Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_006_K05
(501 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9U4L6|TOM40_DROME Probable mitochondrial import recepto... 87 2e-17
sp|Q9QYA2|TOM40_MOUSE Probable mitochondrial import recepto... 80 3e-15
sp|O96008|TOM40_HUMAN Probable mitochondrial import recepto... 77 3e-14
sp|Q18090|TOM40_CAEEL Probable mitochondrial import recepto... 72 1e-12
sp|O13656|TOM40_SCHPO Probable mitochondrial import recepto... 40 0.004
sp|P19812|UBR1_YEAST Ubiquitin-protein ligase E3 component ... 31 1.4
sp|Q9URQ3|TAD3_YEAST tRNA-specific adenosine deaminase 3 (t... 30 4.2
sp|P27571|XIST_MOUSE X inactive-specific transcript protein 30 4.2
sp|P61872|LIP_RHIOR Lipase precursor (Triacylglycerol lipas... 28 9.3
>sp|Q9U4L6|TOM40_DROME Probable mitochondrial import receptor subunit TOM40 homolog
(Translocase of outer membrane 40 kDa subunit homolog)
(Male sterile protein 15)
Length = 344
Score = 87.4 bits (215), Expect = 2e-17
Identities = 48/144 (33%), Positives = 77/144 (53%), Gaps = 2/144 (1%)
Frame = +3
Query: 21 ITDKLSLGSMFVFQRSNQAQFTNFGVWNVGGQYVGNK--WTAAVGVRPFQSSLHSTFHMK 194
+T L+LGS +Q + +V G+Y W+ +G QS LH ++ K
Sbjct: 205 VTPALALGSELAYQFGPNVPGRQIAIMSVVGRYTAGSSVWSGTLG----QSGLHVCYYQK 260
Query: 195 LNEAFQIGTEIETSLGHRQCVGTAGYQFDFPKSGITIKSQVDSNWKIATSYERKFIAPIP 374
++ QIG E+ETSL ++ V T YQ D PK+ + + +DSNW+I E++ +AP+P
Sbjct: 261 ASDQLQIGAEVETSLRMQESVATLAYQIDLPKANLVFRGGIDSNWQIFGVLEKR-LAPLP 319
Query: 375 FSINLCINGDIWKSNYGLGVGFSI 446
F++ L + K+N+ LG G I
Sbjct: 320 FTLALSGRMNHVKNNFRLGCGLMI 343
>sp|Q9QYA2|TOM40_MOUSE Probable mitochondrial import receptor subunit TOM40 homolog
(Translocase of outer membrane 40 kDa subunit homolog)
Length = 359
Score = 80.1 bits (196), Expect = 3e-15
Identities = 45/142 (31%), Positives = 76/142 (53%)
Frame = +3
Query: 21 ITDKLSLGSMFVFQRSNQAQFTNFGVWNVGGQYVGNKWTAAVGVRPFQSSLHSTFHMKLN 200
IT L+LG V+ R + T V ++ G+Y N W A V + Q+ +H+T++ K +
Sbjct: 223 ITPCLALGGELVYHRRPGEEGT---VMSLAGKYTLNNWLATVTLG--QAGMHATYYHKAS 277
Query: 201 EAFQIGTEIETSLGHRQCVGTAGYQFDFPKSGITIKSQVDSNWKIATSYERKFIAPIPFS 380
+ Q+G E E S + + GYQ D PK+ K V+SNW + + E+K + P+P +
Sbjct: 278 DQLQVGVEFEASTRMQDTSASFGYQLDLPKANFLFKGSVNSNWIVGATLEKK-LPPLPLT 336
Query: 381 INLCINGDIWKSNYGLGVGFSI 446
++LC + K+ + G G +I
Sbjct: 337 LSLCAFLNHRKNKFLCGFGLTI 358
>sp|O96008|TOM40_HUMAN Probable mitochondrial import receptor subunit TOM40 homolog
(Translocase of outer membrane 40 kDa subunit homolog)
(Haymaker protein) (p38.5)
Length = 361
Score = 76.6 bits (187), Expect = 3e-14
Identities = 45/142 (31%), Positives = 75/142 (52%)
Frame = +3
Query: 21 ITDKLSLGSMFVFQRSNQAQFTNFGVWNVGGQYVGNKWTAAVGVRPFQSSLHSTFHMKLN 200
IT L+LG V+ R + T V ++ G+Y N W A V + Q+ +H+T++ K +
Sbjct: 225 ITPCLALGGELVYHRRPGEEGT---VMSLAGKYTLNNWLATVTLG--QAGMHATYYHKAS 279
Query: 201 EAFQIGTEIETSLGHRQCVGTAGYQFDFPKSGITIKSQVDSNWKIATSYERKFIAPIPFS 380
+ Q+G E E S + + GYQ D PK+ + K VDSNW + + E+K + P+P +
Sbjct: 280 DQLQVGVEFEASTRMQDTSVSFGYQLDLPKANLLFKGSVDSNWIVGATLEKK-LPPLPLT 338
Query: 381 INLCINGDIWKSNYGLGVGFSI 446
+ L + K+ + G G +I
Sbjct: 339 LALGAFLNHRKNKFQCGFGLTI 360
>sp|Q18090|TOM40_CAEEL Probable mitochondrial import receptor subunit TOM40 homolog
(Translocase of outer membrane 40 kDa subunit homolog)
Length = 301
Score = 71.6 bits (174), Expect = 1e-12
Identities = 39/144 (27%), Positives = 70/144 (48%)
Frame = +3
