Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_007_I17
(846 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O95433|AHSA1_HUMAN Activator of 90 kDa heat shock protei... 173 6e-43
sp|Q8BK64|AHSA1_MOUSE Activator of 90 kDa heat shock protei... 172 1e-42
sp|Q12449|AHA1_YEAST Hsp90 co-chaperone AHA1 (Activator of ... 88 3e-17
sp|Q9P782|YNY8_SCHPO Hypothetical protein C1711.08 in chrom... 76 1e-13
sp|P53834|HCH1_YEAST Hsp90 co-chaperone HCH1 (High-copy Hsp... 63 9e-10
sp|Q02224|CENPE_HUMAN Centromeric protein E (CENP-E protein) 33 0.76
sp|Q98QL0|RNR_MYCPU Ribonuclease R (RNase R) (VacB protein ... 33 0.76
sp|O42184|REST_CHICK Restin (Cytoplasmic linker protein 170... 33 0.76
sp|Q97T80|ZMPC_STRPN Zinc metalloprotease zmpC precursor (M... 33 1.3
sp|P36251|RPOB_LIBAS DNA-directed RNA polymerase beta chain... 33 1.3
>sp|O95433|AHSA1_HUMAN Activator of 90 kDa heat shock protein ATPase homolog 1 (AHA1)
(p38)
Length = 338
Score = 173 bits (438), Expect = 6e-43
Identities = 91/230 (39%), Positives = 137/230 (59%), Gaps = 5/230 (2%)
Frame = +3
Query: 48 EGDPRWIVEEREDAKNVNNWHWSEKNATQWSKDMFKKLLVGLVIDNNEYFFEITDLSKIE 227
EGDPRWIVEER DA NVNNWHW+E++A+ WS D K L + + + N E E+T++SK++
Sbjct: 6 EGDPRWIVEERADATNVNNWHWTERDASNWSTDKLKTLFLAVQVQNEEGKCEVTEVSKLD 65
Query: 228 GEAIANNRKAKVIVFYEWVIEAEWKGHLKNNINKTEFKGKFDIPNLSEEFDSSEIDVNVS 407
GEA NNRK K+I FYEW ++ W G K+ + ++KG +IPNLS+E E++++VS
Sbjct: 66 GEASINNRKGKLIFFYEWSVKLNWTGTSKSGV---QYKGHVEIPNLSDENSVDEVEISVS 122
Query: 408 VESHSNDANQMKDYIRLECSKVIREKLGKYISDLKTEYSKDLILPTKKVSNDGPVVVSNA 587
+ D N + ++ E K++RE +G YIS LKTE+++ +ILPT + P V A
Sbjct: 123 LAKDEPDTN-LVALMKEEGVKLLREAMGIYISTLKTEFTQGMILPTMNGESVDP-VGQPA 180
Query: 588 YKQTMNEIVVSKSHKNS-----SLTFLSLELNEEFFCTPSDLYQVFINSD 722
K + + S + + + L E F +P +LY+VF +
Sbjct: 181 LKTEERKAKPAPSKTQARPVGVKIPTCKITLKETFLTSPEELYRVFTTQE 230
>sp|Q8BK64|AHSA1_MOUSE Activator of 90 kDa heat shock protein ATPase homolog 1 (AHA1)
Length = 338
Score = 172 bits (436), Expect = 1e-42
Identities = 90/229 (39%), Positives = 138/229 (60%), Gaps = 4/229 (1%)
Frame = +3
Query: 48 EGDPRWIVEEREDAKNVNNWHWSEKNATQWSKDMFKKLLVGLVIDNNEYFFEITDLSKIE 227
EGDPRWIVEER DA NVNNWHW+E++A+ WS + K L + + ++N E E+T+++K++
Sbjct: 6 EGDPRWIVEERADATNVNNWHWTERDASNWSTEKLKTLFLAVRVENEEGKCEVTEVNKLD 65
