Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_006_E11
(881 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P28648|CD63_RAT CD63 antigen (AD1 antigen) 43 0.001
sp|P41731|CD63_MOUSE CD63 antigen 40 0.011
sp|Q9UKR8|TNS16_HUMAN Tetraspanin-16 (Tspan-16) (Transmembr... 40 0.011
sp|O43657|TSN6_HUMAN Tetraspanin-6 (Tspan-6) (Transmembrane... 38 0.033
sp|Q80WR1|TSN18_MOUSE Tetraspanin-18 (Tspan-18) 38 0.043
sp|O60635|TSN1_HUMAN Tetraspanin-1 (Tspan-1) (Tetraspan NET... 38 0.043
sp|O70401|TSN6_MOUSE Tetraspanin-6 (Tspan-6) (Transmembrane... 37 0.056
sp|P08962|CD63_HUMAN CD63 antigen (Melanoma-associated anti... 37 0.073
sp|O70352|CD82_RAT CD82 antigen (Metastasis suppressor homo... 37 0.073
sp|Q9QY33|TSN3_MOUSE Tetraspanin-3 (Tspan-3) (Transmembrane... 37 0.095
>sp|P28648|CD63_RAT CD63 antigen (AD1 antigen)
Length = 238
Score = 43.1 bits (100), Expect = 0.001
Identities = 37/156 (23%), Positives = 59/156 (37%)
Frame = +3
Query: 339 KK*ISKNLGKSLTNVLEKDFNGILNTNATLFTKIFTAIMFELKCCGVNNVTDFDSVRNWK 518
K SK+ K + N L N AT+ K+ E KCCG +N TD++ +
Sbjct: 112 KSEFSKSFQKQMQNYLTD------NKTATILDKL----QKENKCCGASNYTDWERIPGM- 160
Query: 519 STSSSGTKQIYPVGCCKLKEKYQYDPNKKFEYNDAKKNFENDNCPFDNDKSTININGCIP 698
K P CC N C D +STI+ GC+
Sbjct: 161 ------AKDRVPDSCCI--------------------NI-TVGCGNDFKESTIHTQGCVE 193
Query: 699 SLIDLIEKNMXXXXXXXXXXXXFEIMCIVFACCVMR 806
++ + KN+ E++ I+F+CC+++
Sbjct: 194 TIAAWLRKNVLLVAGAALGIAFVEVLGIIFSCCLVK 229
>sp|P41731|CD63_MOUSE CD63 antigen
Length = 238
Score = 39.7 bits (91), Expect = 0.011
Identities = 30/132 (22%), Positives = 51/132 (38%)
Frame = +3
Query: 411 NTNATLFTKIFTAIMFELKCCGVNNVTDFDSVRNWKSTSSSGTKQIYPVGCCKLKEKYQY 590
N AT+ K+ E CCG +N TD++++ K P CC
Sbjct: 130 NKTATILDKL----QKENNCCGASNYTDWENIPGM-------AKDRVPDSCCI------- 171
Query: 591 DPNKKFEYNDAKKNFENDNCPFDNDKSTININGCIPSLIDLIEKNMXXXXXXXXXXXXFE 770
N C D +STI+ GC+ ++ + KN+ E
Sbjct: 172 -------------NI-TVGCGNDFKESTIHTQGCVETIAIWLRKNILLVAAAALGIAFVE 217
Query: 771 IMCIVFACCVMR 806
++ I+F+CC+++
Sbjct: 218 VLGIIFSCCLVK 229
>sp|Q9UKR8|TNS16_HUMAN Tetraspanin-16 (Tspan-16) (Transmembrane 4 superfamily member 16)
(Tetraspanin TM4-B)
Length = 245
Score = 39.7 bits (91), Expect = 0.011
Identities = 19/40 (47%), Positives = 22/40 (55%)
Frame = +3
Query: 450 IMFELKCCGVNNVTDFDSVRNWKSTSSSGTKQIYPVGCCK 569
+M +LKCCGVNN TDF S+ T YP CCK
Sbjct: 147 VMEKLKCCGVNNYTDFSG-----SSFEMTTGHTYPRSCCK 181
>sp|O43657|TSN6_HUMAN Tetraspanin-6 (Tspan-6) (Transmembrane 4 superfamily member 6)
(T245 protein) (Tetraspanin TM4-D) (A15 homolog)
Length = 245
Score = 38.1 bits (87), Expect = 0.033
