Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_006_P09
(874 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O22042|M3K3_ARATH Mitogen-activated protein kinase kinas... 59 2e-08
sp|P27636|CDC15_YEAST Cell division control protein 15 59 2e-08
sp|O22040|M3K1_ARATH Mitogen-activated protein kinase kinas... 57 9e-08
sp|Q9FZ36|M3K2_ARATH Mitogen-activated protein kinase kinas... 55 3e-07
sp|Q9J4Z5|V245_FOWPV Putative ankyrin repeat protein FPV245 52 3e-06
sp|P53599|SSK2_YEAST MAP kinase kinase kinase SSK2 (Suppres... 52 3e-06
sp|Q9M8S6|SKOR_ARATH Potassium channel SKOR (Stelar K(+) ou... 51 5e-06
sp|Q9Y6E0|STK24_HUMAN Serine/threonine-protein kinase 24 (S... 49 2e-05
sp|P47810|WEE1_MOUSE Wee1-like protein kinase 49 2e-05
sp|Q99KH8|STK24_MOUSE Serine/threonine-protein kinase 24 49 2e-05
>sp|O22042|M3K3_ARATH Mitogen-activated protein kinase kinase kinase 3 (Arabidospsis
NPK1-related protein kinase 3)
Length = 651
Score = 58.9 bits (141), Expect = 2e-08
Identities = 30/108 (27%), Positives = 59/108 (54%)
Frame = +2
Query: 440 IEFDKSDVITCGRFAKVFKVVESKENRVFAVKKVTWEQEQADNFPEKENNLHEIKKDAKW 619
I + K ++I CG F +V+ + + A+K+V A + + ++ E++++ +
Sbjct: 66 IRWRKGELIGCGAFGRVYMGMNLDSGELLAIKQVLIAPSSASK-EKTQGHIRELEEEVQL 124
Query: 620 LENNINDYKTILSHPHIIKYFGAIKEDYKIFIFMEYAENGSIHNLIQK 763
L+N LSHP+I++Y G ++E + I ME+ GSI +L++K
Sbjct: 125 LKN--------LSHPNIVRYLGTVRESDSLNILMEFVPGGSISSLLEK 164
Score = 31.6 bits (70), Expect = 3.0
Identities = 12/21 (57%), Positives = 16/21 (76%)
Frame = +3
Query: 807 QHILLALDYLHGKNILHRDIK 869
+ +LL L+YLH I+HRDIK
Sbjct: 178 KQLLLGLEYLHNNGIMHRDIK 198
>sp|P27636|CDC15_YEAST Cell division control protein 15
Length = 974
Score = 58.5 bits (140), Expect = 2e-08
Identities = 30/109 (27%), Positives = 59/109 (54%)
Frame = +2
Query: 440 IEFDKSDVITCGRFAKVFKVVESKENRVFAVKKVTWEQEQADNFPEKENNLHEIKKDAKW 619
+++ VI G + V+K + ++V A+K+V +E ++ L++I +
Sbjct: 23 VQYHLKQVIGRGSYGVVYKAINKHTDQVVAIKEVVYENDE---------ELNDIMAEISL 73
Query: 620 LENNINDYKTILSHPHIIKYFGAIKEDYKIFIFMEYAENGSIHNLIQKA 766
L+N L+H +I+KY G I++ Y+++I +EY NGS+ LI ++
Sbjct: 74 LKN--------LNHNNIVKYHGFIRKSYELYILLEYCANGSLRRLISRS 114
Score = 31.6 bits (70), Expect = 3.0
Identities = 12/18 (66%), Positives = 15/18 (83%)
Frame = +3
Query: 816 LLALDYLHGKNILHRDIK 869
LL L YLHG+ ++HRDIK
Sbjct: 131 LLGLKYLHGEGVIHRDIK 148
>sp|O22040|M3K1_ARATH Mitogen-activated protein kinase kinase kinase 1 (Arabidospsis
NPK1-related protein kinase 1)
Length = 666
Score = 56.6 bits (135), Expect = 9e-08
Identities = 40/129 (31%), Positives = 65/129 (50%), Gaps = 3/129 (2%)
Frame = +2
Query: 440 IEFDKSDVITCGRFAKVFKVVESKENRVFAVKKVTWEQEQADNFPEKEN---NLHEIKKD 610
I + K +I G F V+ + + AVK+V A NF KE ++ E++++
Sbjct: 67 ISWRKGQLIGRGAFGTVYMGMNLDSGELLAVKQVLI----AANFASKEKTQAHIQELEEE 122
Query: 611 AKWLENNINDYKTILSHPHIIKYFGAIKEDYKIFIFMEYAENGSIHNLIQKAIKVAFHTI 790
K L+N LSHP+I++Y G ++ED + I +E+ GSI +L++K F
