Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_006_F12
(551 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P70191|TRAF5_MOUSE TNF receptor-associated factor 5 56 7e-08
sp|O00463|TRAF5_HUMAN TNF receptor-associated factor 5 (RIN... 55 1e-07
sp|Q13114|TRAF3_HUMAN TNF receptor-associated factor 3 (CD4... 47 4e-05
sp|Q60803|TRAF3_MOUSE TNF receptor-associated factor 3 (CD4... 47 4e-05
sp|Q9BUZ4|TRAF4_HUMAN TNF receptor-associated factor 4 (Cys... 39 0.011
sp|Q61382|TRAF4_MOUSE TNF receptor-associated factor 4 (Cys... 38 0.019
sp|P70196|TRAF6_MOUSE TNF receptor-associated factor 6 37 0.042
sp|P11467|DG17_DICDI Protein DG17 36 0.055
sp|Q922B6|TRAF7_MOUSE E3 ubiquitin protein ligase TRAF7 (TN... 35 0.094
sp|Q9Y4K3|TRAF6_HUMAN TNF receptor-associated factor 6 (Int... 35 0.094
>sp|P70191|TRAF5_MOUSE TNF receptor-associated factor 5
Length = 558
Score = 55.8 bits (133), Expect = 7e-08
Identities = 31/102 (30%), Positives = 51/102 (50%)
Frame = +3
Query: 63 VKCPEEKCSFECRLIDMDEHLKVCDNKTEKCPFFGYGCTHKIKSKNMNSHMLREAQDHNN 242
V CP +C ++EHL VC + CPF YGCT K K N+ H QDH
Sbjct: 193 VSCPN-RCVQTIPRARVNEHLTVCPEAEQDCPFKHYGCTVKGKRGNLLEHERAALQDHML 251
Query: 243 MLIDLLGNLKNEVIDLREKEKNNHDEIEKLKEKGRVQDEKLK 368
++++ L+ + DL + + +I++L E + +++LK
Sbjct: 252 LVLEKNYQLEQRISDLYQSLEQKESKIQQLAETVKKFEKELK 293
>sp|O00463|TRAF5_HUMAN TNF receptor-associated factor 5 (RING finger protein 84)
Length = 557
Score = 54.7 bits (130), Expect = 1e-07
Identities = 29/106 (27%), Positives = 52/106 (49%), Gaps = 6/106 (5%)
Frame = +3
Query: 69 CPEEK--CSFECRLI----DMDEHLKVCDNKTEKCPFFGYGCTHKIKSKNMNSHMLREAQ 230
CPE C C I ++DEHL VC + CPF YGC K +N+ H +
Sbjct: 188 CPEYPVFCPNNCAKIILKTEVDEHLAVCPEAEQDCPFKHYGCAVTDKRRNLQQHEHSALR 247
Query: 231 DHNNMLIDLLGNLKNEVIDLREKEKNNHDEIEKLKEKGRVQDEKLK 368
+H ++++ L+ ++ DL + + +I++L E + +++ K
Sbjct: 248 EHMRLVLEKNVQLEEQISDLHKSLEQKESKIQQLAETIKKLEKEFK 293
>sp|Q13114|TRAF3_HUMAN TNF receptor-associated factor 3 (CD40 receptor associated factor
1) (CRAF1) (CD40 binding protein) (CD40BP) (LMP1
associated protein) (LAP1) (CAP-1)
Length = 568
Score = 46.6 bits (109), Expect = 4e-05
Identities = 31/117 (26%), Positives = 53/117 (45%), Gaps = 4/117 (3%)
Frame = +3
Query: 45 DISNLTVKCPEEKCSFECRL-IDMDEHLKVCDNKTEKCPFFGYGCTHKIKSKNMNSHMLR 221
D + V CP KCS + L ++ HL C N C F YGC + ++ + +H
Sbjct: 196 DCPCVVVSCPH-KCSVQTLLRSELSAHLSECVNAPSTCSFKRYGCVFQGTNQQIKAHEAS 254
Query: 222 EAQDHNNMLIDLLGNLKNEVIDLREKEKNNHDEIEKLKEK---GRVQDEKLKAMYEN 383
A H N+L + +L+ +V L+ + + I+ L + ++ E+ K M N