Identities = 29/115 (25%), Positives = 43/115 (37%)
Frame = +3
Query: 462 LKCCGVNNVTDFDSVRNWKSTSSSGTKQIYPVGCCKLKEKYQYDPNKKFEYNDAKKNFEN 641
L CCGV TD+ R+W T+ K +P CCKL++
Sbjct: 151 LHCCGV---TDY---RDWTDTNYYSEKG-FPKSCCKLED--------------------- 182
Query: 642 DNCPFDNDKSTININGCIPSLIDLIEKNMXXXXXXXXXXXXFEIMCIVFACCVMR 806
C D +N GC ++ +IE M F+++ I A C+ R
Sbjct: 183 --CTPQRDADKVNNEGCFIKVMTIIESEMGVVAGISFGVACFQLIGIFLAYCLSR 235
>sp|Q80WR1|TSN18_MOUSE Tetraspanin-18 (Tspan-18)
Length = 248
Score = 37.7 bits (86), Expect = 0.043
Identities = 32/144 (22%), Positives = 60/144 (41%)
Frame = +3
Query: 375 TNVLEKDFNGILNTNATLFTKIFTAIMFELKCCGVNNVTDFDSVRNWKSTSSSGTKQIYP 554
T L K + G +T+ +F+ + ++M CCGVN DF ++ + T+++ P
Sbjct: 118 TKELTKHYQGDNDTD--VFSATWNSVMITFGCCGVNGPEDFKLASVFRLLTLD-TEEV-P 173
Query: 555 VGCCKLKEKYQYDPNKKFEYNDAKKNFENDNCPFDNDKSTININGCIPSLIDLIEKNMXX 734
CC+ +P + +++ + PF IN GC +++ E +
Sbjct: 174 KACCR------REPQTRDGVVLSREECQLGRNPF------INKQGCYTVILNTFETYVYL 221
Query: 735 XXXXXXXXXXFEIMCIVFACCVMR 806
E+ +VFA C+ R
Sbjct: 222 AGAFAIGVLAIELFLMVFAMCLFR 245
Score = 34.7 bits (78), Expect = 0.36
Identities = 20/78 (25%), Positives = 34/78 (43%)
Frame = +1
Query: 43 GAVILGVGIWVKVDKSNLFEVIRNIKLDDTTASFNNTNSTILDVVAIVLIIXXXXXXXXX 222
GA +LGVGIWV VD + E++ + +L A +++
Sbjct: 24 GACLLGVGIWVLVDPTGFREIV--------------ATNPLLTTGAYIVLAMGGLLFLLG 69
Query: 223 XXXXXXAIKENKCLLITY 276
A++EN+CLL+ +
Sbjct: 70 FLGCCGAVRENRCLLLFF 87
>sp|O60635|TSN1_HUMAN Tetraspanin-1 (Tspan-1) (Tetraspan NET-1) (Tetraspanin TM4-C)
Length = 241
Score = 37.7 bits (86), Expect = 0.043
Identities = 27/99 (27%), Positives = 37/99 (37%)
Frame = +3
Query: 429 FTKIFTAIMFELKCCGVNNVTDFDSVRNWKSTSSSGTKQIYPVGCCKLKEKYQYDPNKKF 608
FT+++ M LKCCG N TDF+ +K S+ +P CC
Sbjct: 136 FTQVWNTTMKGLKCCGFTNYTDFEDSPYFKENSA------FPPFCC-------------- 175
Query: 609 EYNDAKKNFENDNCPFDNDKSTININGCIPSLIDLIEKN 725
ND N N+ C + GC L+ I N
Sbjct: 176 --NDNVTNTANETCTKQKAHDQ-KVEGCFNQLLYDIRTN 211
Score = 30.0 bits (66), Expect = 8.9
Identities = 19/78 (24%), Positives = 33/78 (42%)
Frame = +1
Query: 43 GAVILGVGIWVKVDKSNLFEVIRNIKLDDTTASFNNTNSTILDVVAIVLIIXXXXXXXXX 222
GA +L VGIWV +D ++ ++ L + F N ++ +V +
Sbjct: 22 GAALLAVGIWVSIDGASFLKIFG--PLSSSAMQFVNVGYFLIAAGVVVFAL--------G 71
Query: 223 XXXXXXAIKENKCLLITY 276
A E+KC L+T+
Sbjct: 72 FLGCYGAKTESKCALVTF 89
>sp|O70401|TSN6_MOUSE Tetraspanin-6 (Tspan-6) (Transmembrane 4 superfamily member 6)
Length = 245
Score = 37.4 bits (85), Expect = 0.056
Identities = 34/151 (22%), Positives = 50/151 (33%)
Frame = +3
