Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_007_J04
(536 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O24454|UBP3_ARATH Ubiquitin carboxyl-terminal hydrolase ... 66 5e-11
sp|Q8LAM0|UBP4_ARATH Ubiquitin carboxyl-terminal hydrolase ... 63 4e-10
sp|O94269|UBP3_SCHPO Probable ubiquitin carboxyl-terminal h... 61 2e-09
sp|Q14694|UBP10_HUMAN Ubiquitin carboxyl-terminal hydrolase... 53 4e-07
sp|P52479|UBP10_MOUSE Ubiquitin carboxyl-terminal hydrolase... 53 4e-07
sp|P51784|UBP11_HUMAN Ubiquitin carboxyl-terminal hydrolase... 51 2e-06
sp|Q9Y6I4|UBP3_HUMAN Ubiquitin carboxyl-terminal hydrolase ... 50 3e-06
sp|Q91W36|UBP3_MOUSE Ubiquitin carboxyl-terminal hydrolase ... 48 2e-05
sp|Q8R5H1|UBP15_MOUSE Ubiquitin carboxyl-terminal hydrolase... 48 2e-05
sp|Q9Y4E8|UBP15_HUMAN Ubiquitin carboxyl-terminal hydrolase... 48 2e-05
>sp|O24454|UBP3_ARATH Ubiquitin carboxyl-terminal hydrolase 3 (Ubiquitin thiolesterase 3)
(Ubiquitin-specific processing protease 3)
(Deubiquitinating enzyme 3) (AtUBP3)
Length = 371
Score = 66.2 bits (160), Expect = 5e-11
Identities = 38/113 (33%), Positives = 56/113 (49%)
Frame = +2
Query: 23 PSLLILQLKRFTYTVDVGXXXXXXXXXXXXLLTIPEDSISSTIKQSGDVAYEIFAVVYHI 202
P +L++ LKRF Y +G L E +S+T++ DV Y +FAVV H+
Sbjct: 252 PHILVIHLKRFKYIEQLGRYKKLSYRVVFPL----ELKLSNTVEPYADVEYSLFAVVVHV 307
Query: 203 GEGVTKGHYTAAVRDNGCWYYLDDSNVFLIQGKQSADWLLNSSSADIPSQLLH 361
G G GHY + V+ + W + DD NV +I+ +SA SS + S H
Sbjct: 308 GSGPNHGHYVSLVKSHNHWLFFDDENVEMIE--ESAVQTFFGSSQEYSSNTDH 358
>sp|Q8LAM0|UBP4_ARATH Ubiquitin carboxyl-terminal hydrolase 4 (Ubiquitin thiolesterase 4)
(Ubiquitin-specific processing protease 4)
(Deubiquitinating enzyme 4) (AtUBP4)
Length = 365
Score = 63.2 bits (152), Expect = 4e-10
Identities = 36/113 (31%), Positives = 56/113 (49%)
Frame = +2
Query: 23 PSLLILQLKRFTYTVDVGXXXXXXXXXXXXLLTIPEDSISSTIKQSGDVAYEIFAVVYHI 202
P +L++ LKRF Y +G L E +S+T+ + D+ Y +FAVV H+
Sbjct: 247 PHILVIHLKRFKYMEQLGRYKKLSYRVVFPL----ELKLSNTVDEYVDIEYSLFAVVVHV 302
Query: 203 GEGVTKGHYTAAVRDNGCWYYLDDSNVFLIQGKQSADWLLNSSSADIPSQLLH 361
G G GHY + V+ + W + DD +V +I+ +SA SS + S H
Sbjct: 303 GSGPNHGHYVSLVKSHNHWLFFDDESVEIIE--ESAVQTFFGSSQEYSSNTDH 353
>sp|O94269|UBP3_SCHPO Probable ubiquitin carboxyl-terminal hydrolase 3 (Ubiquitin
thiolesterase 3) (Ubiquitin-specific processing protease
3) (Deubiquitinating enzyme 3)
Length = 512
Score = 60.8 bits (146), Expect = 2e-09
Identities = 41/143 (28%), Positives = 64/143 (44%), Gaps = 2/143 (1%)
Frame = +2
Query: 20 LPSLLILQLKRFTYTVDVGXXXXXXXXXXXXLLTIPEDSISSTIKQSGDVAYEIFAVVYH 199
LP +LIL LKRF Y G L+IP++ S +++ S Y++ AVVYH
Sbjct: 385 LPPVLILHLKRFFYEASGGTQKNYKPIAYPARLSIPQNVFSPSVRGSIHPEYDLNAVVYH 444
