Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_006_O16
(802 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q01241|NF70_LOLPE 70 kDa neurofilament protein (NF70) 130 4e-30
sp|Q01240|NF60_LOLPE 60 kDa neurofilament protein (NF60) 130 4e-30
sp|P90901|IFA1_CAEEL Intermediate filament protein ifa-1 (I... 110 3e-24
sp|P22488|IFEA_HELAS Non-neuronal cytoplasmic intermediate ... 107 4e-23
sp|P90900|IFA4_CAEEL Intermediate filament protein ifa-4 (I... 106 8e-23
sp|P16275|IFEB_HELPO Non-neuronal cytoplasmic intermediate ... 105 1e-22
sp|O02365|IFA2_CAEEL Intermediate filament protein ifa-2 (I... 104 3e-22
sp|P24789|VIM1_XENLA Vimentins 1 and 2 101 3e-21
sp|P23730|IFEA_ASCSU Intermediate filament protein A (IF-A) 99 2e-20
sp|Q21065|IFA3_CAEEL Intermediate filament protein ifa-3 (I... 98 3e-20
>sp|Q01241|NF70_LOLPE 70 kDa neurofilament protein (NF70)
Length = 615
Score = 130 bits (327), Expect = 4e-30
Identities = 67/173 (38%), Positives = 108/173 (62%)
Frame = +2
Query: 278 SNVNSMRKGRAQEKRDMQDLNERFASYIEKARFLEAQNKQLVGEIEQLKGGSVKDTSKIK 457
+ VN ++ R +EK+DM+DLNERFA+YIEK RFLEAQNK+L GE+E+LK K+TS IK
Sbjct: 84 TGVNHVKANREREKQDMRDLNERFANYIEKVRFLEAQNKKLAGELEELKSKWGKETSAIK 143
Query: 458 EIYEVEIRQLRNLLNDSEKNRAPLEVQIDSLEYELDDLRKRLMLLEDINGQYKSTIDKQR 637
E+YE E+ + R L++ + K + L+V++ L +L+ +K L + + I +Q
Sbjct: 144 EMYETELEEARKLIDATNKEKITLDVRVTELIDQLERQQKDLEESRTYHQIDQEQIARQN 203
Query: 638 QELGNFEGELVELRRFKNTRNADVSKEKEEVKSLRDELKKLRQDYEREALAHI 796
Q+L + EGE+ LRR + + ++ + + DE++K+R D E + H+
Sbjct: 204 QQLADLEGEISMLRRSIESLEKEKMRQSNILAKMNDEMEKMRMDLNNETINHL 256
>sp|Q01240|NF60_LOLPE 60 kDa neurofilament protein (NF60)
Length = 511
Score = 130 bits (327), Expect = 4e-30
Identities = 67/173 (38%), Positives = 108/173 (62%)
Frame = +2
Query: 278 SNVNSMRKGRAQEKRDMQDLNERFASYIEKARFLEAQNKQLVGEIEQLKGGSVKDTSKIK 457
+ VN ++ R +EK+DM+DLNERFA+YIEK RFLEAQNK+L GE+E+LK K+TS IK
Sbjct: 84 TGVNHVKANREREKQDMRDLNERFANYIEKVRFLEAQNKKLAGELEELKSKWGKETSAIK 143
Query: 458 EIYEVEIRQLRNLLNDSEKNRAPLEVQIDSLEYELDDLRKRLMLLEDINGQYKSTIDKQR 637
E+YE E+ + R L++ + K + L+V++ L +L+ +K L + + I +Q
Sbjct: 144 EMYETELEEARKLIDATNKEKITLDVRVTELIDQLERQQKDLEESRTYHQIDQEQIARQN 203
Query: 638 QELGNFEGELVELRRFKNTRNADVSKEKEEVKSLRDELKKLRQDYEREALAHI 796
Q+L + EGE+ LRR + + ++ + + DE++K+R D E + H+
