Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_007_P23
(894 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9UI08|EVL_HUMAN Ena/vasodilator-stimulated phosphoprote... 48 3e-05
sp|P70429|EVL_MOUSE Ena/vasodilator-stimulated phosphoprote... 46 1e-04
sp|P50551|VASP_CANFA Vasodilator-stimulated phosphoprotein ... 42 0.003
sp|P70460|VASP_MOUSE Vasodilator-stimulated phosphoprotein ... 38 0.033
sp|O97902|DDEF1_BOVIN 130-kDa phosphatidylinositol 4,5-biph... 37 0.074
sp|P50552|VASP_HUMAN Vasodilator-stimulated phosphoprotein ... 37 0.097
sp|O07650|TONB_CAMCO Protein tonB 36 0.13
sp|Q60554|NKX61_MESAU Homeobox protein Nkx-6.1 35 0.22
sp|Q99MA9|NKX61_MOUSE Homeobox protein Nkx-6.1 35 0.22
sp|P46582|YLB8_CAEEL Hypothetical protein C34E10.8 in chrom... 35 0.22
>sp|Q9UI08|EVL_HUMAN Ena/vasodilator-stimulated phosphoprotein-like protein
(Ena/VASP-like protein)
Length = 416
Score = 48.1 bits (113), Expect = 3e-05
Identities = 36/141 (25%), Positives = 60/141 (42%), Gaps = 4/141 (2%)
Frame = +2
Query: 356 RAKQETMKHTSTNSNADNISRESPTPPVQSTQSISSKPPKMDFLSEIKLKTQNRQDGINP 535
R K + D E P+ +S+PP SE K R + +
Sbjct: 278 RRKAASQSDKPAEKKEDESQMEDPSTSPSPGTRAASQPPNS---SEAGRKPWERSNSVEK 334
Query: 536 PKKIEQPRTPAI---PTVNLPFKN-PNQSSKPLPSNQDPTTASFVTSADLEKFRAELLQD 703
P RTP++ P P ++ P+ KP S D +F DL++ + E+L++
Sbjct: 335 PVSSILSRTPSVAKSPEAKSPLQSQPHSRMKPAGSVNDMALDAF----DLDRMKQEILEE 390
Query: 704 IVSNMEKFKEDIINGIISKLN 766
+V + K KE+II+ I +L+
Sbjct: 391 VVRELHKVKEEIIDAIRQELS 411
>sp|P70429|EVL_MOUSE Ena/vasodilator-stimulated phosphoprotein-like protein
(Ena/VASP-like protein)
Length = 414
Score = 46.2 bits (108), Expect = 1e-04
Identities = 38/146 (26%), Positives = 66/146 (45%), Gaps = 4/146 (2%)
Frame = +2
Query: 341 LASQLRAKQETMKHTSTNSNADNISRESPTPPVQSTQSISSKPPKMDFLSEIKLKTQNRQ 520
LA + +A +T K + D E P+ +S+PP SE K R
Sbjct: 273 LAKRRKAASQTDK--PADRKEDESQTEDPSTSPSPGTRATSQPPNS---SEAGRKPWERS 327
Query: 521 DGINPPKKIEQPRTPAI---PTVNLPFKN-PNQSSKPLPSNQDPTTASFVTSADLEKFRA 688
+ + P RTP++ P P ++ P+ KP S D + + DL++ +
Sbjct: 328 NSVEKPVSSLLSRTPSVAKSPEAKSPLQSQPHSRVKPAGSVNDVG----LDALDLDRMKQ 383
Query: 689 ELLQDIVSNMEKFKEDIINGIISKLN 766
E+L+++V + K KE+II+ I +L+
Sbjct: 384 EILEEVVRELHKVKEEIIDAIRQELS 409
>sp|P50551|VASP_CANFA Vasodilator-stimulated phosphoprotein (VASP)
Length = 384
Score = 41.6 bits (96), Expect = 0.003
Identities = 35/138 (25%), Positives = 60/138 (43%), Gaps = 15/138 (10%)
