Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_008_K05
(613 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O19010|CRIS3_HORSE Cysteine-rich secretory protein 3 pre... 124 2e-28
sp|P35782|VA52_VESCR Venom allergen 5.02 (Antigen 5-2) (Ag5... 120 2e-27
sp|P81657|VA5_VESMA Venom allergen 5 (Antigen 5) (Ag5) (All... 120 2e-27
sp|P54108|CRIS3_HUMAN Cysteine-rich secretory protein 3 pre... 120 3e-27
sp|Q91055|HELO_HELHO Helothermine precursor (HLTx) 120 4e-27
sp|P35760|VA5_VESMC Venom allergen 5 (Antigen 5) (Ag5) (All... 119 6e-27
sp|P35783|VA5_VESFL Venom allergen 5 (Antigen 5) (Ag5) (All... 119 8e-27
sp|Q60477|CRIS2_CAVPO Cysteine-rich secretory protein 2 pre... 118 1e-26
sp|P35781|VA51_VESCR Venom allergen 5.01 (Antigen 5-1) (Ag5... 117 2e-26
sp|Q8AVA4|CRVP_PSEAU Pseudechetoxin precursor (PsTx) 117 2e-26
>sp|O19010|CRIS3_HORSE Cysteine-rich secretory protein 3 precursor (CRISP-3)
Length = 245
Score = 124 bits (311), Expect = 2e-28
Identities = 64/177 (36%), Positives = 102/177 (57%), Gaps = 4/177 (2%)
Frame = +2
Query: 50 QKQLIDAHNKFRRDVAQGKVSGQPNAANMLELKWNNEAAQRAQNYANRCVFGHDQSKDRK 229
QK++++ HN RR V+ P A+NML+++W+++ A AQN+AN+C+ H +++DR
Sbjct: 38 QKEIVNKHNDLRRTVS-------PLASNMLKMQWDSKTATNAQNWANKCLLQHSKAEDRA 90
Query: 230 FGSYDWVGQNIAYWS-SNDVTEGIQMWFDEVKGYTFN-NDRCTGFTCLHYTALVWAKTKE 403
G+ G+N+ S N ++ IQ W DEV + + + HYT +VW +
Sbjct: 91 VGTMK-CGENLFMSSIPNSWSDAIQNWHDEVHDFKYGVGPKTPNAVVGHYTQVVWYSSYR 149
Query: 404 FGCGIADCSRQNLGKIFLICNYGPGGNWNNE--KPYETGSACSKCPSGTRCNNSLCS 568
GCGIA C +Q K + +C Y P GN+ N+ PYE G+ C++CP C+N LC+
Sbjct: 150 VGCGIAYCPKQGTLKYYYVCQYCPAGNYVNKINTPYEQGTPCARCPG--NCDNGLCT 204
>sp|P35782|VA52_VESCR Venom allergen 5.02 (Antigen 5-2) (Ag5-2) (Allergen Vesp c 5.02)
(Vesp c V.02)
Length = 202
Score = 120 bits (302), Expect = 2e-27
Identities = 69/179 (38%), Positives = 99/179 (55%), Gaps = 10/179 (5%)
Frame = +2
Query: 2 NSARGSVCQCAVSPAQQKQLIDAHNKFRRDVAQG-----KVSGQPNAANMLELKWNNEAA 166
N + V ++ + +++ HN+FR+ VA+G QP A +M L WN+E A
Sbjct: 25 NCGKNVVKASGLTKQENLEILKQHNEFRQKVARGLETRGNPGPQPPAKSMNTLVWNDELA 84
Query: 167 QRAQNYANRCVFGHDQSKDRKFGSYDWVGQNIAYWSSN-----DVTEGIQMWFDEVKGYT 331
Q AQ +AN+C +GHD ++ S VGQNIA S++ +V+ ++MW DEVK Y
Sbjct: 85 QIAQVWANQCNYGHDNCRNSAKYS---VGQNIAEGSTSADNFVNVSNMVKMWEDEVKDYQ 141
Query: 332 FNNDRCTGFTCLHYTALVWAKTKEFGCGIADCSRQNLGKIFLICNYGPGGNWNNEKPYE 508
+ + + HYT +VWAKTKE GCG D + +L+CNYGP GN NE YE
