Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_008_N19
(329 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q95M18|ENPL_BOVIN Endoplasmin precursor (94 kDa glucose-... 59 3e-09
sp|P14625|ENPL_HUMAN Endoplasmin precursor (94 kDa glucose-... 59 3e-09
sp|P41148|ENPL_CANFA Endoplasmin precursor (94 kDa glucose-... 59 3e-09
sp|P08712|ENPL_MESAU Endoplasmin (94 kDa glucose-regulated ... 59 4e-09
sp|Q29092|ENPL_PIG Endoplasmin precursor (94 kDa glucose-re... 59 4e-09
sp|P08110|ENPL_CHICK Endoplasmin precursor (Heat shock 108 ... 59 4e-09
sp|P08113|ENPL_MOUSE Endoplasmin precursor (Endoplasmic ret... 57 1e-08
sp|O18750|ENPL_RABIT Endoplasmin (94 kDa glucose-regulated ... 56 2e-08
sp|Q08277|HSP82_MAIZE Heat shock protein 82 54 1e-07
sp|O44001|HSP90_EIMTE Heat shock protein 90 53 2e-07
>sp|Q95M18|ENPL_BOVIN Endoplasmin precursor (94 kDa glucose-regulated protein) (GRP94)
Length = 804
Score = 59.3 bits (142), Expect = 3e-09
Identities = 32/84 (38%), Positives = 49/84 (58%)
Frame = +1
Query: 7 DEQARDIALTLYDTANLRSGYSLQASGGFADRIEKILRTSLNIPHDEAVEDDEPIEEKPA 186
D+ D+A+ L++TA LRSGY L + + DRIE++LR SLNI D VE++ EE+P
Sbjct: 707 DKTVSDLAVVLFETATLRSGYLLPDTKAYGDRIERMLRLSLNIDPDAKVEEEP--EEEPE 764
Query: 187 EDEGEVPEPPKTEQPIENKEAVDK 258
E + E + ++ E D+
Sbjct: 765 ETTEDTAEDTEQDEEEEMDAGTDE 788
>sp|P14625|ENPL_HUMAN Endoplasmin precursor (94 kDa glucose-regulated protein) (GRP94)
(gp96 homolog) (Tumor rejection antigen 1)
Length = 803
Score = 58.9 bits (141), Expect = 3e-09
Identities = 33/75 (44%), Positives = 47/75 (62%), Gaps = 1/75 (1%)
Frame = +1
Query: 7 DEQARDIALTLYDTANLRSGYSLQASGGFADRIEKILRTSLNIPHDEAVEDD-EPIEEKP 183
D+ D+A+ L++TA LRSGY L + + DRIE++LR SLNI D VE++ E E+
Sbjct: 707 DKTVLDLAVVLFETATLRSGYLLPDTKAYGDRIERMLRLSLNIDPDAKVEEEPEEEPEET 766
Query: 184 AEDEGEVPEPPKTEQ 228
AED E E + E+
Sbjct: 767 AEDTTEDTEQDEDEE 781
>sp|P41148|ENPL_CANFA Endoplasmin precursor (94 kDa glucose-regulated protein) (GRP94)
Length = 804
Score = 58.9 bits (141), Expect = 3e-09
Identities = 31/83 (37%), Positives = 49/83 (59%)
Frame = +1
Query: 7 DEQARDIALTLYDTANLRSGYSLQASGGFADRIEKILRTSLNIPHDEAVEDDEPIEEKPA 186
D+ D+A+ L++TA LRSGY L + + DRIE++LR SLNI + D +EE+P
Sbjct: 707 DKTVSDLAVVLFETATLRSGYLLPDTKAYGDRIERMLRLSLNI------DPDAKVEEEPE 760
Query: 187 EDEGEVPEPPKTEQPIENKEAVD 255
E+ E E + +++E +D
Sbjct: 761 EEPEETTEDTTEDTEQDDEEEMD 783
>sp|P08712|ENPL_MESAU Endoplasmin (94 kDa glucose-regulated protein) (GRP94)
Length = 400
Score = 58.5 bits (140), Expect = 4e-09
Identities = 30/80 (37%), Positives = 48/80 (60%)
Frame = +1
Query: 7 DEQARDIALTLYDTANLRSGYSLQASGGFADRIEKILRTSLNIPHDEAVEDDEPIEEKPA 186
D+ D+A+ L++TA LRSGY L + +ADRIE++LR SLNI + VE++ E +
Sbjct: 304 DKTVLDLAVVLFETATLRSGYLLPDTKAYADRIERMLRLSLNIDPEAQVEEEPEEEPEDT 363
Query: 187 EDEGEVPEPPKTEQPIENKE 246
++ E E + + E +E
Sbjct: 364 TEDTEQDEEEEVDAGTEEEE 383
>sp|Q29092|ENPL_PIG Endoplasmin precursor (94 kDa glucose-regulated protein) (GRP94)
(GP96 homolog) (98 kDa protein kinase) (PPK 98) (ppk98)
Length = 804
Score = 58.5 bits (140), Expect = 4e-09
Identities = 32/84 (38%), Positives = 48/84 (57%)
Frame = +1
Query: 7 DEQARDIALTLYDTANLRSGYSLQASGGFADRIEKILRTSLNIPHDEAVEDDEPIEEKPA 186
D+ D+A+ L++TA LRSGY L + + DRIE++LR SLNI D VE++ EE+P