Frame = +2
Query: 395 SNADNISRESPTPPVQSTQSISSKPPKMDFLSEIKLKTQNRQDGINPPKKIEQPRTPA-- 568
S + S P P +ST+S + + + + + + Q G PPK P
Sbjct: 242 SKQEEASGGPPVPKAESTRSTGGGLME-EMNAMLARRRKATQVGEKPPKDESANEEPEAR 300
Query: 569 --IP----TVNLPFKNPN-------QSSKPLPSNQDPTTASFVTSADLEKFRAELLQDIV 709
+P TV P++ + SS S P+T S +DLE+ + ELL+++
Sbjct: 301 VPVPAQSETVRRPWEKNSTTLPRMKSSSSVTTSEAHPSTPSSSDESDLERVKQELLEEVR 360
Query: 710 SNMEKFKEDIINGIISKL 763
++K KE+II + +L
Sbjct: 361 KELQKVKEEIIEAFVQEL 378
>sp|P70460|VASP_MOUSE Vasodilator-stimulated phosphoprotein (VASP)
Length = 376
Score = 38.1 bits (87), Expect = 0.033
Identities = 27/107 (25%), Positives = 49/107 (45%), Gaps = 4/107 (3%)
Frame = +2
Query: 455 ISSKPPKMDFLS----EIKLKTQNRQDGINPPKKIEQPRTPAIPTVNLPFKNPNQSSKPL 622
+ KPPK + S E +L Q+ P ++ + + +P + SS
Sbjct: 276 VGEKPPKDESASQEESEARLPAQSE-----PVRRPWEKNSTTLPRMK-------SSSSVT 323
Query: 623 PSNQDPTTASFVTSADLEKFRAELLQDIVSNMEKFKEDIINGIISKL 763
S P+T +DLE+ + ELL+++ ++K KE+II + +L
Sbjct: 324 TSEAHPSTPCSSDDSDLERVKQELLEEVRKELQKMKEEIIEVFVQEL 370
>sp|O97902|DDEF1_BOVIN 130-kDa phosphatidylinositol 4,5-biphosphate-dependent ARF1
GTPase-activating protein (PIP2-dependent ARF1 GAP)
(ADP-ribosylation factor-directed GTPase-activating
protein 1) (ARF GTPase-activating protein 1) (Development
and differentiation-enhancing factor 1)
(Differentiation-enhancing factor 1) (DEF-1)
Length = 1129
Score = 37.0 bits (84), Expect = 0.074
Identities = 23/94 (24%), Positives = 41/94 (43%)
Frame = +2
Query: 383 TSTNSNADNISRESPTPPVQSTQSISSKPPKMDFLSEIKLKTQNRQDGINPPKKIEQPRT 562
TST+S I+ P PP +T+ P + ++ + +PP RT
Sbjct: 781 TSTDSPTSPIAEAPPLPPRNATKGPPGPPSTLPLSTQTSSGSSTLSKKRSPPPPPGHKRT 840
Query: 563 PAIPTVNLPFKNPNQSSKPLPSNQDPTTASFVTS 664
+ P LP PN+ + P ++ P+++S T+
Sbjct: 841 LSDPPSPLPHGPPNKGAVPWGNDVGPSSSSKTTN 874
>sp|P50552|VASP_HUMAN Vasodilator-stimulated phosphoprotein (VASP)
Length = 380
Score = 36.6 bits (83), Expect = 0.097
Identities = 17/52 (32%), Positives = 29/52 (55%)
Frame = +2
Query: 608 SSKPLPSNQDPTTASFVTSADLEKFRAELLQDIVSNMEKFKEDIINGIISKL 763
SS S P T S +DL++ + ELL+++ ++K KE+II + +L
Sbjct: 323 SSSVTTSETQPCTPSSSDYSDLQRVKQELLEEVKKELQKVKEEIIEAFVQEL 374
>sp|O07650|TONB_CAMCO Protein tonB
Length = 232
Score = 36.2 bits (82), Expect = 0.13
Identities = 35/123 (28%), Positives = 56/123 (45%), Gaps = 4/123 (3%)
Frame = +2
