Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_007_A12
(917 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P70498|PLD2_RAT Phospholipase D2 (PLD 2) (Choline phosph... 34 0.66
sp|P97813|PLD2_MOUSE Phospholipase D2 (PLD 2) (Choline phos... 33 0.86
sp|P14222|PERF_HUMAN Perforin 1 precursor (P1) (Lymphocyte ... 32 3.3
sp|O14939|PLD2_HUMAN Phospholipase D2 (PLD 2) (Choline phos... 32 3.3
sp|P10820|PERF_MOUSE Perforin 1 precursor (P1) (Lymphocyte ... 31 4.3
sp|O88281|EGFL3_RAT Multiple EGF-like-domain protein 3 prec... 31 4.3
sp|O75095|EGFL3_HUMAN Multiple EGF-like-domain protein 3 pr... 31 5.6
sp|Q02858|TIE2_MOUSE Angiopoietin-1 receptor precursor (Tyr... 30 7.3
sp|Q66D26|PHNC_YERPS Phosphonates import ATP-binding protei... 30 7.3
sp|Q80V70|EGFL3_MOUSE Multiple EGF-like-domain protein 3 30 7.3
>sp|P70498|PLD2_RAT Phospholipase D2 (PLD 2) (Choline phosphatase 2)
(Phosphatidylcholine-hydrolyzing phospholipase D2)
(PLD1C) (rPLD2)
Length = 933
Score = 33.9 bits (76), Expect = 0.66
Identities = 25/96 (26%), Positives = 43/96 (44%), Gaps = 5/96 (5%)
Frame = +1
Query: 445 SFGDWEMSIMDNLVPIDRRGEPLYYVITRHALPDLDDGTVYKINDLLREAIERYYKINER 624
S G E SI++ + R + Y+ + + D TV +N + E ++R K +E+
Sbjct: 614 SAGTLESSILNAYLHTIRESQHFLYIENQFFISCSDGRTV--LNKVGDEIVDRILKAHEQ 671
Query: 625 KGCLDF-----TSPNFDPDANVDGGNCEHPLLNFTF 717
C P F+ D + GGN +L+FT+
Sbjct: 672 GQCFRVYVLLPLLPGFEGDISTGGGNSIQAILHFTY 707
>sp|P97813|PLD2_MOUSE Phospholipase D2 (PLD 2) (Choline phosphatase 2)
(Phosphatidylcholine-hydrolyzing phospholipase D2)
(PLD1C) (mPLD2)
Length = 933
Score = 33.5 bits (75), Expect = 0.86
Identities = 25/96 (26%), Positives = 43/96 (44%), Gaps = 5/96 (5%)
Frame = +1
Query: 445 SFGDWEMSIMDNLVPIDRRGEPLYYVITRHALPDLDDGTVYKINDLLREAIERYYKINER 624
S G E SI++ + R + Y+ + + D TV +N + E ++R K +E+
Sbjct: 614 SAGTLENSILNAYLHTIRESQHFLYIENQFFISCSDGRTV--LNKVGDEIVDRILKAHEQ 671
Query: 625 KGCLDF-----TSPNFDPDANVDGGNCEHPLLNFTF 717
C P F+ D + GGN +L+FT+
Sbjct: 672 GQCFRVYLLLPLLPGFEGDISTGGGNSIQAILHFTY 707
>sp|P14222|PERF_HUMAN Perforin 1 precursor (P1) (Lymphocyte pore forming protein) (PFP)
(Cytolysin)
Length = 555
Score = 31.6 bits (70), Expect = 3.3
Identities = 47/213 (22%), Positives = 75/213 (35%), Gaps = 20/213 (9%)
Frame = +1
Query: 28 VQLRYNIFQSMFEPEAPLSSTFKNRIMDIAGLIVENKDSLYLTYLADMIIRDYGTHVIRS 207
V+ R+ F + P PL FK + D+ + YL +I +YGTH IR+
Sbjct: 174 VECRFYSFHVVHTP--PLHPDFKRALGDLPHHFNASTQPAYLR-----LISNYGTHFIRA 226
Query: 208 IDAGAILYKTDVLKSTYVKNVYSNMNAISVXXXXXXXXXXNIGAKYSTQRTQNELDEYNK 387
++ G + L++ + + V NIG S +E K