Sbjct: 204 QQLADLEGEISMLRRSIESLEKEKMRQSNILAKMNDEMEKMRMDLNNETINHL 256
>sp|P90901|IFA1_CAEEL Intermediate filament protein ifa-1 (Intermediate filament protein
A1) (IF-A1) (Cel IF A1)
Length = 575
Score = 110 bits (276), Expect = 3e-24
Identities = 61/174 (35%), Positives = 103/174 (59%)
Frame = +2
Query: 275 QSNVNSMRKGRAQEKRDMQDLNERFASYIEKARFLEAQNKQLVGEIEQLKGGSVKDTSKI 454
Q+ +++R R +EK++M DLN+R ASYIEK RFLEAQN++L +++ L+ KDT I
Sbjct: 56 QNAASTIRDSREREKKEMSDLNDRLASYIEKVRFLEAQNRKLAADLDALRSKWGKDTHNI 115
Query: 455 KEIYEVEIRQLRNLLNDSEKNRAPLEVQIDSLEYELDDLRKRLMLLEDINGQYKSTIDKQ 634
+ +YE E+ + L++++ K R +E Q+ ++ EL ++R++L Q ++ ID
Sbjct: 116 RNMYEGELVDAQKLIDETNKQRKDMEGQLKKMQDELAEMRRKLEDATKGREQDRAKIDAL 175
Query: 635 RQELGNFEGELVELRRFKNTRNADVSKEKEEVKSLRDELKKLRQDYEREALAHI 796
L N E E+ L+R +V + K+E + L EL++ R D ++E L I
Sbjct: 176 LVTLSNLEAEISLLKRRIAQLEDEVKRIKQENQRLLSELQRARTDLDQETLNRI 229
>sp|P22488|IFEA_HELAS Non-neuronal cytoplasmic intermediate filament protein (IF)
Length = 576
Score = 107 bits (267), Expect = 4e-23
Identities = 57/173 (32%), Positives = 99/173 (57%)
Frame = +2
Query: 278 SNVNSMRKGRAQEKRDMQDLNERFASYIEKARFLEAQNKQLVGEIEQLKGGSVKDTSKIK 457
S + R R +EKR+MQ+LNER ASYIEK FL+AQ K+L E E L+ + I+
Sbjct: 61 SGITDFRGTREKEKREMQNLNERLASYIEKVHFLDAQVKKLEAENEALRNRKSESLQPIR 120
Query: 458 EIYEVEIRQLRNLLNDSEKNRAPLEVQIDSLEYELDDLRKRLMLLEDINGQYKSTIDKQR 637
+ YE E+ Q R ++++ + E ++ L+ E+ LR+ ++ E+ + Y+ I+
Sbjct: 121 DAYENELAQARKVIDELSSTKGVSEAKVAGLQDEIASLRELIVTYENQSKDYRKKIESLG 180
Query: 638 QELGNFEGELVELRRFKNTRNADVSKEKEEVKSLRDELKKLRQDYEREALAHI 796
++G +EGEL LR + + +K +E + ++++ ++LR D + E AHI
Sbjct: 181 NQIGEYEGELHTLRIRCGSLEDENAKVRELLDKIQEQNRRLRADLDTETAAHI 233
>sp|P90900|IFA4_CAEEL Intermediate filament protein ifa-4 (Intermediate filament protein
A4) (IF-A4) (Cel IF A4)
Length = 575
Score = 106 bits (264), Expect = 8e-23
Identities = 61/170 (35%), Positives = 96/170 (56%)
Frame = +2
Query: 287 NSMRKGRAQEKRDMQDLNERFASYIEKARFLEAQNKQLVGEIEQLKGGSVKDTSKIKEIY 466
+++R+ R +EK++M +LN+R ASYIEK RFLEAQN+++ ++ L+G D++ +K +Y
Sbjct: 61 SAIRESRTREKKEMSELNDRLASYIEKVRFLEAQNRKMEKDLNLLRGKWGHDSTSVKVMY 120
Query: 467 EVEIRQLRNLLNDSEKNRAPLEVQIDSLEYELDDLRKRLMLLEDINGQYKSTIDKQRQEL 646
E E+R ++L+ DS+K RA LE QI L EL++ R +L E + +D L
Sbjct: 121 ETELRSAKDLIADSDKERAQLEDQIKKLVEELNNYRNKLHEAERGADVTRKELDNVLGRL 180
