Planarian EST Database


Dr_sW_008_M23

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_008_M23
         (736 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P05945|MLE_TODPA  Myosin catalytic light chain LC-1, mant...    67   6e-11
sp|P07290|MLE_PATYE  Myosin, essential light chain, adductor...    54   3e-07
sp|P07291|MLE_AEQIR  Myosin essential light chain, striated ...    54   6e-07
sp|P48593|E631_DROME  Calcium-binding protein E63-1                36   0.12 
sp|Q58836|YE41_METJA  Hypothetical protein MJ1441                  31   3.0  
sp|P20664|PRI1_MOUSE  DNA primase small subunit (DNA primase...    31   3.9  
>sp|P05945|MLE_TODPA Myosin catalytic light chain LC-1, mantle muscle
          Length = 160

 Score = 66.6 bits (161), Expect = 6e-11
 Identities = 47/143 (32%), Positives = 70/143 (48%), Gaps = 3/143 (2%)
 Frame = +3

Query: 45  KSEIEDIREVFDLLDFWDGRDGLV*CSQSLENLLRCNGMNPTNRILHQTRCNRKLGFETV 224
           K EIE++REVFDL DFWDGRDG V  ++ + +LLRC GMNPT   +HQ    +K+G +  
Sbjct: 6   KDEIEEVREVFDLFDFWDGRDGDVDAAK-VGDLLRCLGMNPTEAQVHQHGGTKKMGEKAY 64

Query: 225 HILXXXXXXXXXXXXXXXXGNISQNYIGCPQIFLIVMDRV---YFLFQKLRHVLTMYGEK 395
            +                 G  +  ++   + F    DR         ++R+VL M GE+
Sbjct: 65  KL--EEILPIYEEMSSKDTGTAADEFMEAFKTF----DREGQGLISSAEIRNVLKMLGER 118

Query: 396 FVRRRNR*HF*ITDLMKIFDGNV 464
               +    F   D+ +  DGN+
Sbjct: 119 ITEDQCNDIFTFCDIREDIDGNI 141

 Score = 35.4 bits (80), Expect = 0.16
 Identities = 27/90 (30%), Positives = 41/90 (45%)
 Frame = +2

Query: 233 EEFFLIYEKIIQEKGKWKHFAELYWMPSNIFDRDGQGLFPFSEIATRLDYVRREVCQKKK 412
           EE   IYE++  +       A+ +      FDR+GQGL   +EI   L  +   + + + 
Sbjct: 67  EEILPIYEEMSSKDTGTA--ADEFMEAFKTFDREGQGLISSAEIRNVLKMLGERITEDQC 124

Query: 413 QITFLNY*PHEDI*W*REYEEFIKKVFEGP 502
              F      EDI    +YE+ +KKV  GP
Sbjct: 125 NDIFTFCDIREDIDGNIKYEDLMKKVMAGP 154
>sp|P07290|MLE_PATYE Myosin, essential light chain, adductor muscle (Sulfhydryl light
           chain) (SHLC)
          Length = 156

 Score = 54.3 bits (129), Expect = 3e-07
 Identities = 43/143 (30%), Positives = 66/143 (46%), Gaps = 3/143 (2%)
 Frame = +3

Query: 45  KSEIEDIREVFDLLDFWDGRDGLV*CSQSLENLLRCNGMNPTNRILHQTRCNRKLGFETV 224
           + EI+D+++VF+L DFWDGRDG V  +  L ++ RC G+NP N  +       K+G ++ 
Sbjct: 5   QDEIDDLKDVFELFDFWDGRDGAV-DAFKLGDVCRCLGINPRNDDVFAVGGTHKMGEKS- 62

Query: 225 HILXXXXXXXXXXXXXXXXGNISQNYIGCPQIFLIVMDRV---YFLFQKLRHVLTMYGEK 395
             L                     +Y+   + F    DR    +    +LRHVLT  GE+
Sbjct: 63  --LPFEEFLPAYEGLMDCEQGTFADYMEAFKTF----DREGQGFISGAELRHVLTALGER 116

Query: 396 FVRRRNR*HF*ITDLMKIFDGNV 464
                      +TDL +  +GNV
Sbjct: 117 LTDEEIDEIISLTDLQEDLEGNV 139

 Score = 38.1 bits (87), Expect = 0.024
 Identities = 27/92 (29%), Positives = 44/92 (47%), Gaps = 1/92 (1%)
 Frame = +2

Query: 230 FEEFFLIYEKIIQ-EKGKWKHFAELYWMPSNIFDRDGQGLFPFSEIATRLDYVRREVCQK 406
           FEEF   YE ++  E+G +  + E +      FDR+GQG    +E+   L  +   +  +
Sbjct: 65  FEEFLPAYEGLMDCEQGTFADYMEAF----KTFDREGQGFISGAELRHVLTALGERLTDE 120

