Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_006_I05
(872 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P07602|SAP_HUMAN Proactivator polypeptide precursor [Con... 69 2e-11
sp|O13035|SAP_CHICK Proactivator polypeptide precursor [Con... 68 3e-11
sp|P26779|SAP_BOVIN Proactivator polypeptide precursor [Con... 68 3e-11
sp|P42211|ASPRX_ORYSA Aspartic proteinase precursor 47 5e-05
sp|P10960|SAP_RAT Sulfated glycoprotein 1 precursor (SGP-1)... 47 9e-05
sp|P20097|SAP_CAVPO Saposin C (CO-beta-glucosidase) (Glucos... 45 3e-04
sp|P50405|PSPB_MOUSE Pulmonary surfactant-associated protei... 44 8e-04
sp|Q61207|SAP_MOUSE Sulfated glycoprotein 1 precursor (SGP-... 43 0.001
sp|P17129|PSPB_CANFA Pulmonary surfactant-associated protei... 41 0.005
sp|P22355|PSPB_RAT Pulmonary surfactant-associated protein ... 40 0.008
>sp|P07602|SAP_HUMAN Proactivator polypeptide precursor [Contains: Saposin A (Protein
A); Saposin B-Val; Saposin B (Sphingolipid activator
protein 1) (SAP-1) (Cerebroside sulfate activator)
(CSAct) (Dispersin) (Sulfatide/GM1 activator); Saposin C
(Co-beta-glucosidase) (A1 activator) (Glucosylceramidase
activator) (Sphingolipid activator protein 2) (SAP-2);
Saposin D (Protein C) (Component C)]
Length = 524
Score = 68.9 bits (167), Expect = 2e-11
Identities = 51/186 (27%), Positives = 96/186 (51%), Gaps = 6/186 (3%)
Frame = +1
Query: 202 SGIECELCEHVMDEVIKVVTSNHSEEVVSNFIKLKICPRL-SVIKNECVIIVDTYLPVIF 378
S + CE+CE ++ EV K++ +N +E+ + + K+C +L + EC +VDTY I
Sbjct: 311 SDVYCEVCEFLVKEVTKLIDNNKTEKEILDAFD-KMCSKLPKSLSEECQEVVDTYGSSIL 369
Query: 379 SIARSTLNSKVLCTELKLC--TLLSKKKISFLMNRSGNETLKCEICRFAILEV-RTLLTD 549
SI ++ +++C+ L LC T L + + G CE+C+ + + R L +
Sbjct: 370 SILLEEVSPELVCSMLHLCSGTRLPALTVHVTQPKDGG---FCEVCKKLVGYLDRNLEKN 426
Query: 550 PGFQKIFENFLINEICPHFKTK-QQQCTNDINQYYSLVVNIIVEFLNPS-LCSELGLCNS 723
Q+I + + C Q+QC + +Y +++ I+VE ++PS +C ++G C S
Sbjct: 427 STKQEILA--ALEKGCSFLPDPYQKQCDQFVAEYEPVLIEILVEVMDPSFVCLKIGACPS 484
Query: 724 TIKQVI 741
K ++
Sbjct: 485 AHKPLL 490
Score = 45.1 bits (105), Expect = 3e-04
Identities = 35/155 (22%), Positives = 68/155 (43%), Gaps = 13/155 (8%)
Frame = +1
Query: 10 ISDPATETEIKNFLKNDFCYIFGSDKIQCQQIVDEYFNLILNFILAEVDVDKICKFFGLC 189
I + TE EI + + S +CQ++VD Y + IL+ +L EV + +C LC
Sbjct: 329 IDNNKTEKEILDAFDKMCSKLPKSLSEECQEVVDTYGSSILSILLEEVSPELVCSMLHLC 388
Query: 190 NA------------KLSGIECELCEHVMDEVIKVVTSNHSEEVVSNFIKLKICPRL-SVI 330
+ G CE+C+ ++ + + + N +++ + ++ K C L
Sbjct: 389 SGTRLPALTVHVTQPKDGGFCEVCKKLVGYLDRNLEKNSTKQEILAALE-KGCSFLPDPY 447
Query: 331 KNECVIIVDTYLPVIFSIARSTLNSKVLCTELKLC 435
