Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_007_E10
(802 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q8BXR6|PELI3_MOUSE Pellino protein homolog 3 (Pellino 3) 94 3e-19
sp|Q8N2H9|PELI3_HUMAN Pellino protein homolog 3 (Pellino 3) 93 7e-19
sp|Q8C669|PELI1_MOUSE Pellino protein homolog 1 (Pellino 1) 93 1e-18
sp|Q96FA3|PELI1_HUMAN Pellino protein homolog 1 (Pellino 1)... 93 1e-18
sp|Q9HAT8|PELI2_HUMAN Pellino protein homolog 2 (Pellino 2) 89 2e-17
sp|Q8BST6|PELI2_MOUSE Pellino protein homolog 2 (Pellino 2) 89 2e-17
sp|O77237|PELI_DROME Pellino protein 85 2e-16
sp|Q9XT84|IL2_DELLE Interleukin-2 precursor (IL-2) (T-cell ... 36 0.14
sp|P46873|OSM3_CAEEL Osmotic avoidance abnormal protein 3 (... 35 0.31
sp|Q9PQF2|DNAK_UREPA Chaperone protein dnaK (Heat shock pro... 33 0.91
>sp|Q8BXR6|PELI3_MOUSE Pellino protein homolog 3 (Pellino 3)
Length = 445
Score = 94.4 bits (233), Expect = 3e-19
Identities = 49/103 (47%), Positives = 59/103 (57%), Gaps = 3/103 (2%)
Frame = +2
Query: 464 TPGPE---TVSRFACRIFINRKPPHTARIYAAGFDGNKNIFLGENAIKWEADKKIYDGFT 634
T GP T+SR+ACRI +R+PP+TARIYAAGFD + NIFLGE A KW + DG T
Sbjct: 151 TEGPSAQSTISRYACRILCDRRPPYTARIYAAGFDASSNIFLGERAAKWRTPDGLMDGLT 210
Query: 635 TNGVMICHPHVNFDPDIRSPDALLKTKECQSSFANSSWREVSV 763
TNGV++ HP F D A WRE+SV
Sbjct: 211 TNGVLVMHPAGGFSED----------------SAPGVWREISV 237
Score = 75.1 bits (183), Expect = 2e-13
Identities = 39/102 (38%), Positives = 65/102 (63%)
Frame = +3
Query: 120 IVYGYLIVLGTNGRKSWRYSDNKKETNKLKVVQRSQSNGIKQIKIGRYKEAKSSQAVQSK 299
I YG LIVLG NG + D + ++L + +R +NG+K + S+A+ ++
Sbjct: 42 IKYGELIVLGYNGCLA--SGDKGRRRSRLALSRRPHANGVKPDVMHHISTPLVSKALSNR 99
Query: 300 LRHAVSYTLSQDEAIVVEFGNDPETDMYQIGRCTEDQIDFVV 425
+H++S+TLS+ +++VE+ +D + DM+QIGR TE+ IDFVV
Sbjct: 100 GQHSISFTLSRSHSVIVEYTHDSDKDMFQIGRSTENMIDFVV 141
>sp|Q8N2H9|PELI3_HUMAN Pellino protein homolog 3 (Pellino 3)
Length = 469
Score = 93.2 bits (230), Expect = 7e-19
Identities = 46/95 (48%), Positives = 56/95 (58%)
Frame = +2
Query: 479 TVSRFACRIFINRKPPHTARIYAAGFDGNKNIFLGENAIKWEADKKIYDGFTTNGVMICH 658
T+SR+ACRI +R+PP+TARIYAAGFD + NIFLGE A KW + DG TTNGV++ H
Sbjct: 183 TISRYACRILCDRRPPYTARIYAAGFDASSNIFLGERAAKWRTPDGLMDGLTTNGVLVMH 242
Query: 659 PHVNFDPDIRSPDALLKTKECQSSFANSSWREVSV 763
P F D A WRE+SV
Sbjct: 243 PAGGFSED----------------SAPGVWREISV 261
Score = 70.9 bits (172), Expect = 4e-12
Identities = 32/82 (39%), Positives = 56/82 (68%)
