Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_007_D18
(528 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9XI01|PDI1_ARATH Probable protein disulfide-isomerase 1... 73 4e-13
sp|Q9SRG3|PDI2_ARATH Probable protein disulfide-isomerase 2... 72 1e-12
sp|P05307|PDIA1_BOVIN Protein disulfide-isomerase precursor... 71 1e-12
sp|P52588|PDI_MAIZE Protein disulfide-isomerase precursor (... 71 1e-12
sp|P21195|PDIA1_RABIT Protein disulfide-isomerase precursor... 71 1e-12
sp|P54399|PDI_DROME Protein disulfide-isomerase precursor (... 71 2e-12
sp|P80284|PDI_HORVU Protein disulfide-isomerase precursor (... 71 2e-12
sp|O13811|PDI2_SCHPO Putative protein disulfide-isomerase C... 71 2e-12
sp|P07237|PDIA1_HUMAN Protein disulfide-isomerase precursor... 71 2e-12
sp|Q5R5B6|PDIA1_PONPY Protein disulfide-isomerase precursor... 71 2e-12
>sp|Q9XI01|PDI1_ARATH Probable protein disulfide-isomerase 1 precursor (PDI 1)
Length = 501
Score = 73.2 bits (178), Expect = 4e-13
Identities = 39/95 (41%), Positives = 54/95 (56%), Gaps = 3/95 (3%)
Frame = +2
Query: 23 VMFYSPWCGHCVAMKPALVNAANKLHNLLPSCLVAAVDATSETN--LAAQYKVTGFPTII 196
V FY+PWCGHC + P AA+ L + +P ++A +DA+ ETN A QY+V GFPTI
Sbjct: 52 VEFYAPWCGHCKQLAPEYEKAASALSSNVPPVVLAKIDASEETNREFATQYEVQGFPTIK 111
Query: 197 YFSPSGDWV-KYVGERTEDAIVQFMMKKDSDLKYE 298
F G V +Y G R + IV ++ K+ E
Sbjct: 112 IFRNGGKAVQEYNGpreAEGIVTYLKKQSGPASAE 146
Score = 63.5 bits (153), Expect = 3e-10
Identities = 36/100 (36%), Positives = 54/100 (54%), Gaps = 4/100 (4%)
Frame = +2
Query: 29 FYSPWCGHCVAMKPALVNAANKLHNLLPSCLVAAVDATSETNLAAQYKVTGFPTIIYFSP 208
FY+PWCGHC + P L A + S ++A +DAT+ + V GFPTI + S
Sbjct: 399 FYAPWCGHCQKLAPILDEVAVSYQSD-SSVVIAKLDATANDFPKDTFDVKGFPTIYFKSA 457
Query: 209 SGDWVKYVGERTEDAIVQFMMKKDSDL----KYEETQRKL 316
SG+ V Y G+RT++ + F+ K + K EET ++
Sbjct: 458 SGNVVVYEGDRTKEDFISFVDKNKDTVGEPKKEEETTEEV 497
>sp|Q9SRG3|PDI2_ARATH Probable protein disulfide-isomerase 2 precursor (PDI 2)
Length = 508
Score = 71.6 bits (174), Expect = 1e-12
Identities = 40/97 (41%), Positives = 59/97 (60%), Gaps = 2/97 (2%)
Frame = +2
Query: 8 KSGSFVM--FYSPWCGHCVAMKPALVNAANKLHNLLPSCLVAAVDATSETNLAAQYKVTG 181
KSG V+ FY+PWCGHC + P L A N PS ++A +DAT+ + + V G
Sbjct: 388 KSGKNVLIEFYAPWCGHCQKLAPILDEVALSFQND-PSVIIAKLDATANDIPSDTFDVKG 446
