Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_006_E07
(864 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9P289|MST4_HUMAN Serine/threonine-protein kinase MST4 (... 107 6e-23
sp|Q99JT2|MST4_MOUSE Serine/threonine-protein kinase MST4 (... 106 7e-23
sp|P23561|STE11_YEAST Serine/threonine-protein kinase STE11 104 3e-22
sp|P41892|CDC7_SCHPO Cell division control protein 7 104 4e-22
sp|P32490|MKK1_YEAST MAP kinase kinase MKK1/SSP32 103 6e-22
sp|Q9Y6E0|STK24_HUMAN Serine/threonine-protein kinase 24 (S... 100 4e-21
sp|P35465|PAK1_RAT Serine/threonine-protein kinase PAK 1 (p... 100 7e-21
sp|Q99KH8|STK24_MOUSE Serine/threonine-protein kinase 24 100 7e-21
sp|Q9Y2U5|M3K2_HUMAN Mitogen-activated protein kinase kinas... 100 7e-21
sp|Q12469|SKM1_YEAST Serine/threonine-protein kinase SKM1 (... 100 9e-21
>sp|Q9P289|MST4_HUMAN Serine/threonine-protein kinase MST4 (STE20-like kinase MST4)
(MST-4) (Mammalian STE20-like protein kinase 4)
(Serine/threonine-protein kinase MASK) (Mst3 and
SOK1-related kinase)
Length = 416
Score = 107 bits (266), Expect = 6e-23
Identities = 78/256 (30%), Positives = 120/256 (46%), Gaps = 1/256 (0%)
Frame = +3
Query: 12 EPFDTGMYSEIYKARDQVKNQSIVLKRY-ISDDGDLIEKYRKLINGLIDFQRKGGQERFV 188
E G + E++K D Q + +K + + D IE ++ I L
Sbjct: 28 ERIGKGSFGEVFKGIDNRTQQVVAIKIIDLEEAEDEIEDIQQEITVLSQCD----SSYVT 83
Query: 189 KLYSFYPDPCSFAIGMEWMEGGSMDNYVKNFWPFTDKQLIRWTHQILGGLDFLHDNNVIF 368
K Y Y I ME++ GGS + ++ PF + Q+ +IL GLD+LH I
Sbjct: 84 KYYGSYLKGSKLWIIMEYLGGGSALDLLRA-GPFDEFQIATMLKEILKGLDYLHSEKKIH 142
Query: 369 RDLKPSNVLLDENNNTKLCAFSLSIYLMSNHEMELKRSSSQSKVGVYSYMAPEVLNSEPY 548
RD+K +NVLL E + KL F ++ L + ++KR++ VG +MAPEV+ Y
Sbjct: 143 RDIKAANVLLSEQGDVKLADFGVAGQLT---DTQIKRNTF---VGTPFWMAPEVIQQSAY 196
Query: 549 DNAADVWSFAAVLVNLITGKNPCPDKDPIDAAFHVARKLEMEYTLPEDTNQVLKDIITNC 728
D+ AD+WS + L G+ P D P+ F + + TL D + K+ I C
Sbjct: 197 DSKADIWSLGITAIELAKGEPPNSDMHPMRVLFLIPK--NNPPTLVGDFTKSFKEFIDAC 254
Query: 729 AQGDPNKRPKIKELIE 776
DP+ RP KEL++
Sbjct: 255 LNKDPSFRPTAKELLK 270
>sp|Q99JT2|MST4_MOUSE Serine/threonine-protein kinase MST4 (STE20-like kinase MST4)
(MST-4) (Mammalian STE20-like protein kinase 4)
Length = 416
Score = 106 bits (265), Expect = 7e-23
Identities = 78/256 (30%), Positives = 120/256 (46%), Gaps = 1/256 (0%)
Frame = +3
Query: 12 EPFDTGMYSEIYKARDQVKNQSIVLKRY-ISDDGDLIEKYRKLINGLIDFQRKGGQERFV 188
