Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_006_L01
(770 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q05052|OST48_CANFA Dolichyl-diphosphooligosaccharide--pr... 278 9e-75
sp|O54734|OST48_MOUSE Dolichyl-diphosphooligosaccharide--pr... 277 3e-74
sp|P39656|OST48_HUMAN Dolichyl-diphosphooligosaccharide--pr... 275 8e-74
sp|P48440|OST48_CHICK Dolichyl-diphosphooligosaccharide--pr... 275 1e-73
sp|Q24319|OST48_DROME Dolichyl-diphosphooligosaccharide--pr... 254 2e-67
sp|P45971|OST48_CAEEL Probable dolichyl-diphosphooligosacch... 236 4e-62
sp|P33767|OSTB_YEAST Dolichyl-diphosphooligosaccharide--pro... 93 9e-19
sp|O26270|PURQ_METTH Phosphoribosylformylglycinamidine synt... 35 0.29
sp|O78432|YCF30_GUITH Putative HTH-type transcriptional reg... 33 0.86
sp|P34374|UNC36_CAEEL Voltage-dependent calcium channel unc... 31 4.3
>sp|Q05052|OST48_CANFA Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48
kDa subunit precursor (Oligosaccharyl transferase 48 kDa
subunit)
Length = 445
Score = 278 bits (712), Expect = 9e-75
Identities = 140/234 (59%), Positives = 179/234 (76%), Gaps = 1/234 (0%)
Frame = +3
Query: 6 PDKPVDDYPNSVGINTQLITALQARNNARVLFVGSLDLFSDDLFTASVEHAI-SKQTHAR 182
PDKP+ YP++VG NT LI LQARNNARV+F GSLD FSD F ++V+ A Q +++
Sbjct: 215 PDKPITQYPHAVGKNTLLIAGLQARNNARVVFSGSLDFFSDAFFNSAVQKAAPGSQRYSQ 274
Query: 183 SGNEALATAISKWVFKETGVLRVGNVSHSLADKSVENpreYTIMENVHYEIVIDELRADG 362
+GN LA A+S+WVFKE GVLRVG VSH ++ P YT+ + V Y IVI++L ++G
Sbjct: 275 TGNYELAVALSRWVFKEEGVLRVGPVSHHRVGETAP-PNAYTVTDLVEYSIVIEQL-SNG 332
Query: 363 TWTPFIADDVQLEFYRIDPFVRQNMLRKEGKFYLDFKLPDVYGVFKFLVDYHRVGYTHVH 542
W PF DD+QLEF RIDPFVR + +K GK+ + FKLPDVYGVF+F VDY+R+GYTH+H
Sbjct: 333 RWVPFDGDDIQLEFVRIDPFVRTFLKKKGGKYSVQFKLPDVYGVFQFKVDYNRLGYTHLH 392
Query: 543 SSTQVSVRPLMHTQYERFIQAAYPYYASAISMMVGVILFSFLFLYFKDDKEKNE 704
SSTQVSVRPL HTQYERFI +AYPYYASA SMM+G+ +FS +FL+ K +KEK++
Sbjct: 393 SSTQVSVRPLQHTQYERFIPSAYPYYASAFSMMLGLFIFSTVFLHMK-EKEKSD 445
>sp|O54734|OST48_MOUSE Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48
kDa subunit precursor (Oligosaccharyl transferase 48 kDa
subunit) (DDOST 48 kDa subunit)
Length = 441
Score = 277 bits (708), Expect = 3e-74
Identities = 140/234 (59%), Positives = 177/234 (75%), Gaps = 1/234 (0%)
Frame = +3
Query: 6 PDKPVDDYPNSVGINTQLITALQARNNARVLFVGSLDLFSDDLFTASVEHAI-SKQTHAR 182
PDKP+ YP++VG NT LI LQARNNARV+F GSLD FSD F ++V+ A Q +++
Sbjct: 211 PDKPITQYPHAVGRNTLLIAGLQARNNARVIFSGSLDFFSDAFFNSAVQKATPGAQRYSQ 270
Query: 183 SGNEALATAISKWVFKETGVLRVGNVSHSLADKSVENpreYTIMENVHYEIVIDELRADG 362
+GN LA A+S+WVFKE GVLRVG VSH + P YT+ + V Y IVI++L ++G