Query: 182 FPTIIYFSPSGDWVKYVGERTEDAIVQFMMKKDSDLK 292
FPTI + S SG+ V Y G+RT++ + F ++K+S+ K
Sbjct: 447 FPTIYFRSASGNVVVYEGDRTKEDFINF-VEKNSEKK 482
Score = 66.2 bits (160), Expect = 5e-11
Identities = 35/95 (36%), Positives = 51/95 (53%), Gaps = 3/95 (3%)
Frame = +2
Query: 23 VMFYSPWCGHCVAMKPALVNAANKLHNLLPSCLVAAVDATSETN--LAAQYKVTGFPTII 196
V FY+PWCGHC + P AA++L + P +A +DA+ E N A +YK+ GFPT+
Sbjct: 51 VEFYAPWCGHCQKLAPEYEKAASELSSHNPPLALAKIDASEEANKEFANEYKIQGFPTLK 110
Query: 197 YFSPSGDWVK-YVGERTEDAIVQFMMKKDSDLKYE 298
G V+ Y G R + IV ++ K+ E
Sbjct: 111 ILRNGGKSVQDYNGpreAEGIVTYLKKQSGPASVE 145
>sp|P05307|PDIA1_BOVIN Protein disulfide-isomerase precursor (PDI) (Prolyl 4-hydroxylase
beta subunit) (Cellular thyroid hormone binding protein)
(p55)
Length = 510
Score = 71.2 bits (173), Expect = 1e-12
Identities = 39/88 (44%), Positives = 51/88 (57%), Gaps = 3/88 (3%)
Frame = +2
Query: 23 VMFYSPWCGHCVAMKPALVNAANKLHNLLPSCLVAAVDATSETNLAAQYKVTGFPTIIYF 202
V FY+PWCGHC A+ P AA KL +A VDAT E++LA QY V G+PTI +F
Sbjct: 48 VEFYAPWCGHCKALAPEYAKAAGKLKAEGSEIRLAKVDATEESDLAQQYGVRGYPTIKFF 107
Query: 203 SPSGDWV---KYVGERTEDAIVQFMMKK 277
+GD +Y R D IV ++ K+
Sbjct: 108 K-NGDTASPKEYTAGREADDIVNWLKKR 134
Score = 55.8 bits (133), Expect = 6e-08
Identities = 32/96 (33%), Positives = 50/96 (52%), Gaps = 2/96 (2%)
Frame = +2
Query: 5 QKSGSFVMFYSPWCGHCVAMKPALVNAANKLHNLLPSCLVAAVDATSETNLAAQYKVTGF 184
+K FV FY+PWCGHC + P + + + + + ++A +D+T+ N KV F
Sbjct: 386 EKKNVFVEFYAPWCGHCKQLAP-IWDKLGETYKDHENIVIAKMDSTA--NEVEAVKVHSF 442
Query: 185 PTIIYFSPSGD--WVKYVGERTEDAIVQFMMKKDSD 286
PT+ +F S D + Y GERT D +F+ D
Sbjct: 443 PTLKFFPASADRTVIDYNGERTLDGFKKFLESGGQD 478
>sp|P52588|PDI_MAIZE Protein disulfide-isomerase precursor (PDI)
Length = 513
Score = 71.2 bits (173), Expect = 1e-12
Identities = 38/88 (43%), Positives = 50/88 (56%), Gaps = 3/88 (3%)
Frame = +2
Query: 23 VMFYSPWCGHCVAMKPALVNAANKLHNLLPSCLVAAVDATSETN--LAAQYKVTGFPTII 196
V FY+PWCGHC + P NAA L P ++A VDA E N LA +Y++ GFPTI
Sbjct: 56 VEFYAPWCGHCKKLAPEYENAAKALSKHDPPIVLAKVDANEEKNRPLATKYEIQGFPTIK 115
Query: 197 YFSPSGDWV-KYVGERTEDAIVQFMMKK 277
F G + +Y G R D IV ++ K+
Sbjct: 116 IFRDRGKNIQEYKGpreADGIVDYLKKQ 143
Score = 66.6 bits (161), Expect = 3e-11
Identities = 36/91 (39%), Positives = 54/91 (59%), Gaps = 2/91 (2%)
