Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_008_K20
(405 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q890I8|GLGA_LACPL Glycogen synthase (Starch [bacterial g... 32 0.48
sp|Q04704|V6B_CVCAE Protein 6B 30 2.4
sp|O28029|SYA_ARCFU Alanyl-tRNA synthetase (Alanine--tRNA l... 30 2.4
sp|P77206|YAFZ_ECOLI Hypothetical protein yafZ 28 5.3
sp|P41815|BAP3_YEAST Valine amino-acid permease (Branched-c... 28 7.0
sp|P52132|YFJQ_ECOLI Hypothetical protein yfjQ 28 7.0
sp|P19828|ANGR_VIBAN Protein angR 28 9.1
sp|Q46266|PFL_CLOPA Formate acetyltransferase (Pyruvate for... 28 9.1
>sp|Q890I8|GLGA_LACPL Glycogen synthase (Starch [bacterial glycogen] synthase)
Length = 479
Score = 32.0 bits (71), Expect = 0.48
Identities = 13/43 (30%), Positives = 20/43 (46%)
Frame = +3
Query: 24 GENCAYCGYKGVAYSHYEYQIFVETEIKNNTKLRWKWTDGKKF 152
GE+ YCG K + +H +Y + + L W DG +F
Sbjct: 68 GEHAMYCGIKTLTVAHVQYYLIDNLDYFGREGLYGYWDDGARF 110
>sp|Q04704|V6B_CVCAE Protein 6B
Length = 213
Score = 29.6 bits (65), Expect = 2.4
Identities = 26/106 (24%), Positives = 46/106 (43%), Gaps = 9/106 (8%)
Frame = +3
Query: 18 IEGENCAYCGYKGVAYSHYEYQIFVETEIKNNT-KLRWKWTDGKKFPENAIMGGHDGDKM 194
IEG C++ GYK AY+H +Y + + + K D ++ ++G DK
Sbjct: 70 IEGFPCSWPGYKAYAYNHIDYHFDLSSPFYSFVDKFYISLGDREEKIHLRVVGATPKDKR 129
Query: 195 LFIG-RACVENQILVGS---YRRDTGEV----HIPFKGKEYIVKCC 308
L +G R + +G+ + +D + H+ + Y VK C
Sbjct: 130 LTVGCRTSFSVNLPIGTQIYHDKDMQHLVEGRHLECAHRVYFVKYC 175
>sp|O28029|SYA_ARCFU Alanyl-tRNA synthetase (Alanine--tRNA ligase) (AlaRS)
Length = 906
Score = 29.6 bits (65), Expect = 2.4
Identities = 17/68 (25%), Positives = 31/68 (45%), Gaps = 4/68 (5%)
Frame = +3
Query: 75 EYQIFVETEIKNNTKLRWKWTD----GKKFPENAIMGGHDGDKMLFIGRACVENQILVGS 242
E ++ EI N ++W+W D +KF GG + + + + + Q G+
Sbjct: 647 EIEMLANREILANKPIKWEWMDRIEAERKFGFRLYQGGVPPGRKIRVVQVGDDVQACGGT 706
Query: 243 YRRDTGEV 266
+ R TGE+
Sbjct: 707 HCRSTGEI 714
>sp|P77206|YAFZ_ECOLI Hypothetical protein yafZ
Length = 273
Score = 28.5 bits (62), Expect = 5.3
Identities = 17/52 (32%), Positives = 26/52 (50%), Gaps = 4/52 (7%)
Frame = +3
Query: 141 GKKFPENAIMGGHDG----DKMLFIGRACVENQILVGSYRRDTGEVHIPFKG 284
GK+ PE ++ HDG + + RA +N ++ G GEV +P KG
Sbjct: 95 GKQVPEIILLNSHDGTSSYQMLPGLFRAVCQNGLVCG---ESFGEVRVPHKG 143
>sp|P41815|BAP3_YEAST Valine amino-acid permease (Branched-chain amino-acid permease 3)
Length = 604
Score = 28.1 bits (61), Expect = 7.0
Identities = 14/51 (27%), Positives = 25/51 (49%)
Frame = -2
Query: 362 DFMSYSM**LFINGAQNITAFHYVFFALEWYMHFTGISSVGSN*NLIFNTC 210
+ ++ SM + N N F +F+ ++HF G+ + G IFN+C
Sbjct: 187 ELITSSMTVKYWNDTINADVFIVIFYVFLLFIHFFGVKAYGET-EFIFNSC 236
>sp|P52132|YFJQ_ECOLI Hypothetical protein yfjQ
Length = 273
Score = 28.1 bits (61), Expect = 7.0
Identities = 17/52 (32%), Positives = 26/52 (50%), Gaps = 4/52 (7%)
Frame = +3
Query: 141 GKKFPENAIMGGHDG----DKMLFIGRACVENQILVGSYRRDTGEVHIPFKG 284
GK+ PE ++ HDG + + RA +N ++ G GEV +P KG
Sbjct: 95 GKQVPEIILLNSHDGTSSYQMLPGMFRAVCQNGLVCG---ESFGEVRVPHKG 143
>sp|P19828|ANGR_VIBAN Protein angR
Length = 1048
Score = 27.7 bits (60), Expect = 9.1
Identities = 20/79 (25%), Positives = 38/79 (48%), Gaps = 1/79 (1%)
Frame = +3
Query: 39 YCGYKGVAYSHYEYQIFVETEIKNNTKLRWKWTDGKKFPENAIMGGHDGDKML-FIGRAC 215
+ G G+A +++ ++ T+ ++ DG + MG + D L F+GR
Sbjct: 798 WIGGDGIALGYFDDEL--------KTQAQFLHIDGHAWYRTGDMGCYWPDGTLEFLGRR- 848
Query: 216 VENQILVGSYRRDTGEVHI 272
+ Q+ VG YR + GE+ +
Sbjct: 849 -DKQVKVGGYRIELGEIEV 866
>sp|Q46266|PFL_CLOPA Formate acetyltransferase (Pyruvate formate-lyase)
Length = 740
Score = 27.7 bits (60), Expect = 9.1
Identities = 10/32 (31%), Positives = 16/32 (50%)
Frame = +3
Query: 213 CVENQILVGSYRRDTGEVHIPFKGKEYIVKCC 308
C E IL S + + ++ P G +Y + CC
Sbjct: 375 CAEMSILTDSIQYENDDIMRPIYGDDYAIACC 406
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 51,719,322
Number of Sequences: 369166
Number of extensions: 1076820
Number of successful extensions: 2161
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 2143
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2161
length of database: 68,354,980
effective HSP length: 98
effective length of database: 50,250,950
effective search space used: 1809034200
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)