Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_006_H19
(881 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q6F0Z2|LEPA_MESFL GTP-binding protein lepA 33 1.4
sp|Q9VTK2|POMT1_DROME Protein O-mannosyltransferase 1 (Doli... 31 5.2
sp|P06173|RPOB_SALTY DNA-directed RNA polymerase beta chain... 31 5.2
sp|Q20065|P4HA2_CAEEL Prolyl 4-hydroxylase alpha-2 subunit ... 30 6.8
sp|O94508|CWF11_SCHPO Cell cycle control protein cwf11 30 6.8
sp|Q56P11|RPOC2_LACSA DNA-directed RNA polymerase beta'' ch... 30 6.8
sp|Q8D1U8|KUP_WIGBR Low affinity potassium transport system... 30 8.9
sp||Q62600_2 [Segment 2 of 3] Nitric-oxide synthase, endoth... 30 8.9
>sp|Q6F0Z2|LEPA_MESFL GTP-binding protein lepA
Length = 600
Score = 32.7 bits (73), Expect = 1.4
Identities = 13/37 (35%), Positives = 24/37 (64%)
Frame = +2
Query: 326 EDRVLTEMNVERKRALEPSKNDIALWYKLKNDQENPF 436
++++L M++ER+R + N + L+YK K+ QE F
Sbjct: 39 QEQLLDSMDIERERGITIKLNSVQLYYKAKDGQEYTF 75
>sp|Q9VTK2|POMT1_DROME Protein O-mannosyltransferase 1
(Dolichyl-phosphate-mannose--protein mannosyltransferase
1) (dPOMT1) (Rotated abdomen protein)
Length = 886
Score = 30.8 bits (68), Expect = 5.2
Identities = 18/73 (24%), Positives = 30/73 (41%)
Frame = -1
Query: 428 SPDHFSICTTRQYHSWTVLMLAFSQHSFQSTHDPHLLYK*LESLNSEQHLFLYH*HYFSW 249
S F++ SWT+ +LAF ++ PH+++ L HY +
Sbjct: 163 SSQPFTVNLRIDLFSWTLFLLAFGTRFYKLATPPHIVFDEL--------------HYGKY 208
Query: 248 VSKYQMLLLQFDQ 210
+S Y + FDQ
Sbjct: 209 ISMYMRNIFFFDQ 221
>sp|P06173|RPOB_SALTY DNA-directed RNA polymerase beta chain (RNAP beta subunit)
(Transcriptase beta chain) (RNA polymerase beta subunit)
Length = 1342
Score = 30.8 bits (68), Expect = 5.2
Identities = 33/142 (23%), Positives = 66/142 (46%), Gaps = 2/142 (1%)
Frame = +2
Query: 278 KDVAQNLATRATCTINEDRVLTEMNVER-KRALEPSKNDIALWYKLKNDQENPFKSDLFI 454
KD + + + T+ + +V T VE+ KRALE + + K +++ ++ LF
Sbjct: 914 KDSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLFS 973
Query: 455 RV-LYLIGSWAGGEGXXXXXXXXIHDQFLTEEQIRENQLKEQRVNVERLKHELASGDIQY 631
R+ L+ S E + LT+E+ ++NQL++ + LKHE + +
Sbjct: 974 RIRAVLVSSGVEAEKLDKLPRDRWLELGLTDEE-KQNQLEQLAEQYDELKHEF---EKKL 1029
Query: 632 QSKSGTVTPVELIYQSIPNLVK 697
++K +T + + + +VK
Sbjct: 1030 EAKRRKITQGDDLAPGVLKIVK 1051
>sp|Q20065|P4HA2_CAEEL Prolyl 4-hydroxylase alpha-2 subunit precursor (4-PH alpha-2)
(Procollagen-proline,2-oxoglutarate-4-dioxygenase
alpha-2 subunit)
Length = 539
Score = 30.4 bits (67), Expect = 6.8
Identities = 18/70 (25%), Positives = 31/70 (44%), Gaps = 5/70 (7%)
Frame = +2
Query: 368 ALEPSKNDIALWYKLKNDQENPFKS-----DLFIRVLYLIGSWAGGEGXXXXXXXXIHDQ 532
A+ PSKND WY L+ D E ++ + + V ++ W +G +
Sbjct: 464 AVFPSKNDALFWYNLRRDGEGDLRTRHAACPVLLGVKWVSNKWIHEKGQEFTRPCGL--- 520
