDGGFCEV 412
Query: 854 CEHVMDEVIKVVTSNHSE 907
C+ ++ + + + N ++
Sbjct: 413 CKKLVGYLDRNLEKNSTK 430
Score = 47.0 bits (110), Expect = 7e-05
Identities = 35/175 (20%), Positives = 62/175 (35%), Gaps = 3/175 (1%)
Frame = +2
Query: 53 CQTCLDVVVMLKKDMVNVPASEVEALIKNNICAKLNFVAKPICNDYVDNYVPKFMSEIQS 232
C+ C VV + K + N E + +C+KL C + VD Y +S +
Sbjct: 316 CEVCEFVVKEVAKLIDNNRTEEEILHALDKVCSKLPTSLAEQCQEVVDTYGRSILSILLD 375
Query: 233 TMTPQQICNTNHFCMAPSIPKPNVDM---KGPVTCNLCEIAVSEMKSFISNTENXXXXXX 403
+P+ +C+ H C + +P V + K C +C+ V + +
Sbjct: 376 EASPELVCSMLHLCSSRGLPAATVRVMPRKDGGFCEVCKKLVGYLDRNLEKNSTKEQILA 435
Query: 404 XXXXXXXXXXXXXXXXXXXVVDRYGDILMTFIINHMDAKSICKLIHACTSVNVQL 568
V Y +L+ ++ MD +C I AC + + L
Sbjct: 436 ALEKGCSFLPDQYRKQCDQFVTEYEPVLIEILVEVMDPSFVCLKIGACPAAHKPL 490
Score = 41.6 bits (96), Expect = 0.003
Identities = 28/102 (27%), Positives = 48/102 (47%), Gaps = 2/102 (1%)
Frame = +2
Query: 527 CKLIHACTSVNVQLKPIRKMKGIECDACRLVVKKFKEYISDPATETEIKNFLKNDFCYIF 706
C + C V KP +K + CD C+ V+ + D ATE EI +L+ ++
Sbjct: 42 CGAVQHCLQT-VWSKPT--VKSLPCDICKDVITAAGNLLKDNATEQEILMYLERTCDWLP 98
Query: 707 GSD-KIQCQQIVDEYFNLILNFILAEVD-VDKICKFFGLCNA 826
+ C++IVD Y +IL+ I ++ ++C LC +
Sbjct: 99 KPNMSASCKEIVDSYLPVILDMIKGQMSHPGEVCSALNLCES 140
>sp|O13035|SAP_CHICK Proactivator polypeptide precursor [Contains: Saposin A; Saposin B;
Saposin C; Saposin D]
Length = 518
Score = 69.7 bits (169), Expect = 1e-11
Identities = 55/283 (19%), Positives = 111/283 (39%), Gaps = 25/283 (8%)
Frame = +2
Query: 53 CQTCLDVVVMLKKDMVNVPASEVEALIKN--NICAKLNFVAKPICNDYVDNYVPKFMSEI 226
CQ C+ +V ++ + V A+ V++L+ + C +L +C Y+ Y + +
Sbjct: 197 CQDCIRLVTDVQ-EAVRTNATFVKSLVAHAKEECDRLGPGMSDMCKSYISEYSDLAIQMM 255
Query: 227 Q--STMTPQQICNTNHFCMA--------------------PSIPKPNVDMKGPVTCNLCE 340
P+ IC FC + ++ K V K C +CE
Sbjct: 256 MHMKDQQPKDICAMVGFCPSVKSVPLQTLVPAQVVHEVKMETVEKATVQEKTFSVCEICE 315
Query: 341 IAVSEMKSFISNTENXXXXXXXXXXXXXXXXXXXXXXXXXVVDRYGDILMTFIINHMDAK 520
V E+ + + + ++ YG L+ ++ + +
Sbjct: 316 TMVKEVTGLLESNKTEEEIVHEMEVVCYLLPASVKDQCKDFIEVYGQALIDMLLEATNPE 375
Query: 521 SICKLIHACTSVNVQLKPIR-KMKGIECDACRLVVKKFKEYISDPATETEIKNFLKNDFC 697
