Planarian EST Database


Dr_sW_008_G04

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_008_G04
         (855 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|O18767|MMP20_BOVIN  Matrix metalloproteinase-20 precursor...   133   5e-31
sp|O60882|MMP20_HUMAN  Matrix metalloproteinase-20 precursor...   132   1e-30
sp|P79287|MMP20_PIG  Matrix metalloproteinase-20 precursor (...   131   3e-30
sp|P57748|MMP20_MOUSE  Matrix metalloproteinase-20 precursor...   129   8e-30
sp|Q9JHI0|MMP19_MOUSE  Matrix metalloproteinase-19 precursor...   127   5e-29
sp|Q9R0S2|MMP24_MOUSE  Matrix metalloproteinase-24 precursor...   126   7e-29
sp|Q99PW6|MMP24_RAT  Matrix metalloproteinase-24 precursor (...   126   7e-29
sp|P09237|MMP7_HUMAN  Matrilysin precursor (Pump-1 protease)...   126   9e-29
sp|Q9Y5R2|MMP24_HUMAN  Matrix metalloproteinase-24 precursor...   124   3e-28
sp|Q9NPA2|MMP25_HUMAN  Matrix metalloproteinase-25 precursor...   124   3e-28
>sp|O18767|MMP20_BOVIN Matrix metalloproteinase-20 precursor (MMP-20) (Enamel
           metalloproteinase) (Enamelysin)
          Length = 481

 Score =  133 bits (335), Expect = 5e-31
 Identities = 85/224 (37%), Positives = 120/224 (53%), Gaps = 4/224 (1%)
 Frame = +2

Query: 125 VKNIQKMAGLPTDGKINKELLAHVGKSRCGGKDVKPKNKRYNAANSKWDKGT-TYRFYIH 301
           +K +Q+  GL   GK+++  +  + + RCG  DV   N R      KW K T TYR    
Sbjct: 70  IKELQEFFGLRVTGKLDRATMDVIKRPRCGVPDVA--NYRLFPGEPKWKKNTLTYRI--- 124

Query: 302 DERHPTTMTLGEIQRALMIGLSYWSTAGDIYFAKVSNKQGSMFSLSFADAEHVQYDVHNN 481
             ++  +MT  E+ RA+ + L  WS+A  + F +++  +  +  +SF   +H        
Sbjct: 125 -SKYTPSMTPAEVDRAMEMALRAWSSAVPLNFVRINAGEADIM-ISFETGDH-------- 174

Query: 482 FVESRDPYPLDNVPGGVLAHAFFPRNG---NLHFDSAEEWTEGKEDGINLRLVAAHEMGH 652
                D YP D  P G LAHAF P  G   + HFD+AE+WT G  +G NL  VAAHE GH
Sbjct: 175 ----GDSYPFDG-PRGTLAHAFAPGEGLGGDTHFDNAEKWTMGT-NGFNLFTVAAHEFGH 228

Query: 653 AIGLFHSNDPNALMAPWYGGYISENDFKLPDDDSQGAAFIYGTK 784
           A+GL HS DP+ALM P Y  Y +   F+LP DD +G   +YG +
Sbjct: 229 ALGLAHSTDPSALMFPTY-KYQNPYGFRLPKDDVKGIQALYGPR 271
>sp|O60882|MMP20_HUMAN Matrix metalloproteinase-20 precursor (MMP-20) (Enamel
           metalloproteinase) (Enamelysin)
          Length = 483

 Score =  132 bits (332), Expect = 1e-30
 Identities = 87/230 (37%), Positives = 124/230 (53%), Gaps = 4/230 (1%)
 Frame = +2

Query: 125 VKNIQKMAGLPTDGKINKELLAHVGKSRCGGKDVKPKNKRYNAANSKWDKGT-TYRFYIH 301
           +K +Q   GL   GK+++  +  + K RCG  DV   N R      KW K T TYR    
Sbjct: 72  IKELQAFFGLQVTGKLDQTTMNVIKKPRCGVPDVA--NYRLFPGEPKWKKNTLTYRI--- 126

Query: 302 DERHPTTMTLGEIQRALMIGLSYWSTAGDIYFAKVSNKQGSMFSLSFADAEHVQYDVHNN 481
             ++  +M+  E+ +A+ + L  WS+A  + F ++++ +  +  +SF + +H        
Sbjct: 127 -SKYTPSMSSVEVDKAVEMALQAWSSAVPLSFVRINSGEADIM-ISFENGDH-------- 176

