Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_007_M09-2
(426 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P20929|NEBU_HUMAN Nebulin 84 1e-16
sp|Q14847|LASP1_HUMAN LIM and SH3 domain protein 1 (LASP-1)... 81 1e-15
sp|Q61792|LASP1_MOUSE LIM and SH3 domain protein 1 (LASP-1)... 81 1e-15
sp|Q99MZ8|LASP1_RAT LIM and SH3 domain protein 1 (LASP-1) 81 1e-15
sp|Q8I7C3|LAS1_DROME LIM and SH3 domain protein Lasp 79 5e-15
sp|O76041|NEBL_HUMAN Nebulette (Actin-binding Z-disk protein) 77 2e-14
sp|P34416|LASP1_CAEEL LIM and SH3 domain protein F42H10.3 77 2e-14
sp|P38479|ABP1_SACEX Actin-binding protein 57 2e-08
sp|Q9BX66|SRBS1_HUMAN Sorbin and SH3 domain-containing prot... 56 4e-08
sp|P42522|MYOC_DICDI Myosin IC heavy chain 55 6e-08
>sp|P20929|NEBU_HUMAN Nebulin
Length = 6669
Score = 84.0 bits (206), Expect = 1e-16
Identities = 39/72 (54%), Positives = 49/72 (68%)
Frame = +2
Query: 29 QSPVQRVPSQVEPEKVKYKCLYEYMATDNDEVSFSEGDIIINGEVIDQGWMTGTVERTGE 208
Q+ V +PS ++ +Y+YMA D DEVSF +GD IIN + ID+GWM GTV+RTG
Sbjct: 6598 QTTVSSIPSHPSTAGKIFRAMYDYMAADADEVSFKDGDAIINVQAIDEGWMYGTVQRTGR 6657
Query: 209 HGMLPFNYVEKI 244
GMLP NYVE I
Sbjct: 6658 TGMLPANYVEAI 6669
>sp|Q14847|LASP1_HUMAN LIM and SH3 domain protein 1 (LASP-1) (MLN 50)
Length = 261
Score = 80.9 bits (198), Expect = 1e-15
Identities = 39/73 (53%), Positives = 48/73 (65%)
Frame = +2
Query: 26 PQSPVQRVPSQVEPEKVKYKCLYEYMATDNDEVSFSEGDIIINGEVIDQGWMTGTVERTG 205
P +PV S +Y+ +Y+Y A D DEVSF +GD I+N + ID GWM GTVERTG
Sbjct: 189 PAAPVSIQRSAPGGGGKRYRAVYDYSAADEDEVSFQDGDTIVNVQQIDDGWMYGTVERTG 248
Query: 206 EHGMLPFNYVEKI 244
+ GMLP NYVE I
Sbjct: 249 DTGMLPANYVEAI 261
>sp|Q61792|LASP1_MOUSE LIM and SH3 domain protein 1 (LASP-1) (MLN 50)
Length = 263
Score = 80.9 bits (198), Expect = 1e-15
Identities = 39/73 (53%), Positives = 48/73 (65%)
Frame = +2
Query: 26 PQSPVQRVPSQVEPEKVKYKCLYEYMATDNDEVSFSEGDIIINGEVIDQGWMTGTVERTG 205
P +PV S +Y+ +Y+Y A D DEVSF +GD I+N + ID GWM GTVERTG
Sbjct: 191 PAAPVSIQRSAPGGGGKRYRAVYDYSAADEDEVSFQDGDTIVNVQQIDDGWMYGTVERTG 250
Query: 206 EHGMLPFNYVEKI 244
+ GMLP NYVE I
Sbjct: 251 DTGMLPANYVEAI 263
>sp|Q99MZ8|LASP1_RAT LIM and SH3 domain protein 1 (LASP-1)
Length = 263
Score = 80.9 bits (198), Expect = 1e-15
Identities = 39/73 (53%), Positives = 48/73 (65%)
Frame = +2
Query: 26 PQSPVQRVPSQVEPEKVKYKCLYEYMATDNDEVSFSEGDIIINGEVIDQGWMTGTVERTG 205
P +PV S +Y+ +Y+Y A D DEVSF +GD I+N + ID GWM GTVERTG
Sbjct: 191 PAAPVSIQRSAPGGGGKRYRAVYDYSAADEDEVSFQDGDTIVNVQQIDDGWMYGTVERTG 250
Query: 206 EHGMLPFNYVEKI 244
+ GMLP NYVE I
Sbjct: 251 DTGMLPANYVEAI 263
>sp|Q8I7C3|LAS1_DROME LIM and SH3 domain protein Lasp
Length = 657
Score = 78.6 bits (192), Expect = 5e-15
Identities = 40/76 (52%), Positives = 48/76 (63%)
Frame = +2
Query: 14 RQVLPQSPVQRVPSQVEPEKVKYKCLYEYMATDNDEVSFSEGDIIINGEVIDQGWMTGTV 193
RQ S +Q S+ Y+ +Y+Y A D DEVSF EGD+I E ID GWMTG V
Sbjct: 579 RQQPSYSSLQEKQSRQSTAMRVYRAIYDYEAQDVDEVSFREGDVIFEVESIDSGWMTGRV 638
Query: 194 ERTGEHGMLPFNYVEK 241
ERTG+ GMLP NYVE+
Sbjct: 639 ERTGKTGMLPANYVEQ 654
>sp|O76041|NEBL_HUMAN Nebulette (Actin-binding Z-disk protein)
Length = 1014
Score = 76.6 bits (187), Expect = 2e-14
Identities = 34/61 (55%), Positives = 42/61 (68%)