Query: 647 GNFEGELVELRRFKNTRNADVSKEKEEVKSLRDELKKLRQDYEREALAHI 796
G E E+ L+R + + K E + D L R E+E L I
Sbjct: 181 GALEAEIELLKRRISLMEESIGHLKRENHRMIDSLHHARNAVEQETLNRI 230
>sp|P16275|IFEB_HELPO Non-neuronal cytoplasmic intermediate filament protein B (IFB)
Length = 450
Score = 105 bits (262), Expect = 1e-22
Identities = 57/173 (32%), Positives = 97/173 (56%)
Frame = +2
Query: 278 SNVNSMRKGRAQEKRDMQDLNERFASYIEKARFLEAQNKQLVGEIEQLKGGSVKDTSKIK 457
S + R R +EKR+MQ+LNER A YIEK FL+AQ K+L E E L+ + I+
Sbjct: 59 SGITDFRGTREKEKREMQNLNERLAGYIEKVHFLDAQVKKLEAENEALRNRKSESLQPIR 118
Query: 458 EIYEVEIRQLRNLLNDSEKNRAPLEVQIDSLEYELDDLRKRLMLLEDINGQYKSTIDKQR 637
+ YE E+ Q R ++++ + E ++ L+ E+ LR+ + E+ + Y+ I+
Sbjct: 119 DAYENELAQARKVIDELSSTKGVSEAKVAGLQDEIASLRELIGTYENQSKDYRKKIESLG 178
Query: 638 QELGNFEGELVELRRFKNTRNADVSKEKEEVKSLRDELKKLRQDYEREALAHI 796
++G +EGEL LR + + +K +E + ++D+ ++LR D + E AHI
Sbjct: 179 NQIGEYEGELHTLRIRCGSLEDENAKVRELLDKIQDQNRRLRADLDTETSAHI 231
>sp|O02365|IFA2_CAEEL Intermediate filament protein ifa-2 (Intermediate filament protein
A2) (IF-A2) (Cel IF A2)
Length = 581
Score = 104 bits (259), Expect = 3e-22
Identities = 58/174 (33%), Positives = 102/174 (58%)
Frame = +2
Query: 275 QSNVNSMRKGRAQEKRDMQDLNERFASYIEKARFLEAQNKQLVGEIEQLKGGSVKDTSKI 454
Q+ +++R R +EK+++ +LN+R ASYIEK RFL+AQN++L +++ L+G K T +
Sbjct: 58 QNAASTIRDNREREKKEIMELNDRLASYIEKVRFLDAQNRKLDADLKMLQGRFGKSTGSV 117
Query: 455 KEIYEVEIRQLRNLLNDSEKNRAPLEVQIDSLEYELDDLRKRLMLLEDINGQYKSTIDKQ 634
K +YE+EI N++ + K+ E +I L+ +LD+LRK+ + + + ID
Sbjct: 118 KVMYEMEITTATNVVKQTGKDHGETEKEIRKLQDQLDELRKKFEEAQRGRAEDRLKIDDL 177
Query: 635 RQELGNFEGELVELRRFKNTRNADVSKEKEEVKSLRDELKKLRQDYEREALAHI 796
L N E E+ L+R +V++ K+E L EL+++R + ++E L I
Sbjct: 178 LVTLSNLEAEINLLKRRIALLEEEVARLKKENFRLTSELQRVRIELDQETLLRI 231
Score = 48.1 bits (113), Expect = 3e-05
Identities = 41/187 (21%), Positives = 89/187 (47%), Gaps = 22/187 (11%)
Frame = +2
Query: 272 LQSNVNSMRKGRAQEKRDMQDLNERFASYIEKARFLEAQNKQLVGEIEQLKGGSVKDTSK 451
L+ ++GRA+++ + DL ++ + L+ + L E+ +LK + + TS+
Sbjct: 156 LRKKFEEAQRGRAEDRLKIDDLLVTLSNLEAEINLLKRRIALLEEEVARLKKENFRLTSE 215
Query: 452 IKEIYEVEIRQLRNLLNDSEKNRAPLEVQIDSL----EYELDDLRKRLM----------- 586
++ + +E+ Q L D++ + +ID + E EL +L+ +
Sbjct: 216 LQRV-RIELDQETLLRIDNQNKVKTILEEIDFMKRGFETELKELQAQAARDTTSENREYF 274
Query: 587 ------LLEDINGQYKSTIDKQRQELGN-FEGELVELRRFKNTRNADVSKEKEEVKSLRD 745