Sbjct: 227 VELGGRISALTALRTCEL--ALEGLTDNEVEDCLTVEAQVNIGIHGSISAEAKACEEKKK 284
Query: 388 NVDTT----------HIHRIGG-------AIFGVNS---SFGDWEMSIMDNLVPIDRRGE 507
T H +GG +FG+ + + W S+ + +D E
Sbjct: 285 KHKMTASFHQTYRERHSEVVGGHHTSINDLLFGIQAGPEQYSAWVNSLPGSPGLVDYTLE 344
Query: 508 PLYYVITRHALPDLDDGTVYKINDLLREAIERY 606
PL H L D D + LR A+ +Y
Sbjct: 345 PL------HVLLDSQDPR----REALRRALSQY 367
>sp|O14939|PLD2_HUMAN Phospholipase D2 (PLD 2) (Choline phosphatase 2)
(Phosphatidylcholine-hydrolyzing phospholipase D2)
(PLD1C) (hPLD2)
Length = 933
Score = 31.6 bits (70), Expect = 3.3
Identities = 24/96 (25%), Positives = 43/96 (44%), Gaps = 5/96 (5%)
Frame = +1
Query: 445 SFGDWEMSIMDNLVPIDRRGEPLYYVITRHALPDLDDGTVYKINDLLREAIERYYKINER 624
S G E SI++ + R + Y+ + + D TV +N + E ++R K +++
Sbjct: 614 SAGTLENSILNAYLHTIRESQHFLYIENQFFISCSDGRTV--LNKVGDEIVDRILKAHKQ 671
Query: 625 KGCLDF-----TSPNFDPDANVDGGNCEHPLLNFTF 717
C P F+ D + GGN +L+FT+
Sbjct: 672 GWCYRVYVLLPLLPGFEGDISTGGGNSIQAILHFTY 707
>sp|P10820|PERF_MOUSE Perforin 1 precursor (P1) (Lymphocyte pore forming protein)
(Cytolysin)
Length = 554
Score = 31.2 bits (69), Expect = 4.3
Identities = 38/186 (20%), Positives = 71/186 (38%), Gaps = 20/186 (10%)
Frame = +1
Query: 28 VQLRYNIFQSMFEPEAPLSSTFKNRIMDIAGLIVENKDSLYLTYLADMIIRDYGTHVIRS 207
V+ R F+ + +P PL FK + + + + Y +I YGTH I +
Sbjct: 173 VECRMYSFRLVQKP--PLHLDFKKALRALPRNFNSSTEHAY-----HRLISSYGTHFITA 225
Query: 208 IDAGAILYKTDVLKSTYVKNVYSNMNAISVXXXXXXXXXXNIGAKYSTQRTQNELDE--- 378
+D G + L++ + + + A V +IGA+ S +E
Sbjct: 226 VDLGGRISVLTALRTCQL--TLNGLTADEVGDCLNVEAQVSIGAQASVSSEYKACEEKKK 283
Query: 379 -------YNKNVDTTHIHRIGG-------AIFGVNSS---FGDWEMSIMDNLVPIDRRGE 507
+++ H+ +GG +FG ++ F W S+ N +D E
Sbjct: 284 QHKMATSFHQTYRERHVEVLGGPLDSTHDLLFGNQATPEQFSTWTASLPSNPGLVDYSLE 343
Query: 508 PLYYVI 525
PL+ ++
Sbjct: 344 PLHTLL 349
>sp|O88281|EGFL3_RAT Multiple EGF-like-domain protein 3 precursor (Multiple epidermal
growth factor-like domains 6)
Length = 1574
Score = 31.2 bits (69), Expect = 4.3
Identities = 21/74 (28%), Positives = 31/74 (41%), Gaps = 1/74 (1%)
Frame = +1
Query: 679 GGNCEHPLLNFTFG-GIYQNCTGPLGPSYSQINPLTGSYSCPLGFSSVKLHSGLIQCDTS 855
G +CE FG Q C L P+ + + +TG CP GF+ + C+ +
Sbjct: 1159 GPHCEQACPRGWFGEACAQRC---LCPTNASCHHVTGECRCPPGFTG-------LSCEQA 1208
Query: 856 CSGWWFWKHCDTRC 897
C F K C+ C
Sbjct: 1209 CQPGTFGKDCEHLC 1222
Score = 30.8 bits (68), Expect = 5.6
Identities = 20/74 (27%), Positives = 31/74 (41%), Gaps = 1/74 (1%)
Frame = +1
Query: 679 GGNCEHPLLNFTFG-GIYQNCTGPLGPSYSQINPLTGSYSCPLGFSSVKLHSGLIQCDTS 855
