Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_006_D21
(813 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q8VCM8|NCLN_MOUSE Nicalin precursor (Nicastrin-like prot... 171 3e-42
sp|Q5XIA1|NCLN_RAT Nicalin precursor (Nicastrin-like protein) 169 8e-42
sp|Q969V3|NCLN_HUMAN Nicalin precursor (Nicastrin-like prot... 169 1e-41
sp|Q6NZ07|NCLN_BRARE Nicalin-1 precursor (Nicastrin-like pr... 165 2e-40
sp|Q5ZJH2|NCLN_CHICK Nicalin precursor (Nicastrin-like prot... 143 5e-34
sp|O31501|SWRC_BACSU Swarming motility protein swrC 35 0.32
sp|Q5MZM0|Y2310_SYNP6 Hypothetical UPF0182 protein syc2310_c 34 0.54
sp|Q68XE8|SYT_RICTY Threonyl-tRNA synthetase (Threonine--tR... 32 2.1
sp|P22003|BMP5_HUMAN Bone morphogenetic protein 5 precursor... 31 4.6
sp|P54675|PI3K3_DICDI Phosphatidylinositol 3-kinase 3 (PI3-... 31 4.6
>sp|Q8VCM8|NCLN_MOUSE Nicalin precursor (Nicastrin-like protein)
Length = 563
Score = 171 bits (432), Expect = 3e-42
Identities = 96/239 (40%), Positives = 144/239 (60%), Gaps = 10/239 (4%)
Frame = +2
Query: 44 LFVHVSKPPKEGTSGHRLVEALNYTAT--LFNSSVNIVHKRINLNDDELQWEHERFSLRK 217
L +HVSKPP+EGT H + L A + S ++VHK+INL DD L WEHERF++R+
Sbjct: 320 LRLHVSKPpreGTLQHAFLRELETVAAHQFPDVSFSMVHKKINLADDVLAWEHERFAIRR 379
Query: 218 IPAITIS-AEKDRLNNRKSILD--DRISVELLQRNTLSVADSLMRLLYGLEDGHGP---- 376
+PA T+S E R R SI+D R+ + L RNT +A++L R++Y L + P
Sbjct: 380 LPAFTLSHLESHRAGPRSSIMDVRSRVDSKTLTRNTRIIAEALTRVIYNLTEKGTPPDMP 439
Query: 377 -ITEDHWVSEEAINADLALLTRFPRGQQLIDPKHPLVLSWERMFNRYLQNVRTTKIRADP 553
TE V EE I++ + LT PR QL+D + + E +RYL++VR ++AD
Sbjct: 440 VFTEQMQVQEEQIDSVMDWLTNQPRAAQLLDKDGTFLSTLEHFLSRYLKDVRQHHVKADK 499
Query: 554 RDPEFVLYSDAEVIVTAHRVKPALFDFMVACVIISYLGLVYLFIENFSSISRFVLKISL 730
RDPEFV Y + ++ A+RVKPA+FD ++A I +YLG+ Y +++F + + V ++ L
Sbjct: 500 RDPEFVFYDQLKQVMNAYRVKPAIFDLLLALCIGAYLGMAYTAVQHFHVLYKTVQRLLL 558
>sp|Q5XIA1|NCLN_RAT Nicalin precursor (Nicastrin-like protein)
Length = 563
Score = 169 bits (428), Expect = 8e-42
Identities = 95/239 (39%), Positives = 144/239 (60%), Gaps = 10/239 (4%)
Frame = +2
Query: 44 LFVHVSKPPKEGTSGHRLVEALNYTAT--LFNSSVNIVHKRINLNDDELQWEHERFSLRK 217
L +HVSKPP+EGT H + L A + S ++VHK+INL DD L WEHERF++R+
Sbjct: 320 LRLHVSKPpreGTLQHVFLRELEMVAAHQFPDVSFSMVHKKINLADDVLAWEHERFAIRR 379
