Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_008_L01
(465 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q8BJS4|UN84B_MOUSE Sad1/unc-84-like protein 2 104 1e-22
sp|Q9UH99|UN84B_HUMAN Sad1/unc-84-like protein 2 (Rab5-inte... 102 6e-22
sp|Q9D666|UN84A_MOUSE Sad1/unc-84 protein-like 1 (Unc-84 ho... 95 7e-20
sp|O94901|UN84A_HUMAN Sad1/unc-84 protein-like 1 (Unc-84 ho... 92 6e-19
sp|Q9DA32|SPA4L_MOUSE Sperm-associated antigen 4-like protein 83 3e-16
sp|Q8TC36|SPA4L_HUMAN Sperm-associated antigen 4-like prote... 81 1e-15
sp|Q9NPE6|SPA4_HUMAN Sperm-associated antigen 4 protein (Ou... 79 7e-15
sp|O55034|SPAG4_RAT Sperm-associated antigen 4 protein (Out... 78 9e-15
sp|Q09825|SAD1_SCHPO Spindle pole body associated protein sad1 76 4e-14
sp|Q9JJF2|SPAG4_MOUSE Sperm-associated antigen 4 protein (O... 75 6e-14
>sp|Q8BJS4|UN84B_MOUSE Sad1/unc-84-like protein 2
Length = 699
Score = 104 bits (259), Expect = 1e-22
Identities = 48/116 (41%), Positives = 74/116 (63%), Gaps = 2/116 (1%)
Frame = +1
Query: 19 PGDCWPFSGSIGHAVIQLSHKVLVNAVSLEHLAARLNHDGKILSAPKQFQIYSLSENTYE 198
PG+CW F G G AV++LS ++ AV+LEH+ L+ + I SAPK F I+ E+ +
Sbjct: 580 PGNCWAFQGPQGFAVVRLSARIRPTAVTLEHVPKALSPNSTISSAPKDFAIFGFDEDLQQ 639
Query: 199 KMELIGTFEYKETDQAIQTFLVEVPKIA--RIIRMDVLNNHGNKDYTCIYRLRIHG 360
+ L+GTF Y + + IQTF + K+A +++ + +L N G+ +YTCIYR R+HG
Sbjct: 640 EGTLLGTFAYDQDGEPIQTFYFQASKMATYQVVELRILTNWGHPEYTCIYRFRVHG 695
>sp|Q9UH99|UN84B_HUMAN Sad1/unc-84-like protein 2 (Rab5-interacting protein) (Rab5IP)
Length = 717
Score = 102 bits (253), Expect = 6e-22
Identities = 47/116 (40%), Positives = 73/116 (62%), Gaps = 2/116 (1%)
Frame = +1
Query: 19 PGDCWPFSGSIGHAVIQLSHKVLVNAVSLEHLAARLNHDGKILSAPKQFQIYSLSENTYE 198
PG+CW F G G AV++LS ++ AV+LEH+ L+ + I SAPK F I+ E+ +
Sbjct: 598 PGNCWAFQGPQGFAVVRLSARIRPTAVTLEHVPKALSPNSTISSAPKDFAIFGFDEDLQQ 657
Query: 199 KMELIGTFEYKETDQAIQTFLVEVPKIA--RIIRMDVLNNHGNKDYTCIYRLRIHG 360
+ L+G F Y + + IQTF + P +A +++ + +L N G+ +YTCIYR R+HG
Sbjct: 658 EGTLLGKFTYDQDGEPIQTFHFQAPTMATYQVVELRILTNWGHPEYTCIYRFRVHG 713
>sp|Q9D666|UN84A_MOUSE Sad1/unc-84 protein-like 1 (Unc-84 homolog A)
Length = 913
Score = 95.1 bits (235), Expect = 7e-20
Identities = 46/116 (39%), Positives = 71/116 (61%), Gaps = 2/116 (1%)
Frame = +1
Query: 19 PGDCWPFSGSIGHAVIQLSHKVLVNAVSLEHLAARLNHDGKILSAPKQFQIYSLSENTYE 198
PG+CW F GS G+ V++LS K+ ++EH+ L+ G I SAPK F +Y L E
Sbjct: 794 PGNCWAFKGSQGYLVVRLSMKIYPTTFTMEHIPKTLSPTGNISSAPKDFAVYGLETEYQE 853
Query: 199 KMELIGTFEYKETDQAIQTF-LVEVPKIA-RIIRMDVLNNHGNKDYTCIYRLRIHG 360
