Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_007_N14
(801 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P59780|AP3S2_HUMAN Adapter-related protein complex 3 sig... 181 2e-45
sp|Q92572|AP3S1_HUMAN Adapter-related protein complex 3 sig... 177 2e-44
sp|Q09905|YAJ5_SCHPO Adaptin complex small chain homolog C3... 116 6e-26
sp|P47064|AP22_YEAST Probable adaptin complex small chain h... 102 1e-21
sp|P61967|AP1S1_MOUSE Adapter-related protein complex 1 sig... 86 1e-16
sp|P56377|AP1S2_HUMAN Adapter-related protein complex 1 sig... 84 3e-16
sp|Q9Y587|AP4S1_HUMAN Adapter-related protein complex 4 sig... 83 8e-16
sp|Q9DB50|AP1S2_MOUSE Adapter-related protein complex 1 sig... 83 8e-16
sp|Q9WVL1|AP4S1_MOUSE Adapter-related protein complex 4 sig... 82 2e-15
sp|Q96PC3|AP1S3_HUMAN Adapter-related protein complex 1 sig... 81 3e-15
>sp|P59780|AP3S2_HUMAN Adapter-related protein complex 3 sigma 2 subunit (Sigma-adaptin
3b) (AP-3 complex sigma-3B subunit) (Sigma-3B-adaptin)
sp|Q8BSZ2|AP3S2_MOUSE Adapter-related protein complex 3 sigma 2 subunit (Sigma-adaptin
3b) (AP-3 complex sigma-3B subunit) (Sigma-3B-adaptin)
sp|Q5RDP9|AP3S2_PONPY Adapter-related protein complex 3 sigma 2 subunit (Sigma-adaptin
3b) (AP-3 complex sigma-3B subunit) (Sigma-3B-adaptin)
Length = 193
Score = 181 bits (459), Expect = 2e-45
Identities = 88/125 (70%), Positives = 108/125 (86%)
Frame = +3
Query: 318 GKDNKLVYRHYATLYFVFCVDSSESELGILDLIQVFVESLDKAFENVCELDLIFNIDKVH 497
G D KL+YRHYATLYFVFCVDSSESELGILDLIQVFVE+LDK FENVCELDLIF++DKVH
Sbjct: 58 GSDYKLIYRHYATLYFVFCVDSSESELGILDLIQVFVETLDKCFENVCELDLIFHMDKVH 117
Query: 498 HILKEVVLGGLVLDTHMTEILSRFEEQNKLEREENGLSIKSGSAVSAVKSINIPQRIRDI 677
+IL+EVV+GG+VL+T+M EI+++ E QN+LE+ E GLS AVSAVK+IN+P+ R+I
Sbjct: 118 YILQEVVMGGMVLETNMNEIVAQIEAQNRLEKSEGGLSAAPARAVSAVKNINLPEIPRNI 177
Query: 678 KLTDL 692
+ DL
Sbjct: 178 NIGDL 182
Score = 85.1 bits (209), Expect = 2e-16
Identities = 36/53 (67%), Positives = 47/53 (88%)
Frame = +2
Query: 101 MIQAILIFNNHGKPRMIKFYEYYTEEMQQKIVQETFQLVSKRSDDVCNFLESG 259
MIQAIL+FNNHGKPR+++FY+ + EE+QQ+IV+ETF LV KR D++CNFLE G
Sbjct: 1 MIQAILVFNNHGKPRLVRFYQRFPEEIQQQIVRETFHLVLKRDDNICNFLEGG 53
>sp|Q92572|AP3S1_HUMAN Adapter-related protein complex 3 sigma 1 subunit (Sigma-adaptin
3a) (AP-3 complex sigma-3A subunit) (Sigma-3A-adaptin)