+ +C V Y PV+ I ++ +C ++ C
Sbjct: 448 QKQCDQFVAEYEPVLIEILVEVMDPSFVCLKIGAC 482
Score = 41.6 bits (96), Expect = 0.003
Identities = 47/228 (20%), Positives = 97/228 (42%), Gaps = 45/228 (19%)
Frame = +1
Query: 199 LSGIECELCEHVMDEVIKVVTSNHSEEVVSNFIKLKICPRLSV--IKNECVIIVDTYLPV 372
+ + C++C+ V+ ++ N +EE + +++ K C L + C IVD+YLPV
Sbjct: 58 VKSLPCDICKDVVTAAGDMLKDNATEEEILVYLE-KTCDWLPKPNMSASCKEIVDSYLPV 116
Query: 373 IFSIARSTLNSK-VLCTELKLCTLLSKKKISFLMNRSGNETLK----------------- 498
I I + ++ +C+ L LC L +K ++ L ++ E+ K
Sbjct: 117 ILDIIKGEMSRPGEVCSALNLCESL-QKHLAELNHQKQLESNKIPELDMTEVVAPFMANI 175
Query: 499 ----------------------CEICRFAILEVRTLL-TDPGF-QKIFENFLINEICPHF 606
C+ C + +++T + T+ F Q + E+ + E C
Sbjct: 176 PLLLYPQDGPRSKPQPKDNGDVCQDCIQMVTDIQTAVRTNSTFVQALVEH--VKEECDRL 233
Query: 607 KTKQQQ-CTNDINQYYSLVVNIIVEFLNPSLCSELGLCNSTIKQVIMR 747
C N I+QY + + +++ +C+ +G C+ +K++ M+
Sbjct: 234 GPGMADICKNYISQYSEIAIQMMMHMQPKEICALVGFCDE-VKEMPMQ 280
Score = 31.6 bits (70), Expect = 3.0
Identities = 19/66 (28%), Positives = 34/66 (51%), Gaps = 2/66 (3%)
Frame = +1
Query: 4 EYISDPATETEIKNFLKNDFCYIFGSD-KIQCQQIVDEYFNLILNFILAEVD-VDKICKF 177
+ + D ATE EI +L+ ++ + C++IVD Y +IL+ I E+ ++C
Sbjct: 75 DMLKDNATEEEILVYLEKTCDWLPKPNMSASCKEIVDSYLPVILDIIKGEMSRPGEVCSA 134
Query: 178 FGLCNA 195
LC +
Sbjct: 135 LNLCES 140
>sp|O13035|SAP_CHICK Proactivator polypeptide precursor [Contains: Saposin A; Saposin B;
Saposin C; Saposin D]
Length = 518
Score = 68.2 bits (165), Expect = 3e-11
Identities = 52/250 (20%), Positives = 107/250 (42%), Gaps = 34/250 (13%)
Frame = +1
Query: 94 CQQIVDEYFNLILNFILAEVDVDK--ICKFFGLC------------------NAKLSGIE 213
C+ + EY +L + ++ D IC G C K+ +E
Sbjct: 240 CKSYISEYSDLAIQMMMHMKDQQPKDICAMVGFCPSVKSVPLQTLVPAQVVHEVKMETVE 299
Query: 214 -----------CELCEHVMDEVIKVVTSNHSEEVVSNFIKLKICPRLSVIKNECVIIVDT 360
CE+CE ++ EV ++ SN +EE + + +++ + +K++C ++
Sbjct: 300 KATVQEKTFSVCEICETMVKEVTGLLESNKTEEEIVHEMEVVCYLLPASVKDQCKDFIEV 359
Query: 361 YLPVIFSIARSTLNSKVLCTELKLCTLLSKKKISFLMNRSGNETLKCEICRFAILEVRTL 540
Y + + N + +C LK C + ++ +G C+IC+ +
Sbjct: 360 YGQALIDMLLEATNPEAVCVMLKCCAANKPPQQPVVVKPAGG---FCDICKMIVAYADKE 416
Query: 541 LTDPGFQKIFENFLINEICPHF--KTKQQQCTNDINQYYSLVVNIIVEFLNPS-LCSELG 711
L E L+ ++C HF ++ QC + QY +VV ++ E ++P+ +C++LG
Sbjct: 417 LEKNATTTEIE-ALLEKVC-HFLPESVSDQCVQFVEQYEPVVVQLLAEMMDPTFVCTKLG 474
Query: 712 LCNSTIKQVI 741
+C + K ++
Sbjct: 475 VCGAAKKPLL 484
Score = 50.8 bits (120), Expect = 5e-06
Identities = 29/87 (33%), Positives = 52/87 (59%), Gaps = 3/87 (3%)
Frame = +1
Query: 199 LSGIECELCEHVMDEVIKVVTSNHSEEVVSNFIKLKICPRL--SVIKNECVIIVDTYLPV 372