Sbjct: 271 TGNYELAVALSRWVFKEEGVLRVGPVSHHRVGEMAP-PNAYTVTDLVEYSIVIEQL-SNG 328
Query: 363 TWTPFIADDVQLEFYRIDPFVRQNMLRKEGKFYLDFKLPDVYGVFKFLVDYHRVGYTHVH 542
W PF DD+QLEF RIDPFVR + RK GK+ + FKLPDVYGVF+F VDY+R+GYTH++
Sbjct: 329 KWVPFDGDDIQLEFVRIDPFVRTFLKRKGGKYSVQFKLPDVYGVFQFKVDYNRLGYTHLY 388
Query: 543 SSTQVSVRPLMHTQYERFIQAAYPYYASAISMMVGVILFSFLFLYFKDDKEKNE 704
SSTQVSVRPL HTQYERFI +AYPYYASA SMM G+ +FS +FL+ K +KEK++
Sbjct: 389 SSTQVSVRPLQHTQYERFIPSAYPYYASAFSMMAGLFIFSIVFLHMK-EKEKSD 441
>sp|P39656|OST48_HUMAN Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48
kDa subunit precursor (Oligosaccharyl transferase 48 kDa
subunit) (DDOST 48 kDa subunit)
Length = 439
Score = 275 bits (704), Expect = 8e-74
Identities = 138/234 (58%), Positives = 177/234 (75%), Gaps = 1/234 (0%)
Frame = +3
Query: 6 PDKPVDDYPNSVGINTQLITALQARNNARVLFVGSLDLFSDDLFTASVEHAI-SKQTHAR 182
PDKP+ YP++VG NT LI LQARNNARV+F GSLD FSD F ++V+ A Q +++
Sbjct: 209 PDKPITQYPHAVGKNTLLIAGLQARNNARVIFSGSLDFFSDSFFNSAVQKAAPGSQRYSQ 268
Query: 183 SGNEALATAISKWVFKETGVLRVGNVSHSLADKSVENpreYTIMENVHYEIVIDELRADG 362
+GN LA A+S+WVFKE GVLRVG VSH ++ P YT+ + V Y IVI +L ++G
Sbjct: 269 TGNYELAVALSRWVFKEEGVLRVGPVSHHRVGETAP-PNAYTVTDLVEYSIVIQQL-SNG 326
Query: 363 TWTPFIADDVQLEFYRIDPFVRQNMLRKEGKFYLDFKLPDVYGVFKFLVDYHRVGYTHVH 542
W PF DD+QLEF RIDPFVR + +K GK+ + FKLPDVYGVF+F VDY+R+GYTH++
Sbjct: 327 KWVPFDGDDIQLEFVRIDPFVRTFLKKKGGKYSVQFKLPDVYGVFQFKVDYNRLGYTHLY 386
Query: 543 SSTQVSVRPLMHTQYERFIQAAYPYYASAISMMVGVILFSFLFLYFKDDKEKNE 704
SSTQVSVRPL HTQYERFI +AYPYYASA MM+G+ +FS +FL+ K +KEK++
Sbjct: 387 SSTQVSVRPLQHTQYERFIPSAYPYYASAFPMMLGLFIFSIVFLHMK-EKEKSD 439
>sp|P48440|OST48_CHICK Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48
kDa subunit (Oligosaccharyl transferase 48 kDa subunit)
(OST 50 kDa subunit) (DDOST 48 kDa subunit)
Length = 413
Score = 275 bits (703), Expect = 1e-73
Identities = 139/234 (59%), Positives = 178/234 (76%), Gaps = 1/234 (0%)
Frame = +3
Query: 6 PDKPVDDYPNSVGINTQLITALQARNNARVLFVGSLDLFSDDLFTASVEHAI-SKQTHAR 182
PDKP+ YP++VG NT LI LQARNNARV+F GSLD FSD F+++V+ A + +++
Sbjct: 183 PDKPITQYPHAVGKNTLLIAGLQARNNARVVFSGSLDFFSDAFFSSAVQKAAPGSKRYSQ 242
Query: 183 SGNEALATAISKWVFKETGVLRVGNVSHSLADKSVENpreYTIMENVHYEIVIDELRADG 362
+GN LA A+S+WVFKE GVLRVG VSH + + P YT+ + V Y IVI++L +DG
Sbjct: 243 TGNYELAVALSRWVFKEEGVLRVGAVSHHRVGE-LAPPNAYTVTDLVEYSIVIEKL-SDG 300
Query: 363 TWTPFIADDVQLEFYRIDPFVRQNMLRKEGKFYLDFKLPDVYGVFKFLVDYHRVGYTHVH 542
W PF DD+QLEF RIDPFVR + R GK+ + FKLPDVYGVF+F VDY+R+GYTH++
Sbjct: 301 KWIPFDGDDIQLEFVRIDPFVRTFLKRNGGKYSVQFKLPDVYGVFQFKVDYNRLGYTHLY 360
Query: 543 SSTQVSVRPLMHTQYERFIQAAYPYYASAISMMVGVILFSFLFLYFKDDKEKNE 704