Query: 354 KNLGKSLTNVLEKDFNGILNTNATLFTKIFTAIMFELKCCGVNNVTDFDSVRNWKSTSSS 533
KN KS K++N + + KI + L CCGV N D+ + T
Sbjct: 119 KNSFKSNYENALKEYNSTGDYRSEAVDKIQST----LHCCGVTNYGDWKGTNYYSETG-- 172
Query: 534 GTKQIYPVGCCKLKEKYQYDPNKKFEYNDAKKNFENDNCPFDNDKSTININGCIPSLIDL 713
+P CCKL + C D +N GC ++
Sbjct: 173 -----FPKSCCKL-----------------------EGCYPQRDADKVNEEGCFIKVMTT 204
Query: 714 IEKNMXXXXXXXXXXXXFEIMCIVFACCVMR 806
IE M F+++ I A C+ R
Sbjct: 205 IESEMGVVAGISFGVACFQLIGIFLAYCLSR 235
>sp|P08962|CD63_HUMAN CD63 antigen (Melanoma-associated antigen ME491)
(Lysosome-associated membrane glycoprotein 3) (LAMP-3)
(Ocular melanoma-associated antigen) (OMA81H)
(Granulophysin) (Tetraspanin-30) (Tspan-30)
Length = 238
Score = 37.0 bits (84), Expect = 0.073
Identities = 26/123 (21%), Positives = 45/123 (36%)
Frame = +3
Query: 438 IFTAIMFELKCCGVNNVTDFDSVRNWKSTSSSGTKQIYPVGCCKLKEKYQYDPNKKFEYN 617
I + + KCCG N TD++ + S +K P CC
Sbjct: 135 ILDRMQADFKCCGAANYTDWEKI-------PSMSKNRVPDSCCINVTV------------ 175
Query: 618 DAKKNFENDNCPFDNDKSTININGCIPSLIDLIEKNMXXXXXXXXXXXXFEIMCIVFACC 797
C + ++ I+ GC+ + + KN+ E++ IVFACC
Sbjct: 176 ---------GCGINFNEKAIHKEGCVEKIGGWLRKNVLVVAAAALGIAFVEVLGIVFACC 226
Query: 798 VMR 806
+++
Sbjct: 227 LVK 229
>sp|O70352|CD82_RAT CD82 antigen (Metastasis suppressor homolog)
Length = 266
Score = 37.0 bits (84), Expect = 0.073
Identities = 25/80 (31%), Positives = 35/80 (43%)
Frame = +1
Query: 43 GAVILGVGIWVKVDKSNLFEVIRNIKLDDTTASFNNTNSTILDVVAIVLIIXXXXXXXXX 222
GAVILG G+W+ DKS+ V++ T+S+ L V A V I
Sbjct: 24 GAVILGFGVWILADKSSFISVLQ-------------TSSSSLQVGAYVFIGVGAITMLMG 70
Query: 223 XXXXXXAIKENKCLLITYSI 282
A+ E +CLL Y +
Sbjct: 71 FLGCIGAVNEVRCLLGLYFV 90
>sp|Q9QY33|TSN3_MOUSE Tetraspanin-3 (Tspan-3) (Transmembrane 4 superfamily member 8)
(Tetraspanin TM4-A) (OSP-associated protein 1) (OAP-1)
Length = 253
Score = 36.6 bits (83), Expect = 0.095
Identities = 19/74 (25%), Positives = 39/74 (52%)
Frame = +3
Query: 348 ISKNLGKSLTNVLEKDFNGILNTNATLFTKIFTAIMFELKCCGVNNVTDFDSVRNWKSTS 527
+ + +S+ V K +NG TN+ ++ + +L CCG++N +D+++ +K T
Sbjct: 111 VENEVDRSIQKVY-KTYNG---TNSDAASRAIDYVQRQLHCCGIHNYSDWENTDWFKETK 166
Query: 528 SSGTKQIYPVGCCK 569
+ Q P+ CC+
Sbjct: 167 N----QSVPLSCCR 176
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 89,838,057
Number of Sequences: 369166
Number of extensions: 1734569
Number of successful extensions: 4579
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4366
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4564
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 8790245790
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)