Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_008_C08
(772 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q29554|ECHA_PIG Trifunctional enzyme alpha subunit, mito... 214 3e-55
sp|P40939|ECHA_HUMAN Trifunctional enzyme alpha subunit, mi... 211 1e-54
sp|Q64428|ECHA_RAT Trifunctional enzyme alpha subunit, mito... 207 2e-53
sp|P77399|FADJ_ECOLI Fatty acid oxidation complex alpha sub... 102 9e-22
sp|Q8X8I2|FADB_ECO57 Fatty oxidation complex alpha subunit ... 88 3e-17
sp|Q9L6L5|FADB_SALTY Fatty oxidation complex alpha subunit ... 87 5e-17
sp|P21177|FADB_ECOLI Fatty oxidation complex alpha subunit ... 86 9e-17
sp|Q8ZAN0|FADB_YERPE Fatty oxidation complex alpha subunit ... 84 4e-16
sp|Q8Z3C6|FADB_SALTI Fatty oxidation complex alpha subunit ... 82 2e-15
sp|P28793|FAOB_PSEFR Fatty oxidation complex alpha subunit ... 80 6e-15
>sp|Q29554|ECHA_PIG Trifunctional enzyme alpha subunit, mitochondrial precursor
(TP-alpha) (78 kDa gastrin-binding protein) [Includes:
Long-chain enoyl-CoA hydratase ; Long chain
3-hydroxyacyl-CoA dehydrogenase ]
Length = 763
Score = 214 bits (544), Expect = 3e-55
Identities = 114/235 (48%), Positives = 148/235 (62%), Gaps = 4/235 (1%)
Frame = +3
Query: 3 VGLRQGKVVIVVADGPGFYTTRILSTMMTEAIRLLQEGVTPSELDKVTRDFGWPVGSASL 182
VGL+QGKV+IVV DGPGFYTTR L+ MM+E +R+LQEGV P +LD +T FG+PVG+A+L
Sbjct: 528 VGLKQGKVIIVVKDGPGFYTTRCLAPMMSEVLRILQEGVGPKKLDSLTTSFGFPVGAATL 587
Query: 183 ADEVGIDVAFHVGQELELALGNRMKGGNVDILKDMVAAXXXXXXXXXXXXXXXXXXXIEN 362
DEVG+DVA HV + L G R GGN+D+LK M++ N
Sbjct: 588 MDEVGMDVAKHVAENLGKIFGERFAGGNLDVLKQMISKGFLGRKSGKGFYVYQEGVKNRN 647
Query: 363 ----TECLDILKKYYVKPPTDNFAERIQYRLFSRFVNEAVLCLQDGILFDPVDGDIGAVY 530
TE + K +P + E IQYRL +RFVNEAVLCLQ+GIL P +GDIGAV+
Sbjct: 648 VNSDTESILASLKIPSRPDISS-DEDIQYRLVTRFVNEAVLCLQEGILATPAEGDIGAVF 706
Query: 531 XXXXXXXXXXXXRYLDNHGAEKIVKLMEHYSSQYGEEFKPCQLLIDHAKDPSKKF 695
R++D +GA+K+V + Y + YG++F PCQLLIDHA P+KKF
Sbjct: 707 GLGFPPCLGGPFRFVDLYGAQKVVDRLRKYEAIYGKQFTPCQLLIDHASSPNKKF 761
>sp|P40939|ECHA_HUMAN Trifunctional enzyme alpha subunit, mitochondrial precursor
(TP-alpha) (78 kDa gastrin-binding protein) [Includes:
Long-chain enoyl-CoA hydratase ; Long chain
3-hydroxyacyl-CoA dehydrogenase ]
Length = 763
Score = 211 bits (538), Expect = 1e-54
Identities = 111/235 (47%), Positives = 151/235 (64%), Gaps = 3/235 (1%)
Frame = +3
Query: 3 VGLRQGKVVIVVADGPGFYTTRILSTMMTEAIRLLQEGVTPSELDKVTRDFGWPVGSASL 182
