Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_005_N22
(690 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q99832|TCPH_HUMAN T-complex protein 1, eta subunit (TCP-... 305 6e-83
sp|Q5R5C8|TCPH_PONPY T-complex protein 1, eta subunit (TCP-... 305 1e-82
sp|P80313|TCPH_MOUSE T-complex protein 1, eta subunit (TCP-... 301 1e-81
sp|P87153|TCPH_SCHPO Probable T-complex protein 1, eta subu... 273 4e-73
sp|P42943|TCPH_YEAST T-complex protein 1, eta subunit (TCP-... 247 2e-65
sp|P54409|TCPH_TETPY T-complex protein 1, eta subunit (TCP-... 216 5e-56
sp|O77323|TCPH_PLAF7 T-complex protein 1, eta subunit (TCP-... 209 4e-54
sp|P50016|THS_METKA Thermosome subunit (Chaperonin-like com... 162 6e-40
sp|O28045|THSA_ARCFU Thermosome alpha subunit (Thermosome s... 160 3e-39
sp|P48424|THSA_THEAC Thermosome alpha subunit (Thermosome s... 155 7e-38
>sp|Q99832|TCPH_HUMAN T-complex protein 1, eta subunit (TCP-1-eta) (CCT-eta) (HIV-1 Nef
interacting protein)
Length = 543
Score = 305 bits (782), Expect = 6e-83
Identities = 146/209 (69%), Positives = 174/209 (83%)
Frame = +1
Query: 1 VKDITDDTLGKCESFEEVQIGGERFNIFVGCPEAKTCTIILRGGSEQFMEETERSLHDAI 180
V ++ D LG+C+ FEE QIGGER+N F GCP+AKTCT ILRGG+EQFMEETERSLHDAI
Sbjct: 334 VNALSADVLGRCQVFEETQIGGERYNFFTGCPKAKTCTFILRGGAEQFMEETERSLHDAI 393
Query: 181 MIVRRAMKNDAVVAGGGAIDMEISKYLRDYSRNIHGRDQQFIGAMARAFEVIPRQLCENA 360
MIVRRA+KND+VVAGGGAI+ME+SKYLRDYSR I G+ Q IGA A+A E+IPRQLC+NA
Sbjct: 394 MIVRRAIKNDSVVAGGGAIEMELSKYLRDYSRTIPGKQQLLIGAYAKALEIIPRQLCDNA 453
Query: 361 GFDATNILNKLRQKHAQGKIWCGVDIMNEDIADNFEACVWEPALVKSNAITAATEAACMV 540
GFDATNILNKLR +HAQG W GVDI NEDIADNFEA VWEPA+V+ NA+TAA+EAAC++
Sbjct: 454 GFDATNILNKLRARHAQGGTWYGVDINNEDIADNFEAFVWEPAMVRINALTAASEAACLI 513
Query: 541 LTLDETIRNAKSKTPEDNRPVKMPGRGRP 627
+++DETI+N +S + GRGRP
Sbjct: 514 VSVDETIKNPRSTVDAPTAAGRGRGRGRP 542
>sp|Q5R5C8|TCPH_PONPY T-complex protein 1, eta subunit (TCP-1-eta) (CCT-eta)
Length = 543
Score = 305 bits (780), Expect = 1e-82
Identities = 146/209 (69%), Positives = 174/209 (83%)
Frame = +1
Query: 1 VKDITDDTLGKCESFEEVQIGGERFNIFVGCPEAKTCTIILRGGSEQFMEETERSLHDAI 180
V ++ D LG+C+ FEE QIGGER+N F GCP+AKTCT ILRGG+EQFMEETERSLHDAI