++C ++ C + +P+ K G CD C+++V + + AT TEI+ L+
Sbjct: 376 AVCVMLKCCAANKPPQQPVVVKPAGGFCDICKMIVAYADKELEKNATTTEIEALLEKVCH 435
Query: 698 YIFGSDKIQCQQIVDEYFNLILNFILAEVDVDKICKFFGLCNA 826
++ S QC Q V++Y +++ + +D +C G+C A
Sbjct: 436 FLPESVSDQCVQFVEQYEPVVVQLLAEMMDPTFVCTKLGVCGA 478
Score = 45.8 bits (107), Expect = 2e-04
Identities = 31/105 (29%), Positives = 51/105 (48%), Gaps = 3/105 (2%)
Frame = +2
Query: 521 SICKLIHACTSVNVQLKPIRKMKGIECDACRLVVKKFKEYISDPATETEIKNFLKNDFCY 700
S C + C NV KP + I CD C+ +V + + D TE EI+++L+ C
Sbjct: 41 SQCGAVKHCQQ-NVWSKPA--VNSIPCDLCKELVTVVGKVLKDNGTEDEIRSYLEKR-CE 96
Query: 701 IFGSDKI--QCQQIVDEYFNLILNFILAEVD-VDKICKFFGLCNA 826
+ +C++IVD Y +I++ I E D + +C LC +
Sbjct: 97 FLPDQGLASECKEIVDSYLPVIMDMIKEEFDKPEVVCSALSLCQS 141
Score = 33.9 bits (76), Expect = 0.64
Identities = 21/81 (25%), Positives = 34/81 (41%), Gaps = 1/81 (1%)
Frame = +2
Query: 53 CQTCLDVVVMLKKDMV-NVPASEVEALIKNNICAKLNFVAKPICNDYVDNYVPKFMSEIQ 229
C C +V K++ N +E+EAL++ +C L C +V+ Y P + +
Sbjct: 403 CDICKMIVAYADKELEKNATTTEIEALLEK-VCHFLPESVSDQCVQFVEQYEPVVVQLLA 461
Query: 230 STMTPQQICNTNHFCMAPSIP 292
M P +C C A P
Sbjct: 462 EMMDPTFVCTKLGVCGAAKKP 482
Score = 30.0 bits (66), Expect = 9.3
Identities = 34/149 (22%), Positives = 54/149 (36%), Gaps = 34/149 (22%)
Frame = +2
Query: 563 QLKPIRKMKGIE--CDACRLVVKKFKEYISDPATETEIKNFLKNDFCYIFGSDKIQ-CQQ 733
Q KP +K K E C C +V +E + AT + + C G C+
Sbjct: 183 QDKPKQKSKATEDVCQDCIRLVTDVQEAVRTNATFVKSLVAHAKEECDRLGPGMSDMCKS 242
Query: 734 IVDEYFNLILNFILAEVDVDK--ICKFFGLC------------------NAKLSGIE--- 844
+ EY +L + ++ D IC G C K+ +E
Sbjct: 243 YISEYSDLAIQMMMHMKDQQPKDICAMVGFCPSVKSVPLQTLVPAQVVHEVKMETVEKAT 302
Query: 845 --------CELCEHVMDEVIKVVTSNHSE 907
CE+CE ++ EV ++ SN +E
Sbjct: 303 VQEKTFSVCEICETMVKEVTGLLESNKTE 331
>sp|Q61207|SAP_MOUSE Sulfated glycoprotein 1 precursor (SGP-1) (Prosaposin)
Length = 557
Score = 53.9 bits (128), Expect = 6e-07
Identities = 70/311 (22%), Positives = 120/311 (38%), Gaps = 44/311 (14%)
Frame = +2
Query: 44 SWNCQTCLD-----VVVMLKKDMVNVPASEVEALIKNNICAKLN-FVAKPICNDYVDNYV 205
S +C+ +D ++ M+K +M N P AL N+C L ++A+ N +
Sbjct: 103 SASCKEVVDSYLPVILDMIKGEMSN-PGEVCSAL---NLCQSLQEYLAEQNQKQLESNKI 158