VGL+QGKV+IVV DGPGFYTTR L+ MM+E IR+LQEGV P +LD +T FG+PVG+A+L
Sbjct: 528 VGLKQGKVIIVVKDGPGFYTTRCLAPMMSEVIRILQEGVDPKKLDSLTTSFGFPVGAATL 587
Query: 183 ADEVGIDVAFHVGQELELALGNRMKGGNVDILKDMVAAXXXXXXXXXXXXXXXXXXXIE- 359
DEVG+DVA HV ++L G R GGN ++L MV+ +
Sbjct: 588 VDEVGVDVAKHVAEDLGKVFGERFGGGNPELLTQMVSKGFLGRKSGKGFYIYQEGVKRKD 647
Query: 360 -NTECLDILKKYYVKPPTD-NFAERIQYRLFSRFVNEAVLCLQDGILFDPVDGDIGAVYX 533
N++ IL + P ++ + E IQ+RL +RFVNEAV+CLQ+GIL P +GDIGAV+
Sbjct: 648 LNSDMDSILASLKLPPKSEVSSDEDIQFRLVTRFVNEAVMCLQEGILATPAEGDIGAVFG 707
Query: 534 XXXXXXXXXXXRYLDNHGAEKIVKLMEHYSSQYGEEFKPCQLLIDHAKDPSKKFH 698
R++D +GA+KIV ++ Y + YG++F PCQLL DHA P+KKF+
Sbjct: 708 LGFPPCLGGPFRFVDLYGAQKIVDRLKKYEAAYGKQFTPCQLLADHANSPNKKFY 762
>sp|Q64428|ECHA_RAT Trifunctional enzyme alpha subunit, mitochondrial precursor
(TP-alpha) [Includes: Long-chain enoyl-CoA hydratase ;
Long chain 3-hydroxyacyl-CoA dehydrogenase ]
Length = 763
Score = 207 bits (528), Expect = 2e-53
Identities = 116/235 (49%), Positives = 151/235 (64%), Gaps = 3/235 (1%)
Frame = +3
Query: 3 VGLRQGKVVIVVADGPGFYTTRILSTMMTEAIRLLQEGVTPSELDKVTRDFGWPVGSASL 182
VGL+QGKV+IVV DGPGFYTTR L+ MM+E IR+LQEGV P +LD +T FG+PVG+A+L
Sbjct: 528 VGLKQGKVIIVVKDGPGFYTTRCLAPMMSEVIRILQEGVDPKKLDALTTGFGFPVGAATL 587
Query: 183 ADEVGIDVAFHVGQELELALGNRMKGGNVDILKDMVAAXXXXXXXXXXXXXXXXXXXIE- 359
ADEVGIDVA HV ++L A G R GG+V++LK MV+ +
Sbjct: 588 ADEVGIDVAQHVAEDLGKAFGERFGGGSVELLKLMVSKGFLGRKSGKGFYIYQSGSKNKN 647
Query: 360 -NTECLDILKKYYV-KPPTDNFAERIQYRLFSRFVNEAVLCLQDGILFDPVDGDIGAVYX 533
N+E +IL + P + E IQYR+ +RFVNEAVLCLQ+GIL P +GDIGAV+
Sbjct: 648 LNSEIDNILVNLRLPAKPEVSSDEDIQYRVITRFVNEAVLCLQEGILATPEEGDIGAVFG 707
Query: 534 XXXXXXXXXXXRYLDNHGAEKIVKLMEHYSSQYGEEFKPCQLLIDHAKDPSKKFH 698
R++D +GA+K+V + Y S YG +F PCQLL D A + SKKF+
Sbjct: 708 LGFPPCLGGPFRFVDLYGAQKVVDRLRKYESAYGTQFTPCQLLRDLANNSSKKFY 762
>sp|P77399|FADJ_ECOLI Fatty acid oxidation complex alpha subunit [Includes: Enoyl-CoA
hydratase ; 3-hydroxyacyl-CoA dehydrogenase ;
3-hydroxybutyryl-CoA epimerase ]
Length = 714
Score = 102 bits (255), Expect = 9e-22
Identities = 70/222 (31%), Positives = 101/222 (45%), Gaps = 3/222 (1%)
Frame = +3
Query: 12 RQGKVVIVVADGPGFYTTRILSTMMTEAIRLLQEGVTPSELDKVTRDFGWPVGSASLADE 191
+QGK IVV D GFY RIL+ + EAIR+L +G +D FG+PVG L DE
Sbjct: 479 KQGKTPIVVRDKAGFYVNRILAPYINEAIRMLTQGERVEHIDAALVKFGFPVGPIQLLDE 538
Query: 192 VGIDVAFHVGQELELALGNRMKG-GNV--DILKDMVAAXXXXXXXXXXXXXXXXXXXIEN 362
