Planarian EST Database


Dr_sW_006_M17

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_006_M17
         (910 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P13906|MTB1_BACSH  Modification methylase BspRI (Cytosine...    37   0.076
sp|Q86UE8|TLK2_HUMAN  Serine/threonine-protein kinase tousle...    37   0.100
sp|O55047|TLK2_MOUSE  Serine/threonine-protein kinase tousle...    37   0.100
sp|O15205|FAT10_HUMAN  Ubiquitin-like protein FAT10 (Diubiqu...    33   0.84 
sp|Q8ICR0|CDPK2_PLAF7  Calcium-dependent protein kinase 2 (P...    33   1.1  
sp|O15865|CDPK2_PLAFK  Calcium-dependent protein kinase 2 (P...    33   1.1  
sp|P06530|MTBR_BACSU  Modification methylase BsuRI (Cytosine...    33   1.4  
sp|P47460|HMW2_MYCGE  Cytadherence high molecular weight pro...    33   1.4  
sp|Q8K1Y2|KPCN_MOUSE  Protein kinase C, nu type (nPKC-nu)          32   1.9  
sp|O94806|KPCN_HUMAN  Protein kinase C, nu type (nPKC-nu) (P...    32   1.9  
>sp|P13906|MTB1_BACSH Modification methylase BspRI (Cytosine-specific methyltransferase
           BspRI) (M.BspRI)
          Length = 424

 Score = 37.0 bits (84), Expect = 0.076
 Identities = 19/59 (32%), Positives = 32/59 (54%)
 Frame = +2

Query: 506 DYILLFNEKKHQRNPFIYPYINLEDSFIKLMKIPDVKIDPEPFDTGMYSEIYKARDQVK 682
           D+   + E  H     + PY+ LE++      I D+ +DP P+ TG YS I+ +R++ K
Sbjct: 250 DFNYEYPEITHGNEEGLKPYVTLEEA------IGDLSLDPGPYFTGSYSTIFMSRNRKK 302
>sp|Q86UE8|TLK2_HUMAN Serine/threonine-protein kinase tousled-like 2 (Tousled-like kinase
           2) (PKU-alpha)
          Length = 772

 Score = 36.6 bits (83), Expect = 0.100
 Identities = 25/87 (28%), Positives = 42/87 (48%), Gaps = 4/87 (4%)
 Frame = +2

Query: 641 GMYSEIYKARDQVKNQSIVLKRYISDDG---DLIEKYRKLINGLIDFQRKGGQERFVKLY 811
           G +SE+YKA D  + + + +K +  +     +  E Y K         ++    R VKLY
Sbjct: 471 GGFSEVYKAFDLTEQRYVAVKIHQLNKNWRDEKKENYHKHACREYRIHKELDHPRIVKLY 530

Query: 812 SFYP-DPCSFAIGMEWMEGGSMDNYVK 889
            ++  D  SF   +E+ EG  +D Y+K
Sbjct: 531 DYFSLDTDSFCTVLEYCEGNDLDFYLK 557
>sp|O55047|TLK2_MOUSE Serine/threonine-protein kinase tousled-like 2 (Tousled-like kinase
           2)
          Length = 718

 Score = 36.6 bits (83), Expect = 0.100
 Identities = 25/87 (28%), Positives = 42/87 (48%), Gaps = 4/87 (4%)
 Frame = +2

Query: 641 GMYSEIYKARDQVKNQSIVLKRYISDDG---DLIEKYRKLINGLIDFQRKGGQERFVKLY 811
           G +SE+YKA D  + + + +K +  +     +  E Y K         ++    R VKLY
Sbjct: 417 GGFSEVYKAFDLTEQRYVAVKIHQLNKNWRDEKKENYHKHACREYRIHKELDHPRIVKLY 476

Query: 812 SFYP-DPCSFAIGMEWMEGGSMDNYVK 889
            ++  D  SF   +E+ EG  +D Y+K
Sbjct: 477 DYFSLDTDSFCTVLEYCEGNDLDFYLK 503
>sp|O15205|FAT10_HUMAN Ubiquitin-like protein FAT10 (Diubiquitin)
          Length = 165

 Score = 33.5 bits (75), Expect = 0.84
 Identities = 24/93 (25%), Positives = 45/93 (48%), Gaps = 2/93 (2%)
 Frame = +2

Query: 2   KIKQKIRDNLSCPKNVNIKLMKLKYNDTFLNDNELLLS--TVKEEGATVTFELYSENDAK 175
           KIK+ +R     P    + L+  K     L     L S    KE+   +T ++   +D +
Sbjct: 32  KIKEHVRSKTKVPVQDQVLLLGSK----ILKPRRSLSSYGIDKEKTIHLTLKVVKPSDEE 87

Query: 176 NDVILLFQGDMPARQILQLPKTATIREVKAALE 274
             + L+  GD   R +LQ+ +++++ +VKA +E
Sbjct: 88  LPLFLVESGDEAKRHLLQVRRSSSVAQVKAMIE 120
>sp|Q8ICR0|CDPK2_PLAF7 Calcium-dependent protein kinase 2 (PfCDPK2)
          Length = 509

 Score = 33.1 bits (74), Expect = 1.1
 Identities = 27/99 (27%), Positives = 42/99 (42%), Gaps = 1/99 (1%)
 Frame = +2