Query: 206 PK---------FMSEIQSTMTPQQICNTNHFCMAPSIPKPNVDMKGPVTCNLCEIAVSEM 358
P+ FMS I + PQ +H P PK N D+ C C VS++
Sbjct: 159 PEVDMARVVAPFMSNIPLLLYPQ-----DHPRSQPQ-PKANEDV-----CQDCMKLVSDV 207
Query: 359 KSFI-SNTENXXXXXXXXXXXXXXXXXXXXXXXXXVVDRYGDILMTFIINHMDA--KSIC 529
++ + +N+ VD+Y ++ + +++ D K IC
Sbjct: 208 QTAVKTNSSFIQGFVDHVKEDCDRLGPGVSDICKNYVDQYSEVCVQMLMHMQDQQPKEIC 267
Query: 530 KLIHACTSVN-VQLKP-------------------------IRKMKGIECDACRLVVKKF 631
L C V V +K ++ I C C+ V+ KF
Sbjct: 268 VLAGFCNEVKRVPMKTLVPATETIKNILPALEMMDPYEQNLVQAHNVILCQTCQFVMNKF 327
Query: 632 KEYISDPATETEIKNFLKNDFCYIFGSDKIQCQQIVDEYFNLILNFILAEVDVDKICKFF 811
E I + ATE + L N + + +CQ++V + +L+ + EV+ +C
Sbjct: 328 SELIVNNATEELLVKGLSNACALLPDPARTKCQEVVGTFGPSLLDIFIHEVNPSSLCGVI 387
Query: 812 GLCNAKLSGIE 844
GLC A+ +E
Sbjct: 388 GLCAARPELVE 398
Score = 50.4 bits (119), Expect = 7e-06
Identities = 61/321 (19%), Positives = 111/321 (34%), Gaps = 65/321 (20%)
Frame = +2
Query: 53 CQTCLDVVVMLKKDMVNVPASEVEALIKN--NICAKLNFVAKPICNDYVDNYVPKFMSEI 226
CQ C+ +V ++ V +S ++ + + C +L IC +YVD Y + +
Sbjct: 197 CQDCMKLVSDVQT-AVKTNSSFIQGFVDHVKEDCDRLGPGVSDICKNYVDQYSEVCVQML 255
Query: 227 Q--STMTPQQICNTNHFC-------MAPSIPK--------PNVDMKGP-----------V 322
P++IC FC M +P P ++M P +
Sbjct: 256 MHMQDQQPKEICVLAGFCNEVKRVPMKTLVPATETIKNILPALEMMDPYEQNLVQAHNVI 315
Query: 323 TCNLCEIAVSEMKSFISNTENXXXXXXXXXXXXXXXXXXXXXXXXXVVDRYGDILMTFII 502
C C+ +++ I N VV +G L+ I
Sbjct: 316 LCQTCQFVMNKFSELIVNNATEELLVKGLSNACALLPDPARTKCQEVVGTFGPSLLDIFI 375
Query: 503 NHMDAKSICKLIHACTS---------------VNVQLK--------------------PI 577
+ ++ S+C +I C + V+ LK P
Sbjct: 376 HEVNPSSLCGVIGLCAARPELVEALEQPAPAIVSALLKEPTPPKQPAQPKQSALPAHVPP 435
Query: 578 RKMKGIECDACRLVVKKFKEYISDPATETEIKNFLKNDFCYIFGSDKIQCQQIVDEYFNL 757
+K G C+ C+ +V + + +T+ EI L+ ++ + QC V EY L
Sbjct: 436 QKNGGF-CEVCKKLVLYLEHNLEKNSTKEEILAALEKGCSFLPDPYQKQCDDFVAEYEPL 494
Query: 758 ILNFILAEVDVDKICKFFGLC 820
+L ++ +D +C G+C
Sbjct: 495 LLEILVEVMDPGFVCSKIGVC 515
Score = 31.6 bits (70), Expect = 3.2
Identities = 20/87 (22%), Positives = 39/87 (44%), Gaps = 1/87 (1%)
Frame = +2
Query: 17 PSNTTVTLNSWNCQTCLDVVVMLKKDMV-NVPASEVEALIKNNICAKLNFVAKPICNDYV 193