VGID + LE A G R NV IL D +
Sbjct: 539 VGIDTGTKIIPVLEAAYGERFSAPANVVSSILNDDRKGRKNGRGFYLYGQKGRKSKKQVD 598
Query: 363 TECLDILKKYYVKPPTDNFAERIQYRLFSRFVNEAVLCLQDGILFDPVDGDIGAVYXXXX 542
++ + A ++ R +NEAV C+ + ++ DGDIGAV+
Sbjct: 599 PAIYPLIG---TQGQGRISAPQVAERCVMLMLNEAVRCVDEQVIRSVRDGDIGAVFGIGF 655
Query: 543 XXXXXXXXRYLDNHGAEKIVKLMEHYSSQYGEEFKPCQLLID 668
RY+D+ GA ++V +M+ ++QYG F PC+ L++
Sbjct: 656 PPFLGGPFRYIDSLGAGEVVAIMQRLATQYGSRFTPCERLVE 697
>sp|Q8X8I2|FADB_ECO57 Fatty oxidation complex alpha subunit [Includes: Enoyl-CoA hydratase
; Delta(3)-cis-delta(2)-trans-enoyl-CoA isomerase ;
3-hydroxyacyl-CoA dehydrogenase ; 3-hydroxybutyryl-CoA
epimerase ]
Length = 729
Score = 87.8 bits (216), Expect = 3e-17
Identities = 61/208 (29%), Positives = 88/208 (42%), Gaps = 5/208 (2%)
Frame = +3
Query: 12 RQGKVVIVVADGPGFYTTRILSTMMTEAIRLLQEGVTPSELDKVT-RDFGWPVGSASLAD 188
+ GK IVV D PGF+ R+L +LL++G ++DKV + FGWP+G A L D
Sbjct: 483 KMGKTPIVVNDCPGFFVNRVLFPYFAGFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLD 542
Query: 189 EVGIDVAFHVGQELELALGNRMKGGNVDILKDMVAAXXXXXXXXXXXXXXXXXXX----I 356
VGID A H + RM+ D + + A
Sbjct: 543 VVGIDTAHHAQAVMAAGFPQRMQKDYRDAIDALFDANRFGQKNGLGFWHYKEDSKGKPKK 602
Query: 357 ENTECLDILKKYYVKPPTDNFAERIQYRLFSRFVNEAVLCLQDGILFDPVDGDIGAVYXX 536
E +D L +P D E I R+ VNE V CL++GI+ P + D+ VY
Sbjct: 603 EEDAAVDDLLAEVSQPKRDFSEEEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGL 662
Query: 537 XXXXXXXXXXRYLDNHGAEKIVKLMEHY 620
R+LD G+ K + + + Y
Sbjct: 663 GFPPFHGGAFRWLDTLGSAKYLDMAQQY 690
>sp|Q9L6L5|FADB_SALTY Fatty oxidation complex alpha subunit [Includes: Enoyl-CoA hydratase
; Delta(3)-cis-delta(2)-trans-enoyl-CoA isomerase ;
3-hydroxyacyl-CoA dehydrogenase ; 3-hydroxybutyryl-CoA
epimerase ]
Length = 729
Score = 87.0 bits (214), Expect = 5e-17
Identities = 59/208 (28%), Positives = 88/208 (42%), Gaps = 5/208 (2%)
Frame = +3
Query: 12 RQGKVVIVVADGPGFYTTRILSTMMTEAIRLLQEGVTPSELDKVT-RDFGWPVGSASLAD 188
+ GK IVV D PGF+ R+L +LL++G ++DKV + FGWP+G A L D
Sbjct: 483 KMGKTPIVVNDCPGFFVNRVLFPYFAGFSQLLRDGADFRKVDKVMEKQFGWPMGPAYLLD 542
Query: 189 EVGIDVAFHVGQELELALGNRMKGGNVDILKDMVAAXXXXXXXXXXXXXXXXXXX----I 356
VGID A H + RM+ D + + A
Sbjct: 543 VVGIDTAHHAQAVMAAGFPQRMQKDYRDAIDALFDASRFGQKNGLGFWRYKEDSKGKPKK 602
Query: 357 ENTECLDILKKYYVKPPTDNFAERIQYRLFSRFVNEAVLCLQDGILFDPVDGDIGAVYXX 536
E +D L +P D + I R+ +NE V CL++GI+ P + D+ VY