(Clathrin-associated/assembly/adapter protein, small 3)
sp|Q9DCR2|AP3S1_MOUSE Adapter-related protein complex 3 sigma 1 subunit (Sigma-adaptin
3a) (AP-3 complex sigma-3A subunit) (Sigma-3A-adaptin)
Length = 193
Score = 177 bits (450), Expect = 2e-44
Identities = 85/125 (68%), Positives = 108/125 (86%)
Frame = +3
Query: 318 GKDNKLVYRHYATLYFVFCVDSSESELGILDLIQVFVESLDKAFENVCELDLIFNIDKVH 497
G DNKL+YRHYATLYFVFCVDSSESELGILDLIQVFVE+LDK FENVCELDLIF++DKVH
Sbjct: 58 GSDNKLIYRHYATLYFVFCVDSSESELGILDLIQVFVETLDKCFENVCELDLIFHVDKVH 117
Query: 498 HILKEVVLGGLVLDTHMTEILSRFEEQNKLEREENGLSIKSGSAVSAVKSINIPQRIRDI 677
+IL E+V+GG+VL+T+M EI+++ + QNKLE+ E GL+ AVSAVK++N+P+ R+I
Sbjct: 118 NILAEMVMGGMVLETNMNEIVTQIDAQNKLEKSEAGLAGAPARAVSAVKNMNLPEIPRNI 177
Query: 678 KLTDL 692
+ D+
Sbjct: 178 NIGDI 182
Score = 84.0 bits (206), Expect = 4e-16
Identities = 37/53 (69%), Positives = 47/53 (88%)
Frame = +2
Query: 101 MIQAILIFNNHGKPRMIKFYEYYTEEMQQKIVQETFQLVSKRSDDVCNFLESG 259
MI+AILIFNNHGKPR+ KFY+ Y+E+ QQ+I++ETF LVSKR ++VCNFLE G
Sbjct: 1 MIKAILIFNNHGKPRLSKFYQPYSEDTQQQIIRETFHLVSKRDENVCNFLEGG 53
>sp|Q09905|YAJ5_SCHPO Adaptin complex small chain homolog C30D11.05
Length = 165
Score = 116 bits (291), Expect = 6e-26
Identities = 55/94 (58%), Positives = 70/94 (74%)
Frame = +3
Query: 327 NKLVYRHYATLYFVFCVDSSESELGILDLIQVFVESLDKAFENVCELDLIFNIDKVHHIL 506
N+++YR YATLYFVF VD ESELGILDLIQVFVE+LD+ F NVCELDL+F ++H IL
Sbjct: 59 NRIIYRQYATLYFVFVVDEGESELGILDLIQVFVEALDRCFNNVCELDLVFKFQEIHAIL 118
Query: 507 KEVVLGGLVLDTHMTEILSRFEEQNKLEREENGL 608
EVV GGLVL+T++ EI+ + Q + N +
Sbjct: 119 AEVVSGGLVLETNLNEIVLAAQNQMPKTKRSNAM 152
Score = 58.9 bits (141), Expect = 2e-08
Identities = 27/52 (51%), Positives = 34/52 (65%)
Frame = +2
Query: 101 MIQAILIFNNHGKPRMIKFYEYYTEEMQQKIVQETFQLVSKRSDDVCNFLES 256
MI A+ IFNN GKPR+ KFY E +QQK++ + + VS R CNFLES
Sbjct: 1 MIYAVFIFNNKGKPRLTKFYTPIDESIQQKLIGDIYAAVSTRPPTACNFLES 52
>sp|P47064|AP22_YEAST Probable adaptin complex small chain homolog
Length = 194
Score = 102 bits (254), Expect = 1e-21
Identities = 48/90 (53%), Positives = 67/90 (74%)
Frame = +3
Query: 315 NGKDNKLVYRHYATLYFVFCVDSSESELGILDLIQVFVESLDKAFENVCELDLIFNIDKV 494
N +D +++Y++YATLYF F VD ESEL ILDLIQ FVESLD+ F V ELDLIFN +
Sbjct: 69 NNEDIQIIYKNYATLYFTFIVDDQESELAILDLIQTFVESLDRCFTEVNELDLIFNWQTL 128
