Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_006_D04
(825 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P39702|VPS8_YEAST Vacuolar protein sorting-associated pr... 34 0.43
sp|Q9DD48|MKRN2_SERQU Makorin-2 (Zinc finger protein YGHLC3... 33 0.73
sp|Q9ERV1|MKRN2_MOUSE Makorin-2 33 0.73
sp|P51835|FTSY_BACSU Cell division protein ftsY homolog 33 0.95
sp|P06167|HEMA_PI3B Hemagglutinin-neuraminidase 33 1.2
sp|Q9H000|MKRN2_HUMAN Makorin-2 (RING finger protein 62) 32 1.6
sp|P27801|PEP3_YEAST Vacuolar membrane protein PEP3 32 1.6
sp|Q980L3|SYH_SULSO Histidyl-tRNA synthetase (Histidine--tR... 32 1.6
sp|Q9UHC7|MKRN1_HUMAN Makorin-1 (RING finger protein 61) 32 2.1
sp|Q9VGI8|BLM_DROME Bloom's syndrome protein homolog (Dmblm... 32 2.1
>sp|P39702|VPS8_YEAST Vacuolar protein sorting-associated protein VPS8
Length = 1274
Score = 34.3 bits (77), Expect = 0.43
Identities = 56/244 (22%), Positives = 99/244 (40%), Gaps = 23/244 (9%)
Frame = -3
Query: 805 HEGYIELLVGICDVVKLIQIAL---QTSNFQILERIK----DCVF---NSKNSFQQAIER 656
HE + + D++ I+ L +T+ +LE ++ DC K+ + I
Sbjct: 1023 HERLESFNLAVRDLLSFIEQCLNEGKTNISTLLESLRRAFDDCNSAGTEKKSCWILLITF 1082
Query: 655 LILLYRKY--HYDHCICCNRIIENRFTD---PQPVLFKDIGELIFNKLIDKNNHQEIHHN 491
LI LY KY H + CN++++ F + KD G + + HQ++
Sbjct: 1083 LITLYGKYPSHDERKDLCNKLLQEAFLGLVRSKSSSQKDSGGEFWEIMSSVLEHQDV--- 1139
Query: 490 IIKMLNKNEVDQVKEYLIISIHNIINTRKITVRNQMFNFIPNEINQVEESSKLTEMFLNI 311
+L K V +K+ L+ N+ NT K+ + I I + S L + +
Sbjct: 1140 ---ILMK--VQDLKQLLL----NVFNTYKL--ERSLSELIQKIIE--DSSQDLVQQYRKF 1186
Query: 310 YGMSYS------ELCGEN-WGKILSQNMYCDICGAVLIDKMLTSN-ELNIVSFSCSHTFH 155
+S E+CG+ WG L ++ M++ + + +V F C H FH
Sbjct: 1187 LSEGWSIHTDDCEICGKKIWGAGLDPLLFLAWENVQRHQDMISVDLKTPLVIFKCHHGFH 1246
Query: 154 RKCL 143
+ CL
Sbjct: 1247 QTCL 1250
>sp|Q9DD48|MKRN2_SERQU Makorin-2 (Zinc finger protein YGHLC3HC4)
Length = 423
Score = 33.5 bits (75), Expect = 0.73
Identities = 16/42 (38%), Positives = 23/42 (54%)
Frame = -3
Query: 262 LSQNMYCDICGAVLIDKMLTSNELNIVSFSCSHTFHRKCLRE 137
LSQ+ C IC V++ K S+ + SC HTF C+R+
Sbjct: 229 LSQDKVCSICMEVVVQKANPSDRRFGILSSCCHTFCLACIRK 270
>sp|Q9ERV1|MKRN2_MOUSE Makorin-2
Length = 416
Score = 33.5 bits (75), Expect = 0.73
Identities = 14/41 (34%), Positives = 23/41 (56%)
Frame = -3
Query: 259 SQNMYCDICGAVLIDKMLTSNELNIVSFSCSHTFHRKCLRE 137
SQ+ C IC V+++K S + +CSHT+ C+R+
Sbjct: 233 SQDKVCSICMEVILEKASASERRFGILSNCSHTYCLSCIRQ 273
>sp|P51835|FTSY_BACSU Cell division protein ftsY homolog
Length = 329
Score = 33.1 bits (74), Expect = 0.95
Identities = 30/111 (27%), Positives = 51/111 (45%), Gaps = 1/111 (0%)
Frame = -3
Query: 751 QIALQTSNFQILERIKDCVFNSKNSFQQAIERLILLYRKYHYDHCICCNRIIENRFTDPQ 572
+I QT + + E+ KD + ++NSFQ + L+ YRK + E+ F + +
Sbjct: 10 KITKQTDS--VSEKFKDGLEKTRNSFQNKVNDLVSRYRK-----------VDEDFFEELE 56
Query: 571 PVLFK-DIGELIFNKLIDKNNHQEIHHNIIKMLNKNEVDQVKEYLIISIHN 422
VL D+G +LID+ + NI + EV V ++ I+N
Sbjct: 57 EVLISADVGFTTVMELIDELKKEVKRRNI---QDPKEVQSVISEKLVEIYN 104