++ I C+LC+ ++ V KV+ N +E+ + ++++ K C L + +EC IVD+YLPV
Sbjct: 59 VNSIPCDLCKELVTVVGKVLKDNGTEDEIRSYLE-KRCEFLPDQGLASECKEIVDSYLPV 117
Query: 373 IFSIARSTLNS-KVLCTELKLCTLLSK 450
I + + + +V+C+ L LC L K
Sbjct: 118 IMDMIKEEFDKPEVVCSALSLCQSLQK 144
Score = 33.5 bits (75), Expect = 0.79
Identities = 19/65 (29%), Positives = 34/65 (52%), Gaps = 3/65 (4%)
Frame = +1
Query: 10 ISDPATETEIKNFLKNDFCYIFGSDKI--QCQQIVDEYFNLILNFILAEVD-VDKICKFF 180
+ D TE EI+++L+ C + +C++IVD Y +I++ I E D + +C
Sbjct: 78 LKDNGTEDEIRSYLEKR-CEFLPDQGLASECKEIVDSYLPVIMDMIKEEFDKPEVVCSAL 136
Query: 181 GLCNA 195
LC +
Sbjct: 137 SLCQS 141
Score = 33.1 bits (74), Expect = 1.0
Identities = 17/58 (29%), Positives = 30/58 (51%)
Frame = +1
Query: 22 ATETEIKNFLKNDFCYIFGSDKIQCQQIVDEYFNLILNFILAEVDVDKICKFFGLCNA 195
AT TEI+ L+ ++ S QC Q V++Y +++ + +D +C G+C A
Sbjct: 421 ATTTEIEALLEKVCHFLPESVSDQCVQFVEQYEPVVVQLLAEMMDPTFVCTKLGVCGA 478
>sp|P26779|SAP_BOVIN Proactivator polypeptide precursor [Contains: Saposin A (Protein
A); Saposin B (Sphingolipid activator protein 1) (SAP-1)
(Cerebroside sulfate activator) (CSAct) (Dispersin)
(Sulfatide/GM1 activator); Saposin C
(Co-beta-glucosidase) (A1 activator) (Glucosylceramidase
activator) (Sphingolipid activator protein 2) (SAP-2);
Saposin D (Protein C) (Component C)]
Length = 525
Score = 68.2 bits (165), Expect = 3e-11
Identities = 48/184 (26%), Positives = 98/184 (53%), Gaps = 6/184 (3%)
Frame = +1
Query: 208 IECELCEHVMDEVIKVVTSNHSEEVVSNFIKLKICPRL-SVIKNECVIIVDTYLPVIFSI 384
I CE+CE V+ EV K++ +N +EE + + + K+C +L + + +C +VDTY I SI
Sbjct: 314 IYCEVCEFVVKEVAKLIDNNRTEEEILHALD-KVCSKLPTSLAEQCQEVVDTYGRSILSI 372
Query: 385 ARSTLNSKVLCTELKLCTL--LSKKKISFLMNRSGNETLKCEICRFAILEV-RTLLTDPG 555
+ +++C+ L LC+ L + + + G CE+C+ + + R L +
Sbjct: 373 LLDEASPELVCSMLHLCSSRGLPAATVRVMPRKDGG---FCEVCKKLVGYLDRNLEKNST 429
Query: 556 FQKIFENFLINEICPHFKTK-QQQCTNDINQYYSLVVNIIVEFLNPS-LCSELGLCNSTI 729
++I + + C + ++QC + +Y +++ I+VE ++PS +C ++G C +
Sbjct: 430 KEQILA--ALEKGCSFLPDQYRKQCDQFVTEYEPVLIEILVEVMDPSFVCLKIGACPAAH 487
Query: 730 KQVI 741
K ++
Sbjct: 488 KPLL 491
Score = 48.9 bits (115), Expect = 2e-05
Identities = 38/160 (23%), Positives = 70/160 (43%), Gaps = 13/160 (8%)
Frame = +1
Query: 10 ISDPATETEIKNFLKNDFCYIFGSDKIQCQQIVDEYFNLILNFILAEVDVDKICKFFGLC 189
I + TE EI + L + S QCQ++VD Y IL+ +L E + +C LC
Sbjct: 330 IDNNRTEEEILHALDKVCSKLPTSLAEQCQEVVDTYGRSILSILLDEASPELVCSMLHLC 389
Query: 190 NA------------KLSGIECELCEHVMDEVIKVVTSNHSEEVVSNFIKLKICPRL-SVI 330
++ + G CE+C+ ++ + + + N ++E + ++ K C L
Sbjct: 390 SSRGLPAATVRVMPRKDGGFCEVCKKLVGYLDRNLEKNSTKEQILAALE-KGCSFLPDQY 448
Query: 331 KNECVIIVDTYLPVIFSIARSTLNSKVLCTELKLCTLLSK 450