E G + E++K D Q + +K + + D IE ++ I L
Sbjct: 28 ERIGKGSFGEVFKGIDNRTQQVVAIKIIDLEEAEDEIEDIQQEITVLSQCD----SSYVT 83
Query: 189 KLYSFYPDPCSFAIGMEWMEGGSMDNYVKNFWPFTDKQLIRWTHQILGGLDFLHDNNVIF 368
K Y Y I ME++ GGS + ++ PF + Q+ +IL GLD+LH I
Sbjct: 84 KYYGSYLKGSKLWIIMEYLGGGSALDLLRA-GPFDEFQIATMLKEILKGLDYLHSEKKIH 142
Query: 369 RDLKPSNVLLDENNNTKLCAFSLSIYLMSNHEMELKRSSSQSKVGVYSYMAPEVLNSEPY 548
RD+K +NVLL E + KL F ++ L + ++KR++ VG +MAPEV+ Y
Sbjct: 143 RDIKAANVLLSEQGDVKLADFGVAGQLT---DTQIKRNTF---VGTPFWMAPEVIQQSAY 196
Query: 549 DNAADVWSFAAVLVNLITGKNPCPDKDPIDAAFHVARKLEMEYTLPEDTNQVLKDIITNC 728
D+ AD+WS + L G+ P D P+ F + + TL D + K+ I C
Sbjct: 197 DSKADIWSLGITAIELAKGEPPNSDMHPMRVLFLIPK--NNPPTLIGDFTKSFKEFIDAC 254
Query: 729 AQGDPNKRPKIKELIE 776
DP+ RP KEL++
Sbjct: 255 LNKDPSFRPTAKELLK 270
>sp|P23561|STE11_YEAST Serine/threonine-protein kinase STE11
Length = 738
Score = 104 bits (260), Expect = 3e-22
Identities = 63/220 (28%), Positives = 112/220 (50%), Gaps = 3/220 (1%)
Frame = +3
Query: 126 YRKLINGL---IDFQRKGGQERFVKLYSFYPDPCSFAIGMEWMEGGSMDNYVKNFWPFTD 296
+RK+++ L ++ ++ E V Y + + I +E++ GGS+ + + N+ PF +
Sbjct: 515 HRKMVDALQHEMNLLKELHHENIVTYYGASQEGGNLNIFLEYVPGGSVSSMLNNYGPFEE 574
Query: 297 KQLIRWTHQILGGLDFLHDNNVIFRDLKPSNVLLDENNNTKLCAFSLSIYLMSNHEMELK 476
+ +T QIL G+ +LH N+I RD+K +N+L+D K+ F +S L ++ + K
Sbjct: 575 SLITNFTRQILIGVAYLHKKNIIHRDIKGANILIDIKGCVKITDFGISKKLSPLNKKQNK 634
Query: 477 RSSSQSKVGVYSYMAPEVLNSEPYDNAADVWSFAAVLVNLITGKNPCPDKDPIDAAFHVA 656
R+S Q V +M+PEV+ AD+WS V++ + TGK+P PD + A F +
Sbjct: 635 RASLQGSV---FWMSPEVVKQTATTAKADIWSTGCVVIEMFTGKHPFPDFSQMQAIFKIG 691
Query: 657 RKLEMEYTLPEDTNQVLKDIITNCAQGDPNKRPKIKELIE 776
E +P K+ + + D RP EL++
Sbjct: 692 TNTTPE--IPSWATSEGKNFLRKAFELDYQYRPSALELLQ 729
>sp|P41892|CDC7_SCHPO Cell division control protein 7
Length = 1062
Score = 104 bits (259), Expect = 4e-22
Identities = 66/250 (26%), Positives = 116/250 (46%)
Frame = +3
Query: 27 GMYSEIYKARDQVKNQSIVLKRYISDDGDLIEKYRKLINGLIDFQRKGGQERFVKLYSFY 206
G + +Y+ + +++ +K+ +++ +I ID + VK Y
Sbjct: 18 GAFGAVYRGLNIKNGETVAVKKVKLSK--MLKSDLSVIKMEIDLLKNLDHPNIVKYRGSY 75
Query: 207 PDPCSFAIGMEWMEGGSMDNYVKNFWPFTDKQLIRWTHQILGGLDFLHDNNVIFRDLKPS 386
S I +E+ E GS+ + KNF + + +T Q+L GL +LH+ VI RD+K +
Sbjct: 76 QTNDSLCIILEYCENGSLRSICKNFGKIPENLVALYTFQVLQGLLYLHNQGVIHRDIKGA 135
Query: 387 NVLLDENNNTKLCAFSLSIYLMSNHEMELKRSSSQSKVGVYSYMAPEVLNSEPYDNAADV 566
N+L ++ KL F ++ ++ S VG +MAPEV+ A+D+