Query: 482 FVESRDPYPLDNVPGGVLAHAFFPRNG---NLHFDSAEEWTEGKEDGINLRLVAAHEMGH 652
                D YP D  P G LAHAF P  G   + HFD+AE+WT G  +G NL  VAAHE GH
Sbjct: 177 ----GDSYPFDG-PRGTLAHAFAPGEGLGGDTHFDNAEKWTMGT-NGFNLFTVAAHEFGH 230

Query: 653 AIGLFHSNDPNALMAPWYGGYISENDFKLPDDDSQGAAFIYGTKNGKVFV 802
           A+GL HS DP+ALM P Y  Y +   F LP DD +G   +YG +  KVF+
Sbjct: 231 ALGLAHSTDPSALMYPTY-KYKNPYGFHLPKDDVKGIQALYGPR--KVFL 277
>sp|P79287|MMP20_PIG Matrix metalloproteinase-20 precursor (MMP-20) (Enamel
           metalloproteinase) (Enamelysin)
          Length = 483

 Score =  131 bits (329), Expect = 3e-30
 Identities = 85/224 (37%), Positives = 118/224 (52%), Gaps = 4/224 (1%)
 Frame = +2

Query: 125 VKNIQKMAGLPTDGKINKELLAHVGKSRCGGKDVKPKNKRYNAANSKWDKGT-TYRFYIH 301
           +K +Q   GL   GK+++  +  + + RCG  DV   N R      KW K T TYR    
Sbjct: 72  IKELQAFFGLRVTGKLDRTTMDVIKRPRCGVPDVA--NYRLFPGEPKWKKNTLTYRI--- 126

Query: 302 DERHPTTMTLGEIQRALMIGLSYWSTAGDIYFAKVSNKQGSMFSLSFADAEHVQYDVHNN 481
             ++  +MT  E+ +A+ + L  WS+A  + F +V+  +  +  +SF   +H        
Sbjct: 127 -SKYTPSMTPAEVDKAMEMALQAWSSAVPLSFVRVNAGEADIM-ISFETGDH-------- 176

Query: 482 FVESRDPYPLDNVPGGVLAHAFFPRNG---NLHFDSAEEWTEGKEDGINLRLVAAHEMGH 652
                D YP D  P G LAHAF P  G   + HFD+AE+WT G  +G NL  VAAHE GH
Sbjct: 177 ----GDSYPFDG-PRGTLAHAFAPGEGLGGDTHFDNAEKWTMGM-NGFNLFTVAAHEFGH 230

Query: 653 AIGLFHSNDPNALMAPWYGGYISENDFKLPDDDSQGAAFIYGTK 784
           A+GL HS DP+ALM P Y  Y +   F LP DD +G   +YG +
Sbjct: 231 ALGLAHSTDPSALMYPTY-KYQNPYGFHLPKDDVKGIQALYGPR 273
>sp|P57748|MMP20_MOUSE Matrix metalloproteinase-20 precursor (MMP-20) (Enamel
           metalloproteinase) (Enamelysin)
          Length = 482

 Score =  129 bits (325), Expect = 8e-30
 Identities = 86/229 (37%), Positives = 121/229 (52%), Gaps = 4/229 (1%)
 Frame = +2

Query: 125 VKNIQKMAGLPTDGKINKELLAHVGKSRCGGKDVKPKNKRYNAANSKWDKGT-TYRFYIH 301
           +K +Q   GL   GK+++  +  + K RCG  DV   N R      KW K   TYR    
Sbjct: 71  IKELQIFFGLKVTGKLDQNTMNVIKKPRCGVPDVA--NYRLFPGEPKWKKNILTYRI--- 125

Query: 302 DERHPTTMTLGEIQRALMIGLSYWSTAGDIYFAKVSNKQGSMFSLSFADAEHVQYDVHNN 481
             ++  +M+  E+ +A+ + L  WSTA  + F ++++ +  +  +SF   +H        
Sbjct: 126 -SKYTPSMSPTEVDKAIQMALHAWSTAVPLNFVRINSGEADIM-ISFETGDH-------- 175

Query: 482 FVESRDPYPLDNVPGGVLAHAFFPRNG---NLHFDSAEEWTEGKEDGINLRLVAAHEMGH 652
                D YP D  P G LAHAF P  G   + HFD+AE+WT G  +G NL  VAAHE GH
Sbjct: 176 ----GDSYPFDG-PRGTLAHAFAPGEGLGGDTHFDNAEKWTMGT-NGFNLFTVAAHEFGH 229