Query: 228 GEAIANNRKAKVIVFYEWVIEAEWKGHLKNNINKTEFKGKFDIPNLSEEFDSSEIDVNVS 407
GEA NNRK K+I FYEW I+ W G K+ + ++KG +IPNLS+E E++++VS
Sbjct: 66 GEASINNRKGKLIFFYEWTIKLNWTGTSKSGV---QYKGHVEIPNLSDENSVDEVEISVS 122
Query: 408 VESHSNDANQMKDYIRLECSKVIREKLGKYISDLKTEYSKDLILPTKKVSNDGPVVVSNA 587
+ D N + ++ + K++RE +G YIS LKTE+++ +ILPT + PV
Sbjct: 123 LAKDEPDTN-LVALMKEDGVKLLREAVGIYISTLKTEFTQGMILPTVNGESVDPVGQPAL 181
Query: 588 YKQTMN-EIVVSKSHKNS---SLTFLSLELNEEFFCTPSDLYQVFINSD 722
+T + SKS + + L E F +P +LY+VF +
Sbjct: 182 KTETCKAKSAPSKSQAKPVGVKIPTCKITLKETFLTSPEELYRVFTTQE 230
>sp|Q12449|AHA1_YEAST Hsp90 co-chaperone AHA1 (Activator of Hsp90 ATPase protein 1)
Length = 350
Score = 87.8 bits (216), Expect = 3e-17
Identities = 58/231 (25%), Positives = 110/231 (47%), Gaps = 23/231 (9%)
Frame = +3
Query: 93 NVNNWHWSEKNATQWSKDMFKKLLVGLVIDN--NEYFFEITDLSKIEGEAIANNRKAKVI 266
N NNWHW +KN W+K+ FK+ LVG+ + ++ + +I +S IEG+ N RK KVI
Sbjct: 5 NPNNWHWVDKNCIGWAKEYFKQKLVGVEAGSVKDKKYAKIKSVSSIEGDCEVNQRKGKVI 64
Query: 267 VFYEWVIEAEWKGHLKNNINKT-EFKGKFDIPNLSEEFDSSEIDVNVSVESHSNDANQMK 443
++ I +GH+ + F+G ++P ++ + ++S ++S+ +++ ++ K
Sbjct: 65 SLFDLKITVLIEGHVDSKDGSALPFEGSINVPEVAFDSEASSYQFDISIFKETSELSEAK 124
Query: 444 DYIRLECSKVIREKLGKYISDLKTEYSKDLILPTKKVSND---------GPVVVSNAYKQ 596
IR E +R+ ++ DL + D+ +P +V ++ + +A K
Sbjct: 125 PLIRSELLPKLRQIFQQFGKDLLATHGNDIQVPESQVKSNYTRGNQKSSFTEIKDSASKP 184
Query: 597 TMNEIVVS-----------KSHKNSSLTFLSLELNEEFFCTPSDLYQVFIN 716
N + S K +N S S+ L F S+LY+ F++
Sbjct: 185 KKNALPSSTSTSAPVSSTNKVPQNGSGNSTSIYLEPTFNVPSSELYETFLD 235
>sp|Q9P782|YNY8_SCHPO Hypothetical protein C1711.08 in chromosome II
Length = 336
Score = 76.3 bits (186), Expect = 1e-13
Identities = 45/170 (26%), Positives = 81/170 (47%), Gaps = 2/170 (1%)
Frame = +3
Query: 93 NVNNWHWSEKNATQWSKDMFKKLLVGLVIDNNEYFFEITDLSKIEGEAIANNRKAKVIVF 272
N NNWHW+ K+ WS + F K L + IT ++ EG+ + RK KVI
Sbjct: 7 NPNNWHWTSKDCRVWSHEYFNKELPKIQASEGPTSARITQVNSCEGDVDVSMRKRKVITI 66
Query: 273 YEWVIEAEWKGHLKNNINKTEFKGKFDIPNLSEEFDSSEIDVNVSVESHSNDANQMKDYI 452
++ I+ E+KG K+ + T G P LS + S+ ++ + S S + +K+ +
Sbjct: 67 FDLKIQMEFKGETKDGVEAT---GSITCPELSYDLGYSDYVFDIDIYSASKEKEPIKELV 123
Query: 453 RLECSKVIREKLGKYISDLKTEYSKDLILPTKKVSNDGP--VVVSNAYKQ 596
R + IR+ + L + D+ L T++ + + + V +++KQ
Sbjct: 124 REKIIPQIRQLFSGFSQVLLQTHGDDVYLSTEEHNGNAARGLPVHSSFKQ 173
