Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_005_O20
(677 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P54611|VATE_DROME Vacuolar ATP synthase subunit E (V-ATP... 182 5e-46
sp|P31402|VATE_MANSE Vacuolar ATP synthase subunit E (V-ATP... 173 4e-43
sp|P36543|VATE_HUMAN Vacuolar ATP synthase subunit E (V-ATP... 167 2e-41
sp|P11019|VATE_BOVIN Vacuolar ATP synthase subunit E (V-ATP... 166 7e-41
sp|Q9U1G5|VATE_HETSC Vacuolar ATP synthase subunit E (V-ATP... 155 7e-38
sp|P50518|VATE_MOUSE Vacuolar ATP synthase subunit E (V-ATP... 151 1e-36
sp|O94072|VATE_CANAL Vacuolar ATP synthase subunit E (V-ATP... 114 3e-25
sp|Q01278|VATE_NEUCR Vacuolar ATP synthase subunit E (V-ATP... 113 4e-25
sp|O00780|VATE_DICDI Vacuolar ATP synthase subunit E (V-ATP... 112 9e-25
sp|Q39258|VATE_ARATH Vacuolar ATP synthase subunit E (V-ATP... 106 6e-23
>sp|P54611|VATE_DROME Vacuolar ATP synthase subunit E (V-ATPase E subunit) (Vacuolar
proton pump E subunit) (V-ATPase 28 kDa subunit)
Length = 226
Score = 182 bits (463), Expect = 5e-46
Identities = 92/177 (51%), Positives = 125/177 (70%)
Frame = +2
Query: 2 RSKMNEFFSKEEKKLEQRKKIQHSNMMNQARLKILGCQQEHVKDVLTEAQKRLHNLTNDP 181
R K+ E++ K+EK++E +KKIQ SNM+NQARLK+L +++HV VL +A+KRL +T +
Sbjct: 50 RLKIMEYYEKKEKQVELQKKIQSSNMLNQARLKVLKVREDHVSSVLDDARKRLGEVTKNQ 109
Query: 182 QKYKETLKRLITQGLFQLLEAKVIIRCCRGDIRLVEVILDECASAYEAKTEMKVELTINK 361
+Y+ L +LI QGLFQ++E KVI+RC D+ LV +L Y+A+ VEL I++
Sbjct: 110 SEYETVLTKLIVQGLFQIMEPKVILRCREVDVPLVRNVLPAAVEQYKAQINQNVELFIDE 169
Query: 362 DNCLPDDLAGGVELCVPNGRVKIVNTLESRLDLISQQLIPEIREILFGKNTNRIFND 532
+ L D GGVEL NGR+K+ NTLESRLDLISQQL+PEIR LFG+N NR F D
Sbjct: 170 KDFLSADTCGGVELLALNGRIKVPNTLESRLDLISQQLVPEIRNALFGRNVNRKFTD 226
>sp|P31402|VATE_MANSE Vacuolar ATP synthase subunit E (V-ATPase E subunit) (Vacuolar
proton pump E subunit) (V-ATPase 28 kDa subunit)
Length = 226
Score = 173 bits (438), Expect = 4e-43
Identities = 90/177 (50%), Positives = 122/177 (68%)
Frame = +2
Query: 2 RSKMNEFFSKEEKKLEQRKKIQHSNMMNQARLKILGCQQEHVKDVLTEAQKRLHNLTNDP 181
R K+ E++ K+EK++E +KKIQ SNM+NQARLK+L +++HV++VL EA+KRL + D
Sbjct: 50 RLKIMEYYEKKEKQVELQKKIQSSNMLNQARLKVLKVREDHVRNVLDEARKRLAEVPKDI 109
Query: 182 QKYKETLKRLITQGLFQLLEAKVIIRCCRGDIRLVEVILDECASAYEAKTEMKVELTINK 361
+ Y + L LI Q LFQL+E V +R + D LVE +L Y+AK + V L I+
Sbjct: 110 KLYSDLLVTLIVQALFQLVEPTVTLRVRQADKALVESLLGRAQQDYKAKIKKDVVLKIDN 169
Query: 362 DNCLPDDLAGGVELCVPNGRVKIVNTLESRLDLISQQLIPEIREILFGKNTNRIFND 532
+N LP D GG+EL GR+KI NTLESRL+LI+QQL+PEIR LFG+N NR F D