+ DI +Y ++ R ++ + ++ + E+ N +NA+ + +KEEVK LR+
Sbjct: 275 KNELANAMRDIRAEYDQIMNGNRNDMESWYQLRVQEINTQSNRQNAENNYQKEEVKRLRN 334
Query: 746 ELKKLRQ 766
+ +LRQ
Sbjct: 335 QTSELRQ 341
Score = 32.0 bits (71), Expect = 2.0
Identities = 48/193 (24%), Positives = 81/193 (41%), Gaps = 29/193 (15%)
Frame = +2
Query: 284 VNSMRKGRAQEKRDMQDLNERFASYIEKARF---LEAQNKQLVGEIEQLKGGSVKDTS-- 448
++ M++G E +++Q R + + F L + + E +Q+ G+ D
Sbjct: 244 IDFMKRGFETELKELQAQAARDTTSENREYFKNELANAMRDIRAEYDQIMNGNRNDMESW 303
Query: 449 ---KIKEI-------------YEVEIRQLRN-------LLNDSEKNRAPLEVQIDSLEYE 559
+++EI + E+++LRN L+D E LE QI+ L Y+
Sbjct: 304 YQLRVQEINTQSNRQNAENNYQKEEVKRLRNQTSELRQKLSDLESRNLLLEKQIEDLNYQ 363
Query: 560 L-DDLRKRLMLLEDINGQYKSTIDKQRQELGNFEGELVELRRFKNTRNADVSKEKEEVKS 736
L DD R L D + Q I K R+E + +VEL+ +T+ ++
Sbjct: 364 LEDDQRSYEAALNDKDAQ----IRKLREEC---QALMVELQMLLDTK-----------QT 405
Query: 737 LRDELKKLRQDYE 775
L ELK RQ E
Sbjct: 406 LDGELKVYRQMLE 418
>sp|P24789|VIM1_XENLA Vimentins 1 and 2
Length = 458
Score = 101 bits (251), Expect = 3e-21
Identities = 74/223 (33%), Positives = 117/223 (52%), Gaps = 3/223 (1%)
Frame = +2
Query: 122 TTSRSYYKPISQPRNNLRDLQKTAGISLNAQMSDQQGGSMVPLIRGDSVALQSNVNSMRK 301
T+SR Y S P + K++ + L + + + V D+V L+ N
Sbjct: 42 TSSRMVYSTSSSPA-----VFKSSSVRLRSSLPPARMADSVDFALADAVNLEFKAN---- 92
Query: 302 GRAQEKRDMQDLNERFASYIEKARFLEAQNKQLVGEIEQLKGGSVKDTSKIKEIYEVEIR 481
R EK +M +LN+RFA++I+K RFLE QNK LV E+EQLKG K TS+I ++YE E+R
Sbjct: 93 -RTNEKAEMIELNDRFANFIDKVRFLEQQNKILVAELEQLKG---KGTSRIGDLYEEEMR 148
Query: 482 QLRNLLNDSEKNRAPLEVQIDSLEYELDDLRKRLMLLEDINGQYKSTIDKQRQELGNFEG 661
+LR L+ + ++A +EV D+L +L LR++L + + + RQ++ N
Sbjct: 149 ELRRQLDQATNDKARVEVDRDNLADDLQRLREKLQDEMIQKEEAEGNLQSFRQDVDNASL 208
Query: 662 ELVELRRFKNTRNADVSKEKEEVKSLRDE---LKKLRQDYERE 781
++L R +V+SL++E LKKL + RE
Sbjct: 209 ARIDLER--------------KVESLQEEIAFLKKLHDEEIRE 237
>sp|P23730|IFEA_ASCSU Intermediate filament protein A (IF-A)
Length = 497
Score = 98.6 bits (244), Expect = 2e-20
Identities = 58/159 (36%), Positives = 92/159 (57%), Gaps = 2/159 (1%)
Frame = +2
Query: 326 MQDLNERFASYIEKARFLEAQNKQLVGEIEQLKGGSVKDTSKIKEIYEVEIRQLRNLLND 505
M DLN+R ASYIEK RFLEAQN++L +++ L+G KDT ++ +YE E+++ R L+ND
Sbjct: 1 MSDLNDRLASYIEKVRFLEAQNRKLAADLDLLRGRWGKDTLSVRAMYEGELQEARKLVND 60