>sp|P53834|HCH1_YEAST Hsp90 co-chaperone HCH1 (High-copy Hsp90 suppressor protein 1)
Length = 153
Score = 63.2 bits (152), Expect = 9e-10
Identities = 48/155 (30%), Positives = 71/155 (45%), Gaps = 8/155 (5%)
Frame = +3
Query: 93 NVNNWHWSEKNATQWSKDMFKKLLVGL--VIDNNEYFFEITDLSKIEGEAIANNRKAKVI 266
N NNWHW +KN WSKD L L V + + E+T +S I G++ + RK K I
Sbjct: 5 NPNNWHWVDKNTLPWSKDYLNGKLTSLSTVSSDGKSKIELTQVSSITGDSNVSQRKGKPI 64
Query: 267 VFYEWVIEAEWK-GHLKNNINKTE-----FKGKFDIPNLSEEFDSSEIDVNVSVESHSND 428
+++ + K +L N + + GK +IP EF E D+ + S
Sbjct: 65 CYFDLQLSMNVKVTNLDTNKDDEDDDGILADGKLEIP----EFMHDESDIPIL----SQG 116
Query: 429 ANQMKDYIRLECSKVIREKLGKYISDLKTEYSKDL 533
+ +R E + E L KY DL E+SKD+
Sbjct: 117 FDAFDGLVRSEFVPKVVETLLKYQDDLIKEHSKDI 151
>sp|Q02224|CENPE_HUMAN Centromeric protein E (CENP-E protein)
Length = 2663
Score = 33.5 bits (75), Expect = 0.76
Identities = 42/161 (26%), Positives = 63/161 (39%), Gaps = 12/161 (7%)
Frame = +3
Query: 219 KIEGEAIANNRKAKVIVFYEWVIEAEWKGHLKNNINKTEFKGKFDIPNLSEEFDSSEIDV 398
K E + + +NRK EW+ LKN I K + I ++ F++ I +
Sbjct: 2217 KTEFQQVLSNRKEMTQFLEEWLNTRFDIEKLKNGIQKENDR----ICQVNNFFNNRIIAI 2272
Query: 399 -NVSVESHSNDANQMKDYIR-LECSKVIREKLGKYISDLKTEYSKDLILPTKKVSNDGPV 572
N S E A K++ + L+ K EKL K LKT + + N P
Sbjct: 2273 MNESTEFEERSATISKEWEQDLKSLKEKNEKLFKNYQTLKTSLASGAQVNPTTQDNKNPH 2332
Query: 573 VVSNAYKQT----------MNEIVVSKSHKNSSLTFLSLEL 665
V S A + T ++E S HK S + + EL
Sbjct: 2333 VTSRATQLTTEKIRELENSLHEAKESAMHKESKIIKMQKEL 2373
>sp|Q98QL0|RNR_MYCPU Ribonuclease R (RNase R) (VacB protein homolog)
Length = 725
Score = 33.5 bits (75), Expect = 0.76
Identities = 50/213 (23%), Positives = 84/213 (39%), Gaps = 44/213 (20%)
Frame = +3
Query: 129 TQWSKDMFKKLLVGLVIDNNE-YFFEITDLSKIEGEAIANNRKAKVI------------- 266
+Q SK +F L GL++ NN+ F ++ ++ KI+G +NR I
Sbjct: 32 SQLSKALFSLLDQGLIVKNNDGNFIKVNEIKKIQGIFKQSNRNFAFIETADEQSYFVAKA 91
Query: 267 -------------VFYEWVIEAEWKGHLKNNINKT---EFKGKFDIPNLSEEFDSSE--- 389
+ YE E + K + I K E G I N + F++ E
Sbjct: 92 HFNGAINSFEVEAIVYESPFEKDKKYAVVKKILKNTHPEIIGFIKISNGIKYFNAFEESF 151
Query: 390 ------IDVNVSVESHSNDANQMKDYIRLECSKVIREKL----GKYISDLKTE-YSKDLI 536
+D N+ VE D I + K++ +K+ K +S LK+ Y D++
Sbjct: 152 RSYKFFVDQNIDVEE--------DDVILVVVEKIVDQKIYVNFVKKVSTLKSNFYQIDIV 203
Query: 537 LPTKKVSNDGPVVVSNAYKQTMNEIVVSKSHKN 635
L K D P V + + + V+ K ++N