Sbjct: 603 EEDAAVDDLLASVSQPKRDFSDDEIIARMMIPMINEVVRCLEEGIIASPAEADMALVYGL 662
Query: 537 XXXXXXXXXXRYLDNHGAEKIVKLMEHY 620
R+LD G+ K + + + Y
Sbjct: 663 GFPPFHGGAFRWLDTQGSAKYLDMAQQY 690
>sp|P21177|FADB_ECOLI Fatty oxidation complex alpha subunit [Includes: Enoyl-CoA hydratase
; Delta(3)-cis-delta(2)-trans-enoyl-CoA isomerase ;
3-hydroxyacyl-CoA dehydrogenase ; 3-hydroxybutyryl-CoA
epimerase ]
Length = 729
Score = 86.3 bits (212), Expect = 9e-17
Identities = 60/208 (28%), Positives = 88/208 (42%), Gaps = 5/208 (2%)
Frame = +3
Query: 12 RQGKVVIVVADGPGFYTTRILSTMMTEAIRLLQEGVTPSELDKVT-RDFGWPVGSASLAD 188
+ GK IVV D PGF+ R+L +LL++G ++DKV + FGWP+G A L D
Sbjct: 483 KMGKTPIVVNDCPGFFVNRVLFPYFAGFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLD 542
Query: 189 EVGIDVAFHVGQELELALGNRMKGGNVDILKDMVAA----XXXXXXXXXXXXXXXXXXXI 356
VGID A H + RM+ D + + A
Sbjct: 543 VVGIDTAHHAQAVMAAGFPQRMQKDYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKK 602
Query: 357 ENTECLDILKKYYVKPPTDNFAERIQYRLFSRFVNEAVLCLQDGILFDPVDGDIGAVYXX 536
E ++ L +P D E I R+ VNE V CL++GI+ P + D+ VY
Sbjct: 603 EEDAAVEDLLAEVSQPKRDFSEEEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGL 662
Query: 537 XXXXXXXXXXRYLDNHGAEKIVKLMEHY 620
R+LD G+ K + + + Y
Sbjct: 663 GFPPFHGGAFRWLDTLGSAKYLDMAQQY 690
>sp|Q8ZAN0|FADB_YERPE Fatty oxidation complex alpha subunit [Includes: Enoyl-CoA hydratase
; Delta(3)-cis-delta(2)-trans-enoyl-CoA isomerase ;
3-hydroxyacyl-CoA dehydrogenase ; 3-hydroxybutyryl-CoA
epimerase ]
Length = 729
Score = 84.0 bits (206), Expect = 4e-16
Identities = 62/209 (29%), Positives = 90/209 (43%), Gaps = 5/209 (2%)
Frame = +3
Query: 12 RQGKVVIVVADGPGFYTTRILSTMMTEAIRLLQEGVTPSELDKVT-RDFGWPVGSASLAD 188
+ GK IVV D PGF+ R+L + L+++G ++DKV + FGWP+G A L D
Sbjct: 483 QMGKTPIVVNDCPGFFVNRVLFPYLAGFGMLVRDGGDFHQIDKVMEKQFGWPMGPAYLLD 542
Query: 189 EVGIDVAFHVGQELELALGNRMK---GGNVDILKD-MVAAXXXXXXXXXXXXXXXXXXXI 356
VGID A H + RM VD++ D
Sbjct: 543 VVGIDTAHHAQAVMAAGFPERMNKDYRDAVDVMFDNQRFGQKNGQGFYRYTQDAKGKPRK 602
Query: 357 ENTECLDILKKYYVKPPTDNFAERIQYRLFSRFVNEAVLCLQDGILFDPVDGDIGAVYXX 536
EN E +D L +P + E I R +NE V CL++GI+ +GD+ VY
Sbjct: 603 ENDEQVDKLLAEISQPLQEFSDEDIIARTMIPMINEVVRCLEEGIIASAAEGDMALVYGL 662
Query: 537 XXXXXXXXXXRYLDNHGAEKIVKLMEHYS 623
RYLD G+ V++ + Y+
Sbjct: 663 GFPPFHGGVFRYLDTLGSANYVEMAQRYA 691
>sp|Q8Z3C6|FADB_SALTI Fatty oxidation complex alpha subunit [Includes: Enoyl-CoA hydratase
; Delta(3)-cis-delta(2)-trans-enoyl-CoA isomerase ;