Query: 653 AIGLFHSNDPNALMAPWYGGYISENDFKLPDDDSQGAAFIYGTKNGKVF 799
           A+GL HS DP+ALM P Y  Y +   F LP DD +G   +YG +  K+F
Sbjct: 230 ALGLGHSTDPSALMYPTY-KYQNPYRFHLPKDDVKGIQALYGPR--KIF 275
>sp|Q9JHI0|MMP19_MOUSE Matrix metalloproteinase-19 precursor (MMP-19) (Matrix
           metalloproteinase RASI)
          Length = 527

 Score =  127 bits (318), Expect = 5e-29
 Identities = 83/244 (34%), Positives = 126/244 (51%), Gaps = 1/244 (0%)
 Frame = +2

Query: 59  YVEKFLSDFYDYNENTDRLNDAVKNIQKMAGLPTDGKINKELLAHVGKSRCGGKD-VKPK 235
           Y++K L    D+    + + +A++  Q+ +GLP  G+++    A + + RCG +D    K
Sbjct: 38  YLQKPLEGADDFR--LEDITEALRTFQEASGLPISGQMDDATRARMKQPRCGLEDPFNQK 95

Query: 236 NKRYNAANSKWDKGTTYRFYIHDERHPTTMTLGEIQRALMIGLSYWSTAGDIYFAKVSNK 415
           + +Y        K  T+R +      P+T++L  ++ AL     YWS+   + F +V   
Sbjct: 96  SLKYLLLGHWRKKNLTFRIF----NVPSTLSLPRVRAALHQAFKYWSSVAPLTFREVKAG 151

Query: 416 QGSMFSLSFADAEHVQYDVHNNFVESRDPYPLDNVPGGVLAHAFFPRNGNLHFDSAEEWT 595
              +  LSF   + +     N F          + PG VLAHA  P  G++HFD  E WT
Sbjct: 152 WADI-RLSFHGRQSLYCS--NTF----------DGPGKVLAHADIPELGSIHFDKDELWT 198

Query: 596 EGKEDGINLRLVAAHEMGHAIGLFHSNDPNALMAPWYGGYISENDFKLPDDDSQGAAFIY 775
           EG   G+NLR++AAHE+GHA+GL HS    ALMAP Y GY  +  FKL  DD  G   +Y
Sbjct: 199 EGTYQGVNLRIIAAHEVGHALGLGHSRYTQALMAPVYAGY--QPFFKLHPDDVAGIQALY 256

Query: 776 GTKN 787
           G ++
Sbjct: 257 GKRS 260
>sp|Q9R0S2|MMP24_MOUSE Matrix metalloproteinase-24 precursor (MMP-24) (Membrane-type
           matrix metalloproteinase 5) (MT-MMP 5) (Membrane-type-5
           matrix metalloproteinase) (MT5-MMP) (MMP-21)
          Length = 618

 Score =  126 bits (317), Expect = 7e-29
 Identities = 88/241 (36%), Positives = 121/241 (50%), Gaps = 19/241 (7%)
 Frame = +2

Query: 113 LNDAVKNIQKMAGLPTDGKINKELLAHVGKSRCGGKDV-----KPKNKRYNAANSKW-DK 274
           L  AV  +Q+  G+P  G +++  +  + K RCG  D      + +NKRY     KW  K
Sbjct: 80  LQSAVSTMQQFYGIPVTGVLDQTTIEWMKKPRCGVPDHPHLSRRRRNKRYALTGQKWRQK 139

Query: 275 GTTYRFYIHDERHPTTMTLGEI--QRALMIGLSYWSTAGDIYFAKV------SNKQGSMF 430
             TY        H  T  +GE+  ++A+      W     + F +V      S+++ +  
Sbjct: 140 HITYSI------HNYTPKVGELDTRKAIRQAFDVWQKVTPLTFEEVPYHEIKSDRKEADI 193

Query: 431 SLSFADAEHVQYDVHNNFVESRDPYPLDNVPGGVLAHAFFPR---NGNLHFDSAEEWTEG 601
            + FA   H             D  P D   GG LAHA+FP     G+ HFDS E WT G
Sbjct: 194 MIFFASGFH------------GDSSPFDG-EGGFLAHAYFPGPGIGGDTHFDSDEPWTLG 240

Query: 602 K--EDGINLRLVAAHEMGHAIGLFHSNDPNALMAPWYGGYISENDFKLPDDDSQGAAFIY 775
               DG +L LVA HE+GHA+GL HSNDP+A+MAP+Y  Y+  ++FKLP DD QG   IY
Sbjct: 241 NANHDGNDLFLVAVHELGHALGLEHSNDPSAIMAPFY-QYMETHNFKLPQDDLQGIQKIY 299

