Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_007_K02
(763 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P06730|IF4E_HUMAN Eukaryotic translation initiation fact... 191 2e-48
sp|P29338|IF4E_RABIT Eukaryotic translation initiation fact... 189 5e-48
sp|P63073|IF4E_MOUSE Eukaryotic translation initiation fact... 189 5e-48
sp|Q9N0T5|IF4E_BOVIN Eukaryotic translation initiation fact... 189 7e-48
sp|P48597|IF4E_XENLA Eukaryotic translation initiation fact... 187 2e-47
sp|Q9DFS6|IF4EA_BRARE Eukaryotic translation initiation fac... 186 8e-47
sp|O77210|IF4E_APLCA Eukaryotic translation initiation fact... 181 2e-45
sp|Q9PW28|IF4EB_BRARE Eukaryotic translation initiation fac... 172 1e-42
sp|P48598|IF4E_DROME Eukaryotic translation initiation fact... 155 1e-37
sp|O61955|IF4E3_CAEEL Eukaryotic translation initiation fac... 154 2e-37
>sp|P06730|IF4E_HUMAN Eukaryotic translation initiation factor 4E (eIF4E) (eIF-4E) (mRNA
cap-binding protein) (eIF-4F 25 kDa subunit)
Length = 217
Score = 191 bits (484), Expect = 2e-48
Identities = 87/172 (50%), Positives = 117/172 (68%), Gaps = 2/172 (1%)
Frame = +2
Query: 95 PVKYVKESIYFRWAFYFFKYDRTKDWIDNMKKVIDFDTVEGFWGLMNFIAPPSNLPNGCD 274
P Y+K + RWA +FFK D++K W N++ + FDTVE FW L N I SNL GCD
Sbjct: 31 PEHYIKHPLQNRWALWFFKNDKSKTWQANLRLISKFDTVEDFWALYNHIQLSSNLMPGCD 90
Query: 275 YYIFKDGIQPMWEDERNKNGGMCLLTFDKKENFSYLDSCWLEILMLLVGETFEDDSDEIC 454
Y +FKDGI+PMWEDE+NK GG L+T +K++ S LD WLE L+ L+GE+F+D SD++C
Sbjct: 91 YSLFKDGIEPMWEDEKNKRGGRWLITLNKQQRRSDLDRFWLETLLCLIGESFDDYSDDVC 150
Query: 455 GAVFNKRGKGDRISVWTTDCSSAEAIKRIGEKVKEAVG--STCSVEYMAHAD 604
GAV N R KGD+I++WTT+C + EA+ IG KE +G + Y +HAD
Sbjct: 151 GAVVNVRAKGDKIAIWTTECENREAVTHIGRVYKERLGLPPKIVIGYQSHAD 202
>sp|P29338|IF4E_RABIT Eukaryotic translation initiation factor 4E (eIF4E) (eIF-4E) (mRNA
cap-binding protein) (eIF-4F 25 kDa subunit)
Length = 217
Score = 189 bits (481), Expect = 5e-48
Identities = 86/172 (50%), Positives = 117/172 (68%), Gaps = 2/172 (1%)
Frame = +2
Query: 95 PVKYVKESIYFRWAFYFFKYDRTKDWIDNMKKVIDFDTVEGFWGLMNFIAPPSNLPNGCD 274
P Y+K + RWA +FFK D++K W N++ + FDTVE FW L N I SNL GCD
Sbjct: 31 PEHYIKHPLQNRWALWFFKNDKSKTWQANLRLISKFDTVEDFWALYNHIQLSSNLMPGCD 90
Query: 275 YYIFKDGIQPMWEDERNKNGGMCLLTFDKKENFSYLDSCWLEILMLLVGETFEDDSDEIC 454
Y +FKDGI+PMWEDE+NK GG L+T +K++ S LD WLE L+ L+GE+F+D SD++C
Sbjct: 91 YSLFKDGIEPMWEDEKNKRGGRWLITLNKQQRRSDLDRFWLETLLCLIGESFDDYSDDVC 150
Query: 455 GAVFNKRGKGDRISVWTTDCSSAEAIKRIGEKVKEAVG--STCSVEYMAHAD 604