Frame = +2
Query: 8 KSGSFVM--FYSPWCGHCVAMKPALVNAANKLHNLLPSCLVAAVDATSETNLAAQYKVTG 181
KSG V+ FY+PWCGHC + P L AA L + ++A +DAT+ ++ +++ V G
Sbjct: 394 KSGKNVLIEFYAPWCGHCKKLAPILDEAATTLQS-DEEVVIAKMDATA-NDVPSEFDVQG 451
Query: 182 FPTIIYFSPSGDWVKYVGERTEDAIVQFMMK 274
+PT+ + +PSG Y RT D IV F+ K
Sbjct: 452 YPTLYFVTPSGKVTSYDSGRTADDIVDFIKK 482
>sp|P21195|PDIA1_RABIT Protein disulfide-isomerase precursor (PDI) (Prolyl 4-hydroxylase
beta subunit) (Cellular thyroid hormone binding protein)
(p55)
Length = 509
Score = 71.2 bits (173), Expect = 1e-12
Identities = 39/88 (44%), Positives = 51/88 (57%), Gaps = 3/88 (3%)
Frame = +2
Query: 23 VMFYSPWCGHCVAMKPALVNAANKLHNLLPSCLVAAVDATSETNLAAQYKVTGFPTIIYF 202
V FY+PWCGHC A+ P AA KL +A VDAT E++LA QY V G+PTI +F
Sbjct: 47 VEFYAPWCGHCKALAPEYAKAAGKLKAEGSDIRLAKVDATEESDLAQQYGVRGYPTIKFF 106
Query: 203 SPSGDWV---KYVGERTEDAIVQFMMKK 277
+GD +Y R D IV ++ K+
Sbjct: 107 K-NGDTASPKEYTAGREADDIVNWLKKR 133
Score = 54.3 bits (129), Expect = 2e-07
Identities = 31/96 (32%), Positives = 48/96 (50%), Gaps = 2/96 (2%)
Frame = +2
Query: 5 QKSGSFVMFYSPWCGHCVAMKPALVNAANKLHNLLPSCLVAAVDATSETNLAAQYKVTGF 184
+K FV FY+PWCGHC + P + + + + ++A +D+T+ N KV F
Sbjct: 385 EKKNVFVEFYAPWCGHCKQLAP-IWDKLGETYKEHQDIVIAKMDSTA--NEVEAVKVHSF 441
Query: 185 PTIIYF--SPSGDWVKYVGERTEDAIVQFMMKKDSD 286
PT+ +F P + Y GERT D +F+ D
Sbjct: 442 PTLKFFPAGPGRTVIDYNGERTLDGFKKFLESGGQD 477
>sp|P54399|PDI_DROME Protein disulfide-isomerase precursor (PDI)
Length = 496
Score = 70.9 bits (172), Expect = 2e-12
Identities = 38/85 (44%), Positives = 49/85 (57%)
Frame = +2
Query: 23 VMFYSPWCGHCVAMKPALVNAANKLHNLLPSCLVAAVDATSETNLAAQYKVTGFPTIIYF 202
V FY+PWCGHC A+ P AA +L +A VDAT E LA QY V G+PT+ +F
Sbjct: 49 VEFYAPWCGHCKALAPEYAKAAQQLAEKESPIKLAKVDATVEGELAEQYAVRGYPTLKFF 108
Query: 203 SPSGDWVKYVGERTEDAIVQFMMKK 277
SG V+Y G R I+ ++ KK
Sbjct: 109 R-SGSPVEYSGGRQAADIIAWVTKK 132
Score = 48.1 bits (113), Expect = 1e-05
Identities = 29/83 (34%), Positives = 44/83 (53%), Gaps = 1/83 (1%)
Frame = +2
Query: 23 VMFYSPWCGHCVAMKPALVNAANKLHNLLPSCLVAAVDATSETNLAAQYKVTGFPTIIYF 202
V FY+PWCGHC + P A K + ++A +D+T+ N K++ FPTI YF
Sbjct: 390 VEFYAPWCGHCKQLAPIYDQLAEKYKD-NEDIVIAKMDSTA--NELESIKISSFPTIKYF 446
Query: 203 SPSGDWV-KYVGERTEDAIVQFM 268
+ V + +RT D V+F+