Sbjct: 255 SAVQHVNLLKEWSNSLEKKVSLLQNESVEKNKSIQSLHNQICSFEIEIERQKEMLRN 311
>sp|Q60803|TRAF3_MOUSE TNF receptor-associated factor 3 (CD40 receptor associated factor
1) (CRAF1) (TRAFAMN)
Length = 567
Score = 46.6 bits (109), Expect = 4e-05
Identities = 31/117 (26%), Positives = 53/117 (45%), Gaps = 4/117 (3%)
Frame = +3
Query: 45 DISNLTVKCPEEKCSFECRL-IDMDEHLKVCDNKTEKCPFFGYGCTHKIKSKNMNSHMLR 221
D + V CP KCS + L ++ HL C N C F YGC + ++ + +H
Sbjct: 195 DCPCVVVSCPH-KCSVQTLLRSELSAHLSECVNAPSTCSFKRYGCVFQGTNQQIKAHEAS 253
Query: 222 EAQDHNNMLIDLLGNLKNEVIDLREKEKNNHDEIEKLKEK---GRVQDEKLKAMYEN 383
A H N+L + +L+ +V L+ + + I+ L + ++ E+ K M N
Sbjct: 254 SAVQHVNLLKEWSNSLEKKVSLLQNESVEKNKSIQSLHNQICSFEIEIERQKEMLRN 310
>sp|Q9BUZ4|TRAF4_HUMAN TNF receptor-associated factor 4 (Cysteine-rich domain associated
with RING and Traf domains protein 1) (Malignant 62)
(RING finger protein 83)
Length = 470
Score = 38.5 bits (88), Expect = 0.011
Identities = 26/100 (26%), Positives = 42/100 (42%), Gaps = 1/100 (1%)
Frame = +3
Query: 3 ENISQTVINHRLNEDISNLTVKCPEEKCSFECRLIDMDEHLK-VCDNKTEKCPFFGYGCT 179
E + T+ +H+ L V CP + D+ HLK C+ CPF GC
Sbjct: 201 EFVFDTIQSHQYQ--CPRLPVACPNQCGVGTVAREDLPGHLKDSCNTALVLCPFKDSGCK 258
Query: 180 HKIKSKNMNSHMLREAQDHNNMLIDLLGNLKNEVIDLREK 299
H+ M H+ + H M+ L+ + E+ +LR +
Sbjct: 259 HRCPKLAMARHVEESVKPHLAMMCALVSRQRQELQELRRE 298
>sp|Q61382|TRAF4_MOUSE TNF receptor-associated factor 4 (Cysteine-rich motif associated to
RING and Traf domains protein 1)
Length = 470
Score = 37.7 bits (86), Expect = 0.019
Identities = 26/100 (26%), Positives = 41/100 (41%), Gaps = 1/100 (1%)
Frame = +3
Query: 3 ENISQTVINHRLNEDISNLTVKCPEEKCSFECRLIDMDEHLK-VCDNKTEKCPFFGYGCT 179
E + T+ +H+ L V CP + D+ HLK C CPF GC
Sbjct: 201 EFVYDTIQSHQYQ--CPRLPVPCPNQCGVGTVAREDLPTHLKDSCRTAFVLCPFKESGCK 258
Query: 180 HKIKSKNMNSHMLREAQDHNNMLIDLLGNLKNEVIDLREK 299
H+ M H+ + H M+ L+ + E+ +LR +
Sbjct: 259 HRCPKLAMGRHVEESVKPHLAMMCALVSRQRQELQELRRE 298
>sp|P70196|TRAF6_MOUSE TNF receptor-associated factor 6
Length = 530
Score = 36.6 bits (83), Expect = 0.042
Identities = 21/92 (22%), Positives = 40/92 (43%)
Frame = +3
Query: 45 DISNLTVKCPEEKCSFECRLIDMDEHLKVCDNKTEKCPFFGYGCTHKIKSKNMNSHMLRE 224
+I +LTVKCP + C + L +++H C+ CP C + +N+H++ +
Sbjct: 126 EILSLTVKCPNKGCLQKMELRHLEDHQVHCEFALVNCP----QCQRPFQKCQVNTHIIED 181
Query: 225 AQDHNNMLIDLLGNLKNEVIDLREKEKNNHDE 320
+ N + + +EK HD+
Sbjct: 182 CPRRQVSCV-------NCAVSMAYEEKEIHDQ 206
Score = 28.9 bits (63), Expect = 8.8
Identities = 17/95 (17%), Positives = 40/95 (42%), Gaps = 9/95 (9%)
Frame = +3
Query: 132 CDNKTEKCPFFGYGCTHKIKSKNMNSHMLREAQDHNNMLIDLLGNLK---------NEVI 284