>sp|P06167|HEMA_PI3B Hemagglutinin-neuraminidase
Length = 572
Score = 32.7 bits (73), Expect = 1.2
Identities = 21/69 (30%), Positives = 36/69 (52%), Gaps = 5/69 (7%)
Frame = -3
Query: 544 LIFNKLIDKNNH-----QEIHHNIIKMLNKNEVDQVKEYLIISIHNIINTRKITVRNQMF 380
+I LI +NNH QEI + K + + + + SI + INTR +T+++ +
Sbjct: 50 MILINLIQENNHNKLMLQEIRKEFAAIDTK--IQKTSDDIGTSIQSGINTRLLTIQSHVQ 107
Query: 379 NFIPNEINQ 353
N+IP + Q
Sbjct: 108 NYIPLSLTQ 116
>sp|Q9H000|MKRN2_HUMAN Makorin-2 (RING finger protein 62)
Length = 416
Score = 32.3 bits (72), Expect = 1.6
Identities = 13/41 (31%), Positives = 23/41 (56%)
Frame = -3
Query: 259 SQNMYCDICGAVLIDKMLTSNELNIVSFSCSHTFHRKCLRE 137
SQ+ C IC V+++K S + +C+HT+ C+R+
Sbjct: 233 SQDKVCSICMEVILEKASASERRFGILSNCNHTYCLSCIRQ 273
>sp|P27801|PEP3_YEAST Vacuolar membrane protein PEP3
Length = 918
Score = 32.3 bits (72), Expect = 1.6
Identities = 35/138 (25%), Positives = 52/138 (37%)
Frame = -3
Query: 556 DIGELIFNKLIDKNNHQEIHHNIIKMLNKNEVDQVKEYLIISIHNIINTRKITVRNQMFN 377
DI +LI L D N I + + +KE LI + N N
Sbjct: 740 DIKQLIRTILNDSNEILTIKDLLPFFNEYTTIANLKEELIKFLEN-------------HN 786
Query: 376 FIPNEINQVEESSKLTEMFLNIYGMSYSELCGENWGKILSQNMYCDICGAVLIDKMLTSN 197
NEI++ +SK ++ +N ++E+ +IL CD CG L K
Sbjct: 787 MKMNEISEDIINSKNLKVEINTEISKFNEIY-----RILEPGKSCDECGKFLQIK----- 836
Query: 196 ELNIVSFSCSHTFHRKCL 143
+ F C H FH C+
Sbjct: 837 --KFIVFPCGHCFHWNCI 852
>sp|Q980L3|SYH_SULSO Histidyl-tRNA synthetase (Histidine--tRNA ligase) (HisRS)
Length = 426
Score = 32.3 bits (72), Expect = 1.6
Identities = 20/74 (27%), Positives = 41/74 (55%), Gaps = 1/74 (1%)
Frame = -3
Query: 541 IFNKLIDKNNHQE-IHHNIIKMLNKNEVDQVKEYLIISIHNIINTRKITVRNQMFNFIPN 365
IF K++DK N ++ + +++ +++KN+VD E L+I NI N + N +
Sbjct: 166 IFRKIMDKYNIEDNLQEHVLHLIDKNKVD---EALVILEKNIKNKDIMDFLNMILTKKEA 222
Query: 364 EINQVEESSKLTEM 323
++ +E ++L E+
Sbjct: 223 KLEDIESLAELEEV 236
>sp|Q9UHC7|MKRN1_HUMAN Makorin-1 (RING finger protein 61)
Length = 482
Score = 32.0 bits (71), Expect = 2.1
Identities = 14/41 (34%), Positives = 24/41 (58%)
Frame = -3
Query: 259 SQNMYCDICGAVLIDKMLTSNELNIVSFSCSHTFHRKCLRE 137
S++M C IC V+ +K S + +C+HT+ KC+R+
Sbjct: 276 SKDMVCGICMEVVYEKANPSERRFGILSNCNHTYCLKCIRK 316
>sp|Q9VGI8|BLM_DROME Bloom's syndrome protein homolog (Dmblm) (Mutagen-sensitive protein
309) (RecQ helicase homolog)
Length = 1487
Score = 32.0 bits (71), Expect = 2.1
Identities = 12/33 (36%), Positives = 23/33 (69%)
Frame = -3
Query: 532 KLIDKNNHQEIHHNIIKMLNKNEVDQVKEYLII 434
K+ID N+H+ HH ++K +KN+V ++ ++I
Sbjct: 1182 KIIDFNHHKTPHHGVLKDWDKNDVHRLLRKMVI 1214
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 80,108,731
Number of Sequences: 369166
Number of extensions: 1476353
Number of successful extensions: 4321
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4158
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4319
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 7956112725
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)