Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_008_L04-2
(381 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q5E951|TBCB_BOVIN Tubulin-specific chaperone B (Tubulin ... 80 1e-15
sp|Q9D1E6|TBCB_MOUSE Tubulin-specific chaperone B (Tubulin ... 80 2e-15
sp|Q99426|TBCB_HUMAN Tubulin-specific chaperone B (Tubulin ... 76 3e-14
sp|Q20728|YXHK_CAEEL Hypothetical protein F53F4.3 in chromo... 76 3e-14
sp|P53904|TBCB_YEAST Tubulin-specific chaperone B (Tubulin ... 31 0.77
sp|Q8NH93|OR1L3_HUMAN Olfactory receptor 1L3 (Olfactory rec... 29 2.9
sp|Q9RC99|RECF_BACHD DNA replication and repair protein recF 29 2.9
sp|Q9M568|PAL1_RUBID Phenylalanine ammonia-lyase 1 (RiPAL1) 29 3.8
sp|Q9VWR2|CP308_DROME Probable cytochrome P450 308a1 (CYPCC... 28 5.0
sp|O60721|NCKX1_HUMAN Sodium/potassium/calcium exchanger 1 ... 28 6.5
>sp|Q5E951|TBCB_BOVIN Tubulin-specific chaperone B (Tubulin folding cofactor B)
(Cytoskeleton associated protein 1)
(Cytoskeleton-associated protein CKAPI)
Length = 244
Score = 80.5 bits (197), Expect = 1e-15
Identities = 42/112 (37%), Positives = 70/112 (62%), Gaps = 1/112 (0%)
Frame = +1
Query: 43 ISLFITNNATSVQCEKRYPCNLTVGEFKAKLELVTGCDHRHVKLQVFTEDDEPFCWLDDN 222
+++FI+++ S + +KRY +LTV EFK KL+LV G ++L+++ DD+ C LD +
Sbjct: 11 VNVFISSSLNSFRSQKRYSRSLTVAEFKCKLQLVVGSPASCMELELYGPDDKFCCKLDQD 70
Query: 223 MKALGDYPIKDKMRVHATD-PNLKGGEFETLDESQPLYEISFEEYAKREDTV 375
LG YP+ D R+H D + GE+E + + + YEIS E Y +R+D++
Sbjct: 71 DALLGSYPVDDGCRIHVIDHSGARLGEYEDISKVEK-YEISQEAYEQRQDSI 121
>sp|Q9D1E6|TBCB_MOUSE Tubulin-specific chaperone B (Tubulin folding cofactor B)
(Cytoskeleton associated protein 1)
(Cytoskeleton-associated protein CKAPI)
Length = 244
Score = 79.7 bits (195), Expect = 2e-15
Identities = 43/112 (38%), Positives = 69/112 (61%), Gaps = 1/112 (0%)
Frame = +1
Query: 43 ISLFITNNATSVQCEKRYPCNLTVGEFKAKLELVTGCDHRHVKLQVFTEDDEPFCWLDDN 222
+ +FI+++ S + EKRY +LT+ EFK KLELV G ++L+++ DD+ + LD
Sbjct: 11 VMVFISSSLNSFRSEKRYSRSLTIAEFKCKLELVVGSPASCMELELYGADDKFYSKLDQE 70
Query: 223 MKALGDYPIKDKMRVHATD-PNLKGGEFETLDESQPLYEISFEEYAKREDTV 375
LG YP+ D R+H D ++ GE+E + + + YEIS E Y +R++TV
Sbjct: 71 DALLGSYPVDDGCRIHVIDHSGVRLGEYEDVSKVEK-YEISPEAYERRQNTV 121
>sp|Q99426|TBCB_HUMAN Tubulin-specific chaperone B (Tubulin folding cofactor B)
(Cytoskeleton associated protein 1)
(Cytoskeleton-associated protein CKAPI)
Length = 244
Score = 75.9 bits (185), Expect = 3e-14
Identities = 41/112 (36%), Positives = 67/112 (59%), Gaps = 1/112 (0%)
Frame = +1
Query: 43 ISLFITNNATSVQCEKRYPCNLTVGEFKAKLELVTGCDHRHVKLQVFTEDDEPFCWLDDN 222
+++FI+++ + + EKRY +LT+ EFK KLEL+ G ++L+++ DD+ + LD
Sbjct: 11 VTVFISSSLNTFRSEKRYSRSLTIAEFKCKLELLVGSPASCMELELYGVDDKFYSKLDQE 70
Query: 223 MKALGDYPIKDKMRVHATD-PNLKGGEFETLDESQPLYEISFEEYAKREDTV 375
LG YP+ D R+H D + GE+E + + Y IS E Y +R+DTV
Sbjct: 71 DALLGSYPVDDGCRIHVIDHSGARLGEYEDVSRVEK-YTISQEAYDQRQDTV 121
>sp|Q20728|YXHK_CAEEL Hypothetical protein F53F4.3 in chromosome V
Length = 229
Score = 75.9 bits (185), Expect = 3e-14
Identities = 45/112 (40%), Positives = 64/112 (57%), Gaps = 1/112 (0%)
Frame = +1
Query: 49 LFITNNATSVQCEKRYPCNLTVGEFKAKLELVTGCDHRHVKLQVFTEDDEPFCWLDDNMK 228
L IT NAT EK+YP +++ + K KLELV G +++Q+F DD+ L D K
Sbjct: 7 LEITTNATDFPMEKKYPAGMSLNDLKKKLELVVGTTVDSMRIQLFDGDDQLKGELTDGAK 66
