Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_005_O21
(396 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O13035|SAP_CHICK Proactivator polypeptide precursor [Con... 51 7e-07
sp|P10960|SAP_RAT Sulfated glycoprotein 1 precursor (SGP-1)... 49 4e-06
sp|Q61207|SAP_MOUSE Sulfated glycoprotein 1 precursor (SGP-... 47 2e-05
sp|P07602|SAP_HUMAN Proactivator polypeptide precursor [Con... 46 2e-05
sp|P26779|SAP_BOVIN Proactivator polypeptide precursor [Con... 46 2e-05
sp|P50405|PSPB_MOUSE Pulmonary surfactant-associated protei... 38 0.008
sp|P17129|PSPB_CANFA Pulmonary surfactant-associated protei... 34 0.12
sp|Q5DU02|UBP22_MOUSE Ubiquitin carboxyl-terminal hydrolase... 31 1.0
sp|Q9UPT9|UBP22_HUMAN Ubiquitin carboxyl-terminal hydrolase... 31 1.0
sp|Q6EW41|CEMA_NYMAL Chloroplast envelope membrane protein 30 1.7
>sp|O13035|SAP_CHICK Proactivator polypeptide precursor [Contains: Saposin A; Saposin B;
Saposin C; Saposin D]
Length = 518
Score = 51.2 bits (121), Expect = 7e-07
Identities = 20/50 (40%), Positives = 28/50 (56%)
Frame = +2
Query: 113 CEMTKMCSHQRKNPLGKNPCTWGPGFWCVSRKHAVACGPGAVKHCEMNVW 262
C +C +K LG++ C WGPG+WC + + A C AV HC +VW
Sbjct: 470 CTKLGVCGAAKKPLLGEDACVWGPGYWCKNMETAAQC--NAVDHCRRHVW 517
Score = 40.8 bits (94), Expect = 0.001
Identities = 18/36 (50%), Positives = 21/36 (58%)
Frame = +2
Query: 155 LGKNPCTWGPGFWCVSRKHAVACGPGAVKHCEMNVW 262
L + C GP WC S + A CG AVKHC+ NVW
Sbjct: 21 LWQKDCAKGPEVWCQSLRTASQCG--AVKHCQQNVW 54
>sp|P10960|SAP_RAT Sulfated glycoprotein 1 precursor (SGP-1) (Prosaposin)
Length = 554
Score = 48.9 bits (115), Expect = 4e-06
Identities = 20/50 (40%), Positives = 26/50 (52%)
Frame = +2
Query: 113 CEMTKMCSHQRKNPLGKNPCTWGPGFWCVSRKHAVACGPGAVKHCEMNVW 262
C +C K LG C WGPG+WC + + A C AV HC+ +VW
Sbjct: 506 CSKIGVCPSAYKLLLGTEKCVWGPGYWCQNSETAARC--NAVDHCKRHVW 553
Score = 27.7 bits (60), Expect = 8.6
Identities = 14/31 (45%), Positives = 16/31 (51%)
Frame = +2
Query: 170 CTWGPGFWCVSRKHAVACGPGAVKHCEMNVW 262
C+ G C K AV C AVKHC+ VW
Sbjct: 25 CSGGSAVVCRDVKTAVDCR--AVKHCQQMVW 53
>sp|Q61207|SAP_MOUSE Sulfated glycoprotein 1 precursor (SGP-1) (Prosaposin)
Length = 557
Score = 46.6 bits (109), Expect = 2e-05
Identities = 19/50 (38%), Positives = 25/50 (50%)
Frame = +2
Query: 113 CEMTKMCSHQRKNPLGKNPCTWGPGFWCVSRKHAVACGPGAVKHCEMNVW 262
C +C K LG C WGP +WC + + A C AV HC+ +VW
Sbjct: 509 CSKIGVCPSAYKLLLGTEKCVWGPSYWCQNMETAARC--NAVDHCKRHVW 556
Score = 31.2 bits (69), Expect = 0.78
Identities = 15/31 (48%), Positives = 17/31 (54%)
Frame = +2
Query: 170 CTWGPGFWCVSRKHAVACGPGAVKHCEMNVW 262
C+ G C K AV CG AVKHC+ VW
Sbjct: 25 CSGGSAVLCRDVKTAVDCG--AVKHCQQMVW 53
>sp|P07602|SAP_HUMAN Proactivator polypeptide precursor [Contains: Saposin A (Protein
A); Saposin B-Val; Saposin B (Sphingolipid activator
protein 1) (SAP-1) (Cerebroside sulfate activator)
(CSAct) (Dispersin) (Sulfatide/GM1 activator); Saposin C
(Co-beta-glucosidase) (A1 activator) (Glucosylceramidase
activator) (Sphingolipid activator protein 2) (SAP-2);
Saposin D (Protein C) (Component C)]
Length = 524
Score = 46.2 bits (108), Expect = 2e-05
Identities = 19/50 (38%), Positives = 25/50 (50%)
Frame = +2
Query: 113 CEMTKMCSHQRKNPLGKNPCTWGPGFWCVSRKHAVACGPGAVKHCEMNVW 262
C C K LG C WGP +WC + + A C AV+HC+ +VW
Sbjct: 476 CLKIGACPSAHKPLLGTEKCIWGPSYWCQNTETAAQC--NAVEHCKRHVW 523
Score = 37.7 bits (86), Expect = 0.008
Identities = 18/38 (47%), Positives = 20/38 (52%)
Frame = +2
Query: 155 LGKNPCTWGPGFWCVSRKHAVACGPGAVKHCEMNVWRQ 268