Sbjct: 136 NILTTKDGTIKLADFGVA--------TKINALEDHSVVGSPYWMAPEVIELVGATTASDI 187
Query: 567 WSFAAVLVNLITGKNPCPDKDPIDAAFHVARKLEMEYTLPEDTNQVLKDIITNCAQGDPN 746
WS ++ L+ G P D DP A F + + + LP + + K + C Q DPN
Sbjct: 188 WSVGCTVIELLDGNPPYYDLDPTSALFRMVK--DEHPPLPSNISSAAKSFLMQCFQKDPN 245
Query: 747 KRPKIKELIE 776
R K ++L++
Sbjct: 246 LRIKTRKLLK 255
>sp|P32490|MKK1_YEAST MAP kinase kinase MKK1/SSP32
Length = 508
Score = 103 bits (257), Expect = 6e-22
Identities = 73/253 (28%), Positives = 113/253 (44%), Gaps = 15/253 (5%)
Frame = +3
Query: 60 QVKNQSIVLKRYISDDGDLIEKYRKLINGLIDFQRKGGQERFVKLYSFYPDP--CSFAIG 233
++KN S + + + + +Y+K I + F R E V+ Y + D S I
Sbjct: 239 KLKNGSKIFALKVINTLNTDPEYQKQIFRELQFNRSFQSEYIVRYYGMFTDDENSSIYIA 298
Query: 234 MEWMEGGSMDNYVKNFWP----FTDKQLIRWTHQILGGLDFLHDNNVIFRDLKPSNVLLD 401
ME+M G S+D KN ++K L + +L GL +LH+ VI RD+KP N+LL+
Sbjct: 299 MEYMGGRSLDAIYKNLLERGGRISEKVLGKIAEAVLRGLSYLHEKKVIHRDIKPQNILLN 358
Query: 402 ENNNTKLCAFSLSIYLMSNHEMELKRSSSQSKVGVYSYMAPEVLNSEPYDNAADVWSFAA 581
EN KLC F +S E S + + G YMAPE + +PY +DVWS
Sbjct: 359 ENGQVKLCDFGVS--------GEAVNSLATTFTGTSFYMAPERIQGQPYSVTSDVWSLGL 410
Query: 582 VLVNLITGKNPCPDKD------PIDAAFHVARKLEMEYTLPEDT---NQVLKDIITNCAQ 734
++ + GK PC + P + + PE + K I C +
Sbjct: 411 TILEVANGKFPCSSEKMAANIAPFELLMWILTFTPELKDEPESNIIWSPSFKSFIDYCLK 470
Query: 735 GDPNKRPKIKELI 773
D +RP +++I
Sbjct: 471 KDSRERPSPRQMI 483
>sp|Q9Y6E0|STK24_HUMAN Serine/threonine-protein kinase 24 (STE20-like kinase MST3) (MST-3)
(Mammalian STE20-like protein kinase 3)
Length = 443
Score = 100 bits (250), Expect = 4e-21
Identities = 75/260 (28%), Positives = 121/260 (46%), Gaps = 5/260 (1%)
Frame = +3
Query: 12 EPFDTGMYSEIYKARDQVKNQSIVLKRY-ISDDGDLIEKYRKLINGLIDFQRKGGQERFV 188
E G + E++K D + + +K + + D IE ++ I L
Sbjct: 40 EKIGKGSFGEVFKGIDNRTQKVVAIKIIDLEEAEDEIEDIQQEITVLSQCD----SPYVT 95
Query: 189 KLYSFYPDPCSFAIGMEWMEGGSMDNYVKNFWPFTDKQLIRWTHQILGGLDFLHDNNVIF 368
K Y Y I ME++ GGS + ++ P + Q+ +IL GLD+LH I
Sbjct: 96 KYYGSYLKDTKLWIIMEYLGGGSALDLLEP-GPLDETQIATILREILKGLDYLHSEKKIH 154
Query: 369 RDLKPSNVLLDENNNTKLCAFSLSIYLMSNHEMELKRSSSQSKVGVYSYMAPEVLNSEPY 548
RD+K +NVLL E+ KL F ++ L + ++KR++ VG +MAPEV+ Y
Sbjct: 155 RDIKAANVLLSEHGEVKLADFGVAGQLT---DTQIKRNTF---VGTPFWMAPEVIKQSAY 208
Query: 549 DNAADVWSFAAVLVNLITGKNPCPDKDPIDAAFHVARK----LEMEYTLPEDTNQVLKDI 716
D+ AD+WS + L G+ P + P+ F + + LE Y+ P LK+
Sbjct: 209 DSKADIWSLGITAIELARGEPPHSELHPMKVLFLIPKNNPPTLEGNYSKP------LKEF 262