Query: 218 IPAITIS-AEKDRLNNRKSILD--DRISVELLQRNTLSVADSLMRLLYGLEDGHGP---- 376
+PA T+S E R R SI+D R+ + L RNT +A++L R++Y L + P
Sbjct: 380 LPAFTLSHLENHRAGPRSSIMDVRSRVDSKTLTRNTRIIAEALTRVIYNLTEKGTPPDMP 439
Query: 377 -ITEDHWVSEEAINADLALLTRFPRGQQLIDPKHPLVLSWERMFNRYLQNVRTTKIRADP 553
TE V +E I++ + LT PR QL+D + + E +RYL++VR ++AD
Sbjct: 440 VFTEQMQVQQEQIDSVMDWLTNQPRAAQLLDKDGTFLSTLEHFLSRYLKDVRQHHVKADK 499
Query: 554 RDPEFVLYSDAEVIVTAHRVKPALFDFMVACVIISYLGLVYLFIENFSSISRFVLKISL 730
RDPEFV Y + ++ A+RVKPA+FD ++A I +YLG+ Y +++F + + V ++ L
Sbjct: 500 RDPEFVFYDQLKQVMNAYRVKPAIFDLLLALCIGAYLGMAYTAVQHFHVLYKTVQRLLL 558
>sp|Q969V3|NCLN_HUMAN Nicalin precursor (Nicastrin-like protein)
Length = 563
Score = 169 bits (427), Expect = 1e-41
Identities = 97/255 (38%), Positives = 153/255 (60%), Gaps = 10/255 (3%)
Frame = +2
Query: 8 VCLERLALSDGGLFVHVSKPPKEGTSGHRLVEALNYTATLFNSSV--NIVHKRINLNDDE 181
+CL+ + L +HVSKPP+EGT H + L A V ++VHKRINL +D
Sbjct: 309 LCLDTVGRGSS-LHLHVSKPpreGTLQHAFLRELETVAAHQFPEVRFSMVHKRINLAEDV 367
Query: 182 LQWEHERFSLRKIPAITIS-AEKDRLNNRKSILD--DRISVELLQRNTLSVADSLMRLLY 352
L WEHERF++R++PA T+S E R R SI+D R+ + L RNT +A++L R++Y
Sbjct: 368 LAWEHERFAIRRLPAFTLSHLESHRDGQRSSIMDVRSRVDSKTLTRNTRIIAEALTRVIY 427
Query: 353 GLEDGHGP-----ITEDHWVSEEAINADLALLTRFPRGQQLIDPKHPLVLSWERMFNRYL 517
L + P TE + +E +++ + LT PR QL+D + + E +RYL
Sbjct: 428 NLTEKGTPPDMPVFTEQMQIQQEQLDSVMDWLTNQPRAAQLVDKDSTFLSTLEHHLSRYL 487
Query: 518 QNVRTTKIRADPRDPEFVLYSDAEVIVTAHRVKPALFDFMVACVIISYLGLVYLFIENFS 697
++V+ ++AD RDPEFV Y + ++ A+RVKPA+FD ++A I +YLG+ Y+ +++FS
Sbjct: 488 KDVKQHHVKADKRDPEFVFYDQLKQVMNAYRVKPAVFDLLLAVGIAAYLGMAYVAVQHFS 547
Query: 698 SISRFVLKISLVQEK 742
+ + V ++ LV+ K
Sbjct: 548 LLYKTVQRL-LVKAK 561
>sp|Q6NZ07|NCLN_BRARE Nicalin-1 precursor (Nicastrin-like protein 1)
Length = 572
Score = 165 bits (417), Expect = 2e-40
Identities = 102/270 (37%), Positives = 154/270 (57%), Gaps = 25/270 (9%)
Frame = +2
Query: 8 VCLERLALSDGGLFVHVSKPPKEGTSGHRLVEALNYTATLFNSSVN--IVHKRINLNDDE 181
+CL+ L SD L +HVSKPPKEG+ + L++ L + + +VHK+INL DD
Sbjct: 302 LCLDTLGNSDN-LHLHVSKPPKEGSPQYTLLKELETVVAHQHPDLKFAMVHKKINLADDT 360
Query: 182 LQWEHERFSLRKIPAITIS-AEKDRLNNRKSILDDR-----------------ISVELLQ 307
L WEHERF +R++PA T+S E R R SI+D R + + L