Query: 533 FLTEEQIREN 562
EE+++EN
Sbjct: 521 ---EEEVQEN 527
>sp|O94508|CWF11_SCHPO Cell cycle control protein cwf11
Length = 1284
Score = 30.4 bits (67), Expect = 6.8
Identities = 19/53 (35%), Positives = 27/53 (50%)
Frame = +2
Query: 149 SNYSTLKSSNSEPTAHYLLHPDQIGVKAFDIWRPRRSSANGIEKDVAQNLATR 307
+N S L S ++ YLLH KAFD ++ +R S +EK NL +R
Sbjct: 147 TNISILNSLDNLDKVKYLLHDSSSLTKAFDSYKEKRPSI--VEKFPLHNLLSR 197
>sp|Q56P11|RPOC2_LACSA DNA-directed RNA polymerase beta'' chain (PEP) (Plastid-encoded RNA
polymerase beta'' subunit) (RNA polymerase beta''
subunit)
Length = 1386
Score = 30.4 bits (67), Expect = 6.8
Identities = 51/230 (22%), Positives = 86/230 (37%), Gaps = 26/230 (11%)
Frame = +2
Query: 32 RQRYMSCPSINNQEVTVKF--------RQKLPRIHRQSFIDEFSCA-HSN--YSTLKSSN 178
+ RY++ PS+ N +V KF QK RI S ++ +C HSN Y + N
Sbjct: 510 KPRYIANPSVTNNQVRHKFFSSYFSGKNQKGDRIPDCSELNRMTCTDHSNLRYPAILDGN 569
Query: 179 SEPTAHYLLHPDQIGVKAFDIWRPRRSSANGIEKDVAQNLATRATCT---------INED 331
S+ A + I +++ + ++GI ++ +N R I +
Sbjct: 570 SDLLAKRRRNRFIIPLESIQEGENQLIPSSGISMEIPRNGILRRNSILAYFDDPRYIRKS 629
Query: 332 RVLTEMNVERKRALEPSKNDI------ALWYKLKNDQENPFKSDLFIRVLYLIGSWAGGE 493
LT+ ++ +N I W K + + NPF FI V I S +
Sbjct: 630 SGLTKYETRELNSIVNEENLIEYRGVKVFWPKYQK-EVNPF---FFIPVEVHILSESSSI 685
Query: 494 GXXXXXXXXIHDQFLTEEQIRENQLKEQRVNVERLKHELASGDIQYQSKS 643
Q + R L + E++K + SGDI + K+
Sbjct: 686 MVRHNSIIGADTQITFNRRSRVGGLVRVKKKAEKMKLIIFSGDIHFPGKT 735
>sp|Q8D1U8|KUP_WIGBR Low affinity potassium transport system protein kup (Kup system
potassium uptake protein)
Length = 625
Score = 30.0 bits (66), Expect = 8.9
Identities = 17/51 (33%), Positives = 29/51 (56%), Gaps = 1/51 (1%)
Frame = -2
Query: 346 FSQHTILIYCTSSSSR*ILSNIFFYTISTTSPGS-PNIKCFYSNLIRMQKV 197
F T I + SS+ I+S+IF+ T G PNIK FY++ ++ +++
Sbjct: 290 FFTFTFAIIISIISSQNIISSIFYLTRQAVRLGYLPNIKIFYTSEVKSRRI 340
>sp||Q62600_2 [Segment 2 of 3] Nitric-oxide synthase, endothelial (EC-NOS) (NOS
type III) (NOSIII) (Endothelial NOS) (eNOS)
(Constitutive NOS) (cNOS)
Length = 390
Score = 30.0 bits (66), Expect = 8.9
Identities = 15/58 (25%), Positives = 30/58 (51%)
Frame = +2
Query: 8 YSPTKSYKRQRYMSCPSINNQEVTVKFRQKLPRIHRQSFIDEFSCAHSNYSTLKSSNS 181
+SP +S+KRQRY ++ Q +++ +L +HR+ + N + KS+ +
Sbjct: 252 FSPKRSWKRQRY----RLSTQAESLQLLPRLTHVHRRKMFQATILSVENLQSSKSTRA 305
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 93,477,404
Number of Sequences: 369166
Number of extensions: 1754816
Number of successful extensions: 3972
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 3844
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3969
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 8790245790
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)