Sbjct: 142 YGSPKNKLNKVGHYTQMVWAKTKEIGCGSEDYIEDGWHRHYLVCNYGPAGNVGNEPIYE 200
>sp|P81657|VA5_VESMA Venom allergen 5 (Antigen 5) (Ag5) (Allergen Vesp m 5)
Length = 202
Score = 120 bits (302), Expect = 2e-27
Identities = 70/179 (39%), Positives = 97/179 (54%), Gaps = 10/179 (5%)
Frame = +2
Query: 2 NSARGSVCQCAVSPAQQKQLIDAHNKFRRDVAQG-----KVSGQPNAANMLELKWNNEAA 166
N + V ++ A++ +++ HN+FR+ VA+G K QP A +M L WN+E A
Sbjct: 25 NCGKNVVKASGLTKAEKLEILKQHNEFRQKVARGLETRGKPGPQPPAKSMNTLVWNDELA 84
Query: 167 QRAQNYANRCVFGHDQSKDRKFGSYDWVGQNIAYWSSN-----DVTEGIQMWFDEVKGYT 331
Q AQ +A +C +GHD ++ S VGQNIA S V+ +QMW DEVK Y
Sbjct: 85 QIAQVWAGQCDYGHDVCRNTAKYS---VGQNIAENGSTAASFASVSNMVQMWADEVKNYQ 141
Query: 332 FNNDRCTGFTCLHYTALVWAKTKEFGCGIADCSRQNLGKIFLICNYGPGGNWNNEKPYE 508
+ + + HYT +VWAKTKE GCG + +L+CNYGP GN NE YE
Sbjct: 142 YGSTKNKLIEVGHYTQMVWAKTKEIGCGSIKYIENGWHRHYLVCNYGPAGNIGNEPIYE 200
>sp|P54108|CRIS3_HUMAN Cysteine-rich secretory protein 3 precursor (CRISP-3) (SGP28
protein)
Length = 245
Score = 120 bits (301), Expect = 3e-27
Identities = 65/180 (36%), Positives = 94/180 (52%), Gaps = 7/180 (3%)
Frame = +2
Query: 50 QKQLIDAHNKFRRDVAQGKVSGQPNAANMLELKWNNEAAQRAQNYANRCVFGHDQSKDR- 226
Q+++++ HN+ RR V+ P A NML+++WN EAA AQ +AN+C + H KDR
Sbjct: 39 QREIVNKHNELRRAVS-------PPARNMLKMEWNKEAAANAQKWANQCNYRHSNPKDRM 91
Query: 227 ---KFGSYDWVGQNIAYWSSNDVTEGIQMWFDEVKGYTFN-NDRCTGFTCLHYTALVWAK 394
K G ++ + WS + IQ WFDE + F + HYT +VW
Sbjct: 92 TSLKCGENLYMSSASSSWS-----QAIQSWFDEYNDFDFGVGPKTPNAVVGHYTQVVWYS 146
Query: 395 TKEFGCGIADCSRQNLGKIFLICNYGPGGNWNNE--KPYETGSACSKCPSGTRCNNSLCS 568
+ GCG A C Q + K + +C Y P GNW N PYE G+ C+ CP C++ LC+
Sbjct: 147 SYLVGCGNAYCPNQKVLKYYYVCQYCPAGNWANRLYVPYEQGAPCASCPD--NCDDGLCT 204
>sp|Q91055|HELO_HELHO Helothermine precursor (HLTx)
Length = 242
Score = 120 bits (300), Expect = 4e-27
Identities = 69/182 (37%), Positives = 96/182 (52%), Gaps = 5/182 (2%)
Frame = +2
Query: 38 SPAQQKQLIDAHNKFRRDVAQGKVSGQPNAANMLELKWNNEAAQRAQNYANRCVFGHDQS 217
+P QQ ++ D HN RR V +P A+NML++ W+N+ AQ AQ AN+C H
Sbjct: 32 NPDQQTEITDKHNNLRRIV-------EPTASNMLKMTWSNKIAQNAQRSANQCTLEHTSK 84
Query: 218 KDRKFGSYDWVGQNIAYWSSNDVT--EGIQMWFDEVKGYTFNNDRCTGFTCL-HYTALVW 388
++R + G+N+ ++SS T IQ WFDE K + FN + HYT +VW
Sbjct: 85 EERTIDGVE-CGENL-FFSSAPYTWSYAIQNWFDERKYFRFNYGPTAQNVMIGHYTQVVW 142
Query: 389 AKTKEFGCGIADCSRQNLGKIFLICNYGPGGNWNNEK--PYETGSACSKCPSGTRCNNSL 562
++ E GC IA C Q K + +C Y PGGN + K PY G C CP C+N L