C C F +GC K++ ++ H+ Q H +L + N+ +
Sbjct: 235 CPTAPIPCTFSVFGCHQKMQRNHLARHLQENTQLHMRLLAQAVHNVNLALRPCDAASPSR 294
Query: 285 DLREKEKNNHDEIEKLKEKGRVQDEKLKAMYENLQ 389
R ++ N + I++L+ + QD +++ + ++
Sbjct: 295 GCRPEDPNYEETIKQLESRLVRQDHQIRELTAKME 329
>sp|P11467|DG17_DICDI Protein DG17
Length = 458
Score = 36.2 bits (82), Expect = 0.055
Identities = 23/83 (27%), Positives = 40/83 (48%), Gaps = 1/83 (1%)
Frame = +3
Query: 84 CSFECRLIDMDEHLKV-CDNKTEKCPFFGYGCTHKIKSKNMNSHMLREAQDHNNMLIDLL 260
CS + + +H++ C N C +F GC ++K + +H+ E +H N + L+
Sbjct: 209 CSVKIERKSIIDHIENDCCNTQIPCKYFEQGCKVEMKRSELQNHL--ERVNHQNYMGILI 266
Query: 261 GNLKNEVIDLREKEKNNHDEIEK 329
L N+V + K HDE+ K
Sbjct: 267 EKLTNQV----GQSKKTHDELLK 285
>sp|Q922B6|TRAF7_MOUSE E3 ubiquitin protein ligase TRAF7 (TNF receptor-associated factor
7)
Length = 594
Score = 35.4 bits (80), Expect = 0.094
Identities = 32/123 (26%), Positives = 51/123 (41%), Gaps = 15/123 (12%)
Frame = +3
Query: 63 VKCPEEKCSFECRLIDMDEHLKVCDNKTEKCPFFGYGCTHKIKSKNMNSHM--------- 215
V+CP ++++ HLK C++ KCP YGCT +H+
Sbjct: 155 VRCPNNPSCPPLLKMNLEAHLKECEHI--KCPHSKYGCTFIGNQDTYETHLETCRFEGLK 212
Query: 216 --LREAQDHNNMLIDLLGNLKNEVIDLRE---KEKNNHDEIEK-LKEKGRVQDEKLKAMY 377
L++ D + + L E+ LR K D++EK L+ K V DE +
Sbjct: 213 EFLQQTDDRFHEMHVALAQKDQEIAFLRSMLGKLSEKIDQLEKSLELKFDVLDENQSKLS 272
Query: 378 ENL 386
E+L
Sbjct: 273 EDL 275
>sp|Q9Y4K3|TRAF6_HUMAN TNF receptor-associated factor 6 (Interleukin 1 signal transducer)
(RING finger protein 85)
Length = 522
Score = 35.4 bits (80), Expect = 0.094
Identities = 17/60 (28%), Positives = 30/60 (50%)
Frame = +3
Query: 45 DISNLTVKCPEEKCSFECRLIDMDEHLKVCDNKTEKCPFFGYGCTHKIKSKNMNSHMLRE 224
+I +L VKCP E C + L +++H C+ CP C + ++N H+L++
Sbjct: 126 EILSLMVKCPNEGCLHKMELRHLEDHQAHCEFALMDCP----QCQRPFQKFHINIHILKD 181
Score = 29.3 bits (64), Expect = 6.7
Identities = 12/46 (26%), Positives = 20/46 (43%)
Frame = +3
Query: 132 CDNKTEKCPFFGYGCTHKIKSKNMNSHMLREAQDHNNMLIDLLGNL 269
C C F +GC K++ ++ H+ Q H ML + +L
Sbjct: 235 CPTAPIPCTFSTFGCHEKMQRNHLARHLQENTQSHMRMLAQAVHSL 280
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 51,590,934
Number of Sequences: 369166
Number of extensions: 857971
Number of successful extensions: 3522
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3261
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3500
length of database: 68,354,980
effective HSP length: 104
effective length of database: 49,142,540
effective search space used: 3882260660
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)