Query: 506 SEKNRAPLEVQIDSLEYELDDLRKRLMLLEDINGQY--KSTIDKQRQELGNFEGELVELR 679
+ + R LE +I L EL + R++ + + G + ID+ +L E E+ LR
Sbjct: 61 TNRQREELEKEIRKLLDELSEYRRKYE--DALRGHQIDRDRIDELLNQLSGLEAEINLLR 118
Query: 680 RFKNTRNADVSKEKEEVKSLRDELKKLRQDYEREALAHI 796
R ++++ K+E +EL K R D ++E L I
Sbjct: 119 RRIANIIEEIARIKKENLQFANELTKARSDLDQETLNRI 157
>sp|Q21065|IFA3_CAEEL Intermediate filament protein ifa-3 (Intermediate filament protein
A3) (IF-A3) (Cel IF A3)
Length = 581
Score = 97.8 bits (242), Expect = 3e-20
Identities = 55/174 (31%), Positives = 99/174 (56%)
Frame = +2
Query: 275 QSNVNSMRKGRAQEKRDMQDLNERFASYIEKARFLEAQNKQLVGEIEQLKGGSVKDTSKI 454
Q +++R R +EK+++ +LN+R ASYI K RFL AQN++L ++ L+ K T +
Sbjct: 58 QGAASTIRDDREREKKEITELNDRLASYIGKVRFLAAQNRKLEADLNVLQSRFGKSTGSV 117
Query: 455 KEIYEVEIRQLRNLLNDSEKNRAPLEVQIDSLEYELDDLRKRLMLLEDINGQYKSTIDKQ 634
K +YE+EI N++ ++ K+ E +I ++ +LD+LRK+ + + + ID+
Sbjct: 118 KIMYEMEITTATNVVKETGKDHEEAEKEIGKIKDQLDELRKKFEEAQKGRAEDRLKIDEL 177
Query: 635 RQELGNFEGELVELRRFKNTRNADVSKEKEEVKSLRDELKKLRQDYEREALAHI 796
L N E E+ L+R +V++ K+E L EL+++R + ++E L I
Sbjct: 178 LVTLSNLEAEINLLKRRIALLEEEVARLKKENFRLTSELQRVRSELDQETLLRI 231
Score = 41.6 bits (96), Expect = 0.003
Identities = 39/189 (20%), Positives = 87/189 (46%), Gaps = 22/189 (11%)
Frame = +2
Query: 272 LQSNVNSMRKGRAQEKRDMQDLNERFASYIEKARFLEAQNKQLVGEIEQLKGGSVKDTSK 451
L+ +KGRA+++ + +L ++ + L+ + L E+ +LK + + TS+
Sbjct: 156 LRKKFEEAQKGRAEDRLKIDELLVTLSNLEAEINLLKRRIALLEEEVARLKKENFRLTSE 215
Query: 452 IKEIYEVEIRQLRNLLNDSEKNRAPLEVQIDSL----EYELDDLRKR------------- 580
++ + E+ Q L D++ + +ID + E EL DL+ +
Sbjct: 216 LQRVRS-ELDQETLLRIDNQNKVTTILEEIDFMKRGFETELKDLQAQAARDTTSENREYF 274
Query: 581 ----LMLLEDINGQYKSTIDKQRQELGNFEGELV-ELRRFKNTRNADVSKEKEEVKSLRD 745
+ + DI +Y + R +L ++ V E+ N +NA+++ +++EVK L
Sbjct: 275 KNELMNSIRDIRAEYDRFMAGNRNDLESWSQIRVQEINTQTNRQNAEINHKRDEVKRLHS 334
Query: 746 ELKKLRQDY 772
++ +L+ +
Sbjct: 335 QVSELKSKH 343
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 66,041,649
Number of Sequences: 369166
Number of extensions: 1062355
Number of successful extensions: 6399
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5262
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6109
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 7570361805
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)