P++ N C+ C +V+ L+ ++ N E+ A ++ C+ L + C+D+V
Sbjct: 430 PAHVPPQKNGGFCEVCKKLVLYLEHNLEKNSTKEEILAALEKG-CSFLPDPYQKQCDDFV 488
Query: 194 DNYVPKFMSEIQSTMTPQQICNTNHFC 274
Y P + + M P +C+ C
Sbjct: 489 AEYEPLLLEILVEVMDPGFVCSKIGVC 515
>sp|P50405|PSPB_MOUSE Pulmonary surfactant-associated protein B precursor (SP-B)
(Pulmonary surfactant-associated proteolipid SPL(Phe))
Length = 377
Score = 53.5 bits (127), Expect = 8e-07
Identities = 60/320 (18%), Positives = 112/320 (35%), Gaps = 49/320 (15%)
Frame = +2
Query: 53 CQTCLDVVVMLKKDMVNVPASEVEALIKNNICAKLNF-VAKPICNDYVDNYVPKFMSEIQ 229
CQ C D+V +L K E C L + P C +D Y+P + Q
Sbjct: 68 CQECEDIVHLLTKMTKEDAFQEAIRKFLEQECDILPLKLLVPRCRQVLDVYLPLVIDYFQ 127
Query: 230 STMTPQQICNTNHFC------------MAPSIPKPNVDM-------------KGPVT--- 325
S + P+ ICN C M ++P P +D GP T
Sbjct: 128 SQINPKAICNHVGLCPRGQAKPEQNPGMPDAVPNPLLDKLVLPVLPGALLARPGPHTQDF 187
Query: 326 -----------CNLCEIAVSEMKSFISNTENXXXXXXXXXXXXXXXXXXXXXXXXXVVDR 472
C LC + +++ I + +R
Sbjct: 188 SEQQLPIPLPFCWLCRTLIKRVQAVIPK----GVLAVAVSQVCHVVPLVVGGICQCLAER 243
Query: 473 YGDILMTFIINHMDAKSICKLIHACTSVNVQ------LKPIRK---MKGIECDACRLVVK 625
Y +L+ ++ + + +C L+ C++ + ++P+ + ++ EC C+ V+
Sbjct: 244 YTVLLLDALLGRVVPQLVCGLVLRCSTEDAMGPALPAVEPLIEEWPLQDTECHFCKSVIN 303
Query: 626 KFKEYISDPATETEIKNFLKNDFCYIFGSDKIQCQQIVDEYFNLILNFILAEVDVDKICK 805
+ T + + C F D+ +C+Q V+++ +L + D C+
Sbjct: 304 QAWN------TSEQAMPQAMHQACLRFWLDRQKCEQFVEQHMPQLLALVPRSQDAHITCQ 357
Query: 806 FFGLCNAKLSGIECELCEHV 865
G+C A S ++C H+
Sbjct: 358 ALGVCEAPASPLQCFQTPHL 377
>sp|P10960|SAP_RAT Sulfated glycoprotein 1 precursor (SGP-1) (Prosaposin)
Length = 554
Score = 52.0 bits (123), Expect = 2e-06
Identities = 31/102 (30%), Positives = 52/102 (50%), Gaps = 2/102 (1%)
Frame = +2
Query: 527 CKLIHACTSVNVQLKPIRKMKGIECDACRLVVKKFKEYISDPATETEIKNFLKNDFCYIF 706
C+ + C + V KP K + CD C+ VV + + D ATE EI ++L+ +I
Sbjct: 42 CRAVKHCQQM-VWSKPTAK--SLPCDICKTVVTEAGNLLKDNATEEEILHYLEKTCAWIH 98
Query: 707 GSD-KIQCQQIVDEYFNLILNFILAEV-DVDKICKFFGLCNA 826
S C+++VD Y +IL+ I E+ + ++C LC +
Sbjct: 99 DSSLSASCKEVVDSYLPVILDMIKGEMSNPGEVCSALNLCQS 140
Score = 50.8 bits (120), Expect = 5e-06