Sbjct: 170 ENFLPPDTCGGIELIAAKGRIKISNTLESRLELIAQQLLPEIRNALFGRNPNRKFTD 226
>sp|P36543|VATE_HUMAN Vacuolar ATP synthase subunit E (V-ATPase E subunit) (Vacuolar
proton pump E subunit) (V-ATPase 31 kDa subunit) (P31)
Length = 226
Score = 167 bits (423), Expect = 2e-41
Identities = 81/177 (45%), Positives = 127/177 (71%)
Frame = +2
Query: 2 RSKMNEFFSKEEKKLEQRKKIQHSNMMNQARLKILGCQQEHVKDVLTEAQKRLHNLTNDP 181
R K+ E++ K+EK++EQ+KKIQ SN+MNQARLK+L + + + D+L EA++RL + D
Sbjct: 50 RLKIMEYYEKKEKQIEQQKKIQMSNLMNQARLKVLRARDDLITDLLNEAKQRLSKVVKDT 109
Query: 182 QKYKETLKRLITQGLFQLLEAKVIIRCCRGDIRLVEVILDECASAYEAKTEMKVELTINK 361
+Y+ L L+ QGL+QLLE ++I+RC + D LV+ + + Y+ T+ V++ I++
Sbjct: 110 TRYQVLLDGLVLQGLYQLLEPRMIVRCRKQDFPLVKAAVQKAIPMYKIATKNDVDVQIDQ 169
Query: 362 DNCLPDDLAGGVELCVPNGRVKIVNTLESRLDLISQQLIPEIREILFGKNTNRIFND 532
++ LP+D+AGGVE+ + ++K+ NTLESRLDLI+QQ++PE+R LFG N NR F D
Sbjct: 170 ESYLPEDIAGGVEIYNGDRKIKVSNTLESRLDLIAQQMMPEVRGALFGANANRKFLD 226
>sp|P11019|VATE_BOVIN Vacuolar ATP synthase subunit E (V-ATPase E subunit) (Vacuolar
proton pump E subunit) (V-ATPase 31 kDa subunit) (P31)
Length = 226
Score = 166 bits (419), Expect = 7e-41
Identities = 80/177 (45%), Positives = 126/177 (71%)
Frame = +2
Query: 2 RSKMNEFFSKEEKKLEQRKKIQHSNMMNQARLKILGCQQEHVKDVLTEAQKRLHNLTNDP 181
R K+ E++ K+EK++EQ+KKIQ SN+MNQARLK+L + + + D+L EA++RL + D
Sbjct: 50 RLKIMEYYEKKEKQIEQQKKIQMSNLMNQARLKVLRARDDLITDLLNEAKQRLSKVVKDT 109
Query: 182 QKYKETLKRLITQGLFQLLEAKVIIRCCRGDIRLVEVILDECASAYEAKTEMKVELTINK 361
+Y+ L L+ QGL+QLLE ++I+RC + D LV+ + + Y+ T+ V++ I++
Sbjct: 110 TRYQVLLDGLVLQGLYQLLEPRMIVRCRKQDFPLVKAAVQKAIPVYKVATKRDVDVQIDQ 169
Query: 362 DNCLPDDLAGGVELCVPNGRVKIVNTLESRLDLISQQLIPEIREILFGKNTNRIFND 532
+ LP+++AGGVE+ + ++K+ NTLESRLDLI+QQ++PE+R LFG N NR F D
Sbjct: 170 EAYLPEEIAGGVEIYNGDRKIKVSNTLESRLDLIAQQMMPEVRGALFGANANRKFLD 226
>sp|Q9U1G5|VATE_HETSC Vacuolar ATP synthase subunit E (V-ATPase E subunit) (Vacuolar
proton pump E subunit)
Length = 226
Score = 155 bits (393), Expect = 7e-38
Identities = 77/177 (43%), Positives = 120/177 (67%)
Frame = +2
Query: 2 RSKMNEFFSKEEKKLEQRKKIQHSNMMNQARLKILGCQQEHVKDVLTEAQKRLHNLTNDP 181
R K+ E++ K+EK++E ++KIQ SNM NQ+RLK L + +H+K+VL EA+ L ++ D
Sbjct: 50 RQKIMEYYEKKEKQVELQRKIQRSNMQNQSRLKCLKARDDHLKNVLEEARANLSKISADR 109
Query: 182 QKYKETLKRLITQGLFQLLEAKVIIRCCRGDIRLVEVILDECASAYEAKTEMKVELTINK 361
++Y LK L+ QGLFQLLE+KV++RC + D +V IL EC + + E+ I+
Sbjct: 110 ERYPAILKGLLLQGLFQLLESKVVLRCRKKDEEMVARILPECLEEVQRTWGNRSEVKIDN 169