Query: 717 ITNCAQGDPNKRPKIKELIE 776
+ C +P+ RP KEL++
Sbjct: 263 VEACLNKEPSFRPTAKELLK 282
>sp|P35465|PAK1_RAT Serine/threonine-protein kinase PAK 1 (p21-activated kinase 1)
(PAK-1) (P68-PAK) (Alpha-PAK) (Protein kinase MUK2)
Length = 544
Score = 100 bits (248), Expect = 7e-21
Identities = 68/261 (26%), Positives = 114/261 (43%)
Frame = +3
Query: 12 EPFDTGMYSEIYKARDQVKNQSIVLKRYISDDGDLIEKYRKLINGLIDFQRKGGQERFVK 191
E G +Y A D Q + +K+ + ++LI I R+ V
Sbjct: 273 EKIGQGASGTVYTAMDVATGQEVAIKQMNLQQ----QPKKELIINEILVMRENKNPNIVN 328
Query: 192 LYSFYPDPCSFAIGMEWMEGGSMDNYVKNFWPFTDKQLIRWTHQILGGLDFLHDNNVIFR 371
Y + ME++ GGS+ + V + Q+ + L L+FLH N VI R
Sbjct: 329 YLDSYLVGDELWVVMEYLAGGSLTDVVTETC-MDEGQIAAVCRECLQALEFLHSNQVIHR 387
Query: 372 DLKPSNVLLDENNNTKLCAFSLSIYLMSNHEMELKRSSSQSKVGVYSYMAPEVLNSEPYD 551
D+K N+LL + + KL F + ++S + VG +MAPEV+ + Y
Sbjct: 388 DIKSDNILLGMDGSVKLTDFGFCAQITP------EQSKRSTMVGTPYWMAPEVVTRKAYG 441
Query: 552 NAADVWSFAAVLVNLITGKNPCPDKDPIDAAFHVARKLEMEYTLPEDTNQVLKDIITNCA 731
D+WS + + +I G+ P +++P+ A + +A E PE + + +D + C
Sbjct: 442 PKVDIWSLGIMAIEMIEGEPPYLNENPLRALYLIATNGTPELQNPEKLSAIFRDFLNRCL 501
Query: 732 QGDPNKRPKIKELIEIDLQKI 794
+ D KR KEL++ KI
Sbjct: 502 EMDVEKRGSAKELLQHQFLKI 522
>sp|Q99KH8|STK24_MOUSE Serine/threonine-protein kinase 24
Length = 431
Score = 100 bits (248), Expect = 7e-21
Identities = 75/260 (28%), Positives = 121/260 (46%), Gaps = 5/260 (1%)
Frame = +3
Query: 12 EPFDTGMYSEIYKARDQVKNQSIVLKRY-ISDDGDLIEKYRKLINGLIDFQRKGGQERFV 188
E G + E++K D + + +K + + D IE ++ I L
Sbjct: 28 EKIGKGSFGEVFKGIDNRTQKVVAIKIIDLEEAEDEIEDIQQEITVLSQCD----SPYVT 83
Query: 189 KLYSFYPDPCSFAIGMEWMEGGSMDNYVKNFWPFTDKQLIRWTHQILGGLDFLHDNNVIF 368
K Y Y I ME++ GGS + ++ P + Q+ +IL GLD+LH I
Sbjct: 84 KYYGSYLKDTKLWIIMEYLGGGSALDLLEP-GPLDEIQIATILREILKGLDYLHSEKKIH 142
Query: 369 RDLKPSNVLLDENNNTKLCAFSLSIYLMSNHEMELKRSSSQSKVGVYSYMAPEVLNSEPY 548
RD+K +NVLL E+ KL F ++ L + ++KR++ VG +MAPEV+ Y
Sbjct: 143 RDIKAANVLLSEHGEVKLADFGVAGQLT---DTQIKRNTF---VGTPFWMAPEVIKQSAY 196
Query: 549 DNAADVWSFAAVLVNLITGKNPCPDKDPIDAAFHVARK----LEMEYTLPEDTNQVLKDI 716
D+ AD+WS + L G+ P + P+ F + + LE Y+ P LK+
Sbjct: 197 DSKADIWSLGITAIELAKGEPPHSELHPMKVLFLIPKNNPPTLEGNYSKP------LKEF 250
Query: 717 ITNCAQGDPNKRPKIKELIE 776
+ C +P+ RP KEL++
Sbjct: 251 VEACLNKEPSFRPTAKELLK 270
>sp|Q9Y2U5|M3K2_HUMAN Mitogen-activated protein kinase kinase kinase 2 (MAPK/ERK kinase
