Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_006_L10
(657 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9N4M4|ANC1_CAEEL Nuclear anchorage protein 1 (Anchorage... 36 0.077
sp|P25980|UBF1B_XENLA Nucleolar transcription factor 1-B (U... 35 0.17
sp|P32773|TOA1_YEAST Transcription initiation factor IIA la... 34 0.38
sp|P36044|MNN4_YEAST Protein MNN4 34 0.38
sp|P24788|CD2L1_MOUSE PITSLRE serine/threonine-protein kina... 33 0.50
sp|P46916|YTXO_BACSU Hypothetical protein ytxO (ORFY) 33 0.50
sp|Q6FSW2|RAD52_CANGA DNA repair and recombination protein ... 33 0.65
sp|Q6DRL5|MBB1A_BRARE Myb-binding protein 1A-like protein 33 0.85
sp|P13469|MODU_DROME DNA-binding protein modulo 32 1.1
sp|Q960E8|TF2H1_DROME TFIIH basal transcription factor comp... 32 1.1
>sp|Q9N4M4|ANC1_CAEEL Nuclear anchorage protein 1 (Anchorage 1 protein) (Nesprin homolog)
Length = 8545
Score = 36.2 bits (82), Expect = 0.077
Identities = 22/64 (34%), Positives = 35/64 (54%), Gaps = 2/64 (3%)
Frame = +2
Query: 104 TVENITPVTEEKNNLKEVIEKKTVDSENVDNDEPETKK--QRLSDLNDIKDSEEKAQNGD 277
T+E +TP+ EE L + IEKK + V ++P + Q+L D+ I + EE N D
Sbjct: 2046 TIETVTPILEEYTTLVDNIEKKLANEIEVPTNDPSRQDLVQQLQDV--ILECEEVVVNCD 2103
Query: 278 DAEE 289
+ E+
Sbjct: 2104 NIEK 2107
>sp|P25980|UBF1B_XENLA Nucleolar transcription factor 1-B (Upstream binding factor 1-B)
(UBF-1-B) (xUBF-2)
Length = 701
Score = 35.0 bits (79), Expect = 0.17
Identities = 24/114 (21%), Positives = 52/114 (45%)
Frame = +2
Query: 110 ENITPVTEEKNNLKEVIEKKTVDSENVDNDEPETKKQRLSDLNDIKDSEEKAQNGDDAEE 289
++ T + + K VI+ K+ D E+ ++DE E D +D +D E+ +++GD ++
Sbjct: 594 KSTTKIQAPSSKSKLVIQSKSDDDEDDEDDEDEED----DDDDDDEDKEDSSEDGDSSDS 649
Query: 290 DQXXXXXXXXXXXXXXXXXXXSEKGTEEHVKEKDCNGSSAKVTENGDSAENEVN 451
E E+ + + +GSS+ + + DS++++ N
Sbjct: 650 SSDEDSEEGEENEDEEDEEDDDEDNEED--DDDNESGSSSSSSSSADSSDSDSN 701
>sp|P32773|TOA1_YEAST Transcription initiation factor IIA large chain (TFIIA 32 kDa
subunit)
Length = 286
Score = 33.9 bits (76), Expect = 0.38
Identities = 20/68 (29%), Positives = 41/68 (60%), Gaps = 2/68 (2%)
Frame = +2
Query: 95 DMSTVENITPVTEEKNN--LKEVIEKKTVDSENVDNDEPETKKQRLSDLNDIKDSEEKAQ 268
+++TVENI +E+K++ +E +EK + E ++ + + KK++ S L D++E
Sbjct: 164 NITTVENIDDESEKKDDEEKEEDVEKTRKEKEQIEQVKLQAKKEKRSAL---LDTDEVGS 220
Query: 269 NGDDAEED 292
DD+++D
Sbjct: 221 ELDDSDDD 228
>sp|P36044|MNN4_YEAST Protein MNN4
Length = 1178
Score = 33.9 bits (76), Expect = 0.38
Identities = 24/105 (22%), Positives = 40/105 (38%)
Frame = +2
Query: 131 EEKNNLKEVIEKKTVDSENVDNDEPETKKQRLSDLNDIKDSEEKAQNGDDAEEDQXXXXX 310
EE+ KE EKK + E E E KK++ + K+ EEK + ++ ++ +
Sbjct: 1047 EEEKKKKEEEEKKKKEEEEKKKKEEEEKKKKEEEEKKKKEEEEKKKQEEEEKKKKEEEEK 1106
Query: 311 XXXXXXXXXXXXXXSEKGTEEHVKEKDCNGSSAKVTENGDSAENE 445
K E+ K+K+ K E E+E
Sbjct: 1107 KKQEEGEKMKNEDEENKKNEDEEKKKNEEEEKKKQEEKNKKNEDE 1151
Score = 30.0 bits (66), Expect = 5.5
Identities = 25/99 (25%), Positives = 41/99 (41%)
Frame = +2
Query: 149 KEVIEKKTVDSENVDNDEPETKKQRLSDLNDIKDSEEKAQNGDDAEEDQXXXXXXXXXXX 328
KE EKK + E E E KK++ + K+ EEK + ++ ++ Q
Sbjct: 1045 KEEEEKKKKEEEEKKKKEEEEKKKKEEEEKKKKEEEEKKKKEEEEKKKQ----------- 1093
Query: 329 XXXXXXXXSEKGTEEHVKEKDCNGSSAKVTENGDSAENE 445
+K EE K+K G K E+ ++ +NE