Sbjct: 447 RKEDNKVIDFNLDRTLDDFVKFL 469
>sp|P80284|PDI_HORVU Protein disulfide-isomerase precursor (PDI) (Endosperm protein E-1)
Length = 513
Score = 70.9 bits (172), Expect = 2e-12
Identities = 38/91 (41%), Positives = 55/91 (60%), Gaps = 2/91 (2%)
Frame = +2
Query: 8 KSGSFVM--FYSPWCGHCVAMKPALVNAANKLHNLLPSCLVAAVDATSETNLAAQYKVTG 181
KSG V+ FY+PWCGHC + P L AA L + ++A +DAT E ++ ++ V G
Sbjct: 397 KSGKNVLIEFYAPWCGHCKKLAPILDEAAATLQSE-EDVVIAKMDAT-ENDVPGEFDVQG 454
Query: 182 FPTIIYFSPSGDWVKYVGERTEDAIVQFMMK 274
+PT+ + +PSG V Y G RT D IV ++ K
Sbjct: 455 YPTLYFVTPSGKKVSYEGGRTADEIVDYIRK 485
Score = 69.3 bits (168), Expect = 5e-12
Identities = 36/88 (40%), Positives = 52/88 (59%), Gaps = 3/88 (3%)
Frame = +2
Query: 23 VMFYSPWCGHCVAMKPALVNAANKLHNLLPSCLVAAVDATSETN--LAAQYKVTGFPTII 196
V FY+PWCGHC ++ P AA L P+ ++A VDA E N LA +Y+V GFPT+
Sbjct: 60 VEFYAPWCGHCKSLAPEYEKAAQLLSKHDPAIVLAKVDANDEKNKPLAGKYEVQGFPTLK 119
Query: 197 YFSPSGDWV-KYVGERTEDAIVQFMMKK 277
F G + +Y G R + IV+++ K+
Sbjct: 120 IFRNGGKSIQEYKGpreAEGIVEYLKKQ 147
>sp|O13811|PDI2_SCHPO Putative protein disulfide-isomerase C17H9.14c precursor
Length = 359
Score = 70.9 bits (172), Expect = 2e-12
Identities = 34/95 (35%), Positives = 58/95 (61%), Gaps = 3/95 (3%)
Frame = +2
Query: 2 SQKSGSFVMFYSPWCGHCVAMKPAL--VNAANKLHNLLPSCLVAAVDATSETNLAAQYKV 175
+ K G+ + FY+ WCGHC ++ P + A + HN L+ +DA + +++A +Y +
Sbjct: 37 ASKKGALIEFYATWCGHCKSLAPVYEELGALFEDHN---DVLIGKIDADTHSDVADKYHI 93
Query: 176 TGFPTIIYFSPSG-DWVKYVGERTEDAIVQFMMKK 277
TGFPT+I+F P G + V+Y R D++ QF+ +K
Sbjct: 94 TGFPTLIWFPPDGSEPVQYSNARDVDSLTQFVSEK 128
Score = 47.0 bits (110), Expect = 3e-05
Identities = 26/93 (27%), Positives = 48/93 (51%), Gaps = 3/93 (3%)
Frame = +2
Query: 8 KSGSFVMFYSPWCGHCVAMKPALVNAANKLHNLLPSCLVAAVDATSETNLAAQYKVTGFP 187
K V FY+ WCG+C + P K+ P+ + ++A ++ ++V FP
Sbjct: 158 KKDVLVEFYADWCGYCKRLAPT-YETLGKVFKNEPNVEIVKINADVFADIGRLHEVASFP 216
Query: 188 TIIYFSPSGDWVK---YVGERTEDAIVQFMMKK 277
TI +F P D K Y G+R+ +++++++ KK
Sbjct: 217 TIKFF-PKDDKDKPELYEGDRSLESLIEYINKK 248
>sp|P07237|PDIA1_HUMAN Protein disulfide-isomerase precursor (PDI) (Prolyl 4-hydroxylase
beta subunit) (Cellular thyroid hormone binding protein)
(p55)
Length = 508
Score = 70.9 bits (172), Expect = 2e-12
Identities = 39/88 (44%), Positives = 51/88 (57%), Gaps = 3/88 (3%)