Frame = +3
Query: 180 DNKKETNKLKVVQRSQSNGIKQIKIGRYKEAKSSQAVQSKLRHAVSYTLSQDEAIVVEFG 359
D + ++L + +RS +NG+K + S+A+ ++ +H++SYTLS+ +++VE+
Sbjct: 84 DKGRRRSRLALSRRSHANGVKPDVMHHISTPLVSKALSNRGQHSISYTLSRSHSVIVEYT 143
Query: 360 NDPETDMYQIGRCTEDQIDFVV 425
+D +TDM+QIGR TE+ IDFVV
Sbjct: 144 HDSDTDMFQIGRSTENMIDFVV 165
>sp|Q8C669|PELI1_MOUSE Pellino protein homolog 1 (Pellino 1)
Length = 418
Score = 92.8 bits (229), Expect = 1e-18
Identities = 47/95 (49%), Positives = 54/95 (56%)
Frame = +2
Query: 479 TVSRFACRIFINRKPPHTARIYAAGFDGNKNIFLGENAIKWEADKKIYDGFTTNGVMICH 658
T+SRFACRI R PP TARIYAAGFD +KNIFLGE A KW+ DG TTNGV++ H
Sbjct: 133 TISRFACRIICERSPPFTARIYAAGFDSSKNIFLGEKAAKWKTSDGQMDGLTTNGVLVMH 192
Query: 659 PHVNFDPDIRSPDALLKTKECQSSFANSSWREVSV 763
P F D + WRE+SV
Sbjct: 193 PRNGFTEDSKP----------------GIWREISV 211
Score = 80.5 bits (197), Expect = 5e-15
Identities = 44/116 (37%), Positives = 74/116 (63%), Gaps = 1/116 (0%)
Frame = +3
Query: 120 IVYGYLIVLGTNGRKSWRYSDNKKETNKLKVVQRSQSNGIKQIKIGRYKEAKSSQAVQSK 299
+ YG LIVLG NG S D + ++ + +R ++NG+K + ++++A+ +K
Sbjct: 15 VKYGELIVLGYNG--SLPNGDRGRRKSRFALFKRPKANGVKPSTVHIACTPQAAKAISNK 72
Query: 300 LRHAVSYTLSQDEAIVVEFGNDPETDMYQIGRCTEDQIDFVV-NSVPLDKECKNTK 464
+H++SYTLS+ + +VVE+ +D TDM+QIGR TE IDFVV ++VP + +T+
Sbjct: 73 DQHSISYTLSRAQTVVVEYTHDSNTDMFQIGRSTESPIDFVVTDTVPGSQSNSDTQ 128
>sp|Q96FA3|PELI1_HUMAN Pellino protein homolog 1 (Pellino 1) (Pellino related
intracellular signaling molecule)
Length = 418
Score = 92.8 bits (229), Expect = 1e-18
Identities = 47/95 (49%), Positives = 54/95 (56%)
Frame = +2
Query: 479 TVSRFACRIFINRKPPHTARIYAAGFDGNKNIFLGENAIKWEADKKIYDGFTTNGVMICH 658
T+SRFACRI R PP TARIYAAGFD +KNIFLGE A KW+ DG TTNGV++ H
Sbjct: 133 TISRFACRIICERNPPFTARIYAAGFDSSKNIFLGEKAAKWKTSDGQMDGLTTNGVLVMH 192
Query: 659 PHVNFDPDIRSPDALLKTKECQSSFANSSWREVSV 763
P F D + WRE+SV
Sbjct: 193 PRNGFTEDSKP----------------GIWREISV 211
Score = 80.5 bits (197), Expect = 5e-15
Identities = 44/116 (37%), Positives = 74/116 (63%), Gaps = 1/116 (0%)
Frame = +3
Query: 120 IVYGYLIVLGTNGRKSWRYSDNKKETNKLKVVQRSQSNGIKQIKIGRYKEAKSSQAVQSK 299
+ YG LIVLG NG S D + ++ + +R ++NG+K + ++++A+ +K
Sbjct: 15 VKYGELIVLGYNG--SLPNGDRGRRKSRFALFKRPKANGVKPSTVHIACTPQAAKAISNK 72
Query: 300 LRHAVSYTLSQDEAIVVEFGNDPETDMYQIGRCTEDQIDFVV-NSVPLDKECKNTK 464
+H++SYTLS+ + +VVE+ +D TDM+QIGR TE IDFVV ++VP + +T+
Sbjct: 73 DQHSISYTLSRAQTVVVEYTHDSNTDMFQIGRSTESPIDFVVTDTVPGSQSNSDTQ 128