Query: 495 HHILKEVVLGGLVLDTHMTEILSRFEEQNK 584
+L+E+V GG+V++T++ I++ +E NK
Sbjct: 129 ESVLEEIVQGGMVIETNVNRIVASVDELNK 158
Score = 42.0 bits (97), Expect = 0.002
Identities = 17/45 (37%), Positives = 31/45 (68%)
Frame = +2
Query: 101 MIQAILIFNNHGKPRMIKFYEYYTEEMQQKIVQETFQLVSKRSDD 235
MI A+LIFN +PR++KFY Q+ ++++ ++L+S+R+ D
Sbjct: 1 MIHAVLIFNKKCQPRLVKFYTPVDLPKQKLLLEQVYELISQRNSD 45
>sp|P61967|AP1S1_MOUSE Adapter-related protein complex 1 sigma 1A subunit (Sigma-adaptin
1A) (Adaptor protein complex AP-1 sigma-1A subunit)
(Golgi adaptor HA1/AP1 adaptin sigma-1A subunit)
(Clathrin assembly protein complex 1 sigma-1A small
chain) (Clathrin coat assembly protein AP19) (HA1 19 kDa
subunit) (Sigma 1a subunit of AP-1 clathrin)
sp|P61966|AP1S1_HUMAN Adapter-related protein complex 1 sigma 1A subunit (Sigma-adaptin
1A) (Adaptor protein complex AP-1 sigma-1A subunit)
(Golgi adaptor HA1/AP1 adaptin sigma-1A subunit)
(Clathrin assembly protein complex 1 sigma-1A small
chain) (Clathrin coat assembly protein AP19) (HA1 19 kDa
subunit) (Sigma 1a subunit of AP-1 clathrin)
Length = 158
Score = 85.9 bits (211), Expect = 1e-16
Identities = 41/93 (44%), Positives = 62/93 (66%)
Frame = +3
Query: 321 KDNKLVYRHYATLYFVFCVDSSESELGILDLIQVFVESLDKAFENVCELDLIFNIDKVHH 500
+D K+VY+ YA+LYF ++ ++EL L+LI +VE LDK F +VCELD+IFN +K +
Sbjct: 53 RDLKVVYKRYASLYFCCAIEGQDNELITLELIHRYVELLDKYFGSVCELDIIFNFEKAYF 112
Query: 501 ILKEVVLGGLVLDTHMTEILSRFEEQNKLEREE 599
IL E ++GG V DT +L E+ + L+ E+
Sbjct: 113 ILDEFLMGGDVQDTSKKSVLKAIEQADLLQEED 145
Score = 40.0 bits (92), Expect = 0.007
Identities = 18/51 (35%), Positives = 35/51 (68%)
Frame = +2
Query: 101 MIQAILIFNNHGKPRMIKFYEYYTEEMQQKIVQETFQLVSKRSDDVCNFLE 253
M++ +L+F+ GK R+ K+Y +++ ++K+V+E Q+V R +C+FLE
Sbjct: 1 MMRFMLLFSRQGKLRLQKWYLATSDKERKKMVRELMQVVLARKPKMCSFLE 51
>sp|P56377|AP1S2_HUMAN Adapter-related protein complex 1 sigma 1B subunit (Sigma-adaptin
1B) (Adaptor protein complex AP-1 sigma-1B subunit)
(Golgi adaptor HA1/AP1 adaptin sigma-1B subunit)
(Clathrin assembly protein complex 1 sigma-1B small
chain) (Sigma 1B subunit of AP-1 clathrin)
Length = 157
Score = 84.3 bits (207), Expect = 3e-16
Identities = 40/92 (43%), Positives = 61/92 (66%)
Frame = +3
Query: 321 KDNKLVYRHYATLYFVFCVDSSESELGILDLIQVFVESLDKAFENVCELDLIFNIDKVHH 500