Query: 362 DNCLPDDLAGGVELCVPNGRVKIVNTLESRLDLISQQLIPEIREILFGKNTNRIFND 532
++ L + AGGVEL +G++++ +TLE+RLDLI+ ++ P++R LFG N NR F D
Sbjct: 170 EHFLSPESAGGVELLAKDGKIRVSSTLEARLDLIADKITPQVRTALFGPNQNRRFFD 226
>sp|P50518|VATE_MOUSE Vacuolar ATP synthase subunit E (V-ATPase E subunit) (Vacuolar
proton pump E subunit) (V-ATPase 31 kDa subunit) (P31)
Length = 228
Score = 151 bits (382), Expect = 1e-36
Identities = 77/178 (43%), Positives = 123/178 (69%), Gaps = 1/178 (0%)
Frame = +2
Query: 2 RSKMNEFFSKEEKKL-EQRKKIQHSNMMNQARLKILGCQQEHVKDVLTEAQKRLHNLTND 178
R K+ E++ K+EK++ +Q+KKIQ SN+MNQARLK+L + + + D+L EA++RL + D
Sbjct: 51 RLKIMEYYEKKEKQIRQQQKKIQMSNLMNQARLKVLRARDDLITDLLNEAKQRLMKVVKD 110
Query: 179 PQKYKETLKRLITQGLFQLLEAKVIIRCCRGDIRLVEVILDECASAYEAKTEMKVELTIN 358
+Y+ L L+ QGL+QLLE ++I+RC + D LV+ + + Y+ T+ V++ I+
Sbjct: 111 TTRYQVLLDGLVLQGLYQLLEPRMIVRCRKQDFPLVKAAVQKAIPMYKIATKRDVDVQID 170
Query: 359 KDNCLPDDLAGGVELCVPNGRVKIVNTLESRLDLISQQLIPEIREILFGKNTNRIFND 532
++ ++AGGVE+ + ++K+ NTLESRLDLI+QQ++PE+R LFG N NR F D
Sbjct: 171 QEPTCLRNIAGGVEIYNGDRKIKVSNTLESRLDLIAQQMMPEVRGALFGANANRKFLD 228
>sp|O94072|VATE_CANAL Vacuolar ATP synthase subunit E (V-ATPase E subunit) (Vacuolar
proton pump E subunit)
Length = 226
Score = 114 bits (284), Expect = 3e-25
Identities = 59/170 (34%), Positives = 102/170 (60%)
Frame = +2
Query: 23 FSKEEKKLEQRKKIQHSNMMNQARLKILGCQQEHVKDVLTEAQKRLHNLTNDPQKYKETL 202
+ ++ KK ++I S + N+ RL+IL + E + ++ EA+ L +T D ++YK L
Sbjct: 57 YEQKLKKASLAQQITKSTIGNKTRLRILSTKDEVLHEIFDEAEAELKKITKDKKQYKPVL 116
Query: 203 KRLITQGLFQLLEAKVIIRCCRGDIRLVEVILDECASAYEAKTEMKVELTINKDNCLPDD 382
LI +G+ L+E KV I+ D+ + + + E A +E K + KVE++I+ N L D
Sbjct: 117 VGLIEEGVLALMEPKVSIKVREQDVDVAKEAITEAAKNFEEKAKFKVEISIDDKNFLAKD 176
Query: 383 LAGGVELCVPNGRVKIVNTLESRLDLISQQLIPEIREILFGKNTNRIFND 532
+AGG+ + +G++++ NTLE RL ++S++ +P IR LFG +T R F D
Sbjct: 177 IAGGIVVVNGSGKIEVDNTLEERLKILSEEALPAIRLELFGPSTTRKFFD 226
>sp|Q01278|VATE_NEUCR Vacuolar ATP synthase subunit E (V-ATPase E subunit) (Vacuolar
proton pump E subunit) (V-ATPase 26 kDa subunit)
Length = 230
Score = 113 bits (283), Expect = 4e-25
Identities = 57/170 (33%), Positives = 102/170 (60%)
Frame = +2
Query: 23 FSKEEKKLEQRKKIQHSNMMNQARLKILGCQQEHVKDVLTEAQKRLHNLTNDPQKYKETL 202
++K+ K+ + ++I S M N+ RL++LG +QE + ++ A +L T+D +YK+ L
Sbjct: 61 YAKKFKQAQMSQQITRSTMANKTRLRVLGARQELLDEIFEAASAQLGQATHDLGRYKDIL 120
Query: 203 KRLITQGLFQLLEAKVIIRCCRGDIRLVEVILDECASAYEAKTEMKVELTINKDNCLPDD 382
+ LI +G + + E +++IR + D V ++ Y+ KT+ V+ TI+ +N +P+