>sp|Q9HAT8|PELI2_HUMAN Pellino protein homolog 2 (Pellino 2)
Length = 420
Score = 88.6 bits (218), Expect = 2e-17
Identities = 47/95 (49%), Positives = 57/95 (60%)
Frame = +2
Query: 479 TVSRFACRIFINRKPPHTARIYAAGFDGNKNIFLGENAIKWEADKKIYDGFTTNGVMICH 658
T+SRFACRI +R P+TARI+AAGFD +KNIFLGE A KW+ DG TTNGV++ H
Sbjct: 135 TISRFACRIVCDRNEPYTARIFAAGFDSSKNIFLGEKAAKWKNPDGHMDGLTTNGVLVMH 194
Query: 659 PHVNFDPDIRSPDALLKTKECQSSFANSSWREVSV 763
P F T+E Q WRE+SV
Sbjct: 195 PRGGF------------TEESQPGV----WREISV 213
Score = 81.6 bits (200), Expect = 2e-15
Identities = 39/102 (38%), Positives = 67/102 (65%)
Frame = +3
Query: 120 IVYGYLIVLGTNGRKSWRYSDNKKETNKLKVVQRSQSNGIKQIKIGRYKEAKSSQAVQSK 299
+ YG L+VLG NG + D + ++ + +R ++NG+K + ++S+A+ K
Sbjct: 17 VKYGELVVLGYNG--ALPNGDRGRRKSRFALYKRPKANGVKPSTVHVISTPQASKAISCK 74
Query: 300 LRHAVSYTLSQDEAIVVEFGNDPETDMYQIGRCTEDQIDFVV 425
+H++SYTLS+++ +VVE+ +D +TDM+Q+GR TE IDFVV
Sbjct: 75 GQHSISYTLSRNQTVVVEYTHDKDTDMFQVGRSTESPIDFVV 116
>sp|Q8BST6|PELI2_MOUSE Pellino protein homolog 2 (Pellino 2)
Length = 419
Score = 88.6 bits (218), Expect = 2e-17
Identities = 47/95 (49%), Positives = 57/95 (60%)
Frame = +2
Query: 479 TVSRFACRIFINRKPPHTARIYAAGFDGNKNIFLGENAIKWEADKKIYDGFTTNGVMICH 658
T+SRFACRI +R P+TARI+AAGFD +KNIFLGE A KW+ DG TTNGV++ H
Sbjct: 134 TISRFACRIVCDRNEPYTARIFAAGFDSSKNIFLGEKAAKWKNPDGHMDGLTTNGVLVMH 193
Query: 659 PHVNFDPDIRSPDALLKTKECQSSFANSSWREVSV 763
P F T+E Q WRE+SV
Sbjct: 194 PQGGF------------TEESQPGV----WREISV 212
Score = 77.8 bits (190), Expect = 3e-14
Identities = 38/102 (37%), Positives = 65/102 (63%)
Frame = +3
Query: 120 IVYGYLIVLGTNGRKSWRYSDNKKETNKLKVVQRSQSNGIKQIKIGRYKEAKSSQAVQSK 299
+ Y L+VLG NG + D + ++ + +R+ ++G+K I ++S+A+ S+
Sbjct: 17 VKYRELVVLGYNG--ALPNGDRGRRKSRFALYKRTYASGVKPSTIHMVSTPQASKAISSR 74
Query: 300 LRHAVSYTLSQDEAIVVEFGNDPETDMYQIGRCTEDQIDFVV 425
H++SYTLS+ + +VVE+ +D +TDM+Q+GR TE IDFVV
Sbjct: 75 GHHSISYTLSRSQTVVVEYTHDKDTDMFQVGRSTESPIDFVV 116
>sp|O77237|PELI_DROME Pellino protein
Length = 424
Score = 85.1 bits (209), Expect = 2e-16
Identities = 46/95 (48%), Positives = 57/95 (60%)
Frame = +2
Query: 479 TVSRFACRIFINRKPPHTARIYAAGFDGNKNIFLGENAIKWEADKKIYDGFTTNGVMICH 658
T+SRFACRI +NR P ARI+AAGFD ++NIFLGE A KW+ + +I DG TTNGV+I H
Sbjct: 142 TISRFACRILVNRCEPAKARIFAAGFDSSRNIFLGEKATKWQDNVEI-DGLTTNGVLIMH 200
Query: 659 PHVNFDPDIRSPDALLKTKECQSSFANSSWREVSV 763
P +F C + WRE SV