Query: 368 ETMKHTSTNSN----ADNISRESPTPPVQSTQSISSKPPKMDFLSEIKLKTQNRQDGINP 535
ET+K N N S PT P Q+ Q S+ PK + + EIK ++ I
Sbjct: 40 ETIKENKFNMAIKHFVQNSSDMKPTQPTQTIQEPSNVQPK-EPVQEIKKIKPRKEKLIAK 98
Query: 536 PKKIEQPRTPAIPTVNLPFKNPNQSSKPLPSNQDPTTASFVTSADLEKFRAELLQDIVSN 715
PKKI P ++ P K+ N +P+ Q P +S+ + A +E L++I S
Sbjct: 99 PKKIIPPAN--AKAISQPKKDTNM-QQPIMQQQTPQASSYQSVALTS--NSEFLKEIKSA 153
Query: 716 MEK 724
+++
Sbjct: 154 IDE 156
>sp|Q60554|NKX61_MESAU Homeobox protein Nkx-6.1
Length = 364
Score = 35.4 bits (80), Expect = 0.22
Identities = 28/99 (28%), Positives = 42/99 (42%), Gaps = 4/99 (4%)
Frame = +2
Query: 383 TSTNSNADNISRESPTPPVQSTQSISSKPPKMDFLSEIKLKTQN----RQDGINPPKKIE 550
T S++ + S SP+PP+ + KPP LS + Q GIN +
Sbjct: 45 TGPPSSSSSSSSSSPSPPLGAHNPGGLKPPAAGGLSSLSSPPQQLSAATPHGIN--DILS 102
Query: 551 QPRTPAIPTVNLPFKNPNQSSKPLPSNQDPTTASFVTSA 667
+P P LP +P+ SS S+ T+AS +A
Sbjct: 103 RPSMPVASGAALPSASPSGSSSSSSSSASATSASAAAAA 141
>sp|Q99MA9|NKX61_MOUSE Homeobox protein Nkx-6.1
Length = 365
Score = 35.4 bits (80), Expect = 0.22
Identities = 28/96 (29%), Positives = 42/96 (43%), Gaps = 4/96 (4%)
Frame = +2
Query: 392 NSNADNISRESPTPPVQSTQSISSKPPKMDFLSEIKLKTQN----RQDGINPPKKIEQPR 559
+S++ + S SP+PP+ S KPP LS + Q GIN + +P
Sbjct: 49 SSSSSSSSSSSPSPPLGSHNPGGLKPPAAGGLSSLGSPPQQLSAATPHGIN--DILSRPS 106
Query: 560 TPAIPTVNLPFKNPNQSSKPLPSNQDPTTASFVTSA 667
P LP +P+ SS S+ T+AS +A
Sbjct: 107 MPVASGAALPSASPSGSSSSSSSSASATSASAAAAA 142
>sp|P46582|YLB8_CAEEL Hypothetical protein C34E10.8 in chromosome III
Length = 1024
Score = 35.4 bits (80), Expect = 0.22
Identities = 29/113 (25%), Positives = 50/113 (44%)
Frame = +2
Query: 326 PAANDLASQLRAKQETMKHTSTNSNADNISRESPTPPVQSTQSISSKPPKMDFLSEIKLK 505
PAA + S R ET+ ST+S A I T S +++S +PP S++
Sbjct: 844 PAAMTITSNKRKIDETLASESTSSEATLI---HDTTSSSSAETVSGEPPAKK-SSDVSAP 899
Query: 506 TQNRQDGINPPKKIEQPRTPAIPTVNLPFKNPNQSSKPLPSNQDPTTASFVTS 664
+ + +KI++P+TP K+ + + P PS + P A+ +
Sbjct: 900 VPSPE---KEKEKIDRPKTP---------KSSTKRTTPTPSGRTPRAAAIAAN 940
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 88,489,400
Number of Sequences: 369166
Number of extensions: 1619776
Number of successful extensions: 5361
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4985
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5323
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 8982382310
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)