kinase 2) (MEK kinase 2) (MEKK 2)
Length = 618
Score = 100 bits (248), Expect = 7e-21
Identities = 72/254 (28%), Positives = 124/254 (48%), Gaps = 5/254 (1%)
Frame = +3
Query: 27 GMYSEIYKARDQVKNQSIVLKRYISDDGDLIEKYRKLINGL---IDFQRKGGQERFVKLY 197
G + +Y D + + +K+ + D D E ++ +N L I + ER V+ Y
Sbjct: 364 GAFGRVYLCYDVDTGRELAVKQ-VQFDPDSPETSKE-VNALECEIQLLKNFLHERIVQYY 421
Query: 198 SFYPDPC--SFAIGMEWMEGGSMDNYVKNFWPFTDKQLIRWTHQILGGLDFLHDNNVIFR 371
DP + +I ME+M GGS+ + +K + T+ ++T QIL G+ +LH N ++ R
Sbjct: 422 GCLRDPQEKTLSIFMEYMPGGSIKDQLKAYGALTENGTRKYTRQILEGVHYLHSNMILHR 481
Query: 372 DLKPSNVLLDENNNTKLCAFSLSIYLMSNHEMELKRSSSQSKVGVYSYMAPEVLNSEPYD 551
D+K +N+L D N KL F S L + + L + +S G +M+PEV++ + Y
Sbjct: 482 DIKGANILRDSTGNVKLGDFGASKRLQT---ICLSGTGMKSVTGTPYWMSPEVISGQGYG 538
Query: 552 NAADVWSFAAVLVNLITGKNPCPDKDPIDAAFHVARKLEMEYTLPEDTNQVLKDIITNCA 731
AD+WS A +V ++T K P + + + A F +A + LP + +D +
Sbjct: 539 RKADIWSVACTVVEMLTEKPPWAEFEAMAAIFKIATQ-PTNPKLPPHVSDYTRDFLKRIF 597
Query: 732 QGDPNKRPKIKELI 773
+ RP EL+
Sbjct: 598 V-EAKLRPSADELL 610
>sp|Q12469|SKM1_YEAST Serine/threonine-protein kinase SKM1 (Protein kinase 75490 D)
Length = 655
Score = 99.8 bits (247), Expect = 9e-21
Identities = 60/213 (28%), Positives = 102/213 (47%), Gaps = 10/213 (4%)
Frame = +3
Query: 174 QERFVK-LYSFYPDPCSFAIGMEWMEGGSM---------DNYVKNFWPFTDKQLIRWTHQ 323
QE V L ++ D + ME+MEGG + N ++ P + Q+ +
Sbjct: 431 QENIVNFLEAYIIDDEELWVIMEYMEGGCLTDILDAVARSNTGEHSSPLNENQMAYIVKE 490
Query: 324 ILGGLDFLHDNNVIFRDLKPSNVLLDENNNTKLCAFSLSIYLMSNHEMELKRSSSQSKVG 503
GL FLH+ +I RD+K N+LL+ K+ F + E+ KRS + VG
Sbjct: 491 TCQGLKFLHNKKIIHRDIKSDNILLNSQGLVKITDFGFCV------ELTEKRSKRATMVG 544
Query: 504 VYSYMAPEVLNSEPYDNAADVWSFAAVLVNLITGKNPCPDKDPIDAAFHVARKLEMEYTL 683
+MAPE++N + YD DVWS +L+ +I G+ P ++DP+ A + +A +
Sbjct: 545 TPYWMAPEIVNQKGYDEKVDVWSLGIMLIEMIEGEPPYLNEDPLKALYLIANNGSPKLRH 604
Query: 684 PEDTNQVLKDIITNCAQGDPNKRPKIKELIEID 782
PE ++ K + C Q + R +++L+ +
Sbjct: 605 PESVSKQTKQFLDACLQVNVESRASVRKLLTFE 637
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 104,989,209
Number of Sequences: 369166
Number of extensions: 2256067
Number of successful extensions: 9337
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 7220
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 7663
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8582957970
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)