Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_007_C14
(889 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O00418|EF2K_HUMAN Elongation factor 2 kinase (eEF-2 kina... 72 3e-12
sp|P70531|EF2K_RAT Elongation factor 2 kinase (eEF-2 kinase... 71 5e-12
sp|O08796|EF2K_MOUSE Elongation factor 2 kinase (eEF-2 kina... 70 6e-12
sp|O01991|EF2K_CAEEL Elongation factor 2 kinase (eEF-2 kina... 49 1e-05
>sp|O00418|EF2K_HUMAN Elongation factor 2 kinase (eEF-2 kinase) (eEF-2K)
(Calcium/calmodulin-dependent eukaryotic elongation
factor 2 kinase)
Length = 725
Score = 71.6 bits (174), Expect = 3e-12
Identities = 45/138 (32%), Positives = 66/138 (47%)
Frame = +1
Query: 415 NLDQEIGHSILAQVHHELARLHESGRFIKSKQLAAGCLHHQFWSHLLNENDSPEAIVKET 594
+L+++IG SIL +VH + R HE GRF C + W
Sbjct: 513 DLEKKIGKSILGKVHLAMVRYHEGGRF---------CEKGEEWDQ--------------- 548
Query: 595 SNDINWESCLFHERHAAQLGCLEAVIVMARYCLNMSGHMLQDCPFPPTQNDLDCGLDYLW 774
ES +FH HAA LG LEA++ + + H+L D T+ + G DYL
Sbjct: 549 ------ESAVFHLEHAANLGELEAIVGLGLMYSQLPHHILADVSLKETEENKTKGFDYLL 602
Query: 775 RAADSGDRRSMIELANLY 828
+AA++GDR+SMI +A +
Sbjct: 603 KAAEAGDRQSMILVARAF 620
>sp|P70531|EF2K_RAT Elongation factor 2 kinase (eEF-2 kinase) (eEF-2K)
(Calcium/calmodulin-dependent eukaryotic elongation
factor 2 kinase)
Length = 724
Score = 70.9 bits (172), Expect = 5e-12
Identities = 44/138 (31%), Positives = 65/138 (47%)
Frame = +1
Query: 415 NLDQEIGHSILAQVHHELARLHESGRFIKSKQLAAGCLHHQFWSHLLNENDSPEAIVKET 594
+L ++IG S+L +VH + R HE GRF C + W
Sbjct: 512 DLGRKIGKSVLGKVHLAMVRYHEGGRF---------CEKDEEWDQ--------------- 547
Query: 595 SNDINWESCLFHERHAAQLGCLEAVIVMARYCLNMSGHMLQDCPFPPTQNDLDCGLDYLW 774
ES +FH HAA LG LEA++ + + H+L D T+ + G DYL
Sbjct: 548 ------ESAIFHLEHAADLGELEAIVGLGLMYSQLPHHILADVSLEETEENKTKGFDYLL 601
Query: 775 RAADSGDRRSMIELANLY 828
+AA++GDR+SMI +A +
Sbjct: 602 KAAEAGDRQSMILVARAF 619
>sp|O08796|EF2K_MOUSE Elongation factor 2 kinase (eEF-2 kinase) (eEF-2K)
(Calcium/calmodulin-dependent eukaryotic elongation
factor 2 kinase)
Length = 724
Score = 70.5 bits (171), Expect = 6e-12
Identities = 44/138 (31%), Positives = 64/138 (46%)
Frame = +1
Query: 415 NLDQEIGHSILAQVHHELARLHESGRFIKSKQLAAGCLHHQFWSHLLNENDSPEAIVKET 594
+L ++IG S+L +VH + R HE GRF C + W
Sbjct: 512 DLGRKIGKSVLGKVHLAMVRYHEGGRF---------CEKDEEWDR--------------- 547
Query: 595 SNDINWESCLFHERHAAQLGCLEAVIVMARYCLNMSGHMLQDCPFPPTQNDLDCGLDYLW 774
ES +FH HAA LG LEA++ + + H+L D T+ + G DYL
Sbjct: 548 ------ESAIFHLEHAADLGELEAIVGLGLMYSQLPHHILADVSLKETEENKTKGFDYLL 601
Query: 775 RAADSGDRRSMIELANLY 828
+AA++GDR SMI +A +
Sbjct: 602 KAAEAGDRHSMILVARAF 619
>sp|O01991|EF2K_CAEEL Elongation factor 2 kinase (eEF-2 kinase) (eEF-2K)
(Calcium/calmodulin-dependent eukaryotic elongation
factor 2 kinase)
Length = 768
Score = 49.3 bits (116), Expect = 1e-05
Identities = 45/179 (25%), Positives = 81/179 (45%), Gaps = 26/179 (14%)
Frame = +1
Query: 370 SMRENQKPSSATHPTNLDQEIGH--SILAQVHHELARLHESGRFI----KSKQLAAGCLH 531
S++ Q ++ + ++ Q GH S+L Q+H +L+R HE GRF+ + K++ G
Sbjct: 500 SLQLQQMAANLENDEDVPQVTGHQFSVLGQIHIDLSRYHELGRFVEVDSEHKEMLEG--- 556
Query: 532 HQFWSHLLNENDSPEAIVKETSNDINWESCLFHERHAAQLGCLEAVIVMARYCLNMSGHM 711
+END+ I + +S +FH A + G LEAV+ A L + +
Sbjct: 557 --------SENDARVPIKYDK------QSAIFHLDIARKCGILEAVLTSAHIVLGLPHEL 602
Query: 712 LQDCP--------FPPTQNDL------------DCGLDYLWRAADSGDRRSMIELANLY 828
L++ F +N + + G D + AA+ GD+ +M+ +A+ Y
Sbjct: 603 LKEVTVDDLFPNGFGEQENGIRADKGQKPCDLEEFGSDLMEIAAEMGDKGAMLYMAHAY 661
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 93,577,145
Number of Sequences: 369166
Number of extensions: 1778355
Number of successful extensions: 3939
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 3798
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3937
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 8886314050
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)