Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_008_D22
(863 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q8CST4|IF2_STAES Translation initiation factor IF-2 >gi|... 40 0.011
sp|P25339|PUF4_YEAST PUF4 protein 39 0.019
sp|P38691|KSP1_YEAST Serine/threonine-protein kinase KSP1 39 0.024
sp|Q17060|MRJP3_APIME Major royal jelly protein 3 precursor... 38 0.032
sp|Q8WXA9|SFR12_HUMAN Splicing factor, arginine/serine-rich... 37 0.093
sp|Q8JFN7|ACH91_ONCMY Neuronal acetylcholine receptor prote... 37 0.093
sp|P36168|YK76_YEAST Hypothetical 137.5 kDa protein in MPL1... 36 0.12
sp|P40467|YIN0_YEAST Putative 108.8 kDa transcriptional reg... 36 0.16
sp|P04931|ARP_PLAFA Asparagine-rich protein (AG319) (ARP) 35 0.35
sp|P40070|LSM4_YEAST U6 snRNA-associated Sm-like protein LSm4 34 0.46
>sp|Q8CST4|IF2_STAES Translation initiation factor IF-2
sp|Q5HPS2|IF2_STAEQ Translation initiation factor IF-2
Length = 720
Score = 39.7 bits (91), Expect = 0.011
Identities = 23/86 (26%), Positives = 43/86 (50%)
Frame = +1
Query: 7 ENKYRTSRYKSNNKRDRSSNHYYQPSNHGNYNHNRKKNDMKDNEDYERPSKKNSYEKRKP 186
+ K++ S+ K NK++ +NH +K N+ +++ D E+ KN+ + K
Sbjct: 47 DKKFKASQAKDTNKQNTQNNH-------------QKSNNKQNSNDKEKQQSKNNSKPTKK 93
Query: 187 VKQNNYYGDSQMDNKKPAEIKRQSKN 264
+QNN G Q N K + ++ +KN
Sbjct: 94 KEQNN-KGKQQNKNNKTNKNQKNNKN 118
>sp|P25339|PUF4_YEAST PUF4 protein
Length = 888
Score = 38.9 bits (89), Expect = 0.019
Identities = 20/78 (25%), Positives = 37/78 (47%), Gaps = 4/78 (5%)
Frame = +1
Query: 4 HENKYRTSRYKSNNKRDRSSN----HYYQPSNHGNYNHNRKKNDMKDNEDYERPSKKNSY 171
H + NNK SN + S HG YN+N N+ +N + + NS
Sbjct: 485 HNDNDNIGNSNYNNKDTGRSNVGKMKNMKNSYHGYYNNNNNNNNNNNNNNNSNATNSNSA 544
Query: 172 EKRKPVKQNNYYGDSQMD 225
EK++ +++++ + D+ +D
Sbjct: 545 EKQRKIEESSRFADAVLD 562
>sp|P38691|KSP1_YEAST Serine/threonine-protein kinase KSP1
Length = 1029
Score = 38.5 bits (88), Expect = 0.024
Identities = 19/72 (26%), Positives = 36/72 (50%)
Frame = +1
Query: 7 ENKYRTSRYKSNNKRDRSSNHYYQPSNHGNYNHNRKKNDMKDNEDYERPSKKNSYEKRKP 186
+++Y +NNK+ R + +Y +N+ N NH N+ + Y + KN + R+P
Sbjct: 531 QHRYMEGFSNNNNKQYRQNRNYNNNNNNSNNNHGSNYNNFNNGNSYIKGWNKNFNKYRRP 590
Query: 187 VKQNNYYGDSQM 222
++Y G S +
Sbjct: 591 -SSSSYTGKSPL 601
Score = 30.8 bits (68), Expect = 5.1
Identities = 21/68 (30%), Positives = 36/68 (52%), Gaps = 2/68 (2%)
Frame = +1
Query: 4 HENKYRTSRYKSNNKRDRSSNHYYQPSNHGNYNHNRK--KNDMKDNEDYERPSKKNSYEK 177
+ +YR +R NN + S+N++ SN+ N+N+ K K+ Y RPS +SY
Sbjct: 542 NNKQYRQNR-NYNNNNNNSNNNH--GSNYNNFNNGNSYIKGWNKNFNKYRRPS-SSSYTG 597
Query: 178 RKPVKQNN 201
+ P+ + N
Sbjct: 598 KSPLSRYN 605
>sp|Q17060|MRJP3_APIME Major royal jelly protein 3 precursor (MRJP-3) (Bee-milk protein)
(Royal jelly protein RJP57-1)
Length = 544
Score = 38.1 bits (87), Expect = 0.032
Identities = 21/79 (26%), Positives = 40/79 (50%), Gaps = 2/79 (2%)
Frame = +1
Query: 1 KHENKYRTSRYKSNNKRDRSSNHYYQPSN--HGNYNHNRKKNDMKDNEDYERPSKKNSYE 174