+ +C V Y PV+ I ++ +C ++ C K
Sbjct: 449 RKQCDQFVTEYEPVLIEILVEVMDPSFVCLKIGACPAAHK 488
Score = 40.4 bits (93), Expect = 0.006
Identities = 44/226 (19%), Positives = 86/226 (38%), Gaps = 43/226 (19%)
Frame = +1
Query: 199 LSGIECELCEHVMDEVIKVVTSNHSEEVVSNFIKLKICPRLSV--IKNECVIIVDTYLPV 372
+ + C++C+ V+ ++ N +E+ + +++ + C L + C IVD+YLPV
Sbjct: 58 VKSLPCDICKDVITAAGNLLKDNATEQEILMYLE-RTCDWLPKPNMSASCKEIVDSYLPV 116
Query: 373 IFSIARSTLNSK-VLCTELKLCTLLSK-----------------------------KKIS 462
I + + ++ +C+ L LC L K I
Sbjct: 117 ILDMIKGQMSHPGEVCSALNLCESLQKHLAELNHQKQLESNQIPELDMAEVVAPFMANIP 176
Query: 463 FLMNRSGNETLK----------CEICRFAILEVR-TLLTDPGFQKIFENFLINEICPHFK 609
FL+ K C+ C + +V+ L T+ F + + E
Sbjct: 177 FLLYPQDGSHSKPQPKKANGNVCQDCIQLVTDVQEALRTNSTFVEALVDHAKEECDRLGP 236
Query: 610 TKQQQCTNDINQYYSLVVNIIVEFLNPSLCSELGLCNSTIKQVIMR 747
C N INQY + + +++ +C G C+ +K++ M+
Sbjct: 237 GMSDMCKNYINQYSEVAIQMVMHMQPKEICVLAGFCDE-VKEMPMK 281
>sp|P42211|ASPRX_ORYSA Aspartic proteinase precursor
Length = 496
Score = 47.4 bits (111), Expect = 5e-05
Identities = 27/104 (25%), Positives = 50/104 (48%), Gaps = 18/104 (17%)
Frame = +1
Query: 91 QCQQIVDEYFNLILNFILAEVDVDKICKFFGLC-----NAKLSGIE-------------C 216
+C+++V EY +ILN ++A+ D K+C GLC + +GIE C
Sbjct: 311 ECKEVVSEYGEMILNLLIAQTDPQKVCSQVGLCMFDGKRSVSNGIESVVDKENLGSDAMC 370
Query: 217 ELCEHVMDEVIKVVTSNHSEEVVSNFIKLKICPRLSVIKNECVI 348
+CE + + + N ++E++ N+ ++C RL E +
Sbjct: 371 SVCEMAVVWIENQLRENKTKELILNYAN-QLCERLPSPNGESTV 413
Score = 35.8 bits (81), Expect = 0.16
Identities = 20/112 (17%), Positives = 50/112 (44%), Gaps = 5/112 (4%)
Frame = +1
Query: 325 VIKNECVIIVDTYLPVIFSIARSTLNSKVLCTELKLCTLLSKKKI-----SFLMNRSGNE 489
+I EC +V Y +I ++ + + + +C+++ LC K+ + S + +
Sbjct: 307 IISTECKEVVSEYGEMILNLLIAQTDPQKVCSQVGLCMFDGKRSVSNGIESVVDKENLGS 366
Query: 490 TLKCEICRFAILEVRTLLTDPGFQKIFENFLINEICPHFKTKQQQCTNDINQ 645
C +C A++ + L + +++ N+ N++C + + T +Q
Sbjct: 367 DAMCSVCEMAVVWIENQLRENKTKELILNY-ANQLCERLPSPNGESTVSCHQ 417
>sp|P10960|SAP_RAT Sulfated glycoprotein 1 precursor (SGP-1) (Prosaposin)
Length = 554
Score = 46.6 bits (109), Expect = 9e-05
Identities = 50/224 (22%), Positives = 97/224 (43%), Gaps = 44/224 (19%)
Frame = +1
Query: 208 IECELCEHVMDEVIKVVTSNHSEEVVSNFIKLKICPRL--SVIKNECVIIVDTYLPVIFS 381
+ C++C+ V+ E ++ N +EE + ++++ K C + S + C +VD+YLPVI
Sbjct: 61 LPCDICKTVVTEAGNLLKDNATEEEILHYLE-KTCAWIHDSSLSASCKEVVDSYLPVILD 119
Query: 382 IARSTL-NSKVLCTELKLCTLLSK------------KKI----------SFLMN------ 474
+ + + N +C+ L LC L + KI F+ N
Sbjct: 120 MIKGEMSNPGEVCSALNLCQSLQEYLAEQNQRQLESNKIPEVDLARVVAPFMSNIPLLLY 179