Sbjct: 334 VNALSADVLGRCQVFEETQIGGERYNFFTGCPKAKTCTFILRGGAEQFMEETERSLHDAI 393
Query: 181 MIVRRAMKNDAVVAGGGAIDMEISKYLRDYSRNIHGRDQQFIGAMARAFEVIPRQLCENA 360
MIVRRA+KND+VVAGGGAI+ME+SKYLRDYSR I G+ Q IGA A+A E+IPRQLC+NA
Sbjct: 394 MIVRRAIKNDSVVAGGGAIEMELSKYLRDYSRTIPGKQQLLIGAYAKALEIIPRQLCDNA 453
Query: 361 GFDATNILNKLRQKHAQGKIWCGVDIMNEDIADNFEACVWEPALVKSNAITAATEAACMV 540
GFDATNILNKLR +HAQG W GVDI NEDIADNFEA VWEPA+V+ NA+TAA+EAAC++
Sbjct: 454 GFDATNILNKLRARHAQGGTWYGVDINNEDIADNFEAFVWEPAMVRINALTAASEAACLI 513
Query: 541 LTLDETIRNAKSKTPEDNRPVKMPGRGRP 627
+++DETI+N +S + GRGRP
Sbjct: 514 VSVDETIKNPRSTVDAPAAAGRGRGRGRP 542
>sp|P80313|TCPH_MOUSE T-complex protein 1, eta subunit (TCP-1-eta) (CCT-eta)
Length = 544
Score = 301 bits (770), Expect = 1e-81
Identities = 145/208 (69%), Positives = 174/208 (83%)
Frame = +1
Query: 1 VKDITDDTLGKCESFEEVQIGGERFNIFVGCPEAKTCTIILRGGSEQFMEETERSLHDAI 180
V + D LG C+ FEE QIGGER+N F GCP+AKTCTIILRGG+EQFMEETERSLHDAI
Sbjct: 334 VNALVPDVLGHCQVFEETQIGGERYNFFTGCPKAKTCTIILRGGAEQFMEETERSLHDAI 393
Query: 181 MIVRRAMKNDAVVAGGGAIDMEISKYLRDYSRNIHGRDQQFIGAMARAFEVIPRQLCENA 360
MIVRRA+KND+VVAGGGAI+ME+SKYLRDYSR I G+ Q IGA A+A E+IPRQLC+NA
Sbjct: 394 MIVRRAIKNDSVVAGGGAIEMELSKYLRDYSRTIPGKQQLLIGAYAKALEIIPRQLCDNA 453
Query: 361 GFDATNILNKLRQKHAQGKIWCGVDIMNEDIADNFEACVWEPALVKSNAITAATEAACMV 540
GFDATNILNKLR +HAQG +W GVDI NE+IADNF+A VWEPA+V+ NA+TAA+EAAC++
Sbjct: 454 GFDATNILNKLRARHAQGGMWYGVDINNENIADNFQAFVWEPAMVRINALTAASEAACLI 513
Query: 541 LTLDETIRNAKSKTPEDNRPVKMPGRGR 624
+++DETI+N +S + P GRGR
Sbjct: 514 VSVDETIKNPRSTV---DPPAPSAGRGR 538
>sp|P87153|TCPH_SCHPO Probable T-complex protein 1, eta subunit (TCP-1-eta) (CCT-eta)
Length = 558
Score = 273 bits (697), Expect = 4e-73
Identities = 132/217 (60%), Positives = 171/217 (78%), Gaps = 10/217 (4%)
Frame = +1
Query: 7 DITDDTLGKCESFEEVQIGGERFNIFVGCPEAKTCTIILRGGSEQFMEETERSLHDAIMI 186
+I + LG C++FEE QIGG+RFN+F GCP+AKTCT+ILRGG++QF+ E ERSLHDAIMI
Sbjct: 341 NIEEKHLGTCDTFEERQIGGDRFNLFEGCPKAKTCTLILRGGADQFIAEVERSLHDAIMI 400
Query: 187 VRRAMKNDAVVAGGGAIDMEISKYLRDYSRNIHGRDQQFIGAMARAFEVIPRQLCENAGF 366
V+ A+KN+ VVAGGGA +ME+SKYLRDYS I G+ Q FI A AR+ EVIPRQLC+NAGF