Frame = +2
Query: 23 VMFYSPWCGHCVAMKPALVNAANKLHNLLPSCLVAAVDATSETNLAAQYKVTGFPTIIYF 202
V FY+PWCGHC A+ P AA KL +A VDAT E++LA QY V G+PTI +F
Sbjct: 46 VEFYAPWCGHCKALAPEYAKAAGKLKAEGSEIRLAKVDATEESDLAQQYGVRGYPTIKFF 105
Query: 203 SPSGDWV---KYVGERTEDAIVQFMMKK 277
+GD +Y R D IV ++ K+
Sbjct: 106 R-NGDTASPKEYTAGREADDIVNWLKKR 132
Score = 55.8 bits (133), Expect = 6e-08
Identities = 32/96 (33%), Positives = 50/96 (52%), Gaps = 2/96 (2%)
Frame = +2
Query: 5 QKSGSFVMFYSPWCGHCVAMKPALVNAANKLHNLLPSCLVAAVDATSETNLAAQYKVTGF 184
+K FV FY+PWCGHC + P + + + + + ++A +D+T+ N KV F
Sbjct: 384 EKKNVFVEFYAPWCGHCKQLAP-IWDKLGETYKDHENIVIAKMDSTA--NEVEAVKVHSF 440
Query: 185 PTIIYFSPSGD--WVKYVGERTEDAIVQFMMKKDSD 286
PT+ +F S D + Y GERT D +F+ D
Sbjct: 441 PTLKFFPASADRTVIDYNGERTLDGFKKFLESGGQD 476
>sp|Q5R5B6|PDIA1_PONPY Protein disulfide-isomerase precursor (PDI) (Prolyl 4-hydroxylase
beta subunit)
Length = 508
Score = 70.9 bits (172), Expect = 2e-12
Identities = 39/88 (44%), Positives = 51/88 (57%), Gaps = 3/88 (3%)
Frame = +2
Query: 23 VMFYSPWCGHCVAMKPALVNAANKLHNLLPSCLVAAVDATSETNLAAQYKVTGFPTIIYF 202
V FY+PWCGHC A+ P AA KL +A VDAT E++LA QY V G+PTI +F
Sbjct: 46 VEFYAPWCGHCKALAPEYAKAAGKLKAEGSEIRLAKVDATEESDLAQQYGVRGYPTIKFF 105
Query: 203 SPSGDWV---KYVGERTEDAIVQFMMKK 277
+GD +Y R D IV ++ K+
Sbjct: 106 R-NGDTASPKEYTAGREADDIVNWLKKR 132
Score = 55.8 bits (133), Expect = 6e-08
Identities = 32/96 (33%), Positives = 50/96 (52%), Gaps = 2/96 (2%)
Frame = +2
Query: 5 QKSGSFVMFYSPWCGHCVAMKPALVNAANKLHNLLPSCLVAAVDATSETNLAAQYKVTGF 184
+K FV FY+PWCGHC + P + + + + + ++A +D+T+ N KV F
Sbjct: 384 EKKNVFVEFYAPWCGHCKQLAP-IWDKLGETYKDHENIVIAKMDSTA--NEVEAVKVHSF 440
Query: 185 PTIIYFSPSGD--WVKYVGERTEDAIVQFMMKKDSD 286
PT+ +F S D + Y GERT D +F+ D
Sbjct: 441 PTLKFFPASADRTVIDYNGERTLDGFKKFLESGGQD 476
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 36,165,249
Number of Sequences: 369166
Number of extensions: 644354
Number of successful extensions: 1888
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1704
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1786
length of database: 68,354,980
effective HSP length: 103
effective length of database: 49,327,275
effective search space used: 3551563800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)