Query: 475 ----------RSGNETLKCEICRFAILEVRTLL-TDPGF-QKIFENFLINEICPHFKTKQ 618
NE + C+ C + +++T + T+ F Q + ++ + E C
Sbjct: 180 PQDRPRSQPQPKANEDV-CQDCMKLVTDIQTAVRTNSSFVQGLVDH--VKEDCDRLGPGV 236
Query: 619 QQ-CTNDINQYYSLVVNIIVEFLNPSLCSELGLCNSTIKQVIMR 747
C N ++QY + V +++ +C +G C+ +K+V MR
Sbjct: 237 SDICKNYVDQYSEVAVQMMMHMQPKEICVMVGFCDE-VKRVPMR 279
Score = 45.1 bits (105), Expect = 3e-04
Identities = 41/210 (19%), Positives = 90/210 (42%), Gaps = 32/210 (15%)
Frame = +1
Query: 208 IECELCEHVMDEVIKVVTSNHSEEVVSNFIKLKICPRLSV-IKNECVIIVDTYLPVIFSI 384
I C++C+ VM ++ +++ +N +EE++ + K C L +C ++ T+ P + +
Sbjct: 312 IFCQVCQLVMRKLSELIINNATEELLIKGLS-KACSLLPAPASTKCQEVLVTFGPSLLDV 370
Query: 385 ARSTLNSKVLCTELKLCTL------LSKKKISFLMNRSGNETLK---------------- 498
+N LC + LC+ ++ + +++ E
Sbjct: 371 LMHEVNPNFLCGVISLCSANPNLVGTLEQPAAAIVSALPKEPAPPKQPEEPKQSALRAHV 430
Query: 499 --------CEICRFAILEVRTLLTDPGFQKIFENFLINEICPHFKTKQQQCTNDINQYYS 654
CE+C+ ++ + L ++ L Q+QC + +Y
Sbjct: 431 PPQKNGGFCEVCKKLVIYLEHNLEKNSTKEEILAALEKGCSFLPDPYQKQCDEFVAEYEP 490
Query: 655 LVVNIIVEFLNPS-LCSELGLCNSTIKQVI 741
L++ I+VE ++PS +CS++G+C S K ++
Sbjct: 491 LLLEILVEVMDPSFVCSKIGVCPSAYKLLL 520
Score = 38.5 bits (88), Expect = 0.025
Identities = 26/93 (27%), Positives = 47/93 (50%), Gaps = 2/93 (2%)
Frame = +1
Query: 10 ISDPATETEIKNFLKNDFCYIFGSD-KIQCQQIVDEYFNLILNFILAEV-DVDKICKFFG 183
+ D ATE EI ++L+ +I S C+++VD Y +IL+ I E+ + ++C
Sbjct: 77 LKDNATEEEILHYLEKTCAWIHDSSLSASCKEVVDSYLPVILDMIKGEMSNPGEVCSALN 136
Query: 184 LCNAKLSGIECELCEHVMDEVIKVVTSNHSEEV 282
LC + L E++ ++ + + SN EV
Sbjct: 137 LCQS--------LQEYLAEQNQRQLESNKIPEV 161
Score = 38.1 bits (87), Expect = 0.032
Identities = 31/131 (23%), Positives = 62/131 (47%), Gaps = 6/131 (4%)
Frame = +1
Query: 403 SKVLCTELKLCTLLSKKKI--SFLMNRSGNETLKCEICRFAILEVRTLLTDPGFQKIFEN 576
S V+C ++K K + ++ ++L C+IC+ + E LL D ++ +
Sbjct: 29 SAVVCRDVKTAVDCRAVKHCQQMVWSKPTAKSLPCDICKTVVTEAGNLLKDNATEEEILH 88
Query: 577 FLINEICP--HFKTKQQQCTNDINQYYSLVVNIIV-EFLNP-SLCSELGLCNSTIKQVIM 744
+L + C H + C ++ Y +++++I E NP +CS L LC S +++ +
Sbjct: 89 YL-EKTCAWIHDSSLSASCKEVVDSYLPVILDMIKGEMSNPGEVCSALNLCQS-LQEYLA 146
Query: 745 RQPLVQINRSK 777
Q Q+ +K
Sbjct: 147 EQNQRQLESNK 157
Score = 30.0 bits (66), Expect = 8.8
Identities = 15/56 (26%), Positives = 28/56 (50%)
Frame = +1
Query: 22 ATETEIKNFLKNDFCYIFGSDKIQCQQIVDEYFNLILNFILAEVDVDKICKFFGLC 189
+T+ EI L+ ++ + QC + V EY L+L ++ +D +C G+C
Sbjct: 457 STKEEILAALEKGCSFLPDPYQKQCDEFVAEYEPLLLEILVEVMDPSFVCSKIGVC 512
>sp|P20097|SAP_CAVPO Saposin C (CO-beta-glucosidase) (Glucosylceramidase activator)
(Sphingolipid activator protein 2) (SAP-2)
Length = 81
