Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_007_D08
(447 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q19683|YZR5_CAEEL Hypothetical protein F21D5.5 in chromo... 85 6e-17
sp|Q96T60|PNKP_HUMAN Bifunctional polynucleotide phosphatas... 55 9e-08
sp|Q9JLV6|PNKP_MOUSE Bifunctional polynucleotide phosphatas... 55 9e-08
sp|P56089|GLYA_HELPY Serine hydroxymethyltransferase (Serin... 30 1.9
sp|Q7UFH9|SYA_RHOBA Alanyl-tRNA synthetase (Alanine--tRNA l... 30 3.2
sp|Q9ZMP7|GLYA_HELPJ Serine hydroxymethyltransferase (Serin... 30 3.2
sp|Q8TYA2|TRPA_METKA Tryptophan synthase alpha chain 29 4.1
sp|P48417|CP74_LINUS Cytochrome P450 74A, chloroplast precu... 28 7.1
sp|Q9P1Q0|VPS54_HUMAN Vacuolar protein sorting protein 54 (... 28 9.2
sp|O00410|IMB3_HUMAN Importin beta-3 (Karyopherin beta-3) (... 28 9.2
>sp|Q19683|YZR5_CAEEL Hypothetical protein F21D5.5 in chromosome IV
Length = 407
Score = 85.1 bits (209), Expect = 6e-17
Identities = 45/100 (45%), Positives = 64/100 (64%)
Frame = +1
Query: 31 TNPDLATRDRYISAAVECGVKHIICCIMDVSEDQSRHNEHFRQLTADHHDHVSEMVFKIH 210
T+PDL +R RYI A E GV I C M+ S + ++HN FR LT D+ +S MV +IH
Sbjct: 309 TSPDLESRKRYIDVAKELGVP-IRCFEMNCSMEHAQHNIRFRVLTDDNAAEISSMVLRIH 367
Query: 211 KNKYVEPSDKEKFTEICKIVPALEFENDDHKCIYSKYLLE 330
K KYVEP+ E F++I K+ +FE ++H+ +Y YL+E
Sbjct: 368 KGKYVEPTLSEGFSQIVKVNFRPKFEVEEHEKLYKMYLIE 407
>sp|Q96T60|PNKP_HUMAN Bifunctional polynucleotide phosphatase/kinase (Polynucleotide
kinase-3'-phosphatase) (DNA 5'-kinase/3'-phosphatase)
[Includes: Polynucleotide 3'-phosphatase
(2'(3')-polynucleotidase); Polynucleotide
5'-hydroxyl-kinase ]
Length = 521
Score = 54.7 bits (130), Expect = 9e-08
Identities = 29/79 (36%), Positives = 45/79 (56%)
Frame = +1
Query: 31 TNPDLATRDRYISAAVECGVKHIICCIMDVSEDQSRHNEHFRQLTADHHDHVSEMVFKIH 210
TNPD A+R RY+ A GV C + + +Q+RHN FR++T H VS+MV +
Sbjct: 424 TNPDAASRARYVQCARAAGVP-CRCFLFTATLEQARHNNRFREMTDSSHIPVSDMVMYGY 482
Query: 211 KNKYVEPSDKEKFTEICKI 267
+ ++ P+ E F+ I +I
Sbjct: 483 RKQFEAPTLAEGFSAILEI 501
>sp|Q9JLV6|PNKP_MOUSE Bifunctional polynucleotide phosphatase/kinase (Polynucleotide
kinase-3'-phosphatase) (DNA 5'-kinase/3'-phosphatase)
[Includes: Polynucleotide 3'-phosphatase
(2'(3')-polynucleotidase); Polynucleotide
5'-hydroxyl-kinase ]
Length = 522
Score = 54.7 bits (130), Expect = 9e-08
Identities = 30/79 (37%), Positives = 45/79 (56%)
Frame = +1
Query: 31 TNPDLATRDRYISAAVECGVKHIICCIMDVSEDQSRHNEHFRQLTADHHDHVSEMVFKIH 210
TNPD+ +R RYI A + GV C + +Q+RHN FR++T H VS+MV +
Sbjct: 423 TNPDVPSRARYIQCAKDAGVP-CRCFNFCATIEQARHNNRFREMTDPSHAPVSDMVMFSY 481
Query: 211 KNKYVEPSDKEKFTEICKI 267
+ ++ P+ E F EI +I
Sbjct: 482 RKQFEPPTLAEGFLEILEI 500
>sp|P56089|GLYA_HELPY Serine hydroxymethyltransferase (Serine methylase) (SHMT)
Length = 416
Score = 30.4 bits (67), Expect = 1.9
Identities = 14/57 (24%), Positives = 29/57 (50%), Gaps = 2/57 (3%)
Frame = +1