+D K+VY+ YA+LYF ++ ++EL L++I +VE LDK F +VCELD+IFN +K +
Sbjct: 52 RDLKIVYKRYASLYFCCAIEDQDNELITLEIIHRYVELLDKYFGSVCELDIIFNFEKAYF 111
Query: 501 ILKEVVLGGLVLDTHMTEILSRFEEQNKLERE 596
IL E +LGG V +T +L E+ + L+ E
Sbjct: 112 ILDEFLLGGEVQETSKKNVLKAIEQADLLQEE 143
Score = 38.5 bits (88), Expect = 0.022
Identities = 18/50 (36%), Positives = 32/50 (64%)
Frame = +2
Query: 104 IQAILIFNNHGKPRMIKFYEYYTEEMQQKIVQETFQLVSKRSDDVCNFLE 253
+Q +L+F+ GK R+ K+Y +++ ++KI +E Q V R +C+FLE
Sbjct: 1 MQFMLLFSRQGKLRLQKWYVPLSDKEKKKITRELVQTVLARKPKMCSFLE 50
>sp|Q9Y587|AP4S1_HUMAN Adapter-related protein complex 4 sigma 1 subunit (Sigma subunit of
AP-4) (AP-4 adapter complex sigma subunit)
Length = 144
Score = 83.2 bits (204), Expect = 8e-16
Identities = 40/81 (49%), Positives = 57/81 (70%)
Frame = +3
Query: 321 KDNKLVYRHYATLYFVFCVDSSESELGILDLIQVFVESLDKAFENVCELDLIFNIDKVHH 500
KD KL+YR YA L+ V V+ +E+E+ I + I FVE LD+ F V ELD++FN+DKVH
Sbjct: 53 KDFKLIYRQYAALFIVVGVNDTENEMAIYEFIHNFVEVLDEYFSRVSELDIMFNLDKVHI 112
Query: 501 ILKEVVLGGLVLDTHMTEILS 563
IL E+VL G +++T+ IL+
Sbjct: 113 ILDEMVLNGCIVETNRARILA 133
Score = 33.9 bits (76), Expect = 0.53
Identities = 15/51 (29%), Positives = 28/51 (54%)
Frame = +2
Query: 101 MIQAILIFNNHGKPRMIKFYEYYTEEMQQKIVQETFQLVSKRSDDVCNFLE 253
MI+ L+ N G+ R+ K+YE+ + + E + RS++ C+F+E
Sbjct: 1 MIKFFLMVNKQGQTRLSKYYEHVDINKRTLLETEVIKSCLSRSNEQCSFIE 51
>sp|Q9DB50|AP1S2_MOUSE Adapter-related protein complex 1 sigma 1B subunit (Sigma-adaptin
1B) (Adaptor protein complex AP-1 sigma-1B subunit)
(Golgi adaptor HA1/AP1 adaptin sigma-1B subunit)
(Clathrin assembly protein complex 1 sigma-1B small
chain) (Sigma 1B subunit of AP-1 clathrin)
Length = 160
Score = 83.2 bits (204), Expect = 8e-16
Identities = 39/92 (42%), Positives = 61/92 (66%)
Frame = +3
Query: 321 KDNKLVYRHYATLYFVFCVDSSESELGILDLIQVFVESLDKAFENVCELDLIFNIDKVHH 500
+D K+VY+ YA+LYF ++ ++EL L++I +VE LDK F +VCELD+IFN +K +
Sbjct: 52 RDLKIVYKRYASLYFCCAIEDQDNELITLEIIHRYVELLDKYFGSVCELDIIFNFEKAYF 111
Query: 501 ILKEVVLGGLVLDTHMTEILSRFEEQNKLERE 596
IL E +LGG V +T +L E+ + L+ +
Sbjct: 112 ILDEFLLGGEVQETSKKNVLKAIEQADLLQED 143
Score = 38.5 bits (88), Expect = 0.022
Identities = 18/50 (36%), Positives = 32/50 (64%)
Frame = +2
Query: 104 IQAILIFNNHGKPRMIKFYEYYTEEMQQKIVQETFQLVSKRSDDVCNFLE 253
+Q +L+F+ GK R+ K+Y +++ ++KI +E Q V R +C+FLE