Query: 15 RRITDKLSLGSMFVFQRSNQAQFTNFGVWNVGGQYVGNKWTAAVGVRPFQSSLHSTFHMK 194
RR+T +L +G+ V+Q V + +Y N + AA + S +H T++ K
Sbjct: 159 RRLTPRLDVGTEMVYQYGKNIPGGQISVLSYAARYTANHFIAAATLGA--SGVHLTYYHK 216
Query: 195 LNEAFQIGTEIETSLGHRQCVGTAGYQFDFPKSGITIKSQVDSNWKIATSYERKFIAPIP 374
NE G E E + + V T YQ + P+ G+T+++ D+NW + +E++ +P
Sbjct: 217 QNENLAFGVEFECNANVGEAVTTLAYQTELPEEGVTMRASFDTNWTVGGVFEKRLSQQLP 276
Query: 375 FSINLCINGDIWKSNYGLGVGFSI 446
F++ L + K+ G+G I
Sbjct: 277 FTLALSGTLNHVKAAGKFGIGLII 300
>sp|O13656|TOM40_SCHPO Probable mitochondrial import receptor subunit tom40 (Translocase
of outer membrane 40 kDa subunit)
Length = 344
Score = 39.7 bits (91), Expect = 0.004
Identities = 34/154 (22%), Positives = 64/154 (41%), Gaps = 12/154 (7%)
Frame = +3
Query: 21 ITDKLSLGSMFVFQRSNQAQFTNFGVWNVGGQYVGNKWTAAVGVRPFQSSLHSTFHMKLN 200
+T KLSLG ++Q+ + + + +Y W A + Q + +TF KL+
Sbjct: 174 VTPKLSLGVEALWQKPSSSIGPEEATLSYMTRYNAADWIATAHLNGSQGDVTATFWRKLS 233
Query: 201 EAFQIGTEIETS---LGHRQCVGT---------AGYQFDFPKSGITIKSQVDSNWKIATS 344
+ G E + S L H + T G +++F +S + QVDS ++
Sbjct: 234 PKVEAGVECQLSPVGLNHSAALMTGPKPEGLTSVGVKYEFAQS--IYRGQVDSKGRVGVY 291
Query: 345 YERKFIAPIPFSINLCINGDIWKSNYGLGVGFSI 446
ER+ I + + ++ + GLG+ +
Sbjct: 292 LERRLAPAITLAFSSELDHPNRNAKVGLGLSLEL 325
>sp|P19812|UBR1_YEAST Ubiquitin-protein ligase E3 component N-recognin-1 (N-end-recognizing
protein)
Length = 1950
Score = 31.2 bits (69), Expect = 1.4
Identities = 17/71 (23%), Positives = 35/71 (49%)
Frame = -2
Query: 296 TRFREIKLITSCTHALTMTQTSLNFCTYLKCFIQLHVKS*M*RTLERPNSHCRSPFITHI 117
T+ +EIKL + + L+F L C +++H+++ + N +C PF +
Sbjct: 1676 TQSKEIKLREERSQHMKNADNRLDFKICLTCGVKVHLRADRHEMTKHLNKNCFKPFGAFL 1735
Query: 116 LPTNIPYTEIC 84
+P + +E+C
Sbjct: 1736 MPNS---SEVC 1743
>sp|Q9URQ3|TAD3_YEAST tRNA-specific adenosine deaminase 3 (tRNA-specific adenosine-34
deaminase subunit TAD3)
Length = 322
Score = 29.6 bits (65), Expect = 4.2
Identities = 14/32 (43%), Positives = 19/32 (59%)
Frame = -3
Query: 100 HTPKFVNCA*LLLWKTNIDPRDNLSVILLVPN 5
+TPK +N +W IDPRD+ VI L+ N
Sbjct: 32 NTPKLIN-----VWSIRIDPRDSKKVIELIRN 58
>sp|P27571|XIST_MOUSE X inactive-specific transcript protein
Length = 298
Score = 29.6 bits (65), Expect = 4.2
Identities = 16/58 (27%), Positives = 26/58 (44%), Gaps = 1/58 (1%)
Frame = -2
Query: 374 WYRSYKLSLVRCSDFPITINLRFDCNTRFREIKLITSCTH-ALTMTQTSLNFCTYLKC 204
W L++ C + + I+L F FR+ +T C H +L + T L C+ C
Sbjct: 129 WSALSSLAMALCFIYLVAISLSFSFTPAFRDSLWVTCCMHPSLFLVLTPLVLCSSSLC 186
>sp|P61872|LIP_RHIOR Lipase precursor (Triacylglycerol lipase) (ROL) (RDL)
sp|P61871|LIP_RHINI Lipase precursor (Triacylglycerol lipase) (Lipase II) (RNL)
Length = 392
Score = 28.5 bits (62), Expect = 9.3
Identities = 16/50 (32%), Positives = 26/50 (52%), Gaps = 4/50 (8%)
Frame = +3
Query: 39 LGSMFVFQRSNQAQFTNFGVWNVGGQYVGNK----WTAAVGVRPFQSSLH 176
L M ++QR + N ++ VGG VGN + + G+ PFQ ++H
Sbjct: 276 LAGMDLYQREPRLSPKNLSIFTVGGPRVGNPTFAYYVESTGI-PFQRTVH 324
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 60,559,611
Number of Sequences: 369166
Number of extensions: 1229888
Number of successful extensions: 2478
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 2429
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2474
length of database: 68,354,980
effective HSP length: 102
effective length of database: 49,512,010
effective search space used: 3168768640
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)