Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_007_K16
(742 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9LX30|GCN2_ARATH Probable serine/threonine-protein kina... 41 0.004
sp|P43565|RIM15_YEAST Serine/threonine-protein kinase RIM15 41 0.004
sp|Q9UKI8|TLK1_HUMAN Serine/threonine-protein kinase tousle... 40 0.005
sp|Q8C0V0|TLK1_MOUSE Serine/threonine-protein kinase tousle... 40 0.005
sp|P42818|KPK1_ARATH Serine/threonine-protein kinase AtPK1/... 40 0.007
sp|Q39030|KPK19_ARATH Serine/threonine-protein kinase AtPK1... 39 0.011
sp|P41719|PK1_NPVHZ Serine/threonine-protein kinase 1 39 0.011
sp|P32023|KGP25_DROME cGMP-dependent protein kinase, isozym... 39 0.011
sp|Q03043|KGP24_DROME cGMP-dependent protein kinase, isozym... 39 0.011
sp|Q86UE8|TLK2_HUMAN Serine/threonine-protein kinase tousle... 39 0.015
>sp|Q9LX30|GCN2_ARATH Probable serine/threonine-protein kinase GCN2
Length = 1241
Score = 40.8 bits (94), Expect = 0.004
Identities = 23/80 (28%), Positives = 40/80 (50%), Gaps = 2/80 (2%)
Frame = +1
Query: 157 GNRYYAAPEYINNEKEMFDDKGDYWAVGVIFFHL--IFGCRPYQHIVSDKVKELSDFIIN 330
G +Y APE I + D+K D +++GV+FF L FG +H++ +K + +
Sbjct: 638 GTYFYTAPE-IEQDWPKIDEKADMYSLGVVFFELWHPFGTAMERHVILTNLKLKGELPLK 696
Query: 331 WKTKSFMQKSILKQRCKKYP 390
W + Q S+L++ P
Sbjct: 697 WVNEFPEQASLLRRLMSPSP 716
>sp|P43565|RIM15_YEAST Serine/threonine-protein kinase RIM15
Length = 1770
Score = 40.8 bits (94), Expect = 0.004
Identities = 24/65 (36%), Positives = 36/65 (55%), Gaps = 2/65 (3%)
Frame = +1
Query: 145 RRMWGNRYYAAPEYINNEKEMFDDKG-DYWAVGVIFFHLIFGCRPYQHIVSDKV-KELSD 318
++ +G Y APE I + E D+K D+W+VG IFF L+ G P+ D V K++
Sbjct: 1145 KKFFGTPDYLAPETIEGKGE--DNKQCDWWSVGCIFFELLLGYPPFHAETPDAVFKKILS 1202
Query: 319 FIINW 333
+I W
Sbjct: 1203 GVIQW 1207
>sp|Q9UKI8|TLK1_HUMAN Serine/threonine-protein kinase tousled-like 1 (Tousled-like kinase
1) (PKU-beta)
Length = 766
Score = 40.4 bits (93), Expect = 0.005
Identities = 22/79 (27%), Positives = 36/79 (45%), Gaps = 2/79 (2%)
Frame = +1
Query: 157 GNRYYAAPE--YINNEKEMFDDKGDYWAVGVIFFHLIFGCRPYQHIVSDKVKELSDFIIN 330
G +Y PE + E +K D W+VGVIFF ++G +P+ H S + + I+
Sbjct: 632 GTYWYLPPECFVVGKEPPKISNKVDVWSVGVIFFQCLYGRKPFGHNQSQQDILQENTILK 691
Query: 331 WKTKSFMQKSILKQRCKKY 387
F K ++ K +
Sbjct: 692 ATEVQFPVKPVVSSEAKAF 710
>sp|Q8C0V0|TLK1_MOUSE Serine/threonine-protein kinase tousled-like 1 (Tousled-like kinase
1)
Length = 766
Score = 40.4 bits (93), Expect = 0.005
Identities = 22/79 (27%), Positives = 36/79 (45%), Gaps = 2/79 (2%)
Frame = +1
Query: 157 GNRYYAAPE--YINNEKEMFDDKGDYWAVGVIFFHLIFGCRPYQHIVSDKVKELSDFIIN 330
G +Y PE + E +K D W+VGVIFF ++G +P+ H S + + I+
Sbjct: 632 GTYWYLPPECFVVGKEPPKISNKVDVWSVGVIFFQCLYGRKPFGHNQSQQDILQENTILK 691
Query: 331 WKTKSFMQKSILKQRCKKY 387
F K ++ K +
Sbjct: 692 ATEVQFPVKPVVSSEAKAF 710
>sp|P42818|KPK1_ARATH Serine/threonine-protein kinase AtPK1/AtPK6
Length = 465
Score = 40.0 bits (92), Expect = 0.007
Identities = 37/128 (28%), Positives = 53/128 (41%), Gaps = 8/128 (6%)
Frame = +1
Query: 34 DFGLAKRKCSFYELKEKCSYSSNCSSAHLLEPYLSTPRRMWGNRYYAAPEYINNEKEMFD 213
DFGLAK E +E +S C G Y APE + + D
Sbjct: 275 DFGLAK------EFEENTRSNSMC-----------------GTTEYMAPEIVRGKGH--D 309