+++N+ ++ N + D N Q N +GN + K+ND K N + + +K+N
Sbjct: 460 QNDNRQNDNKQNGNRQNDNKQNGNRQNDNKQNGNRQNGNKQNDNKQNGNRQNDNKRNG-- 517
Query: 175 KRKPVKQNNYYGDSQMDNK 231
R+ QNN +++ DN+
Sbjct: 518 NRQNDNQNNQNDNNRNDNQ 536
Score = 34.7 bits (78), Expect = 0.35
Identities = 22/88 (25%), Positives = 42/88 (47%)
Frame = +1
Query: 1 KHENKYRTSRYKSNNKRDRSSNHYYQPSNHGNYNHNRKKNDMKDNEDYERPSKKNSYEKR 180
++ NK +R N + D N GN ++ K+N + N++ + +++N K+
Sbjct: 450 QNANKQNGNRQNDNRQNDNKQN--------GNRQNDNKQNGNRQNDNKQNGNRQNG-NKQ 500
Query: 181 KPVKQNNYYGDSQMDNKKPAEIKRQSKN 264
KQN G+ Q DNK+ + ++N
Sbjct: 501 NDNKQN---GNRQNDNKRNGNRQNDNQN 525
Score = 32.0 bits (71), Expect = 2.3
Identities = 18/77 (23%), Positives = 40/77 (51%), Gaps = 2/77 (2%)
Frame = +1
Query: 37 SNNKRDRSSNHYYQPSNHGNYNHNRKKNDMKDNEDYERPSKKNSYEKRKPVKQ--NNYYG 210
+ N ++++++ +GN ++ ++ND K N + + +K+N ++ KQ N G
Sbjct: 439 NQNANNQNADNQNANKQNGNRQNDNRQNDNKQNGNRQNDNKQNG-NRQNDNKQNGNRQNG 497
Query: 211 DSQMDNKKPAEIKRQSK 261
+ Q DNK+ + +K
Sbjct: 498 NKQNDNKQNGNRQNDNK 514
>sp|Q8WXA9|SFR12_HUMAN Splicing factor, arginine/serine-rich 12 (Serine-arginine-rich
splicing regulatory protein 86) (SRrp86) (Splicing
regulatory protein 508) (SRrp508)
Length = 508
Score = 36.6 bits (83), Expect = 0.093
Identities = 23/87 (26%), Positives = 41/87 (47%), Gaps = 1/87 (1%)
Frame = +1
Query: 4 HENKYRTSRYKSNNKRDRSSNHYYQPSNHGNYNHNRKKNDMKDNEDYERPSKKNSYEK-R 180
H S KS+++R RS S H + +HNR ++ KD + P KK S + R
Sbjct: 195 HTRSKSRSSSKSHSRRKRSQ------SKHRSRSHNRSRSRQKDRRRSKSPHKKRSKSRER 248
Query: 181 KPVKQNNYYGDSQMDNKKPAEIKRQSK 261
+ + ++ D + D ++ + K + K
Sbjct: 249 RKSRSRSHSRDKRKDTREKIKEKERVK 275
>sp|Q8JFN7|ACH91_ONCMY Neuronal acetylcholine receptor protein, alpha-9-I subunit
precursor (Nicotinic acetylcholine receptor subunit
alpha 9-I) (NACHR alpha 9-I)
Length = 572
Score = 36.6 bits (83), Expect = 0.093
Identities = 22/78 (28%), Positives = 36/78 (46%)
Frame = +1
Query: 4 HENKYRTSRYKSNNKRDRSSNHYYQPSNHGNYNHNRKKNDMKDNEDYERPSKKNSYEKRK 183
H+N RY + RD +HY SN N NH+ ++ E E EKR+
Sbjct: 414 HQNHLDNCRYANGGHRD---DHY---SNRSNQNHHSNRSQTSKGEGGE--------EKRE 459
Query: 184 PVKQNNYYGDSQMDNKKP 237
P++ ++ G ++D + P
Sbjct: 460 PLRHYHHIGREELDYQAP 477
>sp|P36168|YK76_YEAST Hypothetical 137.5 kDa protein in MPL1-PPC1 intergenic region
Length = 1195
Score = 36.2 bits (82), Expect = 0.12
Identities = 20/73 (27%), Positives = 36/73 (49%)
Frame = +1
Query: 34 KSNNKRDRSSNHYYQPSNHGNYNHNRKKNDMKDNEDYERPSKKNSYEKRKPVKQNNYYGD 213
K N+ RD+ N+ +N+ N++ N N+ +N+D + NS + +N +
Sbjct: 200 KDNSARDQDFNNSGNNNNNNNHSSNNNDNNNNNNDDNNNNNNSNSRDNNNNSDDSNEREE 259
Query: 214 SQMDNKKPAEIKR 252
+ D+ KPA KR
Sbjct: 260 N--DSCKPASNKR 270
>sp|P40467|YIN0_YEAST Putative 108.8 kDa transcriptional regulatory protein in FKH1-STH1
intergenic region
Length = 964
Score = 35.8 bits (81), Expect = 0.16
Identities = 19/84 (22%), Positives = 38/84 (45%)
Frame = +1