Sbjct: 401 VKHALKNNLVVAGGGACEMELSKYLRDYSLTISGKQQNFIAAFARSLEVIPRQLCDNAGF 460
Query: 367 DATNILNKLRQKHAQGKIWCGVDIMNEDIADNFEACVWEPALVKSNAITAATEAACMVLT 546
D+TNILNKLR +HA+G++W GVD+ +E +A+NFE VWEP+ VKSNAI +ATEAA ++L+
Sbjct: 461 DSTNILNKLRMQHAKGEMWAGVDMDSEGVANNFEKFVWEPSTVKSNAILSATEAATLILS 520
Query: 547 LDETIRNAKSKTPE----------DNRPVKMPGRGRP 627
+DETI+N S+ P+ NR ++ GRG P
Sbjct: 521 VDETIKNEPSQQPQAPQGALPPGAANRIMRGRGRGMP 557
>sp|P42943|TCPH_YEAST T-complex protein 1, eta subunit (TCP-1-eta) (CCT-eta)
Length = 550
Score = 247 bits (631), Expect = 2e-65
Identities = 123/207 (59%), Positives = 154/207 (74%), Gaps = 1/207 (0%)
Frame = +1
Query: 7 DITDDTLGKCESFEEVQIGGERFNIFVGCPEAKTCTIILRGGSEQFMEETERSLHDAIMI 186
DI + LG C FEE+QIG ER+N+F GCP+AKTCT++LRGG+EQ + E ERSLHDAIMI
Sbjct: 340 DIKPEHLGTCALFEEMQIGSERYNLFQGCPQAKTCTLLLRGGAEQVIAEVERSLHDAIMI 399
Query: 187 VRRAMKNDAVVAGGGAIDMEISKYLRDYSRNIHGRDQQFIGAMARAFEVIPRQLCENAGF 366
V+RA++N +VAGGGA +ME+SK LRDYS+ I G+ Q I A A+A EVIPRQLCENAGF
Sbjct: 400 VKRALQNKLIVAGGGATEMEVSKCLRDYSKTIAGKQQMIINAFAKALEVIPRQLCENAGF 459
Query: 367 DATNILNKLRQKHAQGKIWCGVDIMNEDIADNFEACVWEPALVKSNAITAATEAACMVLT 546
DA ILNKLR H++G+ W GV E+I DNF VWEPALVK NA+ +ATEA ++L+
Sbjct: 460 DAIEILNKLRLAHSKGEKWYGVVFETENIGDNFAKFVWEPALVKINALNSATEATNLILS 519
Query: 547 LDETIRNAKSKTPEDN-RPVKMPGRGR 624
+DETI N S++ P + GRGR
Sbjct: 520 VDETITNKGSESANAGMMPPQGAGRGR 546
>sp|P54409|TCPH_TETPY T-complex protein 1, eta subunit (TCP-1-eta) (CCT-eta)
Length = 558
Score = 216 bits (550), Expect = 5e-56
Identities = 113/201 (56%), Positives = 146/201 (72%), Gaps = 5/201 (2%)
Frame = +1
Query: 1 VKDITDDTLGKCESFEEVQIGGERFNIFVGCPEAKTCTIILRGGSEQFMEETERSLHDAI 180
V ++ D LG C FEE QIG ER+N+F CP +K+ TIILRGG+EQF+ E ERSL+DAI
Sbjct: 334 VNGLSQDVLGTCGMFEEQQIGAERYNLFQDCPHSKSATIILRGGAEQFIAEAERSLNDAI 393
Query: 181 MIVRRAMKNDAVVAGGGAIDMEISKYLRDYSRNIHGRDQQFIGAMARAFEVIPRQLCENA 360
MIVRR MK + +V GGGAI++EIS+ LR +SR G+ Q I A A+A EVIP+ + +NA
Sbjct: 394 MIVRRCMKANKIVPGGGAIELEISRLLRLHSRKTEGKVQLVINAFAKALEVIPKTIADNA 453
Query: 361 GFDATNILNKLRQKHA----QGKIWCGVDIMNED-IADNFEACVWEPALVKSNAITAATE 525
G D+ +LNKLRQKHA Q K + GVDI D I +NFE VWEP +V+ NA +AATE
