Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_008_E19
(824 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9JM53|PDCD8_RAT Programmed cell death protein 8, mitoch... 87 7e-17
sp|Q9Z0X1|PDCD8_MOUSE Programmed cell death protein 8, mito... 87 7e-17
sp|O95831|PDCD8_HUMAN Programmed cell death protein 8, mito... 86 9e-17
sp|Q9VQ79|PDCD8_DROME Putative oxidoreductase CG7263, mitoc... 86 9e-17
sp|O51597|SECF_BORBU Protein-export membrane protein secF 33 0.95
sp|Q9WTV1|CH3L1_RAT Chitinase-3-like protein 1 precursor (C... 32 2.8
sp|P58145|ROAA_ASTLO Ribosomal operon-associated A protein ... 30 6.2
sp|Q02137|ILVB_LACLA Acetolactate synthase large subunit (A... 30 6.2
sp|Q9WZH8|SYC_THEMA Cysteinyl-tRNA synthetase (Cysteine--tR... 30 8.1
sp|Q7VD64|TAL_PROMA Transaldolase 30 8.1
>sp|Q9JM53|PDCD8_RAT Programmed cell death protein 8, mitochondrial precursor
(Apoptosis-inducing factor)
Length = 612
Score = 86.7 bits (213), Expect = 7e-17
Identities = 69/228 (30%), Positives = 98/228 (42%), Gaps = 19/228 (8%)
Frame = +2
Query: 68 FLVNAELLAREGIYVAGDSCSFFDVNLGRRRVEHWNHSEESGSIAGHNMVAYVLKSVDAS 247
F VNAEL AR I+VAGD+ F+D+ LGRRRVEH +H+ SG +AG NM
Sbjct: 420 FRVNAELQARSNIWVAGDAACFYDIKLGRRRVEHHDHAVVSGRLAGENMTGAA------- 472
Query: 248 YFDSNSPKFVDNSNLIYDIFKPRIYNFQSSFAFKFNIYNSLEAIGLTDSKLET-KTFVAT 424
KP Y QS F EAIGL DS L T F
Sbjct: 473 --------------------KP--YWHQSMFWSDLGPDVGYEAIGLVDSSLPTVGVFAKA 510
Query: 425 DFYDDDFNIVESDEKVVEKALKPKTEHDPLDMP------------------GVVFYLKNK 550
D+ + E + + ++E + +P GV+FYL++K
Sbjct: 511 TAQDNPKSATEQSGTGIRSESETESEASEITIPPSDPAVPQVPVEGEDYGKGVIFYLRDK 570
Query: 551 EIVGVLLWNVDDDIIIESDFPSPLRINVARKILAQHPKIQSNTDLIKL 694
+VG++LWNV + R+ +ARKI+ + + ++ KL
Sbjct: 571 VVVGIVLWNVFN------------RMPIARKIIKDGEQHEDLNEVAKL 606
>sp|Q9Z0X1|PDCD8_MOUSE Programmed cell death protein 8, mitochondrial precursor
(Apoptosis-inducing factor)
Length = 612
Score = 86.7 bits (213), Expect = 7e-17
Identities = 69/228 (30%), Positives = 98/228 (42%), Gaps = 19/228 (8%)
Frame = +2
Query: 68 FLVNAELLAREGIYVAGDSCSFFDVNLGRRRVEHWNHSEESGSIAGHNMVAYVLKSVDAS 247
F VNAEL AR I+VAGD+ F+D+ LGRRRVEH +H+ SG +AG NM
Sbjct: 420 FRVNAELQARSNIWVAGDAACFYDIKLGRRRVEHHDHAVVSGRLAGENMTGAA------- 472
Query: 248 YFDSNSPKFVDNSNLIYDIFKPRIYNFQSSFAFKFNIYNSLEAIGLTDSKLET-KTFVAT 424
KP Y QS F EAIGL DS L T F
Sbjct: 473 --------------------KP--YWHQSMFWSDLGPDVGYEAIGLVDSSLPTVGVFAKA 510
Query: 425 DFYDDDFNIVESDEKVVEKALKPKTEHDPLDMP------------------GVVFYLKNK 550
D+ + E + + ++E + +P GV+FYL++K
Sbjct: 511 TAQDNPKSATEQSGTGIRSESETESEASEITIPPSAPAVPQVPVEGEDYGKGVIFYLRDK 570
Query: 551 EIVGVLLWNVDDDIIIESDFPSPLRINVARKILAQHPKIQSNTDLIKL 694
+VG++LWNV + R+ +ARKI+ + + ++ KL