Query: 229 ALGDYPIKDKMRVHATDPNLKGGEFETLDESQ-PLYEISFEEYAKREDTVLA 381
+L D ++D R+HA D + GG + DES YE+S + Y KR D+V A
Sbjct: 67 SLKDLGVRDGYRIHAVD--VTGGNEDFKDESMVEKYEMSDDTYGKRTDSVRA 116
>sp|P53904|TBCB_YEAST Tubulin-specific chaperone B (Tubulin folding cofactor B)
(Alpha-tubulin formation protein 1)
Length = 254
Score = 31.2 bits (69), Expect = 0.77
Identities = 32/114 (28%), Positives = 53/114 (46%), Gaps = 13/114 (11%)
Frame = +1
Query: 76 VQCEKRYPCNLTVGEFKAKLELVTGCDHRHVKLQVFTEDDEPFCWLDDNMKALGDYPIKD 255
V+ EK P +L + +FK +L VTG + +++ V + D + + K G Y +D
Sbjct: 11 VRTEKELPNSLKLRQFKDRLYHVTGVEPEDMEIVVKRQYDNKEIY---STKKGGAYSNED 67
Query: 256 ----------KMRVHATDPNLK--GGEFETLDESQPLYE-ISFEEYAKREDTVL 378
++ V TD N + + T E P E IS E+Y +R+ +VL
Sbjct: 68 EDANFLKGEEELIVVVTDSNAQSISNQLATQAEGIPSMEVISEEDYLRRDQSVL 121
>sp|Q8NH93|OR1L3_HUMAN Olfactory receptor 1L3 (Olfactory receptor 9-D) (OR9-D)
Length = 324
Score = 29.3 bits (64), Expect = 2.9
Identities = 16/46 (34%), Positives = 22/46 (47%)
Frame = -1
Query: 141 QF*FSFEFPNS*VTRISLFALN*CCIVSNKQTYVTTMNNRCHLFVL 4
Q F F N ++ A+N C + N YVT MN RC + +L
Sbjct: 100 QMYFFLVFGNIDSYLLAAMAINRCVAICNPFHYVTVMNRRCCVLLL 145
>sp|Q9RC99|RECF_BACHD DNA replication and repair protein recF
Length = 371
Score = 29.3 bits (64), Expect = 2.9
Identities = 19/65 (29%), Positives = 32/65 (49%), Gaps = 7/65 (10%)
Frame = +1
Query: 166 VKLQVFTEDDEPFCWLDDNMKALGDY-------PIKDKMRVHATDPNLKGGEFETLDESQ 324
++L T D P LDD + L DY I+ +++ T N+ G + +TL E+
Sbjct: 299 IELMKETVGDYPILLLDDVLSELDDYRQSHLLHAIQHRVQTFVTTTNVDGIDHQTLQEA- 357
Query: 325 PLYEI 339
+YE+
Sbjct: 358 TIYEV 362
>sp|Q9M568|PAL1_RUBID Phenylalanine ammonia-lyase 1 (RiPAL1)
Length = 710
Score = 28.9 bits (63), Expect = 3.8
Identities = 18/54 (33%), Positives = 27/54 (50%), Gaps = 1/54 (1%)
Frame = +1
Query: 202 FCWLDDNMKALGDYPIKDKMRVHATDPNLKGGEFETLDESQPLYEI-SFEEYAK 360
F ++DD L YP+ K+R + LK GE E + ++I +FEE K
Sbjct: 570 FSYIDD--PCLATYPLMQKLRAELVEHALKNGERERSANTSIFHKIAAFEEELK 621
>sp|Q9VWR2|CP308_DROME Probable cytochrome P450 308a1 (CYPCCCVIIIA1)
Length = 490
Score = 28.5 bits (62), Expect = 5.0
Identities = 17/52 (32%), Positives = 29/52 (55%)
Frame = +1
Query: 208 WLDDNMKALGDYPIKDKMRVHATDPNLKGGEFETLDESQPLYEISFEEYAKR 363
WL ++ + LG K+++ HAT L+G E + + LYE++ E A+R
Sbjct: 264 WLAESKRGLG----KEELAGHATTLLLEGYETSAMLLAFALYELALNEDAQR 311
>sp|O60721|NCKX1_HUMAN Sodium/potassium/calcium exchanger 1 (Na(+)/K(+)/Ca(2+)-exchange
protein 1) (Retinal rod Na-Ca+K exchanger)
Length = 1099
Score = 28.1 bits (61), Expect = 6.5
Identities = 18/55 (32%), Positives = 30/55 (54%)
Frame = +1
Query: 205 CWLDDNMKALGDYPIKDKMRVHATDPNLKGGEFETLDESQPLYEISFEEYAKRED 369
C ++ + GD +D+ +HA D +KG E ET ESQ E+S E + + ++
Sbjct: 794 CEDENEAEGKGDNEGEDEGEIHAEDGEMKGNEGET--ESQ---ELSAENHGEAKN 843
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 40,037,504
Number of Sequences: 369166
Number of extensions: 713526
Number of successful extensions: 1567
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1553
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1565
length of database: 68,354,980
effective HSP length: 93
effective length of database: 51,174,625
effective search space used: 1688762625
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)