Identities = 60/320 (18%), Positives = 115/320 (35%), Gaps = 64/320 (20%)
Frame = +2
Query: 53 CQTCLDVVVMLKKDMVNVPASEVEALIKN--NICAKLNFVAKPICNDYVDNYVPKFMSEI 226
CQ C+ +V ++ V +S V+ L+ + C +L IC +YVD Y + ++
Sbjct: 197 CQDCMKLVTDIQT-AVRTNSSFVQGLVDHVKEDCDRLGPGVSDICKNYVDQY-SEVAVQM 254
Query: 227 QSTMTPQQICNTNHFC-------MAPSIPK--------PNVDMKGP-----------VTC 328
M P++IC FC M +P P +++ P + C
Sbjct: 255 MMHMQPKEICVMVGFCDEVKRVPMRTLVPATEAIKNILPALELTDPYEQDVIQAQNVIFC 314
Query: 329 NLCEIAVSEMKSFISNTENXXXXXXXXXXXXXXXXXXXXXXXXXVVDRYGDILMTFIINH 508
+C++ + ++ I N V+ +G L+ +++
Sbjct: 315 QVCQLVMRKLSELIINNATEELLIKGLSKACSLLPAPASTKCQEVLVTFGPSLLDVLMHE 374
Query: 509 MDAKSICKLIHACTSVNVQLK------------------------------------PIR 580
++ +C +I C S N L P +
Sbjct: 375 VNPNFLCGVISLC-SANPNLVGTLEQPAAAIVSALPKEPAPPKQPEEPKQSALRAHVPPQ 433
Query: 581 KMKGIECDACRLVVKKFKEYISDPATETEIKNFLKNDFCYIFGSDKIQCQQIVDEYFNLI 760
K G C+ C+ +V + + +T+ EI L+ ++ + QC + V EY L+
Sbjct: 434 KNGGF-CEVCKKLVIYLEHNLEKNSTKEEILAALEKGCSFLPDPYQKQCDEFVAEYEPLL 492
Query: 761 LNFILAEVDVDKICKFFGLC 820
L ++ +D +C G+C
Sbjct: 493 LEILVEVMDPSFVCSKIGVC 512
Score = 46.6 bits (109), Expect = 1e-04
Identities = 62/303 (20%), Positives = 115/303 (37%), Gaps = 42/303 (13%)
Frame = +2
Query: 44 SWNCQTCLD-----VVVMLKKDMVNVPASEVEALIKNNICAKLN-FVAKPICNDYVDNYV 205
S +C+ +D ++ M+K +M N P AL N+C L ++A+ N +
Sbjct: 103 SASCKEVVDSYLPVILDMIKGEMSN-PGEVCSAL---NLCQSLQEYLAEQNQRQLESNKI 158
Query: 206 PK---------FMSEIQSTMTPQQICNTNHFCMAPSIPKPNVDMKGPVTCNLCEIAVSEM 358
P+ FMS I + PQ + PK N D+ C C V+++
Sbjct: 159 PEVDLARVVAPFMSNIPLLLYPQDRPRSQ------PQPKANEDV-----CQDCMKLVTDI 207
Query: 359 KSFI-SNTENXXXXXXXXXXXXXXXXXXXXXXXXXVVDRYGDILMTFIINHMDAKSICKL 535
++ + +N+ VD+Y ++ + ++ HM K IC +
Sbjct: 208 QTAVRTNSSFVQGLVDHVKEDCDRLGPGVSDICKNYVDQYSEVAVQMMM-HMQPKEICVM 266
Query: 536 IHACTSVN--------------------------VQLKPIRKMKGIECDACRLVVKKFKE 637
+ C V + I+ I C C+LV++K E
Sbjct: 267 VGFCDEVKRVPMRTLVPATEAIKNILPALELTDPYEQDVIQAQNVIFCQVCQLVMRKLSE 326
Query: 638 YISDPATETEIKNFLKNDFCYIFGSDKIQCQQIVDEYFNLILNFILAEVDVDKICKFFGL 817
I + ATE + L + +CQ+++ + +L+ ++ EV+ + +C L
Sbjct: 327 LIINNATEELLIKGLSKACSLLPAPASTKCQEVLVTFGPSLLDVLMHEVNPNFLCGVISL 386