Sbjct: 121 RDLILEGFYAMNEPELVIRARQADYDAVREAAGWASAQYKHKTDKDVKATIDAENPVPEG 180
Query: 383 LAGGVELCVPNGRVKIVNTLESRLDLISQQLIPEIREILFGKNTNRIFND 532
AGG+ + NG++ I NT E+RL L+ +P +R+ LFG+N NR F D
Sbjct: 181 SAGGIIIVGGNGKIDIDNTFEARLTLLKDSALPAMRKALFGENPNRKFFD 230
>sp|O00780|VATE_DICDI Vacuolar ATP synthase subunit E (V-ATPase E subunit) (Vacuolar
proton pump E subunit)
Length = 233
Score = 112 bits (280), Expect = 9e-25
Identities = 65/184 (35%), Positives = 107/184 (58%), Gaps = 9/184 (4%)
Frame = +2
Query: 8 KMNEFFSKEEKKLEQRKKIQHSNMMNQARLKILGCQQEHVKDVLTEAQKRLHNLTNDPQK 187
K+ + + K++K +E +KKI SN +N++RL +L ++E ++DV+ EAQK+L +++D K
Sbjct: 50 KIIKEYEKKQKLIEVQKKINLSNELNKSRLSVLKVREECLRDVIKEAQKKLATISDDKDK 109
Query: 188 YKETLKRLITQGLFQLLEAKVIIRCCRGDIRLVEVILDECASAYEAKTEMKVELTINKDN 367
Y+ LK LI QG +L E K+ + + D L+E E A+ Y+ ++++++K+
Sbjct: 110 YQTILKNLIYQGFVKLNENKIQVVGRKEDAGLLEKATTEAAAQYKKNVGKSIDVSVDKER 169
Query: 368 CLPD---------DLAGGVELCVPNGRVKIVNTLESRLDLISQQLIPEIREILFGKNTNR 520
LP GGV L GR+ NTL+SRL++ QL P IR L+G +T+R
Sbjct: 170 FLPQGPKSDYNGPTCCGGVILSALEGRIICKNTLDSRLEICFDQLTPVIRTQLYGASTSR 229
Query: 521 IFND 532
F D
Sbjct: 230 KFFD 233
>sp|Q39258|VATE_ARATH Vacuolar ATP synthase subunit E (V-ATPase E subunit) (Vacuolar
proton pump E subunit)
Length = 230
Score = 106 bits (264), Expect = 6e-23
Identities = 62/182 (34%), Positives = 102/182 (56%), Gaps = 11/182 (6%)
Frame = +2
Query: 2 RSKMNEFFSKEEKKLEQRKKIQHSNMMNQARLKILGCQQEHVKDVLTEAQKRLHNLTNDP 181
+ K+ + + K+EK+ + RKKI +S +N +R+K+L Q + V + +A K L N++ D
Sbjct: 48 KKKIRQDYEKKEKQADVRKKIDYSMQLNASRIKVLQAQDDIVNAMKDQAAKDLLNVSRDE 107
Query: 182 QKYKETLKRLITQGLFQLLEAKVIIRCCRGDIRLVEVILDECASAYEAKTEMKV-ELTIN 358
YK+ LK LI Q L +L E V++RC D+ LVE +LD+ Y K ++ E+ ++
Sbjct: 108 YAYKQLLKDLIVQCLLRLKEPSVLLRCREEDLGLVEAVLDDAKEEYAGKAKVHAPEVAVD 167
Query: 359 KDNCLPD----------DLAGGVELCVPNGRVKIVNTLESRLDLISQQLIPEIREILFGK 508
LP +GGV L +G++ NTL++RLD+ + +P IR+ LFG+
Sbjct: 168 TKIFLPPPPKSNDPHGLHCSGGVVLASRDGKIVCENTLDARLDVAFRMKLPVIRKSLFGQ 227
Query: 509 NT 514
T
Sbjct: 228 VT 229
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 74,159,029
Number of Sequences: 369166
Number of extensions: 1472205
Number of successful extensions: 4277
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4115
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4258
length of database: 68,354,980
effective HSP length: 107
effective length of database: 48,588,335
effective search space used: 5733423530
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)