Sbjct: 204 LEKSKTIIDFPEDVLDE-SALIEDHVIEKDYQN 235
>sp|O42184|REST_CHICK Restin (Cytoplasmic linker protein 170) (CLIP-170)
Length = 1433
Score = 33.5 bits (75), Expect = 0.76
Identities = 29/120 (24%), Positives = 56/120 (46%), Gaps = 3/120 (2%)
Frame = +3
Query: 321 INKTEFKGKFDIPNLSEEFDSSEIDV-NVSVESHSNDANQMKDYIRLECSKVIREKLGKY 497
+ K +GK +I LSE+ ++E + N+ E SN +++ E + EK
Sbjct: 769 LQKANSEGKLEIQKLSEQLQAAEKQIQNLETEKVSNLTKELQG---KEQKLLDLEKNLSA 825
Query: 498 ISDLKTEYSKDLILPTKKVSN--DGPVVVSNAYKQTMNEIVVSKSHKNSSLTFLSLELNE 671
++ +K K+L L +K ++ DG A ++T+N++ + K +S EL +
Sbjct: 826 VNQVKDSLEKELQLLKEKFTSAVDGAENAQRAMQETINKL----NQKEEQFALMSSELEQ 881
>sp|Q97T80|ZMPC_STRPN Zinc metalloprotease zmpC precursor (MMP-9 protease)
Length = 1856
Score = 32.7 bits (73), Expect = 1.3
Identities = 31/127 (24%), Positives = 55/127 (43%), Gaps = 3/127 (2%)
Frame = +3
Query: 237 IANNRKAKVIVFYEWVIEAEWKGHLKNNINKT-EFKGKFDIPNLSEEFDSSEIDVNVSVE 413
I N K +VI ++ K HL N+ + E + + E+ EI SVE
Sbjct: 548 IENPAKEQVISGLDYYTPYTVKTHLTYNLGENNEENTETSTQDFQLEYKKIEIKDIDSVE 607
Query: 414 SHSNDANQMKDYIRLECSKVIREKLGKYISDLKTEYSKDLILPTKKV--SNDGPVVVSNA 587
+ + ++ + Y+ L + KY +K++ K++ LP K + + DG V+ A
Sbjct: 608 LYGKENDRYRRYLSLSEAPT---DTAKYFVKVKSDRFKEMYLPVKSITENTDGTYKVTVA 664
Query: 588 YKQTMNE 608
Q + E
Sbjct: 665 VDQLVEE 671
>sp|P36251|RPOB_LIBAS DNA-directed RNA polymerase beta chain (RNAP beta subunit)
(Transcriptase beta chain) (RNA polymerase beta subunit)
Length = 178
Score = 32.7 bits (73), Expect = 1.3
Identities = 33/109 (30%), Positives = 48/109 (44%), Gaps = 2/109 (1%)
Frame = +3
Query: 330 TEFKGKFDIPNLSEEFDSSEIDVNVSVESHSNDANQMKDYIRLECSKVIREKLGKYISDL 509
T F G + +S EFD + DV+ + A +K +RL V K I D+
Sbjct: 66 TAFSGAAMLEFVSYEFDPPKFDVDDCLWRDLTYAVPLKITLRLIVFDVDEFTGAKSIKDI 125
Query: 510 KTE--YSKDLILPTKKVSNDGPVVVSNAYKQTMNEIVVSKSHKNSSLTF 650
K + Y DL L TK DG V+ + IVVS+ H++ + F
Sbjct: 126 KEQSIYMGDLPLMTK----DGTFVIKGTQR-----IVVSQLHRSPGIHF 165
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 82,598,927
Number of Sequences: 369166
Number of extensions: 1516072
Number of successful extensions: 4279
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4146
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4271
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8293644780
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)