Query: 776 G 778
           G
Sbjct: 300 G 300
>sp|Q99PW6|MMP24_RAT Matrix metalloproteinase-24 precursor (MMP-24) (Membrane-type
           matrix metalloproteinase 5) (MT-MMP 5) (Membrane-type-5
           matrix metalloproteinase) (MT5-MMP)
          Length = 618

 Score =  126 bits (317), Expect = 7e-29
 Identities = 88/241 (36%), Positives = 121/241 (50%), Gaps = 19/241 (7%)
 Frame = +2

Query: 113 LNDAVKNIQKMAGLPTDGKINKELLAHVGKSRCGGKDV-----KPKNKRYNAANSKW-DK 274
           L  AV  +Q+  G+P  G +++  +  + K RCG  D      + +NKRY     KW  K
Sbjct: 80  LQSAVSTMQQFYGIPVTGVLDQTTIEWMKKPRCGVPDHPHLSRRRRNKRYALTGQKWRQK 139

Query: 275 GTTYRFYIHDERHPTTMTLGEI--QRALMIGLSYWSTAGDIYFAKV------SNKQGSMF 430
             TY        H  T  +GE+  ++A+      W     + F +V      S+++ +  
Sbjct: 140 HITYSI------HNYTPKVGELDTRKAIRQAFDVWQKVTPLTFEEVPYHEIKSDRKEADI 193

Query: 431 SLSFADAEHVQYDVHNNFVESRDPYPLDNVPGGVLAHAFFPR---NGNLHFDSAEEWTEG 601
            + FA   H             D  P D   GG LAHA+FP     G+ HFDS E WT G
Sbjct: 194 MIFFASGFH------------GDSSPFDG-EGGFLAHAYFPGPGIGGDTHFDSDEPWTLG 240

Query: 602 K--EDGINLRLVAAHEMGHAIGLFHSNDPNALMAPWYGGYISENDFKLPDDDSQGAAFIY 775
               DG +L LVA HE+GHA+GL HSNDP+A+MAP+Y  Y+  ++FKLP DD QG   IY
Sbjct: 241 NANHDGNDLFLVAVHELGHALGLEHSNDPSAIMAPFY-QYMETHNFKLPQDDLQGIQKIY 299

Query: 776 G 778
           G
Sbjct: 300 G 300
>sp|P09237|MMP7_HUMAN Matrilysin precursor (Pump-1 protease) (Uterine metalloproteinase)
           (Matrix metalloproteinase-7) (MMP-7) (Matrin)
          Length = 267

 Score =  126 bits (316), Expect = 9e-29
 Identities = 88/250 (35%), Positives = 119/250 (47%), Gaps = 9/250 (3%)
 Frame = +2

Query: 65  EKFLSDFYDYNENTDRLNDA---VKNIQKMAGLPTDGKINKELLAHVGKSRCGGKDVKPK 235
           + +L  FY Y+  T   N     +K +QK  GLP  G +N  ++  + K RCG  DV   
Sbjct: 36  QDYLKRFYLYDSETKNANSLEAKLKEMQKFFGLPITGMLNSRVIEIMQKPRCGVPDVAEY 95

Query: 236 NKRYNAANSKW-DKGTTYRF--YIHDERHPTTMTLGEIQRALMIGLSYWSTAGDIYFAKV 406
           +   N+   KW  K  TYR   Y  D  H T      + R +   L+ W     ++F KV
Sbjct: 96  SLFPNSP--KWTSKVVTYRIVSYTRDLPHIT------VDRLVSKALNMWGKEIPLHFRKV 147

Query: 407 SNKQGSMFSLSFADAEHVQYDVHNNFVESRDPYPLDNVPGGVLAHAFFPRNG---NLHFD 577
                 +  + FA   H             D YP D  PG  LAHAF P  G   + HFD
Sbjct: 148 VWGTADIM-IGFARGAH------------GDSYPFDG-PGNTLAHAFAPGTGLGGDAHFD 193

Query: 578 SAEEWTEGKEDGINLRLVAAHEMGHAIGLFHSNDPNALMAPWYGGYISENDFKLPDDDSQ 757
             E WT+G   GIN    A HE+GH++G+ HS+DPNA+M P YG    +N FKL  DD +
Sbjct: 194 EDERWTDGSSLGINFLYAATHELGHSLGMGHSSDPNAVMYPTYGNGDPQN-FKLSQDDIK 252

Query: 758 GAAFIYGTKN 787
           G   +YG ++
Sbjct: 253 GIQKLYGKRS 262
>sp|Q9Y5R2|MMP24_HUMAN Matrix metalloproteinase-24 precursor (MMP-24) (Membrane-type
           matrix metalloproteinase 5) (MT-MMP 5) (Membrane-type-5
           matrix metalloproteinase) (MT5-MMP)
          Length = 645