Score = 45.1 bits (105), Expect = 3e-04
Identities = 22/77 (28%), Positives = 47/77 (61%), Gaps = 1/77 (1%)
Frame = +1
Query: 208 IECELCEHVMDEVIKVVTSNHSEEVVSNFIKLKICPRLSVIKNE-CVIIVDTYLPVIFSI 384
+ C+ CE+V+ +V++++ +N +EE + + + +C L +E C +VDTY I ++
Sbjct: 3 VTCKACEYVVKKVMELIDNNRTEEKIIHALD-SVCALLPESVSEVCQEVVDTYGDSIVAL 61
Query: 385 ARSTLNSKVLCTELKLC 435
++ +++C+EL LC
Sbjct: 62 LLQEMSPELVCSELGLC 78
Score = 35.4 bits (80), Expect = 0.21
Identities = 19/80 (23%), Positives = 43/80 (53%), Gaps = 1/80 (1%)
Frame = +1
Query: 487 ETLKCEICRFAILEVRTLLTDPGFQKIFENFLINEICPHFKTKQQQCTNDINQYYSLVVN 666
E++ C+ C + + +V L+ + ++ + L + ++ + C ++ Y +V
Sbjct: 1 ESVTCKACEYVVKKVMELIDNNRTEEKIIHALDSVCALLPESVSEVCQEVVDTYGDSIVA 60
Query: 667 IIVEFLNPSL-CSELGLCNS 723
++++ ++P L CSELGLC S
Sbjct: 61 LLLQEMSPELVCSELGLCMS 80
Score = 34.3 bits (77), Expect = 0.46
Identities = 19/62 (30%), Positives = 32/62 (51%)
Frame = +1
Query: 4 EYISDPATETEIKNFLKNDFCYIFGSDKIQCQQIVDEYFNLILNFILAEVDVDKICKFFG 183
E I + TE +I + L + + S CQ++VD Y + I+ +L E+ + +C G
Sbjct: 17 ELIDNNRTEEKIIHALDSVCALLPESVSEVCQEVVDTYGDSIVALLLQEMSPELVCSELG 76
Query: 184 LC 189
LC
Sbjct: 77 LC 78
>sp|P50405|PSPB_MOUSE Pulmonary surfactant-associated protein B precursor (SP-B)
(Pulmonary surfactant-associated proteolipid SPL(Phe))
Length = 377
Score = 43.5 bits (101), Expect = 8e-04
Identities = 24/96 (25%), Positives = 48/96 (50%), Gaps = 7/96 (7%)
Frame = +1
Query: 169 CKFFGLCNAKLSGIE-----CELCEHVMDEVIKVVTSNHSEEVVSNFIKLK--ICPRLSV 327
C+ G C ++ G C+ CE ++ + K+ + +E + F++ + I P L +
Sbjct: 48 CRALGHCLQEVWGHAGANDLCQECEDIVHLLTKMTKEDAFQEAIRKFLEQECDILP-LKL 106
Query: 328 IKNECVIIVDTYLPVIFSIARSTLNSKVLCTELKLC 435
+ C ++D YLP++ +S +N K +C + LC
Sbjct: 107 LVPRCRQVLDVYLPLVIDYFQSQINPKAICNHVGLC 142
Score = 35.4 bits (80), Expect = 0.21
Identities = 15/57 (26%), Positives = 28/57 (49%)
Frame = +1
Query: 64 CYIFGSDKIQCQQIVDEYFNLILNFILAEVDVDKICKFFGLCNAKLSGIECELCEHV 234
C F D+ +C+Q V+++ +L + D C+ G+C A S ++C H+
Sbjct: 321 CLRFWLDRQKCEQFVEQHMPQLLALVPRSQDAHITCQALGVCEAPASPLQCFQTPHL 377
Score = 33.5 bits (75), Expect = 0.79
Identities = 16/60 (26%), Positives = 34/60 (56%), Gaps = 2/60 (3%)
Frame = +1
Query: 16 DPATETEIKNFLKNDFCYIFGSDKI--QCQQIVDEYFNLILNFILAEVDVDKICKFFGLC 189
+ A + I+ FL+ + C I + +C+Q++D Y L++++ ++++ IC GLC
Sbjct: 84 EDAFQEAIRKFLEQE-CDILPLKLLVPRCRQVLDVYLPLVIDYFQSQINPKAICNHVGLC 142
>sp|Q61207|SAP_MOUSE Sulfated glycoprotein 1 precursor (SGP-1) (Prosaposin)
Length = 557
Score = 42.7 bits (99), Expect = 0.001
Identities = 23/84 (27%), Positives = 47/84 (55%), Gaps = 3/84 (3%)
Frame = +1
Query: 208 IECELCEHVMDEVIKVVTSNHSEEVVSNFIKLKICPRL--SVIKNECVIIVDTYLPVIFS 381