LG CT G WC + K A C GAVKHC VW +
Sbjct: 20 LGLKECTRGSAVWCQNVKTASDC--GAVKHCLQTVWNK 55
>sp|P26779|SAP_BOVIN Proactivator polypeptide precursor [Contains: Saposin A (Protein
A); Saposin B (Sphingolipid activator protein 1) (SAP-1)
(Cerebroside sulfate activator) (CSAct) (Dispersin)
(Sulfatide/GM1 activator); Saposin C
(Co-beta-glucosidase) (A1 activator) (Glucosylceramidase
activator) (Sphingolipid activator protein 2) (SAP-2);
Saposin D (Protein C) (Component C)]
Length = 525
Score = 46.2 bits (108), Expect = 2e-05
Identities = 19/50 (38%), Positives = 24/50 (48%)
Frame = +2
Query: 113 CEMTKMCSHQRKNPLGKNPCTWGPGFWCVSRKHAVACGPGAVKHCEMNVW 262
C C K LG C WGP +WC + + A C AV+HC +VW
Sbjct: 477 CLKIGACPAAHKPLLGAEKCVWGPSYWCQNMESAALC--NAVEHCRRHVW 524
Score = 36.6 bits (83), Expect = 0.019
Identities = 17/36 (47%), Positives = 19/36 (52%)
Frame = +2
Query: 155 LGKNPCTWGPGFWCVSRKHAVACGPGAVKHCEMNVW 262
LG CT G WC + K A C GAV+HC VW
Sbjct: 20 LGLRECTRGSAVWCQNVKTAADC--GAVQHCLQTVW 53
>sp|P50405|PSPB_MOUSE Pulmonary surfactant-associated protein B precursor (SP-B)
(Pulmonary surfactant-associated proteolipid SPL(Phe))
Length = 377
Score = 37.7 bits (86), Expect = 0.008
Identities = 16/31 (51%), Positives = 18/31 (58%)
Frame = +2
Query: 170 CTWGPGFWCVSRKHAVACGPGAVKHCEMNVW 262
C GP FWC S +HAV C A+ HC VW
Sbjct: 31 CAQGPQFWCQSLEHAVQC--RALGHCLQEVW 59
>sp|P17129|PSPB_CANFA Pulmonary surfactant-associated protein B precursor (SP-B) (6 kDa
protein) (Pulmonary surfactant-associated proteolipid
SPL(Phe)) (Pulmonary surfactant protein 18) (SP 18)
Length = 363
Score = 33.9 bits (76), Expect = 0.12
Identities = 14/31 (45%), Positives = 17/31 (54%)
Frame = +2
Query: 170 CTWGPGFWCVSRKHAVACGPGAVKHCEMNVW 262
C GP FWC S + A+ C A+ HC VW
Sbjct: 25 CARGPAFWCQSLEQALQC--RALGHCLQEVW 53
>sp|Q5DU02|UBP22_MOUSE Ubiquitin carboxyl-terminal hydrolase 22 (Ubiquitin thiolesterase
22) (Ubiquitin-specific processing protease 22)
(Deubiquitinating enzyme 22)
Length = 525
Score = 30.8 bits (68), Expect = 1.0
Identities = 15/40 (37%), Positives = 22/40 (55%), Gaps = 3/40 (7%)
Frame = -3
Query: 334 CELKNSSSL---KSSIRIVELYNGHLSPHIHFTMFHCTGT 224
CE+++ SS + S E Y+GH SPHI + + H T
Sbjct: 211 CEMQSPSSCLVCEMSSLFQEFYSGHRSPHIPYKLLHLVWT 250
>sp|Q9UPT9|UBP22_HUMAN Ubiquitin carboxyl-terminal hydrolase 22 (Ubiquitin thiolesterase
22) (Ubiquitin-specific processing protease 22)
(Deubiquitinating enzyme 22)
Length = 525
Score = 30.8 bits (68), Expect = 1.0
Identities = 15/40 (37%), Positives = 22/40 (55%), Gaps = 3/40 (7%)
Frame = -3
Query: 334 CELKNSSSL---KSSIRIVELYNGHLSPHIHFTMFHCTGT 224
CE+++ SS + S E Y+GH SPHI + + H T
Sbjct: 211 CEMQSPSSCLVCEMSSLFQEFYSGHRSPHIPYKLLHLVWT 250
>sp|Q6EW41|CEMA_NYMAL Chloroplast envelope membrane protein
Length = 229
Score = 30.0 bits (66), Expect = 1.7
Identities = 12/38 (31%), Positives = 22/38 (57%)
Frame = -3
Query: 307 KSSIRIVELYNGHLSPHIHFTMFHCTGTTCYSVFPAYT 194
K +I++V ++N HIH + T TC+++ AY+
Sbjct: 90 KETIQLVRMHN---QDHIHIILHFSTNITCFAILSAYS 124
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 45,220,133
Number of Sequences: 369166
Number of extensions: 911949
Number of successful extensions: 1950
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1892
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1945
length of database: 68,354,980
effective HSP length: 97
effective length of database: 50,435,685
effective search space used: 1714813290
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)