Query: 118 VSEDQSRHNEHFRQLTADHHDHVS--EMVFKIHKNKYVEPSDKEKFTEICKIVPALE 282
+ E+ R NEH + ++++ S E + + NKY E +++ C++V +E
Sbjct: 16 IFEEYKRQNEHLEMIASENYTFASVMEAMGSVLTNKYAEGYPNKRYYGGCEVVDKIE 72
>sp|Q7UFH9|SYA_RHOBA Alanyl-tRNA synthetase (Alanine--tRNA ligase) (AlaRS)
Length = 945
Score = 29.6 bits (65), Expect = 3.2
Identities = 18/57 (31%), Positives = 27/57 (47%)
Frame = +1
Query: 67 SAAVECGVKHIICCIMDVSEDQSRHNEHFRQLTADHHDHVSEMVFKIHKNKYVEPSD 237
+A+ CGVK+ E +S + R++T DH VF +H+N Y P D
Sbjct: 246 AASEVCGVKY---------EYESDNGRRLRRIT----DHARASVFAVHENVYPGPKD 289
>sp|Q9ZMP7|GLYA_HELPJ Serine hydroxymethyltransferase (Serine methylase) (SHMT)
Length = 416
Score = 29.6 bits (65), Expect = 3.2
Identities = 15/57 (26%), Positives = 29/57 (50%), Gaps = 2/57 (3%)
Frame = +1
Query: 118 VSEDQSRHNEHFRQLTADHHDH--VSEMVFKIHKNKYVEPSDKEKFTEICKIVPALE 282
+ E+ R NEH + ++++ V E + I NKY E +++ C++V +E
Sbjct: 16 IVEEFKRQNEHLEMIASENYTFPSVMEAMGSILTNKYAEGYPNKRYYGGCEVVDKIE 72
>sp|Q8TYA2|TRPA_METKA Tryptophan synthase alpha chain
Length = 275
Score = 29.3 bits (64), Expect = 4.1
Identities = 14/30 (46%), Positives = 17/30 (56%)
Frame = +1
Query: 55 DRYISAAVECGVKHIICCIMDVSEDQSRHN 144
+RY+SAA E GV II M V E H+
Sbjct: 111 ERYLSAAAEAGVSGIIVADMPVEESDEVHS 140
>sp|P48417|CP74_LINUS Cytochrome P450 74A, chloroplast precursor (Allene oxide synthase)
(Hydroperoxide dehydrase)
Length = 536
Score = 28.5 bits (62), Expect = 7.1
Identities = 11/35 (31%), Positives = 22/35 (62%), Gaps = 2/35 (5%)
Frame = +1
Query: 172 HHDHVSEMVFKIHKNK--YVEPSDKEKFTEICKIV 270
+H + +++F + KN+ YV P FT++C++V
Sbjct: 181 NHTKLKQLLFNLIKNRRDYVIPEFSSSFTDLCEVV 215
>sp|Q9P1Q0|VPS54_HUMAN Vacuolar protein sorting protein 54 (Tumor antigen SLP-8p)
(Hepatocellular carcinoma protein 8)
Length = 977
Score = 28.1 bits (61), Expect = 9.2
Identities = 9/20 (45%), Positives = 15/20 (75%)
Frame = +1
Query: 148 HFRQLTADHHDHVSEMVFKI 207
HF +T D+HDH++E+ K+
Sbjct: 842 HFDHITKDYHDHIAEISAKL 861
>sp|O00410|IMB3_HUMAN Importin beta-3 (Karyopherin beta-3) (Ran-binding protein 5) (RanBP5)
Length = 1097
Score = 28.1 bits (61), Expect = 9.2
Identities = 24/91 (26%), Positives = 45/91 (49%)
Frame = +1
Query: 88 VKHIICCIMDVSEDQSRHNEHFRQLTADHHDHVSEMVFKIHKNKYVEPSDKEKFTEICKI 267
+ H ++V D +NEHF +L + E FK + + V+ D E + E ++
Sbjct: 769 IMHSFAKCIEVMGDGCLNNEHFEELGGILKAKLEEH-FKNQELRQVKRQD-EDYDE--QV 824
Query: 268 VPALEFENDDHKCIYSKYLLEK*ADVVHRIY 360
+L+ E+D+ Y+L K +D++H I+
Sbjct: 825 EESLQDEDDN-----DVYILTKVSDILHSIF 850
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 49,875,848
Number of Sequences: 369166
Number of extensions: 919504
Number of successful extensions: 2292
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2259
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2289
length of database: 68,354,980
effective HSP length: 100
effective length of database: 49,881,480
effective search space used: 2394311040
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)