Query: 818 CNA 826
C+A
Sbjct: 387 CSA 389
Score = 30.4 bits (67), Expect = 7.1
Identities = 18/79 (22%), Positives = 36/79 (45%), Gaps = 1/79 (1%)
Frame = +2
Query: 41 NSWNCQTCLDVVVMLKKDMV-NVPASEVEALIKNNICAKLNFVAKPICNDYVDNYVPKFM 217
N C+ C +V+ L+ ++ N E+ A ++ C+ L + C+++V Y P +
Sbjct: 435 NGGFCEVCKKLVIYLEHNLEKNSTKEEILAALEKG-CSFLPDPYQKQCDEFVAEYEPLLL 493
Query: 218 SEIQSTMTPQQICNTNHFC 274
+ M P +C+ C
Sbjct: 494 EILVEVMDPSFVCSKIGVC 512
>sp|P15285|PSPB_RABIT Pulmonary surfactant-associated protein B precursor (SP-B) (6 kDa
protein) (Pulmonary surfactant-associated proteolipid
SPL(Phe))
Length = 370
Score = 51.2 bits (121), Expect = 4e-06
Identities = 65/311 (20%), Positives = 111/311 (35%), Gaps = 41/311 (13%)
Frame = +2
Query: 53 CQTCLDVVVMLKKDMVNVPASEVEALIKNNICAKLNF-VAKPICNDYVDNYVPKFMSEIQ 229
CQ C D+V +L K + + C L + P C+ +D Y P ++ Q
Sbjct: 70 CQECQDIVNILTKMTKEAIFQDTIRKFLEHECDVLPLKLLVPQCHHVLDVYFPLTITYFQ 129
Query: 230 STMTPQQICNTNHFCMAPSIPKPNVD-----------------MKGPVT----------- 325
S + + IC C P P+P +D GP T
Sbjct: 130 SQINAKAICQHLGLCQ-PGSPEPPLDPLPDKLVLPTLLGALPAKPGPHTQDLSAQRFPIP 188
Query: 326 ---CNLCEIAVSEMKSFISNTENXXXXXXXXXXXXXXXXXXXXXXXXXVVDRYGDILMTF 496
C LC + +++ I + +RY IL+
Sbjct: 189 LPLCWLCRTLLKRIQAMIPK----GVLAMAVAQVCHVVPLVVGGICQCLAERYTVILLEV 244
Query: 497 IINHMDAKSICKLIHACTSVNV--QLKPIRKM-------KGIECDACRLVVKKFKEYISD 649
++ H+ + +C L+ C+SV+ Q+ P + + EC C V + + IS+
Sbjct: 245 LLGHVLPQLVCGLVLRCSSVDSIGQVPPTLEALPGEWLPQDPECRLCMSVTTQARN-ISE 303
Query: 650 PATETEIKNFLKNDFCYIFGSDKIQCQQIVDEYFNLILNFILAEVDVDKICKFFGLCNAK 829
+ + C DK +C+Q V+ + +L+ + D IC+ G C A
Sbjct: 304 QTRPQAVYHA-----CLSSQLDKQECEQFVELHTPQLLSLLSRGWDARAICQALGACVAT 358
Query: 830 LSGIECELCEH 862
LS ++C H
Sbjct: 359 LSPLQCIQSPH 369
>sp|P20097|SAP_CAVPO Saposin C (CO-beta-glucosidase) (Glucosylceramidase activator)
(Sphingolipid activator protein 2) (SAP-2)
Length = 81
Score = 49.3 bits (116), Expect = 1e-05
Identities = 26/78 (33%), Positives = 41/78 (52%)
Frame = +2
Query: 587 KGIECDACRLVVKKFKEYISDPATETEIKNFLKNDFCYIFGSDKIQCQQIVDEYFNLILN 766
+ + C AC VVKK E I + TE +I + L + + S CQ++VD Y + I+