Sbjct: 361 LAWEHERFGIRRLPAFTLSHLESHRSPARHSIMDMRSVSPSLEGAGEATTGPHVDLGKLS 420
Query: 308 RNTLSVADSLMRLLYGLED----GHGPI-TEDHWVSEEAINADLALLTRFPRGQQLIDPK 472
RNT +A++L R++Y L + G I TE V E+ + + + LT PR QL+D
Sbjct: 421 RNTKVIAETLARVIYNLTEKGVTGDLEIFTEQMQVQEDQLASLVDWLTAQPRAAQLLDKD 480
Query: 473 HPLVLSWERMFNRYLQNVRTTKIRADPRDPEFVLYSDAEVIVTAHRVKPALFDFMVACVI 652
++ + E +RYL++V+ +RAD RDPEFV Y + + A+RVKPA+FD ++A I
Sbjct: 481 SSIINTLEHQLSRYLKDVKRHLVRADKRDPEFVFYDQLKQTMNAYRVKPAIFDLLLAVCI 540
Query: 653 ISYLGLVYLFIENFSSISRFVLKISLVQEK 742
SYLG++YL I+NF + F+ +++ + K
Sbjct: 541 ASYLGVLYLAIQNFGLLYGFLRRVTAPRVK 570
>sp|Q5ZJH2|NCLN_CHICK Nicalin precursor (Nicastrin-like protein)
Length = 562
Score = 143 bits (361), Expect = 5e-34
Identities = 90/259 (34%), Positives = 138/259 (53%), Gaps = 18/259 (6%)
Frame = +2
Query: 8 VCLERLALSDGGLFVHVSKPPKEGTSGHRLVEALNYTATLFNSSV--NIVHKRINLNDDE 181
+CL+ L + L +HVSKPPKEGT H + L V ++VHK+INL +D
Sbjct: 308 LCLDTLGRGNS-LHLHVSKPPKEGTLQHAFLRELEMVVASQFPEVKFSMVHKKINLAEDI 366
Query: 182 LQWEHERFSLRKIPAITIS-AEKDRLNNRKSILDDRISVELLQRNTLSVADSLMRLLYGL 358
L WEHERF++R++PA TIS E R + R SI+D R V+ T ++ G
Sbjct: 367 LAWEHERFAIRRLPAFTISHLESHRDSLRNSIMDRRSQVD-----TKALTQEYQDHCGGF 421
Query: 359 EDGHGPITEDHWVSEEAINADL---------------ALLTRFPRGQQLIDPKHPLVLSW 493
++GH + + +ADL L + + QLID + +
Sbjct: 422 DEGH---LQPNREGSTCRSADLHGSDADPGGAARISDGLAEQSAQAAQLIDKDSTFLSTL 478
Query: 494 ERMFNRYLQNVRTTKIRADPRDPEFVLYSDAEVIVTAHRVKPALFDFMVACVIISYLGLV 673
E RYL++V+ ++AD RDPEFV Y + ++ A+RVKPA+FD ++A I +YLG+
Sbjct: 479 EYYMGRYLKDVKQHHVKADKRDPEFVFYDQLKQVMNAYRVKPAIFDLLLAVCIAAYLGVA 538
Query: 674 YLFIENFSSISRFVLKISL 730
Y+ ++NF + R + ++SL
Sbjct: 539 YVAVQNFGLLYRMIQRLSL 557
>sp|O31501|SWRC_BACSU Swarming motility protein swrC
Length = 1052
Score = 34.7 bits (78), Expect = 0.32
Identities = 23/69 (33%), Positives = 31/69 (44%)
Frame = +2
Query: 155 KRINLNDDELQWEHERFSLRKIPAITISAEKDRLNNRKSILDDRISVELLQRNTLSVADS 334
+ +NL DD E R+SL P +T+S D+ N LQ T V DS
Sbjct: 114 ENVNLPDDAKDPEISRYSLNSFPILTLSVSSDKDN--------------LQELTKQVEDS 159
Query: 335 LMRLLYGLE 361
L+ L G+E
Sbjct: 160 LVSKLEGIE 168
>sp|Q5MZM0|Y2310_SYNP6 Hypothetical UPF0182 protein syc2310_c
Length = 955
Score = 33.9 bits (76), Expect = 0.54