Sbjct: 571 VVVGIVLWNVFN------------RMPIARKIIKDGEQHEDLNEVAKL 606
>sp|O95831|PDCD8_HUMAN Programmed cell death protein 8, mitochondrial precursor
(Apoptosis-inducing factor)
Length = 613
Score = 86.3 bits (212), Expect = 9e-17
Identities = 68/228 (29%), Positives = 98/228 (42%), Gaps = 19/228 (8%)
Frame = +2
Query: 68 FLVNAELLAREGIYVAGDSCSFFDVNLGRRRVEHWNHSEESGSIAGHNMVAYVLKSVDAS 247
F VNAEL AR I+VAGD+ F+D+ LGRRRVEH +H+ SG +AG NM
Sbjct: 421 FRVNAELQARSNIWVAGDAACFYDIKLGRRRVEHHDHAVVSGRLAGENMTGAA------- 473
Query: 248 YFDSNSPKFVDNSNLIYDIFKPRIYNFQSSFAFKFNIYNSLEAIGLTDSKLET-KTFVAT 424
KP Y QS F EAIGL DS L T F
Sbjct: 474 --------------------KP--YWHQSMFWSDLGPDVGYEAIGLVDSSLPTVGVFAKA 511
Query: 425 DFYDDDFNIVESDEKVVEKALKPKTEHDPLDMP------------------GVVFYLKNK 550
D+ + E + + ++E + +P GV+FYL++K
Sbjct: 512 TAQDNPKSATEQSGTGIRSESETESEASEITIPPSTPAVPQAPVQGEDYGKGVIFYLRDK 571
Query: 551 EIVGVLLWNVDDDIIIESDFPSPLRINVARKILAQHPKIQSNTDLIKL 694
+VG++LWN+ + R+ +ARKI+ + + ++ KL
Sbjct: 572 VVVGIVLWNIFN------------RMPIARKIIKDGEQHEDLNEVAKL 607
>sp|Q9VQ79|PDCD8_DROME Putative oxidoreductase CG7263, mitochondrial precursor
Length = 739
Score = 86.3 bits (212), Expect = 9e-17
Identities = 72/229 (31%), Positives = 95/229 (41%), Gaps = 20/229 (8%)
Frame = +2
Query: 68 FLVNAELLAREGIYVAGDSCSFFDVNLGRRRVEHWNHSEESGSIAGHNMVAYVLKSVDAS 247
F+VNAEL AR +YVAGD+ FFD LGRRRVEH +HS SG +AG NM
Sbjct: 547 FVVNAELEARRNLYVAGDASCFFDPLLGRRRVEHHDHSVVSGRLAGENMTG--------- 597
Query: 248 YFDSNSPKFVDNSNLIYDIFKPRIYNFQSSFAFKFNIYNSLEAIGLTDSKLETKTFVA-- 421
+ Y QS F E IGL DS L T A
Sbjct: 598 --------------------AKKPYQHQSMFWSDLGPEIGYEGIGLVDSSLPTVGVFALP 637
Query: 422 TDFYDDDFNIVESDEKVVEKALKPKTEHDPLDM------------------PGVVFYLKN 547
++ + ES + V + ++ D GV+FYLKN
Sbjct: 638 SESATRVDQLSESSDSDVPETSTSSSQSSKSDAGASQDGVTCDPDEAGNYGKGVIFYLKN 697
Query: 548 KEIVGVLLWNVDDDIIIESDFPSPLRINVARKILAQHPKIQSNTDLIKL 694
+IVG+LLWN+ + RI +AR I+ Q+ K ++ KL
Sbjct: 698 DKIVGILLWNLFN------------RIGLARTIINQNKKYDDLNEVAKL 734
>sp|O51597|SECF_BORBU Protein-export membrane protein secF
Length = 299
Score = 33.1 bits (74), Expect = 0.95
Identities = 43/159 (27%), Positives = 66/159 (41%), Gaps = 12/159 (7%)
Frame = +2
Query: 290 LIYDIFKPRIYNFQSSFAFKFNIYNSLEAIGLTDSKLET------KTFVATDFYDDDFN- 448
LIY F YN+ F+ + NI S+E + +++++ KT + D N
Sbjct: 26 LIYTFFYHGGYNWGIDFSSRVNINLSIEKSNIKENEIKKIFSPIYKTLDVNSIFSPDQNK 85
Query: 449 -----IVESDEKVVEKALKPKTEHDPLDMPGVVFYLKNKEIVGVLLWNVDDDIIIESDFP 613
+V+SD V++ A K + + LD K KE + V D I+S F
Sbjct: 86 SEFSIMVKSD--VIDYAFKTEVQKTILD--------KLKETFDANI-EVLDSYFIDSSFS 134
Query: 614 SPLRINVARKILAQHPKIQSNTDLIKLASEFDLSFYVNS 730
S LRI +L I LI + F LS+ + S
Sbjct: 135 STLRIRSIFLVLGTFILI-----LIYITLRFKLSYAIAS 168