Sbjct: 1094 -----EEEEKKKKEEEEKKKQEEGEKMK-NEDEENKKNE 1126
Score = 29.3 bits (64), Expect = 9.4
Identities = 16/46 (34%), Positives = 26/46 (56%)
Frame = +2
Query: 131 EEKNNLKEVIEKKTVDSENVDNDEPETKKQRLSDLNDIKDSEEKAQ 268
E+K N +E EKK + +N N++ E KKQ + ++ E+K Q
Sbjct: 1129 EKKKNEEE--EKKKQEEKNKKNEDEEKKKQEEEEKKKNEEEEKKKQ 1172
>sp|P24788|CD2L1_MOUSE PITSLRE serine/threonine-protein kinase CDC2L1
(Galactosyltransferase-associated protein kinase
p58/GTA) (Cell division cycle 2-like protein kinase 1)
Length = 784
Score = 33.5 bits (75), Expect = 0.50
Identities = 22/90 (24%), Positives = 39/90 (43%)
Frame = +2
Query: 176 DSENVDNDEPETKKQRLSDLNDIKDSEEKAQNGDDAEEDQXXXXXXXXXXXXXXXXXXXS 355
D+ +E ++ LSDL DI DSE K + + + + S
Sbjct: 247 DNRKPVKEEKVEERDLLSDLQDISDSERKTSSAESSSAES----------------GSGS 290
Query: 356 EKGTEEHVKEKDCNGSSAKVTENGDSAENE 445
E+ EE +E++ GS+++ +E + E E
Sbjct: 291 EEEEEEEEEEEEEEGSTSEESEEEEEEEEE 320
>sp|P46916|YTXO_BACSU Hypothetical protein ytxO (ORFY)
Length = 143
Score = 33.5 bits (75), Expect = 0.50
Identities = 21/68 (30%), Positives = 37/68 (54%)
Frame = +2
Query: 92 LDMSTVENITPVTEEKNNLKEVIEKKTVDSENVDNDEPETKKQRLSDLNDIKDSEEKAQN 271
LD+ ++ + + K KE EK+T D + VD DE ETK + + + +DS+ A
Sbjct: 45 LDVGSIRELPHINAPKEYQKETKEKET-DRKIVD-DEEETKFETTLEPEEERDSKRSAPP 102
Query: 272 GDDAEEDQ 295
++ +ED+
Sbjct: 103 YEEEDEDE 110
>sp|Q6FSW2|RAD52_CANGA DNA repair and recombination protein RAD52
Length = 505
Score = 33.1 bits (74), Expect = 0.65
Identities = 13/32 (40%), Positives = 20/32 (62%)
Frame = +2
Query: 179 SENVDNDEPETKKQRLSDLNDIKDSEEKAQNG 274
S +DND P K+Q+L++ N I D+ + NG
Sbjct: 181 SNTIDNDNPAVKRQKLNNQNSIPDTNKVKYNG 212
>sp|Q6DRL5|MBB1A_BRARE Myb-binding protein 1A-like protein
Length = 1269
Score = 32.7 bits (73), Expect = 0.85
Identities = 17/55 (30%), Positives = 28/55 (50%)
Frame = +2
Query: 131 EEKNNLKEVIEKKTVDSENVDNDEPETKKQRLSDLNDIKDSEEKAQNGDDAEEDQ 295
++K LKE E + E+ DNDE + + ++ EE + + DD EED+
Sbjct: 723 DKKRKLKEEDEDDDDEEEDDDNDEGDDDDDDDDEEEGGEEGEESSDSSDDEEEDE 777
>sp|P13469|MODU_DROME DNA-binding protein modulo
Length = 542
Score = 32.3 bits (72), Expect = 1.1
Identities = 18/73 (24%), Positives = 34/73 (46%)
Frame = +2
Query: 77 RVNYCLDMSTVENITPVTEEKNNLKEVIEKKTVDSENVDNDEPETKKQRLSDLNDIKDSE 256
RV + E + P + K + K+ +++ SE ++D E ++ D +D + E
Sbjct: 25 RVTKSTKVQEEETVVPQSPSKKSRKQPVKEVPQFSEEDESDVEEQNDEQPGDDSDFETEE 84
Query: 257 EKAQNGDDAEEDQ 295
D+AEED+
Sbjct: 85 AAGLIDDEAEEDE 97
>sp|Q960E8|TF2H1_DROME TFIIH basal transcription factor complex subunit 1
Length = 585
Score = 32.3 bits (72), Expect = 1.1
Identities = 15/35 (42%), Positives = 21/35 (60%)
Frame = +2
Query: 179 SENVDNDEPETKKQRLSDLNDIKDSEEKAQNGDDA 283
S+ VD DEP++KKQRL + D + GDD+
Sbjct: 361 SDQVDKDEPQSKKQRLMEKIHYVDLGDPILEGDDS 395
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 49,977,298
Number of Sequences: 369166
Number of extensions: 735499
Number of successful extensions: 4297
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3162
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4118
length of database: 68,354,980
effective HSP length: 106
effective length of database: 48,773,070
effective search space used: 5462583840
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)