Sbjct: 123 VKLLKN--------LSHPNIVRYLGTVREDDTLNILLEFVPGGSISSLLEK-----FGPF 169
Query: 791 ILSNIATYT 817
S + TYT
Sbjct: 170 PESVVRTYT 178
Score = 30.4 bits (67), Expect = 6.7
Identities = 12/21 (57%), Positives = 16/21 (76%)
Frame = +3
Query: 807 QHILLALDYLHGKNILHRDIK 869
+ +LL L+YLH I+HRDIK
Sbjct: 179 RQLLLGLEYLHNHAIMHRDIK 199
>sp|Q9FZ36|M3K2_ARATH Mitogen-activated protein kinase kinase kinase 2 (Arabidospsis
NPK1-related protein kinase 2)
Length = 651
Score = 54.7 bits (130), Expect = 3e-07
Identities = 36/126 (28%), Positives = 63/126 (50%)
Frame = +2
Query: 440 IEFDKSDVITCGRFAKVFKVVESKENRVFAVKKVTWEQEQADNFPEKENNLHEIKKDAKW 619
I + K +I G F V+ + + AVK+V A + + ++ E++++ K
Sbjct: 66 IRWRKGQLIGRGAFGTVYMGMNLDSGELLAVKQVLITSNCASK-EKTQAHIQELEEEVKL 124
Query: 620 LENNINDYKTILSHPHIIKYFGAIKEDYKIFIFMEYAENGSIHNLIQKAIKVAFHTIILS 799
L+N LSHP+I++Y G ++ED + I +E+ GSI +L++K F S
Sbjct: 125 LKN--------LSHPNIVRYLGTVREDETLNILLEFVPGGSISSLLEK-----FGAFPES 171
Query: 800 NIATYT 817
+ TYT
Sbjct: 172 VVRTYT 177
Score = 30.0 bits (66), Expect = 8.8
Identities = 12/19 (63%), Positives = 15/19 (78%)
Frame = +3
Query: 813 ILLALDYLHGKNILHRDIK 869
+LL L+YLH I+HRDIK
Sbjct: 180 LLLGLEYLHNHAIMHRDIK 198
>sp|Q9J4Z5|V245_FOWPV Putative ankyrin repeat protein FPV245
Length = 436
Score = 51.6 bits (122), Expect = 3e-06
Identities = 35/121 (28%), Positives = 59/121 (48%), Gaps = 3/121 (2%)
Frame = +2
Query: 17 AVKSNNLVDVERKFN-SEFLNKPENEGYYPIHLAGINNAVDVFKYFLQQNCDIELLDSRR 193
A+K+N+L ++ F +N ++ G YPIH+A +N+ ++ K L++ + D+
Sbjct: 131 AIKNNDLEVIKMLFEYGADVNIKDDNGCYPIHIATRSNSYEIIKLLLEKGAYANVKDNYG 190
Query: 194 NRPIYHAIKNGSEEIVKLLLGHRCSFDN--GGALLELAKTANKNREKTIEIVEKAIENRQ 367
N P+++A K G +KL+L H + N + L NR VE I NR
Sbjct: 191 NSPLHNAAKYGDYACIKLVLDHTNNISNKCNNGVTPLHNAILYNR----SAVELLINNRS 246
Query: 368 I 370
I
Sbjct: 247 I 247
Score = 36.6 bits (83), Expect = 0.094
Identities = 18/50 (36%), Positives = 28/50 (56%)
Frame = +2
Query: 104 IHLAGINNAVDVFKYFLQQNCDIELLDSRRNRPIYHAIKNGSEEIVKLLL 253
+H A NN ++V K + D+ + D PI+ A ++ S EI+KLLL
Sbjct: 128 LHYAIKNNDLEVIKMLFEYGADVNIKDDNGCYPIHIATRSNSYEIIKLLL 177
Score = 36.6 bits (83), Expect = 0.094
Identities = 24/84 (28%), Positives = 39/84 (46%), Gaps = 2/84 (2%)
Frame = +2
Query: 44 VERKFNSEFLNKPENEGYYPIHLA-GINNAVDVFKYFLQQNCDIELLDSRRNRPIYHAIK 220
VE N+ +N + +GY P+H A ++D+ L N DI + D+ PI A K
Sbjct: 238 VELLINNRSINDTDVDGYTPLHYALQPPCSIDIIDILLYNNADISIKDNNGRNPIDTAFK 297
Query: 221 N-GSEEIVKLLLGHRCSFDNGGAL 289
+ ++K LL + + G L
Sbjct: 298 YINRDSVIKELLANAVLINEVGKL 321
>sp|P53599|SSK2_YEAST MAP kinase kinase kinase SSK2 (Suppressor of sensor kinase 2)
Length = 1579
Score = 51.6 bits (122), Expect = 3e-06
Identities = 36/159 (22%), Positives = 75/159 (47%)
Frame = +2
Query: 284 ALLELAKTANKNREKTIEIVEKAIENRQIGIEMDLKCSFDCDHEFCLISFMMIEFDKSDV 463
+++EL + +N +T ++++ + + + + + IS + + + K +
Sbjct: 1223 SIMELEERIKRNPHQTGKVIDDSDKGNKYLVSLASS-----------ISNVSMRWQKRNF 1271