Query: 599 KIPDVKIDPEPFDTGMYSEIYKARDQVKNQSIVLKRYISDDGDLIEKYRKLINGLIDFQR 778
           K+ D  I  E    G Y  +YK  D+V NQ   +K    D    I ++ +     I+  +
Sbjct: 67  KLEDKYIIDEKLGQGTYGCVYKGIDKVTNQLYAIKEEKKDRLKNINRFFQ----EIEIMK 122

Query: 779 KGGQERFVKLYSFYPDPCSFAIGMEWMEGGSM-DNYVKN 892
           K      VKLY  Y +     + ME   G  + D+ ++N
Sbjct: 123 KLDHPNIVKLYETYENDNYIYLIMELCSGRELFDSIIEN 161
>sp|O15865|CDPK2_PLAFK Calcium-dependent protein kinase 2 (PfCDPK2)
          Length = 513

 Score = 33.1 bits (74), Expect = 1.1
 Identities = 27/99 (27%), Positives = 42/99 (42%), Gaps = 1/99 (1%)
 Frame = +2

Query: 599 KIPDVKIDPEPFDTGMYSEIYKARDQVKNQSIVLKRYISDDGDLIEKYRKLINGLIDFQR 778
           K+ D  I  E    G Y  +YK  D+V NQ   +K    D    I ++ +     I+  +
Sbjct: 67  KLEDKYIIDEKLGQGTYGCVYKGIDKVTNQLYAIKEEKKDRLKNINRFFQ----EIEIMK 122

Query: 779 KGGQERFVKLYSFYPDPCSFAIGMEWMEGGSM-DNYVKN 892
           K      VKLY  Y +     + ME   G  + D+ ++N
Sbjct: 123 KLDHPNIVKLYETYENDNYIYLIMELCSGRELFDSIIEN 161
>sp|P06530|MTBR_BACSU Modification methylase BsuRI (Cytosine-specific methyltransferase
           BsuRI) (M.BsuRI)
          Length = 436

 Score = 32.7 bits (73), Expect = 1.4
 Identities = 18/50 (36%), Positives = 26/50 (52%)
 Frame = +2

Query: 536 HQRNPFIYPYINLEDSFIKLMKIPDVKIDPEPFDTGMYSEIYKARDQVKN 685
           H     + P+  L DS      I D+  DP P+ TG YS IY +R++ K+
Sbjct: 261 HGEETGLKPFKTLRDS------IGDLVTDPGPYFTGSYSSIYMSRNRKKS 304
>sp|P47460|HMW2_MYCGE Cytadherence high molecular weight protein 2 (Cytadherence
           accessory protein 2)
          Length = 1805

 Score = 32.7 bits (73), Expect = 1.4
 Identities = 20/94 (21%), Positives = 45/94 (47%), Gaps = 8/94 (8%)
 Frame = +2

Query: 524 NEKKHQRNPFIYPYINLEDSFIKLMKIPDVKIDPEPFDTGMYSEIYKARDQVKNQSIVL- 700
           N+  ++ N  I    NL D+ + L++ P++ ++ + +   +  ++Y   DQ++NQ  +L 
Sbjct: 156 NQTINRLNQKINGNQNLIDNNVALLQNPNITVEKKNYLLNVIDQLYNELDQLENQKRLLS 215

Query: 701 -------KRYISDDGDLIEKYRKLINGLIDFQRK 781
                  +  +S D +L   Y  +    I F+ +
Sbjct: 216 IEYENTYRELVSADNELQNVYENIDQNQIQFKHQ 249
>sp|Q8K1Y2|KPCN_MOUSE Protein kinase C, nu type (nPKC-nu)
          Length = 889

 Score = 32.3 bits (72), Expect = 1.9
 Identities = 30/87 (34%), Positives = 43/87 (49%), Gaps = 11/87 (12%)
 Frame = +2

Query: 101 ELLLSTVKEEGATVTFELYSEND--AKNDVILLFQGDMPARQILQLPKTA-TIRE---VK 262
           EL LS VK+   ++ ++ + E       D ILLF+ DM +  ILQL  +A  I E   V+
Sbjct: 79  ELSLSAVKDLVCSIVYQKFPECGFFGMYDKILLFRHDMNSENILQLITSADEIHEGDLVE 138

Query: 263 AALEKLQEIETFD-----LYNYSIELP 328
             L  L  +E F      LY +S + P
Sbjct: 139 VVLSALATVEDFQIRPHALYVHSYKAP 165
>sp|O94806|KPCN_HUMAN Protein kinase C, nu type (nPKC-nu) (Protein kinase EPK2)
          Length = 890

 Score = 32.3 bits (72), Expect = 1.9
 Identities = 30/87 (34%), Positives = 43/87 (49%), Gaps = 11/87 (12%)
 Frame = +2

Query: 101 ELLLSTVKEEGATVTFELYSEND--AKNDVILLFQGDMPARQILQLPKTA-TIRE---VK 262
           EL LS VK+   ++ ++ + E       D ILLF+ DM +  ILQL  +A  I E   V+
Sbjct: 79  ELSLSAVKDLVCSIVYQKFPECGFFGMYDKILLFRHDMNSENILQLITSADEIHEGDLVE 138

Query: 263 AALEKLQEIETFD-----LYNYSIELP 328
             L  L  +E F      LY +S + P
Sbjct: 139 VVLSALATVEDFQIRPHTLYVHSYKAP 165
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 99,823,682
Number of Sequences: 369166
Number of extensions: 1964691
Number of successful extensions: 5729
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5510
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5724
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 9222552960
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)