Sbjct: 1 MQFMLLFSRQGKLRLQKWYVPLSDKEKKKITRELVQTVLARKPKMCSFLE 50
>sp|Q9WVL1|AP4S1_MOUSE Adapter-related protein complex 4 sigma 1 subunit (Sigma subunit of
AP-4) (AP-4 adapter complex sigma subunit)
Length = 144
Score = 81.6 bits (200), Expect = 2e-15
Identities = 40/81 (49%), Positives = 56/81 (69%)
Frame = +3
Query: 321 KDNKLVYRHYATLYFVFCVDSSESELGILDLIQVFVESLDKAFENVCELDLIFNIDKVHH 500
KD KL+YR YA L+ V V+ +E+E+ I + I FVE LD F V ELD++FN+DKVH
Sbjct: 53 KDFKLIYRQYAALFVVVGVNDTENEMAIYEFIHNFVEVLDGYFSRVSELDIMFNLDKVHI 112
Query: 501 ILKEVVLGGLVLDTHMTEILS 563
IL E+VL G +++T+ IL+
Sbjct: 113 ILDEMVLNGCIVETNRARILA 133
Score = 32.7 bits (73), Expect = 1.2
Identities = 15/51 (29%), Positives = 27/51 (52%)
Frame = +2
Query: 101 MIQAILIFNNHGKPRMIKFYEYYTEEMQQKIVQETFQLVSKRSDDVCNFLE 253
MI+ L+ N G+ R+ K+YE+ + + E + RS + C+F+E
Sbjct: 1 MIKFFLMVNKQGQTRLSKYYEHVDINKRALLETEVSKSCLSRSSEQCSFIE 51
>sp|Q96PC3|AP1S3_HUMAN Adapter-related protein complex 1 sigma 1C subunit (Sigma-adaptin
1C) (Adaptor protein complex AP-1 sigma-1C subunit)
(Golgi adaptor HA1/AP1 adaptin sigma-1C subunit)
(Clathrin assembly protein complex 1 sigma-1C small
chain) (Sigma 1C subunit of AP-1 clathrin)
Length = 154
Score = 81.3 bits (199), Expect = 3e-15
Identities = 37/90 (41%), Positives = 60/90 (66%)
Frame = +3
Query: 321 KDNKLVYRHYATLYFVFCVDSSESELGILDLIQVFVESLDKAFENVCELDLIFNIDKVHH 500
K+ KLVY+ YA+LYF +++ ++EL L+++ +VE LDK F NVCELD+IFN +K +
Sbjct: 53 KELKLVYKRYASLYFCCAIENQDNELLTLEIVHRYVELLDKYFGNVCELDIIFNFEKAYF 112
Query: 501 ILKEVVLGGLVLDTHMTEILSRFEEQNKLE 590
IL E ++GG + +T + E+ + L+
Sbjct: 113 ILDEFIIGGEIQETSKKIAVKAIEDSDMLQ 142
Score = 34.3 bits (77), Expect = 0.41
Identities = 16/51 (31%), Positives = 30/51 (58%)
Frame = +2
Query: 101 MIQAILIFNNHGKPRMIKFYEYYTEEMQQKIVQETFQLVSKRSDDVCNFLE 253
MI IL+F+ GK R+ K+Y ++ ++KI +E Q++ R +F++
Sbjct: 1 MIHFILLFSRQGKLRLQKWYITLPDKERKKITREIVQIILSRGHRTSSFVD 51
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 68,875,757
Number of Sequences: 369166
Number of extensions: 1139455
Number of successful extensions: 3330
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3223
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3325
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 7570361805
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)