Sbjct: 1 ESVTCKACEYVVKKVMELIDNNRTEEKIIHALDSVCALLPESVSEVCQEVVDTYGDSIVA 60
Query: 767 FILAEVDVDKICKFFGLC 820
+L E+ + +C GLC
Sbjct: 61 LLLQEMSPELVCSELGLC 78
Score = 35.4 bits (80), Expect = 0.22
Identities = 18/78 (23%), Positives = 27/78 (34%)
Frame = +2
Query: 320 VTCNLCEIAVSEMKSFISNTENXXXXXXXXXXXXXXXXXXXXXXXXXVVDRYGDILMTFI 499
VTC CE V ++ I N VVD YGD ++ +
Sbjct: 3 VTCKACEYVVKKVMELIDNNRTEEKIIHALDSVCALLPESVSEVCQEVVDTYGDSIVALL 62
Query: 500 INHMDAKSICKLIHACTS 553
+ M + +C + C S
Sbjct: 63 LQEMSPELVCSELGLCMS 80
Score = 35.0 bits (79), Expect = 0.29
Identities = 19/79 (24%), Positives = 36/79 (45%)
Frame = +2
Query: 44 SWNCQTCLDVVVMLKKDMVNVPASEVEALIKNNICAKLNFVAKPICNDYVDNYVPKFMSE 223
S C+ C VV + + + N E +++CA L +C + VD Y ++
Sbjct: 2 SVTCKACEYVVKKVMELIDNNRTEEKIIHALDSVCALLPESVSEVCQEVVDTYGDSIVAL 61
Query: 224 IQSTMTPQQICNTNHFCMA 280
+ M+P+ +C+ CM+
Sbjct: 62 LLQEMSPELVCSELGLCMS 80
>sp|P22355|PSPB_RAT Pulmonary surfactant-associated protein B precursor (SP-B)
(Pulmonary surfactant-associated proteolipid SPL(Phe))
Length = 376
Score = 48.9 bits (115), Expect = 2e-05
Identities = 56/320 (17%), Positives = 110/320 (34%), Gaps = 49/320 (15%)
Frame = +2
Query: 53 CQTCLDVVVMLKKDMVNVPASEVEALIKNNICAKLNF-VAKPICNDYVDNYVPKFMSEIQ 229
CQ C D+V +L K + C L + P C +D Y+P + Q
Sbjct: 67 CQECEDIVHLLTKMTKEDAFQDTIRKFLEQECDILPLKLLVPRCRQVLDVYLPLVIDYFQ 126
Query: 230 STMTPQQICNTNHFC-------------------------MAPSIPKPNVDMKGPVT--- 325
+ P+ IC+ C + P++P + GP T
Sbjct: 127 GQIKPKAICSHVGLCPLGQTKPEQKPEMLDAIPNPLLNKLVLPALPGAFLARPGPHTQDL 186
Query: 326 -----------CNLCEIAVSEMKSFISNTENXXXXXXXXXXXXXXXXXXXXXXXXXVVDR 472
C LC + +++ I + +R
Sbjct: 187 SEQQLPIPLPFCWLCRTLIKRVQAVIPK----GVLAVAVSQVCHVVPLVVGGICQCLAER 242
Query: 473 YGDILMTFIINHMDAKSICKLIHACTSVNV------QLKPIRK---MKGIECDACRLVVK 625
Y +L+ ++ + + +C L+ C++ + L+P+ + ++ EC C+ V+
Sbjct: 243 YTVLLLDALLGRVVPQLVCGLVLRCSTADAIGPALPALEPLIEKWPLQDTECHFCKSVIN 302
Query: 626 KFKEYISDPATETEIKNFLKNDFCYIFGSDKIQCQQIVDEYFNLILNFILAEVDVDKICK 805
+ T + + C F D+ +C+Q V+++ +L + D C+
Sbjct: 303 QAWN------TSEQAMPQAMHQACLRFWLDRQKCEQFVEQHMPQLLALVPRSQDAHTSCQ 356
Query: 806 FFGLCNAKLSGIECELCEHV 865
G+C A S ++C H+
Sbjct: 357 ALGVCEAPASPLQCFQTPHL 376