+ + +G F Y + ++Q F +E P A +I+ + VL+N G+ +YTC+YR R+HG
Sbjct: 854 EGQPLGRFTYDQEGDSLQMFHTLERPDQAFQIVELRVLSNWGHPEYTCLYRFRVHG 909
>sp|O94901|UN84A_HUMAN Sad1/unc-84 protein-like 1 (Unc-84 homolog A)
Length = 812
Score = 92.0 bits (227), Expect = 6e-19
Identities = 44/117 (37%), Positives = 71/117 (60%), Gaps = 3/117 (2%)
Frame = +1
Query: 19 PGDCWPFSGSIGHAVIQLSHKVLVNAVSLEHLAARLNHDGKILSAPKQFQIYSLSENTYE 198
PG+CW F GS G+ V++LS + A +LEH+ L+ G I SAPK F +Y L E
Sbjct: 692 PGNCWAFKGSQGYLVVRLSMMIHPAAFTLEHIPKTLSPTGNISSAPKDFAVYGLENEYQE 751
Query: 199 KMELIGTFEYKETDQAIQTF-LVEVP--KIARIIRMDVLNNHGNKDYTCIYRLRIHG 360
+ +L+G F Y + +++Q F ++ P +I+ + + +N G+ +YTC+YR R+HG
Sbjct: 752 EGQLLGQFTYDQDGESLQMFQALKRPDDTAFQIVELRIFSNWGHPEYTCLYRFRVHG 808
>sp|Q9DA32|SPA4L_MOUSE Sperm-associated antigen 4-like protein
Length = 348
Score = 82.8 bits (203), Expect = 3e-16
Identities = 39/118 (33%), Positives = 73/118 (61%), Gaps = 2/118 (1%)
Frame = +1
Query: 19 PGDCWPFSGSIGHAVIQLSHKVLVNAVSLEHLAARLNHDGKILSAPKQFQIYSLSENTYE 198
PG+CW F+ G I+L+ KV ++ ++L+H+ ++ G + +APK F IY L E
Sbjct: 221 PGNCWAFASDRGQVTIRLAQKVYLSNITLQHIPKTISLSGSLDTAPKDFVIYGLESLpre 280
Query: 199 KMELIGTFEYKETDQAIQTFLVE--VPKIARIIRMDVLNNHGNKDYTCIYRLRIHGSL 366
++ +G F++ + + IQTF ++ P+ +++ + +N GN +TC+YR+R+HGS+
Sbjct: 281 EV-FLGAFQF-QPENIIQTFQLQNLPPRSFAAVKVKISSNWGNPRFTCMYRVRVHGSV 336
>sp|Q8TC36|SPA4L_HUMAN Sperm-associated antigen 4-like protein (Testis and spermatogenesis
related gene 4 protein)
Length = 379
Score = 80.9 bits (198), Expect = 1e-15
Identities = 38/118 (32%), Positives = 72/118 (61%), Gaps = 2/118 (1%)
Frame = +1
Query: 19 PGDCWPFSGSIGHAVIQLSHKVLVNAVSLEHLAARLNHDGKILSAPKQFQIYSLSENTYE 198
PG+CW F G G IQL+ KV ++ ++L+H+ ++ G + +APK F IY + + E
Sbjct: 248 PGNCWAFEGDRGQVTIQLAQKVYLSNLTLQHIPKTISLSGSLDTAPKDFVIYGMEGSPKE 307
Query: 199 KMELIGTFEYKETDQAIQTFLV--EVPKIARIIRMDVLNNHGNKDYTCIYRLRIHGSL 366
++ +G F++ + + IQ F + + + +++ + +N GN +TC+YR+R+HGS+
Sbjct: 308 EV-FLGAFQF-QPENIIQMFPLQNQPARAFSAVKVKISSNWGNPGFTCLYRVRVHGSV 363
>sp|Q9NPE6|SPA4_HUMAN Sperm-associated antigen 4 protein (Outer dense fiber-associated
protein SPAG4)
Length = 437
Score = 78.6 bits (192), Expect = 7e-15
Identities = 40/117 (34%), Positives = 67/117 (57%), Gaps = 3/117 (2%)
Frame = +1
Query: 19 PGDCWPFSGSIGHAVIQLSHKVLVNAVSLEHLAARLNHDGKILSAPKQFQIYSLSENTYE 198
PG+CW F G G VIQL +V ++ ++L+H + H G SAP+ F ++ L Y+
Sbjct: 308 PGNCWAFEGDQGQVVIQLPGRVQLSDITLQHPPPSVEHTGGANSAPRDFAVFGL--QVYD 365