Query: 407 KKQITFLNY*PHEDI*W*REYEEFIKKVFEGP 502
           +           ED+    +YE+F+KKV  GP
Sbjct: 121 EIDEIISLTDLQEDLEGNVKYEDFVKKVMAGP 152
>sp|P07291|MLE_AEQIR Myosin essential light chain, striated adductor muscle (E-LC)
           (Sulfhydryl light chain) (SHLC)
          Length = 157

 Score = 53.5 bits (127), Expect = 6e-07
 Identities = 43/143 (30%), Positives = 66/143 (46%), Gaps = 3/143 (2%)
 Frame = +3

Query: 45  KSEIEDIREVFDLLDFWDGRDGLV*CSQSLENLLRCNGMNPTNRILHQTRCNRKLGFETV 224
           + EI+D+++VF+L DFWDGRDG V  +  L ++ RC G+NP N  +       K+G ++ 
Sbjct: 6   QDEIDDLKDVFELFDFWDGRDGAV-DAFKLGDVCRCLGINPRNEDVFAVGGTHKMGEKS- 63

Query: 225 HILXXXXXXXXXXXXXXXXGNISQNYIGCPQIFLIVMDRV---YFLFQKLRHVLTMYGEK 395
             L                     +Y+   + F    DR    +    +LRHVLT  GE+
Sbjct: 64  --LPFEEFLPAYEGLMDCEQGTFADYMEAFKTF----DREGQGFISGAELRHVLTALGER 117

Query: 396 FVRRRNR*HF*ITDLMKIFDGNV 464
                      +TDL +  +GNV
Sbjct: 118 LSDEDVDEIIKLTDLQEDLEGNV 140

 Score = 37.7 bits (86), Expect = 0.032
 Identities = 27/92 (29%), Positives = 43/92 (46%), Gaps = 1/92 (1%)
 Frame = +2

Query: 230 FEEFFLIYEKIIQ-EKGKWKHFAELYWMPSNIFDRDGQGLFPFSEIATRLDYVRREVCQK 406
           FEEF   YE ++  E+G +  + E +      FDR+GQG    +E+   L  +   +  +
Sbjct: 66  FEEFLPAYEGLMDCEQGTFADYMEAF----KTFDREGQGFISGAELRHVLTALGERLSDE 121

Query: 407 KKQITFLNY*PHEDI*W*REYEEFIKKVFEGP 502
                       ED+    +YE+F+KKV  GP
Sbjct: 122 DVDEIIKLTDLQEDLEGNVKYEDFVKKVMAGP 153
>sp|P48593|E631_DROME Calcium-binding protein E63-1
          Length = 193

 Score = 35.8 bits (81), Expect = 0.12
 Identities = 20/50 (40%), Positives = 32/50 (64%)
 Frame = +3

Query: 36  PFPKSEIEDIREVFDLLDFWDGRDGLV*CSQSLENLLRCNGMNPTNRILH 185
           PF + EI+D+R  FDLLD    RDG V  +  L+ +L+  G+N ++ ++H
Sbjct: 31  PFTEVEIKDLRTAFDLLD--RNRDGRV-TANELQFMLKNLGINVSDELIH 77
>sp|Q58836|YE41_METJA Hypothetical protein MJ1441
          Length = 1226

 Score = 31.2 bits (69), Expect = 3.0
 Identities = 15/38 (39%), Positives = 24/38 (63%), Gaps = 4/38 (10%)
 Frame = +2

Query: 245 LIYEKII----QEKGKWKHFAELYWMPSNIFDRDGQGL 346
           L+ EK+I    +E+G++     LYWM S+I   DG+G+
Sbjct: 829 LLAEKLINRYLEEEGRYPENIALYWMASDIMWADGEGM 866
>sp|P20664|PRI1_MOUSE DNA primase small subunit (DNA primase 49 kDa subunit) (p49)
          Length = 417

 Score = 30.8 bits (68), Expect = 3.9
 Identities = 16/55 (29%), Positives = 27/55 (49%)
 Frame = +2

Query: 227 YFEEFFLIYEKIIQEKGKWKHFAELYWMPSNIFDRDGQGLFPFSEIATRLDYVRR 391
           YFEE+ L+ + I++ K  W     L  +P  I D   +G   F     R +++R+
Sbjct: 222 YFEEYALVGQDILENKENWDKILAL--VPETIHDELQRGFQKFHSSPQRWEHLRK 274
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 85,357,851
Number of Sequences: 369166
Number of extensions: 1738406
Number of successful extensions: 4331
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 4098
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4325
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 6582889600
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)