+ C++C+ V+ E ++ N ++E + ++++ K C + S + C +VD+YLPVI
Sbjct: 61 LPCDICKTVVTEAGNLLKDNATQEEILHYLE-KTCEWIHDSSLSASCKEVVDSYLPVILD 119
Query: 382 IARSTL-NSKVLCTELKLCTLLSK 450
+ + + N +C+ L LC L +
Sbjct: 120 MIKGEMSNPGEVCSALNLCQSLQE 143
Score = 41.2 bits (95), Expect = 0.004
Identities = 33/131 (25%), Positives = 62/131 (47%), Gaps = 6/131 (4%)
Frame = +1
Query: 403 SKVLCTELKLCTLLSKKKI--SFLMNRSGNETLKCEICRFAILEVRTLLTDPGFQKIFEN 576
S VLC ++K K + ++ ++L C+IC+ + E LL D Q+ +
Sbjct: 29 SAVLCRDVKTAVDCGAVKHCQQMVWSKPTAKSLPCDICKTVVTEAGNLLKDNATQEEILH 88
Query: 577 FLINEICP--HFKTKQQQCTNDINQYYSLVVNIIV-EFLNP-SLCSELGLCNSTIKQVIM 744
+L + C H + C ++ Y +++++I E NP +CS L LC S +++ +
Sbjct: 89 YL-EKTCEWIHDSSLSASCKEVVDSYLPVILDMIKGEMSNPGEVCSALNLCQS-LQEYLA 146
Query: 745 RQPLVQINRSK 777
Q Q+ +K
Sbjct: 147 EQNQKQLESNK 157
Score = 39.7 bits (91), Expect = 0.011
Identities = 52/247 (21%), Positives = 100/247 (40%), Gaps = 1/247 (0%)
Frame = +1
Query: 4 EYISDPATETEIKNFLKNDFCYIFGSDKIQCQQIVDEYFNLILNFILAEVDVDKICKFFG 183
E I + ATE + L N + + +CQ++V + +L+ + EV+ +C G
Sbjct: 329 ELIVNNATEELLVKGLSNACALLPDPARTKCQEVVGTFGPSLLDIFIHEVNPSSLCGVIG 388
Query: 184 LCNAKLSGIECELCEHVMDEVIKVVTSNHSEEVVSNFIKLKICPRLSVIKNECVIIVDTY 363
LC A+ +E + +VS +K P+ + + +
Sbjct: 389 LCAARPELVE--------------ALEQPAPAIVSALLKEPTPPKQPAQPKQSAL--PAH 432
Query: 364 LPVIFSIARSTLNSKVLCTELKLCTLLSKKKISFLMNRSGNETLKCEICRFAILEVRTLL 543
+P + C ++C KK + +L + + K EI A+ + + L
Sbjct: 433 VP--------PQKNGGFC---EVC----KKLVLYLEHNLEKNSTKEEILA-ALEKGCSFL 476
Query: 544 TDPGFQKIFENFLINEICPHFKTKQQQCTNDINQYYSLVVNIIVEFLNPS-LCSELGLCN 720
DP +QK QC + + +Y L++ I+VE ++P +CS++G+C
Sbjct: 477 PDP-YQK-------------------QCDDFVAEYEPLLLEILVEVMDPGFVCSKIGVCP 516
Query: 721 STIKQVI 741
S K ++
Sbjct: 517 SAYKLLL 523
Score = 37.0 bits (84), Expect = 0.072
Identities = 26/93 (27%), Positives = 47/93 (50%), Gaps = 2/93 (2%)
Frame = +1
Query: 10 ISDPATETEIKNFLKNDFCYIFGSD-KIQCQQIVDEYFNLILNFILAEV-DVDKICKFFG 183
+ D AT+ EI ++L+ +I S C+++VD Y +IL+ I E+ + ++C
Sbjct: 77 LKDNATQEEILHYLEKTCEWIHDSSLSASCKEVVDSYLPVILDMIKGEMSNPGEVCSALN 136
Query: 184 LCNAKLSGIECELCEHVMDEVIKVVTSNHSEEV 282
LC + L E++ ++ K + SN EV
Sbjct: 137 LCQS--------LQEYLAEQNQKQLESNKIPEV 161
>sp|P17129|PSPB_CANFA Pulmonary surfactant-associated protein B precursor (SP-B) (6 kDa
protein) (Pulmonary surfactant-associated proteolipid
SPL(Phe)) (Pulmonary surfactant protein 18) (SP 18)
Length = 363
Score = 40.8 bits (94), Expect = 0.005
Identities = 24/97 (24%), Positives = 50/97 (51%), Gaps = 8/97 (8%)
Frame = +1
Query: 169 CKFFGLC------NAKLSGIECELCEHVMDEVIKVVTSNHSEEVVSNFIKLK--ICPRLS 324