G C+ ++ TFG ++C G S + TG+ CP G+ C+ +
Sbjct: 1116 GDKCQSSCVSGTFGVHCEEHCACRKGASCHHV---TGACFCPPGWRGP-------HCEQA 1165
Query: 856 CSGWWFWKHCDTRC 897
C WF + C RC
Sbjct: 1166 CPRGWFGEACAQRC 1179
>sp|O75095|EGFL3_HUMAN Multiple EGF-like-domain protein 3 precursor (Multiple epidermal
growth factor-like domains 6)
Length = 1229
Score = 30.8 bits (68), Expect = 5.6
Identities = 15/43 (34%), Positives = 20/43 (46%)
Frame = +1
Query: 772 NPLTGSYSCPLGFSSVKLHSGLIQCDTSCSGWWFWKHCDTRCS 900
+P TG CP G++ K C + C WF + C RCS
Sbjct: 907 DPHTGRCLCPAGWTGDK-------CQSPCLRGWFGEACAQRCS 942
>sp|Q02858|TIE2_MOUSE Angiopoietin-1 receptor precursor (Tyrosine-protein kinase receptor
TIE-2) (Tyrosine-protein kinase receptor TEK) (P140 TEK)
(Tunica interna endothelial cell kinase) (HYK) (STK1)
Length = 1122
Score = 30.4 bits (67), Expect = 7.3
Identities = 22/75 (29%), Positives = 30/75 (40%), Gaps = 2/75 (2%)
Frame = +1
Query: 679 GGNCEHPLLNFTFGGIY-QNCTGPLG-PSYSQINPLTGSYSCPLGFSSVKLHSGLIQCDT 852
G CE TFG + C+GP G SY P SC G+ +QC+
Sbjct: 248 GRTCEKACEPHTFGRTCKERCSGPEGCKSYVFCLPDPYGCSCATGWRG-------LQCNE 300
Query: 853 SCSGWWFWKHCDTRC 897
+C ++ C RC
Sbjct: 301 ACPSGYYGPDCKLRC 315
>sp|Q66D26|PHNC_YERPS Phosphonates import ATP-binding protein phnC
sp|Q8ZGU5|PHNC_YERPE Phosphonates import ATP-binding protein phnC
Length = 278
Score = 30.4 bits (67), Expect = 7.3
Identities = 22/77 (28%), Positives = 40/77 (51%), Gaps = 2/77 (2%)
Frame = +1
Query: 64 EPEAPLSSTFKNRIMDIAGLIVENKDSLYLTYLADMIIRDYGTHVIRSIDAGAILY--KT 237
EP A L RIM+ I EN ++ + + +++DY T VI I G I++
Sbjct: 199 EPVASLDPKNTTRIMNTLQKISENDIAVIVNLHSVDLVKDYCTRVI-GIAHGRIIFDGPP 257
Query: 238 DVLKSTYVKNVYSNMNA 288
+L + ++++YS+ +A
Sbjct: 258 SMLNDSIIQDIYSDESA 274
>sp|Q80V70|EGFL3_MOUSE Multiple EGF-like-domain protein 3
Length = 656
Score = 30.4 bits (67), Expect = 7.3
Identities = 19/74 (25%), Positives = 31/74 (41%), Gaps = 1/74 (1%)
Frame = +1
Query: 679 GGNCEHPLLNFTFG-GIYQNCTGPLGPSYSQINPLTGSYSCPLGFSSVKLHSGLIQCDTS 855
G C+ P ++ FG ++C G + + TG+ CP G+ C+ +
Sbjct: 198 GDKCQSPCVSGMFGVHCEEHCACRKGATCHHV---TGACLCPPGWRGS-------HCEQA 247
Query: 856 CSGWWFWKHCDTRC 897
C WF + C RC
Sbjct: 248 CPRGWFGEACAQRC 261
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 100,818,245
Number of Sequences: 369166
Number of extensions: 2083990
Number of successful extensions: 5188
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5007
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5184
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 9366655350
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)