SSTQVSVRPL HTQYERFI +AYPYYA A SMMVG+ +FS +FL+ K +KEK++
Sbjct: 361 SSTQVSVRPLQHTQYERFIPSAYPYYAGAFSMMVGLFMFSIVFLHMK-EKEKSD 413
>sp|Q24319|OST48_DROME Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48
kDa subunit precursor (Oligosaccharyl transferase 48 kDa
subunit) (DDOST 48 kDa subunit) (OST5OP) (DrOST)
(DmOST50)
Length = 449
Score = 254 bits (648), Expect = 2e-67
Identities = 131/230 (56%), Positives = 166/230 (72%), Gaps = 1/230 (0%)
Frame = +3
Query: 3 NPDKPVDDYPNSVGINTQLITALQARNNARVLFVGSLDLFSDDLFTASVEHAISKQTHAR 182
NP+ V DYP++VG T LI ALQARNNARV+F GSL FSD+ FT +V++A S H
Sbjct: 208 NPEASVSDYPHAVGRGTLLIAALQARNNARVVFSGSLLFFSDESFTTAVQYAQSGVFHKL 267
Query: 183 SGNEALATAISKWVFKETGVLRVGNVSHSLADKSVENpreYTIMENVHYEIVIDELRADG 362
+GN +A +ISKWVF ETG LRV +V H + + + YTI + V Y I I+EL G
Sbjct: 268 AGNRDVAESISKWVFGETGRLRVASVQHHKEGELLPPDQAYTITDPVVYTIGIEEL-VQG 326
Query: 363 TWTPFIADDVQLEFYRIDPFVRQNMLRKE-GKFYLDFKLPDVYGVFKFLVDYHRVGYTHV 539
W F A D+QLEF RIDPFVR + + G + FK+PDVYGV++F VDY+RVGYTH+
Sbjct: 327 EWRAFKASDIQLEFVRIDPFVRTYLKQTNTGAYQAKFKIPDVYGVYQFKVDYNRVGYTHL 386
Query: 540 HSSTQVSVRPLMHTQYERFIQAAYPYYASAISMMVGVILFSFLFLYFKDD 689
+S+TQVSVRPL HTQYERFI +A+PYY SA SMM+GV +FSF+FL+FKD+
Sbjct: 387 YSTTQVSVRPLEHTQYERFIPSAFPYYTSAFSMMIGVFVFSFVFLHFKDE 436
>sp|P45971|OST48_CAEEL Probable dolichyl-diphosphooligosaccharide--protein
glycosyltransferase 48 kDa subunit precursor
(Oligosaccharyl transferase 48 kDa subunit)
Length = 445
Score = 236 bits (603), Expect = 4e-62
Identities = 121/223 (54%), Positives = 152/223 (68%)
Frame = +3
Query: 30 PNSVGINTQLITALQARNNARVLFVGSLDLFSDDLFTASVEHAISKQTHARSGNEALATA 209
P+ G T L+ LQ+RNNAR++F GS +LFS+ F+A A+SGN ATA
Sbjct: 212 PSIAGSRTLLVGGLQSRNNARIVFTGSSELFSNTFFSAKTNSVNPSVQGAQSGNADFATA 271
Query: 210 ISKWVFKETGVLRVGNVSHSLADKSVENpreYTIMENVHYEIVIDELRADGTWTPFIADD 389
I++WV KE+GVLRV V+H ++V Y I E+V Y I I+EL+ +G W PF D
Sbjct: 272 ITRWVMKESGVLRVKTVNHHKKGETVPPVEGYFITEDVVYTIEIEELK-NGKWVPFQGKD 330
Query: 390 VQLEFYRIDPFVRQNMLRKEGKFYLDFKLPDVYGVFKFLVDYHRVGYTHVHSSTQVSVRP 569
VQLEF RIDPFVR + G+ + FKLPDV GVFKFLVDY RVGYTH++ QVSVRP
Sbjct: 331 VQLEFVRIDPFVRATLKNSNGRLSVAFKLPDVLGVFKFLVDYRRVGYTHLYDVQQVSVRP 390
Query: 570 LMHTQYERFIQAAYPYYASAISMMVGVILFSFLFLYFKDDKEK 698
L HTQYERFI++AYPYYAS+ SMM G++LFS ++LY KD K
Sbjct: 391 LWHTQYERFIRSAYPYYASSFSMMAGLVLFSIVYLYHKDTPVK 433
>sp|P33767|OSTB_YEAST Dolichyl-diphosphooligosaccharide--protein glycosyltransferase beta
subunit precursor (Oligosaccharyl transferase beta
subunit)
Length = 430
Score = 92.8 bits (229), Expect = 9e-19