Sbjct: 454 GHDSIQVLNKLRQKHALESDQSKNF-GVDINAVDGIGNNFENFVWEPIIVRKNAFSAATE 512
Query: 526 AACMVLTLDETIRNAKSKTPE 588
AAC +L++DET+RN KS+ P+
Sbjct: 513 AACTILSIDETVRNPKSEQPK 533
>sp|O77323|TCPH_PLAF7 T-complex protein 1, eta subunit (TCP-1-eta) (CCT-eta)
Length = 539
Score = 209 bits (533), Expect = 4e-54
Identities = 103/200 (51%), Positives = 143/200 (71%), Gaps = 2/200 (1%)
Frame = +1
Query: 7 DITDDTLGKCESFEEVQIGGERFNIFVGCPEAKTCTIILRGGSEQFMEETERSLHDAIMI 186
++ DD LG C FEEVQIG ER+NIF C + K+ TIILRGG++QF+EE ERS++DAIMI
Sbjct: 338 NLNDDVLGTCGVFEEVQIGNERYNIFKECLKTKSVTIILRGGAKQFIEEVERSINDAIMI 397
Query: 187 VRRAMKNDAVVAGGGAIDMEISKYLRDYSRNIHGRDQQFIGAMARAFEVIPRQLCENAGF 366
V R + N +V G G+I+M++SKYLR YSR+I ++Q + + A+A E IPR L NAG+
Sbjct: 398 VLRCITNSEIVPGAGSIEMQLSKYLRIYSRSICNKEQIVLFSFAKALESIPRHLSHNAGY 457
Query: 367 DATNILNKLRQKHAQ--GKIWCGVDIMNEDIADNFEACVWEPALVKSNAITAATEAACMV 540
D+T+ILNKLR+KH++ IW GVD M DI + ++ C++E +K N I +ATEAAC++
Sbjct: 458 DSTDILNKLRKKHSEQTSDIWYGVDCMEGDIINAYDNCIFEVTKIKRNVIYSATEAACLI 517
Query: 541 LTLDETIRNAKSKTPEDNRP 600
L++DETI+N S P
Sbjct: 518 LSIDETIKNPSSAAGTQRSP 537
>sp|P50016|THS_METKA Thermosome subunit (Chaperonin-like complex) (CLIC)
Length = 545
Score = 162 bits (411), Expect = 6e-40
Identities = 76/187 (40%), Positives = 125/187 (66%)
Frame = +1
Query: 1 VKDITDDTLGKCESFEEVQIGGERFNIFVGCPEAKTCTIILRGGSEQFMEETERSLHDAI 180
+ D++++ LG+ E EE ++ G++ GC + K TI++RGG+E ++E ER++ DAI
Sbjct: 338 IDDLSEEDLGEAEVVEEKKVAGDKMIFVEGCKDPKAVTILIRGGTEHVVDEAERAIEDAI 397
Query: 181 MIVRRAMKNDAVVAGGGAIDMEISKYLRDYSRNIHGRDQQFIGAMARAFEVIPRQLCENA 360
+V A+++ VVAGGGA ++E+++ LRD++ + GR+Q + A A A E+IPR L EN+
Sbjct: 398 GVVAAALEDGKVVAGGGAPEVEVARQLRDFADGVEGREQLAVEAFADALEIIPRTLAENS 457
Query: 361 GFDATNILNKLRQKHAQGKIWCGVDIMNEDIADNFEACVWEPALVKSNAITAATEAACMV 540
G D ++L +LR KH G++ G+D+ + D+ D E V EP VK+ A+ +ATEAA M+
Sbjct: 458 GLDPIDVLVQLRAKHEDGQVTAGIDVYDGDVKDMLEEGVVEPLRVKTQALASATEAAEMI 517
Query: 541 LTLDETI 561
L +D+ I
Sbjct: 518 LRIDDVI 524
>sp|O28045|THSA_ARCFU Thermosome alpha subunit (Thermosome subunit 1) (Chaperonin alpha
subunit)
Length = 545
Score = 160 bits (405), Expect = 3e-39
Identities = 87/199 (43%), Positives = 126/199 (63%), Gaps = 3/199 (1%)