GAV N R KGD+I++WTT+C + +A+ IG KE +G + Y +HAD
Sbjct: 151 GAVVNVRAKGDKIAIWTTECENRDAVTHIGRVYKERLGLPPKIVIGYQSHAD 202
>sp|P63073|IF4E_MOUSE Eukaryotic translation initiation factor 4E (eIF4E) (eIF-4E) (mRNA
cap-binding protein) (eIF-4F 25 kDa subunit)
sp|P63074|IF4E_RAT Eukaryotic translation initiation factor 4E (eIF4E) (eIF-4E) (mRNA
cap-binding protein) (eIF-4F 25 kDa subunit)
Length = 217
Score = 189 bits (481), Expect = 5e-48
Identities = 86/172 (50%), Positives = 117/172 (68%), Gaps = 2/172 (1%)
Frame = +2
Query: 95 PVKYVKESIYFRWAFYFFKYDRTKDWIDNMKKVIDFDTVEGFWGLMNFIAPPSNLPNGCD 274
P Y+K + RWA +FFK D++K W N++ + FDTVE FW L N I SNL GCD
Sbjct: 31 PEHYIKHPLQNRWALWFFKNDKSKTWQANLRLISKFDTVEDFWALYNHIQLSSNLMPGCD 90
Query: 275 YYIFKDGIQPMWEDERNKNGGMCLLTFDKKENFSYLDSCWLEILMLLVGETFEDDSDEIC 454
Y +FKDGI+PMWEDE+NK GG L+T +K++ S LD WLE L+ L+GE+F+D SD++C
Sbjct: 91 YSLFKDGIEPMWEDEKNKRGGRWLITLNKQQRRSDLDRFWLETLLCLIGESFDDYSDDVC 150
Query: 455 GAVFNKRGKGDRISVWTTDCSSAEAIKRIGEKVKEAVG--STCSVEYMAHAD 604
GAV N R KGD+I++WTT+C + +A+ IG KE +G + Y +HAD
Sbjct: 151 GAVVNVRAKGDKIAIWTTECENRDAVTHIGRVYKERLGLPPKIVIGYQSHAD 202
>sp|Q9N0T5|IF4E_BOVIN Eukaryotic translation initiation factor 4E (eIF4E) (eIF-4E) (mRNA
cap-binding protein)
Length = 217
Score = 189 bits (480), Expect = 7e-48
Identities = 86/172 (50%), Positives = 116/172 (67%), Gaps = 2/172 (1%)
Frame = +2
Query: 95 PVKYVKESIYFRWAFYFFKYDRTKDWIDNMKKVIDFDTVEGFWGLMNFIAPPSNLPNGCD 274
P Y+K + RWA +FFK D++K W N++ + FDTVE FW L N I SNL GCD
Sbjct: 31 PEHYIKHPLQNRWALWFFKNDKSKTWQANLRLISKFDTVEDFWALYNHIQLSSNLMPGCD 90
Query: 275 YYIFKDGIQPMWEDERNKNGGMCLLTFDKKENFSYLDSCWLEILMLLVGETFEDDSDEIC 454
Y +FKDGI+PMWEDE+NK GG L+T +K++ LD WLE L+ L+GE+F+D SD++C
Sbjct: 91 YSLFKDGIEPMWEDEKNKRGGRWLITLNKQQRRGDLDRFWLETLLCLIGESFDDYSDDVC 150
Query: 455 GAVFNKRGKGDRISVWTTDCSSAEAIKRIGEKVKEAVG--STCSVEYMAHAD 604
GAV N R KGD+I++WTT+C + EA+ IG KE +G + Y +HAD
Sbjct: 151 GAVVNVRAKGDKIAIWTTECENREAVTHIGRVYKERLGLPPKIVIGYQSHAD 202
>sp|P48597|IF4E_XENLA Eukaryotic translation initiation factor 4E (eIF4E) (eIF-4E) (mRNA
cap-binding protein) (eIF-4F 25 kDa subunit)
Length = 213
Score = 187 bits (476), Expect = 2e-47
Identities = 85/174 (48%), Positives = 119/174 (68%), Gaps = 2/174 (1%)
Frame = +2
Query: 89 IFPVKYVKESIYFRWAFYFFKYDRTKDWIDNMKKVIDFDTVEGFWGLMNFIAPPSNLPNG 268
+ P +Y+K + RWA +FFK D++K W N++ + FDTVE FW L N I SNL +G
Sbjct: 25 VSPDQYIKHPLQNRWALWFFKNDKSKTWQANLRLISKFDTVEDFWALYNHIQLSSNLMSG 84
Query: 269 CDYYIFKDGIQPMWEDERNKNGGMCLLTFDKKENFSYLDSCWLEILMLLVGETFEDDSDE 448
CDY +FKDGI+PMWEDE+NK GG L+T +K++ + LD WLE LM L+GE+F++ SD+
Sbjct: 85 CDYSLFKDGIEPMWEDEKNKRGGRWLITLNKQQRRNDLDRFWLETLMCLIGESFDEHSDD 144
Query: 449 ICGAVFNKRGKGDRISVWTTDCSSAEAIKRIGEKVKEAVGSTCSV--EYMAHAD 604
+CGAV N R KGD+I++WTT+ + +A+ IG KE +G V Y +HAD
Sbjct: 145 VCGAVVNVRAKGDKIAIWTTEFENKDAVTHIGRVYKERLGLPAKVVIGYQSHAD 198
>sp|Q9DFS6|IF4EA_BRARE Eukaryotic translation initiation factor 4E-1A (eIF4E-1A) (mRNA
cap-binding protein)
Length = 215
Score = 186 bits (471), Expect = 8e-47
Identities = 87/169 (51%), Positives = 114/169 (67%), Gaps = 2/169 (1%)
Frame = +2
Query: 104 YVKESIYFRWAFYFFKYDRTKDWIDNMKKVIDFDTVEGFWGLMNFIAPPSNLPNGCDYYI 283
Y+K + RWA +FFK D++K W N++ + FDTVE FW L N I SNL +GCDY +
Sbjct: 32 YIKHPLQNRWALWFFKNDKSKTWQANLRLISKFDTVEDFWALYNHIQLSSNLMSGCDYSL 91
Query: 284 FKDGIQPMWEDERNKNGGMCLLTFDKKENFSYLDSCWLEILMLLVGETFEDDSDEICGAV 463
FKDGI+PMWEDERNK GG L+T K++ + LD WLE L+ LVGE F+D SD++CGAV
Sbjct: 92 FKDGIEPMWEDERNKRGGRWLITLSKQQRRADLDRFWLETLLCLVGEAFDDHSDDVCGAV 151
Query: 464 FNKRGKGDRISVWTTDCSSAEAIKRIGEKVKEAVG--STCSVEYMAHAD 604
N R KGD+I++WTTD + +AI IG KE +G + Y +HAD
Sbjct: 152 VNIRTKGDKIAIWTTDYENKDAIVHIGRVYKERLGVPPKVIIGYQSHAD 200
>sp|O77210|IF4E_APLCA Eukaryotic translation initiation factor 4E (eIF4E) (eIF-4E) (mRNA
cap-binding protein) (eIF-4F 25 kDa subunit)
Length = 215
Score = 181 bits (459), Expect = 2e-45
Identities = 84/188 (44%), Positives = 118/188 (62%), Gaps = 2/188 (1%)
Frame = +2
Query: 47 LTPDSDLHYLENK*IFPVKYVKESIYFRWAFYFFKYDRTKDWIDNMKKVIDFDTVEGFWG 226
+ PD + E + P +K + WA +FFK D+++DW DN++ + FDTVE FWG
Sbjct: 13 IRPDDEPEVEEERVPDPDMVIKHPLQNSWAMWFFKNDKSRDWKDNLRVITTFDTVEDFWG 72
Query: 227 LMNFIAPPSNLPNGCDYYIFKDGIQPMWEDERNKNGGMCLLTFDKKENFSYLDSCWLEIL 406
L N P S L +GCDY +FK GIQPMWED +NK GG L+ +K + ++LD WLE L
Sbjct: 73 LYNHTLPASKLQSGCDYSVFKAGIQPMWEDAQNKKGGRWLINLNKTQRQTHLDDFWLETL 132
Query: 407 MLLVGETFEDDSDEICGAVFNKRGKGDRISVWTTDCSSAEAIKRIGEKVKE--AVGSTCS 580
+ L+GE F++ S+EICGA N R KGD++ +WT D EA K+IG K+KE +V
Sbjct: 133 LCLIGEGFDEHSEEICGATVNIRNKGDKLGLWTRDAQKTEATKKIGIKLKESLSVPPKIV 192
Query: 581 VEYMAHAD 604
+ + AH+D
Sbjct: 193 IGFQAHSD 200
>sp|Q9PW28|IF4EB_BRARE Eukaryotic translation initiation factor 4E-1B (eIF4E-1B) (eIF4E)
Length = 214
Score = 172 bits (435), Expect = 1e-42
Identities = 80/168 (47%), Positives = 109/168 (64%), Gaps = 2/168 (1%)
Frame = +2
Query: 107 VKESIYFRWAFYFFKYDRTKDWIDNMKKVIDFDTVEGFWGLMNFIAPPSNLPNGCDYYIF 286
+K + RW +F+K D++K W DN++ + FDTVE FWGL N I PS L +GCDY +F
Sbjct: 32 MKHPLQNRWGLWFYKNDKSKMWQDNLRLITKFDTVEDFWGLYNNIQLPSKLSSGCDYSMF 91
Query: 287 KDGIQPMWEDERNKNGGMCLLTFDKKENFSYLDSCWLEILMLLVGETFEDDSDEICGAVF 466
KDGI+PMWED NK GG L+T K+ + LD WLE L+ L+GE F S +ICG+V
Sbjct: 92 KDGIEPMWEDRSNKCGGRWLITLAKQHRHTELDHFWLETLLCLIGEGFSSFSRDICGSVI 151
Query: 467 NKRGKGDRISVWTTDCSSAEAIKRIGEKVKEAVG--STCSVEYMAHAD 604
N R KGD+I++WT++ + E + IG K KE++G + Y AHAD
Sbjct: 152 NIRAKGDKIALWTSNAENCETVTYIGRKYKESLGLPQKLVIGYQAHAD 199
>sp|P48598|IF4E_DROME Eukaryotic translation initiation factor 4E (eIF4E) (eIF-4E) (mRNA
cap-binding protein) (eIF-4F 25 kDa subunit)
Length = 259
Score = 155 bits (391), Expect = 1e-37
Identities = 73/160 (45%), Positives = 103/160 (64%), Gaps = 2/160 (1%)
Frame = +2
Query: 131 WAFYFFKYDRTKDWIDNMKKVIDFDTVEGFWGLMNFIAPPSNLPNGCDYYIFKDGIQPMW 310
W ++ + DR+K W D ++ FDTVE FW L N I PPS + G DY +FK I+PMW
Sbjct: 87 WTLWYLENDRSKSWEDMQNEITSFDTVEDFWSLYNHIKPPSEIKLGSDYSLFKKNIRPMW 146
Query: 311 EDERNKNGGMCLLTFDKKENFSYLDSCWLEILMLLVGETFEDDSDEICGAVFNKRGKGDR 490
ED NK GG ++T +K + LD+ WL++L+ L+GE F D SD+ICGAV N RGK ++
Sbjct: 147 EDAANKQGGRWVITLNKSSK-TDLDNLWLDVLLCLIGEAF-DHSDQICGAVINIRGKSNK 204
Query: 491 ISVWTTDCSSAEAIKRIGEKVKEA--VGSTCSVEYMAHAD 604
IS+WT D ++ EA IG K+++A +G S++Y H D
Sbjct: 205 ISIWTADGNNEEAALEIGHKLRDALRLGRNNSLQYQLHKD 244
>sp|O61955|IF4E3_CAEEL Eukaryotic translation initiation factor 4E-3 (eIF4E-3) (eIF-4E-3)
(mRNA cap-binding protein) (eIF-4F 25 kDa subunit)
Length = 248
Score = 154 bits (390), Expect = 2e-37
Identities = 76/172 (44%), Positives = 102/172 (59%), Gaps = 2/172 (1%)
Frame = +2
Query: 95 PVKYVKESIYFRWAFYFFKYDRTKDWIDNMKKVIDFDTVEGFWGLMNFIAPPSNLPNGCD 274
P + + RWA ++ K DR K+W D +K V FDTVE FW L N I L G D
Sbjct: 26 PELLTRHPLQNRWALWYLKADRNKEWEDCLKMVSLFDTVEDFWSLYNHIQSAGGLNWGSD 85
Query: 275 YYIFKDGIQPMWEDERNKNGGMCLLTFDKKENFSYLDSCWLEILMLLVGETFEDDSDEIC 454
YY+FK+GI+PMWED N GG L+ DK+ LD WLE+LM +VGE F++ D IC
Sbjct: 86 YYLFKEGIKPMWEDVNNVQGGRWLVVVDKQRRTQLLDHYWLELLMAIVGEQFDEYGDYIC 145
Query: 455 GAVFNKRGKGDRISVWTTDCSSAEAIKRIGEKVKE--AVGSTCSVEYMAHAD 604
GAV N R KGD++S+WT D + + RIG+ +K+ ++ T + Y H D
Sbjct: 146 GAVVNVRQKGDKVSLWTRDATRDDVNLRIGQVLKQKLSIPDTEILRYEVHKD 197
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 83,904,410
Number of Sequences: 369166
Number of extensions: 1658337
Number of successful extensions: 4350
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4153
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4313
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 7018522000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)