Sbjct: 707 DKTVSDLAVVLFETATLRSGYLLPDTKAYGDRIERMLRLSLNIDPDAKVEEEP--EEEPE 764
Query: 187 EDEGEVPEPPKTEQPIENKEAVDK 258
E + E + + E D+
Sbjct: 765 ETTEDTTEDTEQDDDEEMDAGADE 788
>sp|P08110|ENPL_CHICK Endoplasmin precursor (Heat shock 108 kDa protein) (HSP108) (HSP
108) (Transferrin-binding protein)
Length = 795
Score = 58.5 bits (140), Expect = 4e-09
Identities = 32/68 (47%), Positives = 44/68 (64%)
Frame = +1
Query: 7 DEQARDIALTLYDTANLRSGYSLQASGGFADRIEKILRTSLNIPHDEAVEDDEPIEEKPA 186
D+ D+A+ L++TA LRSGY L + + DRIE++LR SLNI D VE +EP E + A
Sbjct: 706 DKTVSDLAVVLFETATLRSGYMLPDTKEYGDRIERMLRLSLNIDLDAKVE-EEPEEPEDA 764
Query: 187 EDEGEVPE 210
+E E E
Sbjct: 765 AEEAEQDE 772
>sp|P08113|ENPL_MOUSE Endoplasmin precursor (Endoplasmic reticulum protein 99) (94 kDa
glucose-regulated protein) (GRP94) (ERP99) (Polymorphic
tumor rejection antigen 1) (Tumor rejection antigen
gp96)
Length = 802
Score = 57.0 bits (136), Expect = 1e-08
Identities = 35/93 (37%), Positives = 52/93 (55%), Gaps = 9/93 (9%)
Frame = +1
Query: 7 DEQARDIALTLYDTANLRSGYSLQASGGFADRIEKILRTSLNIPHDEAVEDDEPIE---- 174
D+ D+A+ L++TA LRSGY L + + DRIE++LR SLNI + VE++ E
Sbjct: 707 DKTVMDLAVVLFETATLRSGYLLPDTKAYGDRIERMLRLSLNIDPEAQVEEEPEEEPEDT 766
Query: 175 ----EKPAEDEGEVPEPPKTEQPIE-NKEAVDK 258
E +DEGE + E+ E KE+ +K
Sbjct: 767 SEDAEDSEQDEGEEMDAGTEEEEEETEKESTEK 799
>sp|O18750|ENPL_RABIT Endoplasmin (94 kDa glucose-regulated protein) (GRP94)
Length = 716
Score = 56.2 bits (134), Expect = 2e-08
Identities = 33/74 (44%), Positives = 47/74 (63%)
Frame = +1
Query: 7 DEQARDIALTLYDTANLRSGYSLQASGGFADRIEKILRTSLNIPHDEAVEDDEPIEEKPA 186
D+ D+A+ L++TA LRSGY L + + DRIE+I+R SLNI D VE++ EE+P
Sbjct: 621 DKTVMDLAVVLFETAILRSGYLLPDTKAYGDRIERIVRLSLNIDPDAKVEEEP--EEEP- 677
Query: 187 EDEGEVPEPPKTEQ 228
ED E E + E+
Sbjct: 678 EDTTEDTEQDEEEE 691
>sp|Q08277|HSP82_MAIZE Heat shock protein 82
Length = 715
Score = 53.5 bits (127), Expect = 1e-07
Identities = 29/74 (39%), Positives = 42/74 (56%)
Frame = +1
Query: 4 SDEQARDIALTLYDTANLRSGYSLQASGGFADRIEKILRTSLNIPHDEAVEDDEPIEEKP 183
+D+ +D+ L L++TA L SG+SL FA RI ++L+ LNI D A ++D + P
Sbjct: 643 NDKSVKDLVLLLFETALLTSGFSLDDPNTFAARIHRMLKLGLNIDEDAAADED---ADMP 699
Query: 184 AEDEGEVPEPPKTE 225
A DEG E E
Sbjct: 700 ALDEGAAEESKMEE 713
>sp|O44001|HSP90_EIMTE Heat shock protein 90
Length = 713
Score = 53.1 bits (126), Expect = 2e-07
Identities = 31/77 (40%), Positives = 45/77 (58%), Gaps = 2/77 (2%)
Frame = +1
Query: 4 SDEQARDIALTLYDTANLRSGYSLQASGGFADRIEKILRTSLNIPHDEAVEDDE--PIEE 177
SD+ +D+ LYDTA L SG+SL+ FA RI ++++ L+I DE +DD+ P+EE
Sbjct: 639 SDKTVKDLIWLLYDTALLTSGFSLEEPTQFAARIHRMIKLGLSIDDDEEAKDDDLPPLEE 698
Query: 178 KPAEDEGEVPEPPKTEQ 228
EG E K E+
Sbjct: 699 V----EGAADEASKMEE 711
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.312 0.134 0.376
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 33,130,045
Number of Sequences: 369166
Number of extensions: 552973
Number of successful extensions: 2853
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2366
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2767
length of database: 68,354,980
effective HSP length: 78
effective length of database: 53,945,650
effective search space used: 1672315150
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)