Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_008_K04
(284 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q8HEC1|NU2M_CAEBR NADH-ubiquinone oxidoreductase chain 2... 31 0.77
sp|Q8D2Q5|DNAK_WIGBR Chaperone protein dnaK (Heat shock pro... 30 2.2
sp|P47685|Y447_MYCGE Hypothetical protein MG447 29 2.9
sp|P24889|NU2M_CAEEL NADH-ubiquinone oxidoreductase chain 2... 29 3.8
sp|P53220|YG1M_YEAST Hypothetical 27.2 kDa protein in GLS2-... 28 5.0
sp|P34428|YL37_CAEEL Hypothetical protein F44B9.7 in chromo... 28 6.5
sp|P29817|VG1L_AMEPV G1L protein 28 8.5
sp|P16237|HMDH_SCHMA 3-hydroxy-3-methylglutaryl-coenzyme A ... 28 8.5
sp|P0C0D1|RECF_STRP1 DNA replication and repair protein recF 28 8.5
>sp|Q8HEC1|NU2M_CAEBR NADH-ubiquinone oxidoreductase chain 2 (NADH dehydrogenase subunit
2)
Length = 282
Score = 31.2 bits (69), Expect = 0.77
Identities = 20/71 (28%), Positives = 34/71 (47%)
Frame = -1
Query: 275 IYF*KLFFLIIEQFSVSRFYFSLFHPSNSFIISLCFSGDNFSKVFIKSSA*SLSDLFKNK 96
+Y+ L L+I +FS S Y + + +++ FS F K+F S F
Sbjct: 176 VYYFLLMILLISKFSKSSSYNFVNWETALVFLNIPFSVSFFVKIFSLSEIFKFDSFFTLL 235
Query: 95 CIRSIFLSIIA 63
+ S+FLS++A
Sbjct: 236 LLFSMFLSVLA 246
>sp|Q8D2Q5|DNAK_WIGBR Chaperone protein dnaK (Heat shock protein 70) (Heat shock 70 kDa
protein) (HSP70)
Length = 645
Score = 29.6 bits (65), Expect = 2.2
Identities = 15/31 (48%), Positives = 18/31 (58%)
Frame = -1
Query: 98 KCIRSIFLSIIAETLNEYEINTASKIQIASC 6
KCI + LSI E + EI S IQI+SC
Sbjct: 570 KCINELELSIKGEDKKDIEIKIQSLIQISSC 600
>sp|P47685|Y447_MYCGE Hypothetical protein MG447
Length = 547
Score = 29.3 bits (64), Expect = 2.9
Identities = 14/33 (42%), Positives = 21/33 (63%)
Frame = -1
Query: 281 KNIYF*KLFFLIIEQFSVSRFYFSLFHPSNSFI 183
KN++ K FL I +F+V F+F+LF + FI
Sbjct: 15 KNVFESKQLFLTILRFTVPTFFFALFSAAYVFI 47
>sp|P24889|NU2M_CAEEL NADH-ubiquinone oxidoreductase chain 2 (NADH dehydrogenase subunit
2)
Length = 282
Score = 28.9 bits (63), Expect = 3.8
Identities = 19/71 (26%), Positives = 34/71 (47%)
Frame = -1
Query: 275 IYF*KLFFLIIEQFSVSRFYFSLFHPSNSFIISLCFSGDNFSKVFIKSSA*SLSDLFKNK 96
IY+ L L+I +FS + Y + + +++ FS F K+F S F
Sbjct: 176 IYYFVLMVLLISKFSKTSGYNFINWETTLVFLNIPFSVSFFVKIFSLSEIFKYDSFFTLF 235
Query: 95 CIRSIFLSIIA 63
+ ++FLS++A
Sbjct: 236 LLFTMFLSVLA 246
>sp|P53220|YG1M_YEAST Hypothetical 27.2 kDa protein in GLS2-RPL26B intergenic region
Length = 239
Score = 28.5 bits (62), Expect = 5.0
Identities = 13/37 (35%), Positives = 22/37 (59%)
Frame = +1
Query: 76 KKMERMHLFLKRSDRDYADDFMNTFEKLSPEKQRLII 186
KK+ +HL K S ++Y DF+N + + EK+ +I
Sbjct: 180 KKIALVHLEAKESKQNYQPDFINMYVDVPGEKRYYLI 216
>sp|P34428|YL37_CAEEL Hypothetical protein F44B9.7 in chromosome III
Length = 466
Score = 28.1 bits (61), Expect = 6.5
Identities = 16/51 (31%), Positives = 24/51 (47%)
Frame = +1
Query: 58 VSAIMDKKMERMHLFLKRSDRDYADDFMNTFEKLSPEKQRLIINELDGWKR 210
+S I+ K MER L + D AD N +++S +Q + WKR
Sbjct: 303 LSTILKKVMERKPLNQGENPNDLADQCKNALQRMSDIRQIIEKRREPTWKR 353
>sp|P29817|VG1L_AMEPV G1L protein
Length = 464
Score = 27.7 bits (60), Expect = 8.5
Identities = 21/71 (29%), Positives = 33/71 (46%)
Frame = +1
Query: 46 YSFSVSAIMDKKMERMHLFLKRSDRDYADDFMNTFEKLSPEKQRLIINELDGWKREK*NR 225
YS S + R LF+ R D + D+F NT E + + ++ + EL ++ NR
Sbjct: 380 YSMSFIGDLLASSYRGALFISRYDINSIDEFKNTLEIFNIKNKKFM--ELIDMYKKNSNR 437
Query: 226 ETENCSIIKKN 258
CS IK +
Sbjct: 438 IMNVCSKIKND 448
>sp|P16237|HMDH_SCHMA 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMG-CoA reductase)
Length = 948
Score = 27.7 bits (60), Expect = 8.5
Identities = 18/73 (24%), Positives = 35/73 (47%), Gaps = 4/73 (5%)
Frame = -1
Query: 260 LFFLIIEQFSVSRFYFSLFHPSNSFIISLCFSG----DNFSKVFIKSSA*SLSDLFKNKC 93
+FF+++E + + + H ++S FS D+ +F+K+S S+S K
Sbjct: 135 VFFIVLEYYLLEIELVNYEHAKRHCLLSHLFSNQLFVDHMLGMFLKTSLFSISTTSKYAY 194
Query: 92 IRSIFLSIIAETL 54
+ SIF + E +
Sbjct: 195 LESIFKCTLMEQI 207
>sp|P0C0D1|RECF_STRP1 DNA replication and repair protein recF
Length = 368
Score = 27.7 bits (60), Expect = 8.5
Identities = 12/47 (25%), Positives = 22/47 (46%)
Frame = +1
Query: 40 ISYSFSVSAIMDKKMERMHLFLKRSDRDYADDFMNTFEKLSPEKQRL 180
+S S+ S + DKK FL + ++++ DF + P + L
Sbjct: 218 LSLSYQSSVVFDKKTNIYQQFLHQLEKNHQKDFFRKNTSVGPHRDEL 264
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 25,800,380
Number of Sequences: 369166
Number of extensions: 414532
Number of successful extensions: 1144
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 1131
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1144
length of database: 68,354,980
effective HSP length: 64
effective length of database: 56,531,940
effective search space used: 1695958200
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)