Query: 464 ITCGRFAKVFKVVESKENRVFAVKKVTWEQEQADNFPEKENNLHEIKKDAKWLENNINDY 643
I G F +V+ V+ + AVK++ + ++ + IK++ LE
Sbjct: 1272 IGGGTFGRVYSAVDLDNGEILAVKEINIQDSKS-----MQKIFPLIKEEMSVLE------ 1320
Query: 644 KTILSHPHIIKYFGAIKEDYKIFIFMEYAENGSIHNLIQ 760
IL+HP+I+ Y+G K+ IFMEY E GS+ L++
Sbjct: 1321 --ILNHPNIVSYYGVEVHRDKVNIFMEYCEGGSLAALLE 1357
>sp|Q9M8S6|SKOR_ARATH Potassium channel SKOR (Stelar K(+) outward rectifying channel)
Length = 828
Score = 50.8 bits (120), Expect = 5e-06
Identities = 24/75 (32%), Positives = 41/75 (54%), Gaps = 2/75 (2%)
Frame = +2
Query: 74 NKPENEGYYPIHLAGINNAVDVFKYFLQQNCDIELLDSRRNRPIYHAIKNGSEEIVKLLL 253
NK + +G P+HLA D+ Y +Q++ D+ + D + P+ AIKNG++ + LL+
Sbjct: 575 NKTDYDGRSPLHLAASRGYEDITLYLIQESVDVNIKDKLGSTPLLEAIKNGNDRVAALLV 634
Query: 254 --GHRCSFDNGGALL 292
G + +N G L
Sbjct: 635 KEGATLNIENAGTFL 649
>sp|Q9Y6E0|STK24_HUMAN Serine/threonine-protein kinase 24 (STE20-like kinase MST3) (MST-3)
(Mammalian STE20-like protein kinase 3)
Length = 443
Score = 48.9 bits (115), Expect = 2e-05
Identities = 31/108 (28%), Positives = 57/108 (52%), Gaps = 3/108 (2%)
Frame = +2
Query: 446 FDKSDVITCGRFAKVFKVVESKENRVFAVKKVTWEQEQADNFPEKENNLHEIKKDAKWLE 625
F K + I G F +VFK ++++ +V A+K + E E E+ + +I+++
Sbjct: 36 FTKLEKIGKGSFGEVFKGIDNRTQKVVAIKIIDLE--------EAEDEIEDIQQEI---- 83
Query: 626 NNINDYKTILSH---PHIIKYFGAIKEDYKIFIFMEYAENGSIHNLIQ 760
T+LS P++ KY+G+ +D K++I MEY GS +L++
Sbjct: 84 -------TVLSQCDSPYVTKYYGSYLKDTKLWIIMEYLGGGSALDLLE 124
>sp|P47810|WEE1_MOUSE Wee1-like protein kinase
Length = 646
Score = 48.9 bits (115), Expect = 2e-05
Identities = 30/111 (27%), Positives = 53/111 (47%)
Frame = +2
Query: 443 EFDKSDVITCGRFAKVFKVVESKENRVFAVKKVTWEQEQADNFPEKENNLHEIKKDAKWL 622
EF + + I G F VFK V+ + ++A+K+ ++ +++N L E+ A
Sbjct: 297 EFHELEKIGSGEFGSVFKCVKRLDGCIYAIKR---SKKPLAGSVDEQNALREVYAHA--- 350
Query: 623 ENNINDYKTILSHPHIIKYFGAIKEDYKIFIFMEYAENGSIHNLIQKAIKV 775
+ HPH+++YF A ED + I EY GS+ + I + +V
Sbjct: 351 --------VLGQHPHVVRYFSAWAEDDHMLIQNEYCNGGSLADAISENYRV 393
>sp|Q99KH8|STK24_MOUSE Serine/threonine-protein kinase 24
Length = 431
Score = 48.9 bits (115), Expect = 2e-05
Identities = 31/108 (28%), Positives = 57/108 (52%), Gaps = 3/108 (2%)
Frame = +2
Query: 446 FDKSDVITCGRFAKVFKVVESKENRVFAVKKVTWEQEQADNFPEKENNLHEIKKDAKWLE 625
F K + I G F +VFK ++++ +V A+K + E E E+ + +I+++
Sbjct: 24 FTKLEKIGKGSFGEVFKGIDNRTQKVVAIKIIDLE--------EAEDEIEDIQQEI---- 71
Query: 626 NNINDYKTILSH---PHIIKYFGAIKEDYKIFIFMEYAENGSIHNLIQ 760
T+LS P++ KY+G+ +D K++I MEY GS +L++
Sbjct: 72 -------TVLSQCDSPYVTKYYGSYLKDTKLWIIMEYLGGGSALDLLE 112
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 96,742,069
Number of Sequences: 369166
Number of extensions: 1929167
Number of successful extensions: 8023
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 6803
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 7925
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 8646143400
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)