Frame = +1
Query: 1 VKDITDDTLGKCESFEEVQIGGERFNIFVGCPEAKTCTIILRGGSEQFMEETERSLHDAI 180
+++IT LG+ E EE ++G E+ GC K TI++RGGSE ++E ERSL DAI
Sbjct: 336 LREITSADLGEAELVEERKVGDEKMVFIEGCKNPKAVTILIRGGSEHVVDEVERSLQDAI 395
Query: 181 MIVRRAMKNDAVVAGGGAIDMEISKYLRDYSRNIHGRDQQFIGAMARAFEVIPRQLCENA 360
+V+ A+++ VVAGGGA ++E++ +RD++ + GR+Q A A A EVIPR L EN+
Sbjct: 396 KVVKTALESGKVVAGGGAPEIEVALKIRDWAPTLGGREQLAAEAFASALEVIPRALAENS 455
Query: 361 GFDATNILNKLRQKHAQGKIWCGVDIMNEDIADNFEACVWEPALVKSNAITAATEAACMV 540
G D +IL +LR+ H +GK GVD+ + ++A E V EP VK+ AIT+ATE A M+
Sbjct: 456 GLDPIDILVELRKAHEEGKTTYGVDVFSGEVACMKERGVLEPLKVKTQAITSATEVAIMI 515
Query: 541 LTLDETIRN---AKSKTPE 588
L +D+ I K K PE
Sbjct: 516 LRIDDVIAAKGLEKEKGPE 534
>sp|P48424|THSA_THEAC Thermosome alpha subunit (Thermosome subunit 1) (Chaperonin alpha
subunit)
Length = 545
Score = 155 bits (393), Expect = 7e-38
Identities = 81/209 (38%), Positives = 126/209 (60%), Gaps = 2/209 (0%)
Frame = +1
Query: 7 DITDDTLGKCESFEEVQIGGERFNIFVGCPEAKTCTIILRGGSEQFMEETERSLHDAIMI 186
D+T LG+ E+ EE +IG +R +GC K +I++RGG++ + E ER+L+DAI +
Sbjct: 335 DLTPSVLGEAETVEERKIGDDRMTFVMGCKNPKAVSILIRGGTDHVVSEVERALNDAIRV 394
Query: 187 VRRAMKNDAVVAGGGAIDMEISKYLRDYSRNIHGRDQQFIGAMARAFEVIPRQLCENAGF 366
V ++ + GGGA++ E++ L Y+ ++ GR+Q I A A+A E+IPR L ENAG
Sbjct: 395 VAITKEDGKFLWGGGAVEAELAMRLAKYANSVGGREQLAIEAFAKALEIIPRTLAENAGI 454
Query: 367 DATNILNKLRQKHAQGKIWCGVDIMNEDIADNFEACVWEPALVKSNAITAATEAACMVLT 546
D N L KL+ +H +G+I GVD+ N + D V +P VK++A+ +A E A M+L
Sbjct: 455 DPINTLIKLKAEHEKGRISVGVDLDNNGVGDMKAKGVVDPLRVKTHALESAVEVATMILR 514
Query: 547 LDETIRNAKSKTP--EDNRPVKMPGRGRP 627
+D+ I + KS P + + MPG G P
Sbjct: 515 IDDVIASKKSTPPSGQGGQGQGMPGGGMP 543
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 78,370,165
Number of Sequences: 369166
Number of extensions: 1542751
Number of successful extensions: 4966
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4479
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4643
length of database: 68,354,980
effective HSP length: 107
effective length of database: 48,588,335
effective search space used: 5927776870
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)