3-hydroxyacyl-CoA dehydrogenase ; 3-hydroxybutyryl-CoA
epimerase ]
Length = 729
Score = 81.6 bits (200), Expect = 2e-15
Identities = 57/208 (27%), Positives = 87/208 (41%), Gaps = 5/208 (2%)
Frame = +3
Query: 12 RQGKVVIVVADGPGFYTTRILSTMMTEAIRLLQEGVTPSELDKVT-RDFGWPVGSASLAD 188
+ GK IVV + PGF+ R+L +LL++G ++DKV + FGWP+G A L D
Sbjct: 483 KMGKTPIVVNNCPGFFVNRVLFPYFAGFSQLLRDGADFRKVDKVMEKQFGWPMGPAYLLD 542
Query: 189 EVGIDVAFHVGQELELALGNRMKGGNVDILKDMVAA----XXXXXXXXXXXXXXXXXXXI 356
VGID A H + RM+ D + + A
Sbjct: 543 VVGIDTAHHAQAVMAAGFPQRMQKEYRDAIDALFDASRFGQKNGLGFWRYKEDSKGKPKK 602
Query: 357 ENTECLDILKKYYVKPPTDNFAERIQYRLFSRFVNEAVLCLQDGILFDPVDGDIGAVYXX 536
E +D L + D + I R+ +NE V CL++GI+ P + D+ VY
Sbjct: 603 EEDAAVDDLLASVSQTKRDFSDDEIIARMMIPMINEVVRCLEEGIIASPAEADMALVYGL 662
Query: 537 XXXXXXXXXXRYLDNHGAEKIVKLMEHY 620
R+LD G+ K + + + Y
Sbjct: 663 GFPPFHGGAFRWLDTQGSAKYLDMAQQY 690
>sp|P28793|FAOB_PSEFR Fatty oxidation complex alpha subunit [Includes: Enoyl-CoA hydratase
; Delta(3)-cis-delta(2)-trans-enoyl-CoA isomerase ;
3-hydroxyacyl-CoA dehydrogenase ; 3-hydroxybutyryl-CoA
epimerase ]
Length = 715
Score = 80.1 bits (196), Expect = 6e-15
Identities = 67/235 (28%), Positives = 98/235 (41%), Gaps = 7/235 (2%)
Frame = +3
Query: 12 RQGKVVIVVADGPGFYTTRILSTMMTEAIRLLQEGVTPSELDKVTRDFGWPVGSASLADE 191
+ GK IVV D PGF R+L +L+ GV +DKV FGWP+G A L D
Sbjct: 484 KMGKNPIVVNDCPGFLVNRVLFPYFGGFAKLVSAGVDFVRIDKVMEKFGWPMGPAYLMDV 543
Query: 192 VGIDVAFHVGQELELALGNRMKGGN---VDILKD--MVAAXXXXXXXXXXXXXXXXXXXI 356
VGID H + +RMK +D L + + +
Sbjct: 544 VGIDTGHHGRDVMAEGFPDRMKDDRRSAIDALYEAKRLGQKNGKGFYAYEADKKGKQKKL 603
Query: 357 ENTECLDILKK--YYVKPPTDNFAERIQYRLFSRFVNEAVLCLQDGILFDPVDGDIGAVY 530
++ L++LK Y + TD E I + E V CL+DGI+ + D+G VY
Sbjct: 604 VDSSVLEVLKPIVYEQRDVTD---EDIINWMMIPLCLETVRCLEDGIVETAAEADMGLVY 660
Query: 531 XXXXXXXXXXXXRYLDNHGAEKIVKLMEHYSSQYGEEFKPCQLLIDHAKDPSKKF 695
RY+D+ G + V L + Y ++ G + P L + AK+ F
Sbjct: 661 GIGFPLFRGGALRYIDSIGVAEFVALADQY-AELGALYHPTAKLREMAKNGQSFF 714
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 80,651,999
Number of Sequences: 369166
Number of extensions: 1531156
Number of successful extensions: 4199
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4066
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4170
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 7163732800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)