Sbjct: 201 PKGSF---------------CGGNAKCGLWRECSV 220
Score = 81.3 bits (199), Expect = 3e-15
Identities = 41/109 (37%), Positives = 74/109 (67%), Gaps = 1/109 (0%)
Frame = +3
Query: 126 YGYLIVLGTNGRKSWRYSDNKKETNKLKVVQRSQSNGIKQIKIGRYKEAKSSQAVQSKLR 305
YG L++LG NG D + +K + +R++++G+K+ K + ++S+A+ +
Sbjct: 28 YGELVILGYNGYLP--QGDRGRRRSKFVLHKRTEASGVKRSKHYIVQSPQTSKAILDANQ 85
Query: 306 HAVSYTLSQDEAIVVEFGNDPETDMYQIGRCTEDQIDFVV-NSVPLDKE 449
H++SYTLS+++A++VE+ D ETDM+Q+GR +E IDFVV +++P DK+
Sbjct: 86 HSISYTLSRNQAVIVEYKEDTETDMFQVGRSSESPIDFVVMDTLPGDKK 134
>sp|Q9XT84|IL2_DELLE Interleukin-2 precursor (IL-2) (T-cell growth factor) (TCGF)
Length = 154
Score = 35.8 bits (81), Expect = 0.14
Identities = 20/55 (36%), Positives = 24/55 (43%)
Frame = +3
Query: 384 QIGRCTEDQIDFVVNSVPLDKECKNTKLQVQKQFQDLHAEFSLIVNHLTLPEFML 548
Q+ C + V N P +NTK QVQ QDLH I NH L F +
Sbjct: 5 QLLSCIALTLALVANGAPTSSSTENTKKQVQSLLQDLHLLLKEINNHENLKLFRM 59
>sp|P46873|OSM3_CAEEL Osmotic avoidance abnormal protein 3 (Kinesin-like protein osm-3)
Length = 699
Score = 34.7 bits (78), Expect = 0.31
Identities = 30/118 (25%), Positives = 54/118 (45%), Gaps = 7/118 (5%)
Frame = +3
Query: 180 DNKKETNKLKVVQRSQSN-------GIKQIKIGRYKEAKSSQAVQSKLRHAVSYTLSQDE 338
DN K +Q+ Q G Q+K R K+ K ++ KL A + + +D+
Sbjct: 424 DNLNPEEAAKKIQQLQDQFIGGEEAGNTQLKQKRMKQLKEAETKTQKL--AAALNVHKDD 481
Query: 339 AIVVEFGNDPETDMYQIGRCTEDQIDFVVNSVPLDKECKNTKLQVQKQFQDLHAEFSL 512
++ Q+ T++++D V + L+KE K +K +++ +DLH EF L
Sbjct: 482 PLL------------QVYSTTQEKLDAVTSQ--LEKEVKKSK-GYEREIEDLHGEFEL 524
>sp|Q9PQF2|DNAK_UREPA Chaperone protein dnaK (Heat shock protein 70) (Heat shock 70 kDa
protein) (HSP70)
Length = 603
Score = 33.1 bits (74), Expect = 0.91
Identities = 21/72 (29%), Positives = 37/72 (51%)
Frame = +1
Query: 145 LVLMAVNHGGIQITKRKQIN*KLFKDLSQMV*NRLKSVDIKKLKVVKLFRVNLDMQLVIH 324
L +A++ G I K++N F +L++ + RLK + +K KL V++D L++
Sbjct: 255 LPFIAMDSSGQPINFEKELNRATFDNLTKNLIERLKKPVLDAMKESKLSLVDIDQVLMVG 314
Query: 325 YLKMKPLLSNLV 360
P + NLV
Sbjct: 315 GSTRMPAVQNLV 326
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 88,570,507
Number of Sequences: 369166
Number of extensions: 1900223
Number of successful extensions: 6118
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5945
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6116
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 7570361805
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)