Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= Dr_sW_007_K24 (589 letters) Database: Non-redundant SwissProt sequences 184,735 sequences; 68,354,980 total letters Score E Sequences producing significant alignments: (bits) Value sp|P33761|DPO3B_BORBU DNA polymerase III, beta chain 30 3.4 sp|P47382|SYK_MYCGE Lysyl-tRNA synthetase (Lysine--tRNA lig... 29 7.6 sp|P41001|TOP2_PLAFK DNA topoisomerase 2 (DNA topoisomerase... 29 9.9
>sp|P33761|DPO3B_BORBU DNA polymerase III, beta chain Length = 385 Score = 30.4 bits (67), Expect = 3.4 Identities = 14/32 (43%), Positives = 21/32 (65%) Frame = +2 Query: 29 NYQDDKAFLERYLKKESTVSKPSIKDRIARLN 124 NY D K+ + + K +S VS +KDR+AR+N Sbjct: 256 NYPDYKSIIPKEQKNKSLVSLGILKDRLARVN 287
>sp|P47382|SYK_MYCGE Lysyl-tRNA synthetase (Lysine--tRNA ligase) (LysRS) Length = 490 Score = 29.3 bits (64), Expect = 7.6 Identities = 16/46 (34%), Positives = 24/46 (52%) Frame = +2 Query: 197 NWVGQDQSAVLSGK*DERIQISSHESRMQS*IELFSPFYCRNYLIE 334 N V DQSA+L + ++++ H+ Q I LF +C LIE Sbjct: 327 NSVKDDQSAILLAE-KHHVKLAKHQQNKQHIINLFFEQFCEQTLIE 371
>sp|P41001|TOP2_PLAFK DNA topoisomerase 2 (DNA topoisomerase II) Length = 1398 Score = 28.9 bits (63), Expect = 9.9 Identities = 14/41 (34%), Positives = 23/41 (56%) Frame = +2 Query: 5 EFCINRPRNYQDDKAFLERYLKKESTVSKPSIKDRIARLNN 127 EFC R + Y++ K++L L+KE + K +A +NN Sbjct: 1043 EFCYQRLKAYENRKSYLISKLEKEKRIISNKTKFILAIVNN 1083
Database: Non-redundant SwissProt sequences Posted date: Dec 6, 2005 7:40 AM Number of letters in database: 68,354,980 Number of sequences in database: 184,735 Database: swissprot.01 Posted date: Dec 6, 2005 8:18 AM Number of letters in database: 66,202,850 Number of sequences in database: 184,431 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 56,085,653 Number of Sequences: 369166 Number of extensions: 963578 Number of successful extensions: 1508 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 1485 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1508 length of database: 68,354,980 effective HSP length: 105 effective length of database: 48,957,805 effective search space used: 4406202450 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits)