Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_008_E03
(292 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P30543|AA2AR_RAT Adenosine A2a receptor 29 2.9
sp|P00723|BGAL_KLULA Beta-galactosidase (Lactase) 28 6.5
sp|Q39688|EP1G_DAUCA Epidermis-specific secreted glycoprote... 28 6.5
sp|P97829|CD47_RAT Leukocyte surface antigen CD47 precursor... 28 6.5
sp|Q9ES74|NEK7_MOUSE Serine/threonine-protein kinase Nek7 (... 28 8.5
sp|Q8TDX7|NEK7_HUMAN Serine/threonine-protein kinase Nek7 (... 28 8.5
sp|Q60613|AA2AR_MOUSE Adenosine A2a receptor 28 8.5
>sp|P30543|AA2AR_RAT Adenosine A2a receptor
Length = 410
Score = 29.3 bits (64), Expect = 2.9
Identities = 20/78 (25%), Positives = 35/78 (44%)
Frame = +2
Query: 5 EQSENVLSEEVKARVWTRCADGQFRLCYGSVCYICSYTFWSYWSRTCEPRDFKCWR*VNM 184
E++ + L +EV A G F LC+ + I +TF+ R P W M
Sbjct: 214 ERTRSTLQKEVHAAKSLAIIVGLFALCWLPLHIINCFTFFCSTCRHAPP-----WL---M 265
Query: 185 HYCIVIDHVEKVLNVYVF 238
+ I++ H V+N +++
Sbjct: 266 YLAIILSHSNSVVNPFIY 283
>sp|P00723|BGAL_KLULA Beta-galactosidase (Lactase)
Length = 1025
Score = 28.1 bits (61), Expect = 6.5
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = +2
Query: 110 SYTFWSYWSRTCEPRDFKCWR*VNM 184
S TFW + EPRDFK W+ N+
Sbjct: 785 SITFWRPPTNNDEPRDFKNWKKYNI 809
>sp|Q39688|EP1G_DAUCA Epidermis-specific secreted glycoprotein EP1 precursor (52/54-kDa
medium protein)
Length = 389
Score = 28.1 bits (61), Expect = 6.5
Identities = 18/57 (31%), Positives = 25/57 (43%), Gaps = 2/57 (3%)
Frame = +2
Query: 26 SEEVKARVWTRCADGQFRLCYGSVCYICSYTFWSY--WSRTCEPRDFKCWR*VNMHY 190
SE + ++ +C G F LC S C C + WS+TCEP + HY
Sbjct: 326 SESSECQLPKKC--GNFGLCEESQCVGCPTSSGPVLAWSKTCEPPKLSSCGPKDFHY 380
>sp|P97829|CD47_RAT Leukocyte surface antigen CD47 precursor (Integrin-associated
protein) (IAP)
Length = 303
Score = 28.1 bits (61), Expect = 6.5
Identities = 19/67 (28%), Positives = 35/67 (52%), Gaps = 5/67 (7%)
Frame = +3
Query: 96 CAISAHTHFGLTGVELVNPETS--NVGDKLTCII---VSLSIMWKKY*MCTFSMKSFC*S 260
C ++ + G T +EL N S + +K+ +I +++ + W K+ + T KS +
Sbjct: 112 CEVTELSREGKTVIELKNRPVSWFSTNEKILIVIFPILAILLFWGKFGILTLKYKSSHTN 171
Query: 261 KRIIISL 281
KRII+ L
Sbjct: 172 KRIILLL 178
>sp|Q9ES74|NEK7_MOUSE Serine/threonine-protein kinase Nek7 (NimA-related protein kinase
7)
Length = 302
Score = 27.7 bits (60), Expect = 8.5
Identities = 12/38 (31%), Positives = 21/38 (55%)
Frame = -3
Query: 173 IANI*SLGVHKFYSSKTKMCMSRYSTRYHSTNEIVHRH 60
+ + LG+ +F+SSKT S T Y+ + E +H +
Sbjct: 174 VVKLGDLGLGRFFSSKTTAAHSLVGTPYYMSPERIHEN 211
>sp|Q8TDX7|NEK7_HUMAN Serine/threonine-protein kinase Nek7 (NimA-related protein kinase
7)
Length = 302
Score = 27.7 bits (60), Expect = 8.5
Identities = 12/38 (31%), Positives = 21/38 (55%)
Frame = -3
Query: 173 IANI*SLGVHKFYSSKTKMCMSRYSTRYHSTNEIVHRH 60
+ + LG+ +F+SSKT S T Y+ + E +H +
Sbjct: 174 VVKLGDLGLGRFFSSKTTAAHSLVGTPYYMSPERIHEN 211
>sp|Q60613|AA2AR_MOUSE Adenosine A2a receptor
Length = 410
Score = 27.7 bits (60), Expect = 8.5
Identities = 19/78 (24%), Positives = 35/78 (44%)
Frame = +2
Query: 5 EQSENVLSEEVKARVWTRCADGQFRLCYGSVCYICSYTFWSYWSRTCEPRDFKCWR*VNM 184
E++ + L +EV A G F LC+ + I +TF+ + P W M
Sbjct: 214 ERTRSTLQKEVHAAKSLAIIVGLFALCWLPLHIINCFTFFCSTCQHAPP-----WL---M 265
Query: 185 HYCIVIDHVEKVLNVYVF 238
+ I++ H V+N +++
Sbjct: 266 YLAIILSHSNSVVNPFIY 283
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 32,037,065
Number of Sequences: 369166
Number of extensions: 587130
Number of successful extensions: 1594
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 1567
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1594
length of database: 68,354,980
effective HSP length: 66
effective length of database: 56,162,470
effective search space used: 1684874100
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)