Sbjct: 143 YRSYELGCAIAYCPDQPTYKYYQVCQYCPGGNIRSRKYTPYSIGPPCGDCPDA--CDNGL 200
Query: 563 CS 568
C+
Sbjct: 201 CT 202
>sp|P35760|VA5_VESMC Venom allergen 5 (Antigen 5) (Ag5) (Allergen Ves m 5) (Ves m V)
Length = 204
Score = 119 bits (298), Expect = 6e-27
Identities = 71/186 (38%), Positives = 100/186 (53%), Gaps = 16/186 (8%)
Frame = +2
Query: 2 NSARGSVCQCAVSPAQQKQLIDAHNKFRRDVAQG-----KVSGQPNAANMLELKWNNEAA 166
N V ++ +++ ++ HN FR+ +A+G QP A NM L W++E A
Sbjct: 25 NCGNKKVVSYGLTKQEKQDILKEHNDFRQKIARGLETRGNPGPQPPAKNMKNLVWSDELA 84
Query: 167 QRAQNYANRCVFGHDQSKDRKFGSYDWVGQNIAYWSS-----NDVTEGIQMWFDEVKGY- 328
AQ +AN+C +GHD +D Y VGQN+A S ND + ++MW DEVK Y
Sbjct: 85 YIAQVWANQCQYGHDTCRD--VAKYQ-VGQNVALTGSTAAVYNDPVKLVKMWEDEVKDYN 141
Query: 329 -----TFNNDRCTGFTCLHYTALVWAKTKEFGCGIADCSRQNLGKIFLICNYGPGGNWNN 493
+ NN G HYT +VWA TKE GCG ++N K +L+CNYGP GN+ N
Sbjct: 142 PKKKFSENNFLKIG----HYTQMVWANTKEVGCGSIKYIQENWHKHYLVCNYGPSGNFQN 197
Query: 494 EKPYET 511
E+ Y+T
Sbjct: 198 EELYQT 203
>sp|P35783|VA5_VESFL Venom allergen 5 (Antigen 5) (Ag5) (Allergen Ves f 5) (Ves f V)
Length = 204
Score = 119 bits (297), Expect = 8e-27
Identities = 71/186 (38%), Positives = 100/186 (53%), Gaps = 16/186 (8%)
Frame = +2
Query: 2 NSARGSVCQCAVSPAQQKQLIDAHNKFRRDVAQG-----KVSGQPNAANMLELKWNNEAA 166
N V ++ +++ ++ HN FR+ +A+G QP A NM L WN+E A
Sbjct: 25 NCGNKVVVSYGLTKQEKQDILKEHNDFRQKIARGLETRGNPGPQPPAKNMKNLVWNDELA 84
Query: 167 QRAQNYANRCVFGHDQSKDRKFGSYDWVGQNIAYWSS-----NDVTEGIQMWFDEVKGY- 328
AQ +AN+C +GHD +D Y VGQN+A S +D + ++MW DEVK Y
Sbjct: 85 YVAQVWANQCQYGHDTCRD--IAKYQ-VGQNVALTGSTAAKYDDPVKLVKMWEDEVKDYN 141
Query: 329 -----TFNNDRCTGFTCLHYTALVWAKTKEFGCGIADCSRQNLGKIFLICNYGPGGNWNN 493
+ NN TG HYT +VWA TKE GCG ++ K +L+CNYGP GN+ N
Sbjct: 142 PKKKFSGNNFLKTG----HYTQMVWANTKEVGCGSIKFIQEKWHKHYLVCNYGPSGNFQN 197
Query: 494 EKPYET 511
E+ Y+T
Sbjct: 198 EELYQT 203
>sp|Q60477|CRIS2_CAVPO Cysteine-rich secretory protein 2 precursor (CRISP-2)
(Testis-specific protein TPX-1) (Autoantigen 1) (25 kDa
acrosomal autoantigen) (AA1)
Length = 244
Score = 118 bits (296), Expect = 1e-26
Identities = 67/178 (37%), Positives = 95/178 (53%), Gaps = 5/178 (2%)
Frame = +2
Query: 50 QKQLIDAHNKFRRDVAQGKVSGQPNAANMLELKWNNEAAQRAQNYANRCVFGHDQSKDRK 229
Q ++I+ HN+ R+ V P A+NML+++W+ EAA AQ +ANRC H DRK
Sbjct: 38 QNEIINKHNQLRKSVT-------PPASNMLKMEWSREAAVNAQKWANRCTLVHSNPDDRK 90
Query: 230 FGSYDWVGQNIAYWSS--NDVTEGIQMWFDEVKGYTFN-NDRCTGFTCLHYTALVWAKTK 400