>sp|Q9WTV1|CH3L1_RAT Chitinase-3-like protein 1 precursor (Cartilage glycoprotein 39)
(GP-39)
Length = 381
Score = 31.6 bits (70), Expect = 2.8
Identities = 13/28 (46%), Positives = 18/28 (64%)
Frame = +2
Query: 506 DPLDMPGVVFYLKNKEIVGVLLWNVDDD 589
DP + V YLKNK++ G ++W VD D
Sbjct: 328 DPESVKNKVKYLKNKQLAGAMVWAVDLD 355
>sp|P58145|ROAA_ASTLO Ribosomal operon-associated A protein (RoaA)
Length = 519
Score = 30.4 bits (67), Expect = 6.2
Identities = 16/55 (29%), Positives = 27/55 (49%)
Frame = +2
Query: 284 SNLIYDIFKPRIYNFQSSFAFKFNIYNSLEAIGLTDSKLETKTFVATDFYDDDFN 448
+NL+ + P + +F F FN Y +L I L K+ K F+ ++ +FN
Sbjct: 126 NNLVKFVMYPFLELKTENFVFNFNFYTNLYDIFLNFKKILLKNFMKLYYFTINFN 180
>sp|Q02137|ILVB_LACLA Acetolactate synthase large subunit (AHAS) (Acetohydroxy-acid
synthase large subunit) (ALS)
Length = 575
Score = 30.4 bits (67), Expect = 6.2
Identities = 23/88 (26%), Positives = 43/88 (48%)
Frame = +2
Query: 329 QSSFAFKFNIYNSLEAIGLTDSKLETKTFVATDFYDDDFNIVESDEKVVEKALKPKTEHD 508
QS F + N EA G+ KL+ +A DD I+ DE ++ + L K+EH
Sbjct: 493 QSVFDVEPNFQLLAEAYGIKHVKLDNPKTLA-----DDLKIITEDEPMLIEVLISKSEH- 546
Query: 509 PLDMPGVVFYLKNKEIVGVLLWNVDDDI 592
+P + L + E++G+ + ++++
Sbjct: 547 --VLPMIPAGLHSDEMIGLHFTDENEEV 572
>sp|Q9WZH8|SYC_THEMA Cysteinyl-tRNA synthetase (Cysteine--tRNA ligase) (CysRS)
Length = 460
Score = 30.0 bits (66), Expect = 8.1
Identities = 30/107 (28%), Positives = 47/107 (43%), Gaps = 6/107 (5%)
Frame = +2
Query: 359 YNSLEAIGLTDSKLETKTFVATDFYDDDFNIVESDEKVVEKALKPKTEHDPLDMPGVVFY 538
+ A+G+ + +T TDF +D IVE EK+VEK +TE GV F
Sbjct: 96 WRDAHALGIRPANFHPRT---TDFVED---IVEIIEKLVEKGFAYQTE------TGVYFD 143
Query: 539 LKNKEIVGVLLWNVDDDII------IESDFPSPLRINVARKILAQHP 661
++ E G L +D+I ++ SPL ++ +K P
Sbjct: 144 VRKFEKYGELSKKKIEDLIAGARVEVDETKKSPLDFSLWKKAKPGEP 190
>sp|Q7VD64|TAL_PROMA Transaldolase
Length = 335
Score = 30.0 bits (66), Expect = 8.1
Identities = 25/92 (27%), Positives = 45/92 (48%), Gaps = 2/92 (2%)
Frame = +2
Query: 440 DFNIVESDEKVVEKALKPKTEHDPLDMPGVVFYLKNKEIVGVLLWNVDDDIIIESDFPSP 619
D ++ S ++V A + + LD V F KEI+ ++ V ++ F +
Sbjct: 53 DKSLQASRQRVGSFASAKEVVDEALDEVCVTF---GKEILKIIPGRVSTEVDARLSFNTQ 109
Query: 620 LRINVARKILAQHPKIQSNTD--LIKLASEFD 709
IN ARKI+ + K+ + + LIK+AS ++
Sbjct: 110 ATINKARKIIDLYKKVDISKERVLIKVASTWE 141
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 83,077,498
Number of Sequences: 369166
Number of extensions: 1544153
Number of successful extensions: 4593
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4299
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4591
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 7956112725
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)