Identities = 16/59 (27%), Positives = 31/59 (52%), Gaps = 2/59 (3%)
Frame = +2
Query: 458 LIDPKHPLVLSWERMFNRYLQ--NVRTTKIRADPRDPEFVLYSDAEVIVTAHRVKPALF 628
++DPK PL+ +W R+F Q + +R+ R P + + + ++T H + P +F
Sbjct: 703 IVDPKDPLIQTWSRLFPSLFQPIDAMAPVLRSHLRYPTDLFKAQSSQLLTYHVLDPQVF 761
>sp|Q68XE8|SYT_RICTY Threonyl-tRNA synthetase (Threonine--tRNA ligase) (ThrRS)
Length = 635
Score = 32.0 bits (71), Expect = 2.1
Identities = 17/57 (29%), Positives = 32/57 (56%)
Frame = +2
Query: 104 ALNYTATLFNSSVNIVHKRINLNDDELQWEHERFSLRKIPAITISAEKDRLNNRKSI 274
AL TL + N + IN++ D++ ++ FS +KIP I + +K++ N + +I
Sbjct: 554 ALEVQKTLID---NNIRTDINISPDKINYKIREFSNQKIPMIAVIGKKEQANKQVTI 607
>sp|P22003|BMP5_HUMAN Bone morphogenetic protein 5 precursor (BMP-5)
Length = 454
Score = 30.8 bits (68), Expect = 4.6
Identities = 33/128 (25%), Positives = 53/128 (41%), Gaps = 14/128 (10%)
Frame = +2
Query: 119 ATLFNSSVNIVHKRINLNDDELQWEHERFSLRKIP------AITISAEKDRLNNRKSILD 280
A + S VN+V + + + ++ RF L +IP A KDR NNR
Sbjct: 154 ADMVMSFVNLVERDKDFSHQRRHYKEFRFDLTQIPHGEAVTAAEFRIYKDRSNNRFENET 213
Query: 281 DRISV-ELLQRNTLSVADSLM---RLLYGLEDG----HGPITEDHWVSEEAINADLALLT 436
+IS+ ++++ T AD + R L+ G +T +HWV N L L
Sbjct: 214 IKISIYQIIKEYTNRDADLFLLDTRKAQALDVGWLVFDITVTSNHWVINPQNNLGLQLCA 273
Query: 437 RFPRGQQL 460
G+ +
Sbjct: 274 ETGDGRSI 281
>sp|P54675|PI3K3_DICDI Phosphatidylinositol 3-kinase 3 (PI3-kinase) (PtdIns-3-kinase)
(PI3K)
Length = 1585
Score = 30.8 bits (68), Expect = 4.6
Identities = 25/95 (26%), Positives = 44/95 (46%), Gaps = 7/95 (7%)
Frame = +2
Query: 131 NSSVNIVHKRINLNDDELQWEHERFSLRKIPAITISAEKDRLNN--RKSILDDRISVELL 304
N+ I + N ND+ + E SL+++ EK++L N R+ IL +R ++ L
Sbjct: 377 NNEELINNNNNNNNDENYKIEETEESLKEL------LEKEKLENEEREKILKERNEIDNL 430
Query: 305 QRNTLSVADSLMRLLY-----GLEDGHGPITEDHW 394
++ MR GLE+ P+ ++HW
Sbjct: 431 KKKNHLSKGYFMRACNASNDDGLEEEDIPLQDEHW 465
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 94,553,804
Number of Sequences: 369166
Number of extensions: 1953388
Number of successful extensions: 4837
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4697
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4825
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 7763237265
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)