+ G+N+ Y SS + ++ IQ WFDE + +TF + HYT LVW +
Sbjct: 91 TSTK--CGENL-YMSSDPSSWSDAIQSWFDESQDFTFGVGPKSHNAVVGHYTQLVWYSSY 147
Query: 401 EFGCGIADCSRQNLGKIFLICNYGPGGN--WNNEKPYETGSACSKCPSGTRCNNSLCS 568
GCGIA C Q+ K + +C Y P GN + PY+ G C+ CP C N LC+
Sbjct: 148 LVGCGIAYCPNQDSLKYYYVCQYCPAGNNVYTKNTPYKQGIPCASCPG--HCENGLCT 203
>sp|P35781|VA51_VESCR Venom allergen 5.01 (Antigen 5-1) (Ag5-1) (Allergen Vesp c 5.01)
(Vesp c V.01)
Length = 202
Score = 117 bits (293), Expect = 2e-26
Identities = 68/179 (37%), Positives = 96/179 (53%), Gaps = 10/179 (5%)
Frame = +2
Query: 2 NSARGSVCQCAVSPAQQKQLIDAHNKFRRDVAQG-----KVSGQPNAANMLELKWNNEAA 166
N + V ++ + +++ HN+FR+ VA+G QP A +M L WN+E A
Sbjct: 25 NCGKNVVKASGLTKQENLEILKQHNEFRQKVARGLETRGNPGPQPPAKSMNTLVWNDELA 84
Query: 167 QRAQNYANRCVFGHDQSKDRKFGSYDWVGQNIAYWSSN-----DVTEGIQMWFDEVKGYT 331
Q AQ +AN+C +GHD ++ S VGQNIA S+ V+ ++MW DEVK Y
Sbjct: 85 QIAQVWANQCNYGHDNCRNSAKYS---VGQNIAEGSTTADNFGSVSNMVKMWEDEVKDYQ 141
Query: 332 FNNDRCTGFTCLHYTALVWAKTKEFGCGIADCSRQNLGKIFLICNYGPGGNWNNEKPYE 508
+ + + HYT +VWAKTKE GCG + +L+CNYGP GN NE YE
Sbjct: 142 YGSPKNKLNKVGHYTQMVWAKTKEIGCGSIKYIENGWHRHYLVCNYGPAGNVGNEPIYE 200
>sp|Q8AVA4|CRVP_PSEAU Pseudechetoxin precursor (PsTx)
Length = 238
Score = 117 bits (293), Expect = 2e-26
Identities = 67/177 (37%), Positives = 90/177 (50%), Gaps = 4/177 (2%)
Frame = +2
Query: 50 QKQLIDAHNKFRRDVAQGKVSGQPNAANMLELKWNNEAAQRAQNYANRCVFGHDQSKDRK 229
QK+++D HN RR V +P A NML++KWN+ AAQ A+ +ANRC F H R
Sbjct: 34 QKEIVDKHNALRRSV-------KPTARNMLQMKWNSRAAQNAKRWANRCTFAHSPPNKRT 86
Query: 230 FGSYDWVGQNIAYWSSNDVTEG-IQMWFDEVKGYTFN-NDRCTGFTCLHYTALVWAKTKE 403
G G+NI S G +Q W+DE+K + + + G HYT +VW K+
Sbjct: 87 VGKLR-CGENIFMSSQPFPWSGVVQAWYDEIKNFVYGIGAKPPGSVIGHYTQVVWYKSYL 145
Query: 404 FGCGIADCSRQNLGKIFLICNYGPGGNWNNE--KPYETGSACSKCPSGTRCNNSLCS 568
GC A CS K +C Y P GN PY++G C+ CPS C N LC+
Sbjct: 146 IGCASAKCSS---SKYLYVCQYCPAGNIRGSIATPYKSGPPCADCPSA--CVNKLCT 197
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 72,271,137
Number of Sequences: 369166
Number of extensions: 1511567
Number of successful extensions: 3861
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3554
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3671
length of database: 68,354,980
effective HSP length: 105
effective length of database: 48,957,805
effective search space used: 4797864890
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)