Query: 4 HENKYRTSRYKSNNKRDRSSNHYYQPSNHGNYNHNRKKNDMKDNEDYERPSKKNSYEKRK 183
+ N + +NN + ++N +N+ N N+N+ ND D PS + + +
Sbjct: 849 NNNDNNNNNNNNNNNNNNNNNSGNSSNNNNNNNNNKNNNDFGIKIDNNSPSYEGFPQLQI 908
Query: 184 PVKQNNYYGDSQMDNKKPAEIKRQ 255
P+ Q+N + + + EIK +
Sbjct: 909 PLSQDNLNIEDKEEMSPNIEIKNE 932
Score = 35.4 bits (80), Expect = 0.21
Identities = 22/80 (27%), Positives = 37/80 (46%), Gaps = 3/80 (3%)
Frame = +1
Query: 10 NKYRTSRYKSNNKR---DRSSNHYYQPSNHGNYNHNRKKNDMKDNEDYERPSKKNSYEKR 180
N Y K+NN + S+N++ +N N N+N N+ +N + S N+
Sbjct: 823 NNYNNVSGKNNNNNNITNNSNNNHNNNNNDNNNNNNNNNNNNNNNNNSGNSSNNNNNNNN 882
Query: 181 KPVKQNNYYGDSQMDNKKPA 240
K NN +G ++DN P+
Sbjct: 883 N--KNNNDFG-IKIDNNSPS 899
Score = 30.8 bits (68), Expect = 5.1
Identities = 20/88 (22%), Positives = 34/88 (38%), Gaps = 10/88 (11%)
Frame = +1
Query: 25 SRYKSNNKRDRSSNHYYQPS----------NHGNYNHNRKKNDMKDNEDYERPSKKNSYE 174
S +N+ D +N+Y S N+ N NHN ND +N + + N+
Sbjct: 810 SNASNNSNPDTINNNYNNVSGKNNNNNNITNNSNNNHNNNNNDNNNNNNNNNNNNNNNNN 869
Query: 175 KRKPVKQNNYYGDSQMDNKKPAEIKRQS 258
NN +++ +N +I S
Sbjct: 870 SGNSSNNNNNNNNNKNNNDFGIKIDNNS 897
>sp|P04931|ARP_PLAFA Asparagine-rich protein (AG319) (ARP)
Length = 537
Score = 34.7 bits (78), Expect = 0.35
Identities = 21/89 (23%), Positives = 40/89 (44%), Gaps = 3/89 (3%)
Frame = +1
Query: 7 ENKYRTSRYKSNNKR---DRSSNHYYQPSNHGNYNHNRKKNDMKDNEDYERPSKKNSYEK 177
+NK+ T+ NN + + + N N+ N N+N N + N ++ NS
Sbjct: 165 KNKFYTNYMNKNNLKFTQNNNDNMNINEDNNNNNNNNNNNNGVFSNYQNNNMNRNNSINI 224
Query: 178 RKPVKQNNYYGDSQMDNKKPAEIKRQSKN 264
++ + NN ++ NK ++ K Q+ N
Sbjct: 225 KRNLNNNNNINNNM--NKMGSQDKNQNSN 251
Score = 30.8 bits (68), Expect = 5.1
Identities = 18/81 (22%), Positives = 35/81 (43%), Gaps = 5/81 (6%)
Frame = +1
Query: 37 SNNKRDRSSNHYYQPSNHGNYNHNRK-----KNDMKDNEDYERPSKKNSYEKRKPVKQNN 201
+NN + ++N+ SN+ N N NR K ++ +N + K + + NN
Sbjct: 194 NNNNNNNNNNNNGVFSNYQNNNMNRNNSINIKRNLNNNNNINNNMNKMGSQDKNQNSNNN 253
Query: 202 YYGDSQMDNKKPAEIKRQSKN 264
+Y + N+K + + N
Sbjct: 254 FYMNYNYQNRKNSMNNNMNNN 274
>sp|P40070|LSM4_YEAST U6 snRNA-associated Sm-like protein LSm4
Length = 187
Score = 34.3 bits (77), Expect = 0.46
Identities = 18/65 (27%), Positives = 28/65 (43%)
Frame = +1
Query: 34 KSNNKRDRSSNHYYQPSNHGNYNHNRKKNDMKDNEDYERPSKKNSYEKRKPVKQNNYYGD 213
K + + ++N H Y +NR N+ + N Y R + N R+P QN Y +
Sbjct: 88 KVKQQINSNNNSNSNGPGHKRYYNNRDSNNNRGN--YNRRNNNNGNSNRRPYSQNRQYNN 145
Query: 214 SQMDN 228
S N
Sbjct: 146 SNSSN 150
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.305 0.123 0.359
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 30,819,796
Number of Sequences: 369166
Number of extensions: 481215
Number of successful extensions: 2181
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1490
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1872
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8582957970
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 43 (22.0 bits)