Query: 214 DKGDYWAVGVIFFHLIFGCRPY--------QHIVSDKVKELSDFIINWKTKSFMQKSILK 369
D+W+VG++ + ++ G P+ Q IV DK+K L F+ N +ILK
Sbjct: 310 KAADWWSVGILLYEMLTGKPPFLGSKGKIQQKIVKDKIK-LPQFLSN------EAHAILK 362
Query: 370 QRCKKYPE 393
+K PE
Sbjct: 363 GLLQKEPE 370
>sp|Q39030|KPK19_ARATH Serine/threonine-protein kinase AtPK19 (Ribosomal-protein S6 kinase
homolog)
Length = 471
Score = 39.3 bits (90), Expect = 0.011
Identities = 36/128 (28%), Positives = 53/128 (41%), Gaps = 8/128 (6%)
Frame = +1
Query: 34 DFGLAKRKCSFYELKEKCSYSSNCSSAHLLEPYLSTPRRMWGNRYYAAPEYINNEKEMFD 213
DFGLAK E +E +S C G Y APE + + D
Sbjct: 281 DFGLAK------EFEENTRSNSMC-----------------GTTEYMAPEIVRGKGH--D 315
Query: 214 DKGDYWAVGVIFFHLIFGCRPY--------QHIVSDKVKELSDFIINWKTKSFMQKSILK 369
D+W+VG++ + ++ G P+ Q IV DK+K L F+ N ++LK
Sbjct: 316 KAADWWSVGILLYEMLTGKPPFLGSKGKIQQKIVKDKIK-LPQFLSN------EAHALLK 368
Query: 370 QRCKKYPE 393
+K PE
Sbjct: 369 GLLQKEPE 376
>sp|P41719|PK1_NPVHZ Serine/threonine-protein kinase 1
Length = 267
Score = 39.3 bits (90), Expect = 0.011
Identities = 19/84 (22%), Positives = 44/84 (52%)
Frame = +1
Query: 157 GNRYYAAPEYINNEKEMFDDKGDYWAVGVIFFHLIFGCRPYQHIVSDKVKELSDFIINWK 336
G + Y +PE + +++ +D D+WA+G++ + L+ G PY+H ++ D + +
Sbjct: 167 GTKEYMSPEKL--KRQNYDVHVDWWALGILTYELLIGHHPYKHSNDNEEDFDLDVLQQRQ 224
Query: 337 TKSFMQKSILKQRCKKYPELITVF 408
K + + L +K+ E + ++
Sbjct: 225 QKKLHKYNFLSSDAQKFLEAMLMY 248
>sp|P32023|KGP25_DROME cGMP-dependent protein kinase, isozyme 2 forms cD5/T2 (CGK)
(Foraging protein)
Length = 934
Score = 39.3 bits (90), Expect = 0.011
Identities = 21/52 (40%), Positives = 27/52 (51%), Gaps = 3/52 (5%)
Frame = +1
Query: 133 LSTPRRMW---GNRYYAAPEYINNEKEMFDDKGDYWAVGVIFFHLIFGCRPY 279
L T R+ W G Y APE I N D DYW++GV+ F L+ G P+
Sbjct: 772 LQTGRKTWTFCGTPEYVAPEVILNRGH--DISADYWSLGVLMFELLTGTPPF 821
>sp|Q03043|KGP24_DROME cGMP-dependent protein kinase, isozyme 2 forms cD4/T1/T3A/T3B (CGK)
(Foraging protein)
Length = 1088
Score = 39.3 bits (90), Expect = 0.011
Identities = 21/52 (40%), Positives = 27/52 (51%), Gaps = 3/52 (5%)
Frame = +1
Query: 133 LSTPRRMW---GNRYYAAPEYINNEKEMFDDKGDYWAVGVIFFHLIFGCRPY 279
L T R+ W G Y APE I N D DYW++GV+ F L+ G P+
Sbjct: 926 LQTGRKTWTFCGTPEYVAPEVILNRGH--DISADYWSLGVLMFELLTGTPPF 975
>sp|Q86UE8|TLK2_HUMAN Serine/threonine-protein kinase tousled-like 2 (Tousled-like kinase
2) (PKU-alpha)
Length = 772
Score = 38.9 bits (89), Expect = 0.015
Identities = 21/79 (26%), Positives = 36/79 (45%), Gaps = 2/79 (2%)
Frame = +1
Query: 157 GNRYYAAPE--YINNEKEMFDDKGDYWAVGVIFFHLIFGCRPYQHIVSDKVKELSDFIIN 330
G +Y PE + E +K D W+VGVIF+ ++G +P+ H S + + I+
Sbjct: 639 GTYWYLPPECFVVGKEPPKISNKVDVWSVGVIFYQCLYGRKPFGHNQSQQDILQENTILK 698
Query: 331 WKTKSFMQKSILKQRCKKY 387
F K ++ K +
Sbjct: 699 ATEVQFPPKPVVTPEAKAF 717
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 83,075,487
Number of Sequences: 369166
Number of extensions: 1685757
Number of successful extensions: 4617
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4451
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4604
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 6679696800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)