 Score =  124 bits (312), Expect = 3e-28
 Identities = 87/241 (36%), Positives = 121/241 (50%), Gaps = 19/241 (7%)
 Frame = +2

Query: 113 LNDAVKNIQKMAGLPTDGKINKELLAHVGKSRCGGKDV-----KPKNKRYNAANSKW-DK 274
           L  AV  +Q+  G+P  G +++  +  + K RCG  D      + +NKRY     KW  K
Sbjct: 107 LQSAVSTMQQFYGIPVTGVLDQTTIEWMKKPRCGVPDHPHLSRRRRNKRYALTGQKWRQK 166

Query: 275 GTTYRFYIHDERHPTTMTLGEI--QRALMIGLSYWSTAGDIYFAKV------SNKQGSMF 430
             TY        H  T  +GE+  ++A+      W     + F +V      S+++ +  
Sbjct: 167 HITYSI------HNYTPKVGELDTRKAIRQAFDVWQKVTPLTFEEVPYHEIKSDRKEADI 220

Query: 431 SLSFADAEHVQYDVHNNFVESRDPYPLDNVPGGVLAHAFFPR---NGNLHFDSAEEWTEG 601
            + FA   H             D  P D   GG LAHA+FP     G+ HFDS E WT G
Sbjct: 221 MIFFASGFH------------GDSSPFDG-EGGFLAHAYFPGPGIGGDTHFDSDEPWTLG 267

Query: 602 K--EDGINLRLVAAHEMGHAIGLFHSNDPNALMAPWYGGYISENDFKLPDDDSQGAAFIY 775
               DG +L LVA HE+GHA+GL HS+DP+A+MAP+Y  Y+  ++FKLP DD QG   IY
Sbjct: 268 NANHDGNDLFLVAVHELGHALGLEHSSDPSAIMAPFY-QYMETHNFKLPQDDLQGIQKIY 326

Query: 776 G 778
           G
Sbjct: 327 G 327
>sp|Q9NPA2|MMP25_HUMAN Matrix metalloproteinase-25 precursor (MMP-25) (Membrane-type
           matrix metalloproteinase 6) (MT-MMP 6) (Membrane-type-6
           matrix metalloproteinase) (MT6-MMP) (Leukolysin)
          Length = 562

 Score =  124 bits (311), Expect = 3e-28
 Identities = 88/245 (35%), Positives = 125/245 (51%), Gaps = 18/245 (7%)
 Frame = +2

Query: 98  ENTDRLNDAVKNIQKMAGLPTDGKINKELLAHVGKSRCGGKDVKP------KNKRYNAAN 259
           ++ ++L DA+K +Q+ AGLP  G+++   +A + K RC   DV        + +RY  + 
Sbjct: 53  QSPEKLRDAIKVMQRFAGLPETGRMDPGTVATMRKPRCSLPDVLGVAGLVRRRRRYALSG 112

Query: 260 SKWDKGT-TYRFYIHDERHPTTMTLG-EIQRALM-IGLSYWSTAGDIYFAKVSNKQGSM- 427
           S W K T T+R        P +  L  E  R LM   L  W     + F +V + QG   
Sbjct: 113 SVWKKRTLTWRV----RSFPQSSQLSQETVRVLMSYALMAWGMESGLTFHEVDSPQGQEP 168

Query: 428 -FSLSFADAEHVQYDVHNNFVESRDPYPLDNVPGGVLAHAFFPR----NGNLHFDSAEEW 592
              + FA A H            +D YP D + GG LAHAFFP     +G+ HFD  E W
Sbjct: 169 DILIDFARAFH------------QDSYPFDGL-GGTLAHAFFPGEHPISGDTHFDDEETW 215

Query: 593 TEGKEDG--INLRLVAAHEMGHAIGLFHSNDPNALMAPWYGGYISEND-FKLPDDDSQGA 763
           T G +DG   +L  VA HE GHA+GL HS+ PN++M P+Y G + + D ++L  DD  G 
Sbjct: 216 TFGSKDGEGTDLFAVAVHEFGHALGLGHSSAPNSIMRPFYQGPVGDPDKYRLSQDDRDGL 275

Query: 764 AFIYG 778
             +YG
Sbjct: 276 QQLYG 280
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 100,267,793
Number of Sequences: 369166
Number of extensions: 2183397
Number of successful extensions: 6794
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 6262
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6583
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8438301375
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)