Query: 200 IGEGVTKGHYTAAVR--DNGCWYYLDDSNVFLIQGKQSADWLLNSSSADIPSQLLHSLDL 373
G + GHYT V+ D W+ +DD+++ +P + + +L
Sbjct: 445 HGTSASGGHYTVDVQQLDKSGWFRIDDTHIH-----------------RVPIHDVENSEL 487
Query: 374 KACAPRTACVSGRRSPYLIFYRK 442
A + G R YL+FY +
Sbjct: 488 SADPSLSKLGHGDRVAYLLFYTR 510
>sp|Q14694|UBP10_HUMAN Ubiquitin carboxyl-terminal hydrolase 10 (Ubiquitin thiolesterase 10)
(Ubiquitin-specific processing protease 10)
(Deubiquitinating enzyme 10)
Length = 798
Score = 53.1 bits (126), Expect = 4e-07
Identities = 44/148 (29%), Positives = 59/148 (39%), Gaps = 5/148 (3%)
Frame = +2
Query: 17 RLPSLLILQLKRFTYTVDVGXXXXXXXXXXXXLLTIPEDSISSTIKQSG---DVAYEIFA 187
+LP +L+L LKRF Y G L I ++ +S +K Y +FA
Sbjct: 677 KLPPVLVLHLKRFVYEKTGGCQKLIKNIEYPVDLEISKELLSPGVKNKNFKCHRTYRLFA 736
Query: 188 VVYHIGEGVTKGHYTAAVRDNGC--WYYLDDSNVFLIQGKQSADWLLNSSSADIPSQLLH 361
VVYH G T GHYT V G W +DD V +I Q
Sbjct: 737 VVYHHGNSATGGHYTTDVFQIGLNGWLRIDDQTVKVINQYQV------------------ 778
Query: 362 SLDLKACAPRTACVSGRRSPYLIFYRKI 445
+K A RTA YL++YR++
Sbjct: 779 ---VKPTAERTA--------YLLYYRRV 795
>sp|P52479|UBP10_MOUSE Ubiquitin carboxyl-terminal hydrolase 10 (Ubiquitin thiolesterase
10) (Ubiquitin-specific processing protease 10)
(Deubiquitinating enzyme 10)
Length = 792
Score = 53.1 bits (126), Expect = 4e-07
Identities = 36/101 (35%), Positives = 44/101 (43%), Gaps = 5/101 (4%)
Frame = +2
Query: 17 RLPSLLILQLKRFTYTVDVGXXXXXXXXXXXXLLTIPEDSISSTIKQSG---DVAYEIFA 187
+LP +L+L LKRF Y G L I + +S IK Y +FA
Sbjct: 671 KLPPVLVLHLKRFVYEKTGGCQKLVKNIDYPVDLEISRELLSPGIKNKNFKCQRTYRLFA 730
Query: 188 VVYHIGEGVTKGHYTAAVRDNGC--WYYLDDSNVFLIQGKQ 304
VVYH G T GHYT V G W +DD V +I Q
Sbjct: 731 VVYHHGNSATGGHYTTDVFQIGLNGWLRIDDQTVKVINQYQ 771
>sp|P51784|UBP11_HUMAN Ubiquitin carboxyl-terminal hydrolase 11 (Ubiquitin thiolesterase 11)
(Ubiquitin-specific processing protease 11)
(Deubiquitinating enzyme 11)
Length = 920
Score = 50.8 bits (120), Expect = 2e-06
Identities = 41/142 (28%), Positives = 63/142 (44%), Gaps = 6/142 (4%)
Frame = +2
Query: 20 LPSLLILQLKRFTYTVDVGXXXXXXXXXXXXLLTIPEDSISSTIKQSGDV-AYEIFAVVY 196
LP +LI+ LKRF+YT L E I + + ++ Y++ AV
Sbjct: 777 LPEILIIHLKRFSYTKFSREKLDTLVEFPIRDLDFSEFVIQPQNESNPELYKYDLIAVSN 836
Query: 197 HIGEGVTKGHYT--AAVRDNGCWYYLDDSNVFLIQGKQ---SADWLLNSSSADIPSQLLH 361
H G G+ GHYT A +D+G W+Y DD++V + Q A ++L D+ +LL
Sbjct: 837 HYG-GMRDGHYTTFACNKDSGQWHYFDDNSVSPVNENQIESKAAYVLFYQRQDVARRLLS 895
Query: 362 SLDLKACAPRTACVSGRRSPYL 427
AC S S ++
Sbjct: 896 PAGSSGAPASPACSSPPSSEFM 917
>sp|Q9Y6I4|UBP3_HUMAN Ubiquitin carboxyl-terminal hydrolase 3 (Ubiquitin thiolesterase 3)
(Ubiquitin-specific processing protease 3)
(Deubiquitinating enzyme 3)
Length = 521
Score = 50.1 bits (118), Expect = 3e-06
Identities = 34/97 (35%), Positives = 44/97 (45%), Gaps = 6/97 (6%)
Frame = +2
Query: 17 RLPSLLILQLKRFTYT------VDVGXXXXXXXXXXXXLLTIPEDSISSTIKQSGDVAYE 178
+LP +L L LKRF +T VD L PE+S + Y+
Sbjct: 403 KLPKVLCLHLKRFHWTAYLRNKVDTYVEFPLRGLDMKWYLLEPENSGPESC------LYD 456
Query: 179 IFAVVYHIGEGVTKGHYTAAVRDNGCWYYLDDSNVFL 289
+ AVV H G GV GHYTA G W++ +DS V L
Sbjct: 457 LAAVVVHHGSGVGSGHYTAYATHEGRWFHFNDSTVTL 493
>sp|Q91W36|UBP3_MOUSE Ubiquitin carboxyl-terminal hydrolase 3 (Ubiquitin thiolesterase 3)
(Ubiquitin-specific processing protease 3)
(Deubiquitinating enzyme 3)
Length = 520
Score = 47.8 bits (112), Expect = 2e-05
Identities = 33/95 (34%), Positives = 42/95 (44%), Gaps = 6/95 (6%)
Frame = +2
Query: 17 RLPSLLILQLKRFTYT------VDVGXXXXXXXXXXXXLLTIPEDSISSTIKQSGDVAYE 178
+LP L L LKRF +T VD L PE+S + Y+
Sbjct: 402 KLPKALCLHLKRFHWTAYLRNKVDTYVQFPLRGLDMKCYLLEPENSGPDSC------LYD 455
Query: 179 IFAVVYHIGEGVTKGHYTAAVRDNGCWYYLDDSNV 283
+ AVV H G GV GHYTA G W++ +DS V
Sbjct: 456 LAAVVVHHGSGVGSGHYTAYAVHEGRWFHFNDSTV 490
>sp|Q8R5H1|UBP15_MOUSE Ubiquitin carboxyl-terminal hydrolase 15 (Ubiquitin thiolesterase 15)
(Ubiquitin-specific processing protease 15)
(Deubiquitinating enzyme 15)
Length = 981
Score = 47.8 bits (112), Expect = 2e-05
Identities = 32/90 (35%), Positives = 46/90 (51%), Gaps = 2/90 (2%)
Frame = +2
Query: 20 LPSLLILQLKRFTYTVDVGXXXXXXXXXXXXLLTIPEDSISSTIKQSGDVAYEIFAVVYH 199
LP +L++ LKRF+Y+ + L + E I+ +G Y + AV H
Sbjct: 827 LPPVLVVHLKRFSYSRYMRDKLDTLVDFPISDLDMSEFLINPN---AGPCRYNLIAVSNH 883
Query: 200 IGEGVTKGHYTAAVR--DNGCWYYLDDSNV 283
G G+ GHYTA + D+G WYY DDS+V
Sbjct: 884 YG-GMGGGHYTAFAKNKDDGKWYYFDDSSV 912
>sp|Q9Y4E8|UBP15_HUMAN Ubiquitin carboxyl-terminal hydrolase 15 (Ubiquitin thiolesterase 15)
(Ubiquitin-specific processing protease 15)
(Deubiquitinating enzyme 15) (Unph-2) (Unph4)
Length = 981
Score = 47.8 bits (112), Expect = 2e-05
Identities = 32/90 (35%), Positives = 46/90 (51%), Gaps = 2/90 (2%)
Frame = +2
Query: 20 LPSLLILQLKRFTYTVDVGXXXXXXXXXXXXLLTIPEDSISSTIKQSGDVAYEIFAVVYH 199
LP +L++ LKRF+Y+ + L + E I+ +G Y + AV H
Sbjct: 827 LPPVLVVHLKRFSYSRYMRDKLDTLVDFPINDLDMSEFLINPN---AGPCRYNLIAVSNH 883
Query: 200 IGEGVTKGHYTAAVR--DNGCWYYLDDSNV 283
G G+ GHYTA + D+G WYY DDS+V
Sbjct: 884 YG-GMGGGHYTAFAKNKDDGKWYYFDDSSV 912
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 57,466,511
Number of Sequences: 369166
Number of extensions: 1097896
Number of successful extensions: 2787
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2705
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2763
length of database: 68,354,980
effective HSP length: 103
effective length of database: 49,327,275
effective search space used: 3699545625
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)