Identities = 60/214 (28%), Positives = 100/214 (46%), Gaps = 8/214 (3%)
Frame = +3
Query: 57 LITALQARNNARVLFVGSLDLFSDDLFTASVEHAISKQTHARSGNEALATAISKWVFKET 236
L+ Q NNAR++++GS D + + N+ A + KW F E
Sbjct: 217 LVVGFQNLNNARLVWIGSSDFLKNK---------------NQDSNQEFAKELLKWTFNEK 261
Query: 237 GVLRVGNVSHSLADKSVENpreYTIMENVHYEIVIDELRADGTWTPFIADDVQLEFYRID 416
V++ + HS AD + + Y I + V Y + E + W P IADD+Q E ++D
Sbjct: 262 SVIKSVHAVHSHADGTSYDEEPYKIKDKVIYSVGFSEWNGE-EWLPHIADDIQFELRQVD 320
Query: 417 PFVRQNMLRK----EGKFYL--DFKLPDVYGVFKFLVDYHRVGYTHVHSSTQVSVRPLMH 578
P+ R + E ++Y +F LPD +GVF FL DY ++G + ++R L +
Sbjct: 321 PYYRLTLSPSGNDSETQYYTTGEFILPDRHGVFTFLTDYRKIGLSFTTDKDVKAIRHLAN 380
Query: 579 TQYERF--IQAAYPYYASAISMMVGVILFSFLFL 674
+Y R I ++ Y ++ ++V I F F+
Sbjct: 381 DEYPRSWEISNSWVYISAICGVIVAWIFFVVSFV 414
>sp|O26270|PURQ_METTH Phosphoribosylformylglycinamidine synthase I (FGAM synthase I)
Length = 218
Score = 34.7 bits (78), Expect = 0.29
Identities = 33/135 (24%), Positives = 60/135 (44%), Gaps = 4/135 (2%)
Frame = +3
Query: 126 DDLFTASVEHAISKQTHARSGNEALAT----AISKWVFKETGVLRVGNVSHSLADKSVEN 293
D L + S + R+G A T A+ + V +E VL + N + LA+ +
Sbjct: 39 DHLDAVVIPGGFSYGDYLRAGAIAAITPVMDAVRELVREEKPVLGICNGAQILAEVGLV- 97
Query: 294 preYTIMENVHYEIVIDELRADGTWTPFIADDVQLEFYRIDPFVRQNMLRKEGKFYLDFK 473
P +T+ E+ + ELR T TPF ++ D +R + EG++Y D
Sbjct: 98 PGVFTVNEHPKFNCQWTELRVKTTRTPFTG------LFKKDEVIRMPVAHAEGRYYHD-N 150
Query: 474 LPDVYGVFKFLVDYH 518
+ +V+ + ++ +H
Sbjct: 151 ISEVWENDQVVLQFH 165
>sp|O78432|YCF30_GUITH Putative HTH-type transcriptional regulator ycf30
Length = 310
Score = 33.1 bits (74), Expect = 0.86
Identities = 17/59 (28%), Positives = 32/59 (54%)
Frame = +3
Query: 336 VIDELRADGTWTPFIADDVQLEFYRIDPFVRQNMLRKEGKFYLDFKLPDVYGVFKFLVD 512
V DELR+ TP++ D++ L ++ PF + +++KE + L F D + ++D
Sbjct: 155 VPDELRSKLQITPYVDDELALILPKLHPFSKLEVIQKEDLYRLRFITLDKQSTIRRVID 213
>sp|P34374|UNC36_CAEEL Voltage-dependent calcium channel unc-36 precursor (Uncoordinated
protein 36)
Length = 1249
Score = 30.8 bits (68), Expect = 4.3
Identities = 14/33 (42%), Positives = 20/33 (60%), Gaps = 2/33 (6%)
Frame = -1
Query: 392 DIISNERSPCSISPQLVNNDLVMH--ILHYCVF 300
D NER PCS SP +V+ ++ LH+C+F
Sbjct: 1217 DDSENERRPCSTSPTIVSIFQILFGVFLHFCIF 1249
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 85,999,753
Number of Sequences: 369166
Number of extensions: 1732756
Number of successful extensions: 5103
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4919
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5081
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 7163732800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)