Query: 199 KMEL-IGTFEYKETDQAIQTFLV--EVPKIARIIRMDVLNNHGNKDYTCIYRLRIHG 360
+ E+ +G F + IQTF + + P +++ +L+N G+ +TC+YR+R HG
Sbjct: 366 ETEVSLGKFTFDVEKSEIQTFHLQNDPPAAFPKVKIQILSNWGHPRFTCLYRVRAHG 422
>sp|O55034|SPAG4_RAT Sperm-associated antigen 4 protein (Outer dense fiber-associated
protein SPAG4)
Length = 444
Score = 78.2 bits (191), Expect = 9e-15
Identities = 38/116 (32%), Positives = 64/116 (55%), Gaps = 2/116 (1%)
Frame = +1
Query: 19 PGDCWPFSGSIGHAVIQLSHKVLVNAVSLEHLAARLNHDGKILSAPKQFQIYSLSENTYE 198
PG+CW F G G VI+L V ++ ++L+H + H G SAP+ F ++ L + E
Sbjct: 310 PGNCWAFEGEQGQVVIRLPGHVQLSDITLQHPPPTVAHTGGASSAPRDFAVFGLQADDDE 369
Query: 199 KMELIGTFEYKETDQAIQTFLV--EVPKIARIIRMDVLNNHGNKDYTCIYRLRIHG 360
+G F ++ IQTF + + P +++ +L+N G+ +TC+YR+R HG
Sbjct: 370 TEVFLGKFIFEVQKSEIQTFHLQNDPPSAFPKVKIQILSNWGHPRFTCLYRVRAHG 425
>sp|Q09825|SAD1_SCHPO Spindle pole body associated protein sad1
Length = 514
Score = 75.9 bits (185), Expect = 4e-14
Identities = 41/118 (34%), Positives = 69/118 (58%), Gaps = 5/118 (4%)
Frame = +1
Query: 28 CWPFSGSIGHAVIQLSHKVLVNAVSLEHLAARLNHDGKILSAPKQFQIYSLSENTYEKME 207
CW F GS G I LS V V V++EH+ ++ HD + SAPK F+++ + + +
Sbjct: 373 CWSFQGSEGQLGISLSRPVYVTNVTIEHVQHKIAHD--LSSAPKDFELW-VQGMSSKMFV 429
Query: 208 LIGTFEYKETDQAIQTFLVE-----VPKIARIIRMDVLNNHGNKDYTCIYRLRIHGSL 366
L+G Y T+ +IQTF E V + + + + + +N GN +YTC+Y++R+HG++
Sbjct: 430 LLGKARYSLTEDSIQTFSFESSNYIVAEPIQNVILKIKSNWGNPNYTCLYQVRVHGTV 487
>sp|Q9JJF2|SPAG4_MOUSE Sperm-associated antigen 4 protein (Outer dense fiber-associated
protein SPAG4)
Length = 443
Score = 75.5 bits (184), Expect = 6e-14
Identities = 39/116 (33%), Positives = 63/116 (54%), Gaps = 2/116 (1%)
Frame = +1
Query: 19 PGDCWPFSGSIGHAVIQLSHKVLVNAVSLEHLAARLNHDGKILSAPKQFQIYSLSENTYE 198
PG+CW F G G VI+L V ++ ++L+H + H G SAP+ F +Y L + E
Sbjct: 310 PGNCWAFEGDKGQVVIRLPGHVQLSDITLQHPPPTVAHTGGASSAPRDFAVYGLQADD-E 368
Query: 199 KMELIGTFEYKETDQAIQTFLV--EVPKIARIIRMDVLNNHGNKDYTCIYRLRIHG 360
+G F + IQTF + + P +++ +L+N G+ +TC+YR+R HG
Sbjct: 369 TEVFLGKFIFDVQKSEIQTFHLQNDPPSAFPKVKIQILSNWGHPRFTCLYRVRAHG 424
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 52,483,989
Number of Sequences: 369166
Number of extensions: 942920
Number of successful extensions: 2376
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2333
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2362
length of database: 68,354,980
effective HSP length: 101
effective length of database: 49,696,745
effective search space used: 2633927485
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)