>sp|P07988|PSPB_HUMAN Pulmonary surfactant-associated protein B precursor (SP-B) (6 kDa
protein) (Pulmonary surfactant-associated proteolipid
SPL(Phe)) (18 kDa pulmonary-surfactant protein)
Length = 381
Score = 42.4 bits (98), Expect = 0.002
Identities = 19/79 (24%), Positives = 40/79 (50%), Gaps = 2/79 (2%)
Frame = +2
Query: 599 CDACRLVVKKFKEYISDPATETEIKNFLKNDFCYIFGSDKI--QCQQIVDEYFNLILNFI 772
C C +V + + + ++ FL+ + C + + QC Q++D+YF L++++
Sbjct: 69 CQECEDIVHILNKMAKEAIFQDTMRKFLEQE-CNVLPLKLLMPQCNQVLDDYFPLVIDYF 127
Query: 773 LAEVDVDKICKFFGLCNAK 829
+ D + IC GLC ++
Sbjct: 128 QNQTDSNGICMHLGLCKSR 146
Score = 37.0 bits (84), Expect = 0.076
Identities = 59/318 (18%), Positives = 102/318 (32%), Gaps = 53/318 (16%)
Frame = +2
Query: 53 CQTCLDVVVMLKKDMVNVPASEVEALIKNNICAKLNF-VAKPICNDYVDNYVPKFMSEIQ 229
CQ C D+V +L K + C L + P CN +D+Y P + Q
Sbjct: 69 CQECEDIVHILNKMAKEAIFQDTMRKFLEQECNVLPLKLLMPQCNQVLDDYFPLVIDYFQ 128
Query: 230 STMTPQQICNTNHFC------------MAPSIPKPNVD---------------------M 310
+ IC C M+ +PKP D
Sbjct: 129 NQTDSNGICMHLGLCKSRQPEPEQEPGMSDPLPKPLRDPLPDPLLDKLVLPVLPGALQAR 188
Query: 311 KGPVT--------------CNLCEIAVSEMKSFISNTENXXXXXXXXXXXXXXXXXXXXX 448
GP T C LC + +++ I
Sbjct: 189 PGPHTQDLSEQQFPIPLPYCWLCRALIKRIQAMIPK----GALAVAVAQVCRVVPLVAGG 244
Query: 449 XXXXVVDRYGDILMTFIINHMDAKSICKLIHACTSVNVQLKPIRKM-----KGIECDACR 613
+ +RY IL+ ++ M + +C+L+ C S++ P + EC C
Sbjct: 245 ICQCLAERYSVILLDTLLGRMLPQLVCRLVLRC-SMDDSAGPRSPTGEWLPRDSECHLCM 303
Query: 614 LVVKKFKEYISDPATETEIKNFLKNDFCYIFGSDKIQCQQIVDEYFNLILNFILAEVDVD 793
V + + ++E I + C D+ +C+Q V+++ +L + D
Sbjct: 304 SVTTQ-----AGNSSEQAIPQAMLQA-CVGSWLDREKCKQFVEQHTPQLLTLVPRGWDAH 357
Query: 794 KICKFFGLCNAKLSGIEC 847
C+ G+C S ++C
Sbjct: 358 TTCQALGVCGTMSSPLQC 375
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 99,972,060
Number of Sequences: 369166
Number of extensions: 2033694
Number of successful extensions: 4989
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4731
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4967
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 9174518830
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)