Frame = +2
Query: 56 QVEPEKVKYKCLYEYMATDNDEVSFSEGDIIINGEVIDQGWMTGTVERTGEHGMLPFNYV 235
Q P Y+ +Y+Y A D DEVSF +GD I+N + ID GWM GTV+RTG GMLP NY+
Sbjct: 951 QHSPNLRTYRAMYDYSAQDEDEVSFRDGDYIVNVQPIDDGWMYGTVQRTGRTGMLPANYI 1010
Query: 236 E 238
E
Sbjct: 1011 E 1011
>sp|P34416|LASP1_CAEEL LIM and SH3 domain protein F42H10.3
Length = 335
Score = 76.6 bits (187), Expect = 2e-14
Identities = 34/52 (65%), Positives = 41/52 (78%)
Frame = +2
Query: 83 KCLYEYMATDNDEVSFSEGDIIINGEVIDQGWMTGTVERTGEHGMLPFNYVE 238
K +Y+Y A D DE+SF EGDII+N E ID GWMTGTV+RT + GMLP NYV+
Sbjct: 272 KAIYDYAAADKDEISFLEGDIIVNCEKIDDGWMTGTVQRTLQWGMLPANYVQ 323
>sp|P38479|ABP1_SACEX Actin-binding protein
Length = 617
Score = 56.6 bits (135), Expect = 2e-08
Identities = 30/77 (38%), Positives = 43/77 (55%), Gaps = 2/77 (2%)
Frame = +2
Query: 11 ARQVLPQSPVQRVPSQVEP--EKVKYKCLYEYMATDNDEVSFSEGDIIINGEVIDQGWMT 184
+R +P P QR EP E Y+Y A +++E++F+E D IIN E +D W
Sbjct: 537 SRGSVPPPPPQRAVEPEEPAAEAPWATAEYDYEAGEDNELTFAENDKIINIEFVDDDWWL 596
Query: 185 GTVERTGEHGMLPFNYV 235
G +E TG+ G+ P NYV
Sbjct: 597 GELETTGQKGLFPSNYV 613
>sp|Q9BX66|SRBS1_HUMAN Sorbin and SH3 domain-containing protein 1 (Ponsin) (c-Cbl-associated
protein) (CAP) (SH3 domain protein 5) (SH3P12)
Length = 1292
Score = 55.8 bits (133), Expect = 4e-08
Identities = 28/76 (36%), Positives = 41/76 (53%), Gaps = 3/76 (3%)
Frame = +2
Query: 26 PQSPVQRVP---SQVEPEKVKYKCLYEYMATDNDEVSFSEGDIIINGEVIDQGWMTGTVE 196
PQ+ +RV SQ + Y+ LY Y+ ++DE+ +GDI+ E D GW GT
Sbjct: 1215 PQAQQRRVTPDRSQTSQDLFSYQALYSYIPQNDDELELRDGDIVDVMEKCDDGWFVGTSR 1274
Query: 197 RTGEHGMLPFNYVEKI 244
RT + G P NYV+ +
Sbjct: 1275 RTKQFGTFPGNYVKPL 1290
Score = 33.9 bits (76), Expect = 0.15
Identities = 19/62 (30%), Positives = 31/62 (50%)
Frame = +2
Query: 53 SQVEPEKVKYKCLYEYMATDNDEVSFSEGDIIINGEVIDQGWMTGTVERTGEHGMLPFNY 232
S++ P + K+ ++ A E+ +GDI+ + IDQ W G E G G+ P Y
Sbjct: 793 SEMRPARAKF----DFKAQTLKELPLQKGDIVYIYKQIDQNWYEG--EHHGRVGIFPRTY 846
Query: 233 VE 238
+E
Sbjct: 847 IE 848
Score = 33.1 bits (74), Expect = 0.25
Identities = 14/42 (33%), Positives = 22/42 (52%)
Frame = +2
Query: 119 EVSFSEGDIIINGEVIDQGWMTGTVERTGEHGMLPFNYVEKI 244
E+SF +G+ I +D+ W G + T G+ P YV+ I
Sbjct: 885 EMSFRKGERITLLRQVDENWYEGRIPGTSRQGIFPITYVDVI 926
>sp|P42522|MYOC_DICDI Myosin IC heavy chain
Length = 1181
Score = 55.1 bits (131), Expect = 6e-08
Identities = 29/73 (39%), Positives = 42/73 (57%)
Frame = +2
Query: 26 PQSPVQRVPSQVEPEKVKYKCLYEYMATDNDEVSFSEGDIIINGEVIDQGWMTGTVERTG 205
PQ + P+ P++ Y LYEY A DE++F E D+I + +D W G + RT
Sbjct: 1111 PQPTPMKKPAAPPPQQ--YIALYEYDAMQPDELTFKENDVINLIKKVDADWWQGELVRTK 1168
Query: 206 EHGMLPFNYVEKI 244
+ GMLP NYV++I
Sbjct: 1169 QIGMLPSNYVQQI 1181
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 45,420,616
Number of Sequences: 369166
Number of extensions: 803294
Number of successful extensions: 2195
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2086
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2167
length of database: 68,354,980
effective HSP length: 99
effective length of database: 50,066,215
effective search space used: 2102781030
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)