C+ G C NA+ + C+ C+ ++ + K+ +++V F++ + + P L
Sbjct: 42 CRALGHCLQEVWGNARADDL-CQECQDIVRILTKMTKEAIFQDMVRKFLEHECDVLP-LK 99
Query: 325 VIKNECVIIVDTYLPVIFSIARSTLNSKVLCTELKLC 435
++ +C ++ TY PV+ +S +N K++C L LC
Sbjct: 100 LLTPQCHHMLGTYFPVVVDYFQSQINPKIICKHLGLC 136
Score = 34.7 bits (78), Expect = 0.36
Identities = 16/55 (29%), Positives = 32/55 (58%), Gaps = 2/55 (3%)
Frame = +1
Query: 91 QCQQIVDEYFNLILNFILAEVDVDKICKFFGLCNAKL--SGIECELCEHVMDEVI 249
QC ++ YF +++++ ++++ ICK GLC L E EL + ++D++I
Sbjct: 104 QCHHMLGTYFPVVVDYFQSQINPKIICKHLGLCKPGLPEPEQESELSDPLLDKLI 158
Score = 33.9 bits (76), Expect = 0.61
Identities = 21/76 (27%), Positives = 37/76 (48%), Gaps = 3/76 (3%)
Frame = +1
Query: 499 CEICRFAILEVRTLLTDPGFQKIFENFLINE--ICPHFKTKQQQCTNDINQYYSLVVNII 672
C+ C+ + + + + FQ + FL +E + P K QC + + Y+ +VV+
Sbjct: 62 CQECQDIVRILTKMTKEAIFQDMVRKFLEHECDVLP-LKLLTPQCHHMLGTYFPVVVDYF 120
Query: 673 VEFLNPS-LCSELGLC 717
+NP +C LGLC
Sbjct: 121 QSQINPKIICKHLGLC 136
>sp|P22355|PSPB_RAT Pulmonary surfactant-associated protein B precursor (SP-B)
(Pulmonary surfactant-associated proteolipid SPL(Phe))
Length = 376
Score = 40.0 bits (92), Expect = 0.008
Identities = 22/98 (22%), Positives = 48/98 (48%), Gaps = 7/98 (7%)
Frame = +1
Query: 169 CKFFGLCNAKLSGIE-----CELCEHVMDEVIKVVTSNHSEEVVSNFIKLK--ICPRLSV 327
C+ G C ++ G C+ CE ++ + K+ + ++ + F++ + I P L +
Sbjct: 47 CRALGHCLQEVWGHAGANDLCQECEDIVHLLTKMTKEDAFQDTIRKFLEQECDILP-LKL 105
Query: 328 IKNECVIIVDTYLPVIFSIARSTLNSKVLCTELKLCTL 441
+ C ++D YLP++ + + K +C+ + LC L
Sbjct: 106 LVPRCRQVLDVYLPLVIDYFQGQIKPKAICSHVGLCPL 143
Score = 35.8 bits (81), Expect = 0.16
Identities = 15/57 (26%), Positives = 28/57 (49%)
Frame = +1
Query: 64 CYIFGSDKIQCQQIVDEYFNLILNFILAEVDVDKICKFFGLCNAKLSGIECELCEHV 234
C F D+ +C+Q V+++ +L + D C+ G+C A S ++C H+
Sbjct: 320 CLRFWLDRQKCEQFVEQHMPQLLALVPRSQDAHTSCQALGVCEAPASPLQCFQTPHL 376
Score = 32.3 bits (72), Expect = 1.8
Identities = 16/60 (26%), Positives = 32/60 (53%), Gaps = 2/60 (3%)
Frame = +1
Query: 16 DPATETEIKNFLKNDFCYIFGSDKI--QCQQIVDEYFNLILNFILAEVDVDKICKFFGLC 189
+ A + I+ FL+ + C I + +C+Q++D Y L++++ ++ IC GLC
Sbjct: 83 EDAFQDTIRKFLEQE-CDILPLKLLVPRCRQVLDVYLPLVIDYFQGQIKPKAICSHVGLC 141
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 91,594,363
Number of Sequences: 369166
Number of extensions: 1751162
Number of successful extensions: 5010
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4741
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4998
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 8646143400
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)