Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_005_N06
(703 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P46467|VPS4B_MOUSE Vacuolar sorting protein 4b (SKD1 pro... 127 2e-29
sp|O75351|VPS4B_HUMAN Vacuolar sorting protein 4b (SKD1 pro... 127 4e-29
sp|Q8VEJ9|VPS4A_MOUSE Vacuolar sorting protein 4a 119 6e-27
sp|Q9UN37|VPS4A_HUMAN Vacuolar sorting protein 4a (SKD2 pro... 117 3e-26
sp|P52917|VPS4_YEAST Vacuolar protein sorting-associated pr... 85 2e-16
sp|Q09803|VPS4_SCHPO Suppressor protein of bem1/bed5 double... 79 9e-15
sp|Q26366|VG_DROME Vestigial protein 36 0.086
sp|Q9PUB8|KE4_BRARE Zinc transporter Slc39a7 (Solute carrie... 34 0.43
sp|P26410|HYPB_RHOCA Hydrogenase nickel incorporation prote... 33 0.56
sp|Q8N441|FGRL1_HUMAN Fibroblast growth factor receptor-lik... 33 0.56
>sp|P46467|VPS4B_MOUSE Vacuolar sorting protein 4b (SKD1 protein)
Length = 444
Score = 127 bits (320), Expect = 2e-29
Identities = 61/91 (67%), Positives = 72/91 (79%)
Frame = +1
Query: 25 PVRKVQTATHFRRVRGPSPTDPSVIVSDLLTPCSPGSPGAIEMNWQTVDSEKLLEPCVTF 204
PVRKVQ+ATHF++VRGPS DP+ IV+DLLTPCSPG PGAIEM W V +KLLEP V+
Sbjct: 352 PVRKVQSATHFKKVRGPSRADPNCIVNDLLTPCSPGDPGAIEMTWMDVPGDKLLEPVVSM 411
Query: 205 ADMKLALSRNKPTVNQKDLEKLIQFTTDFGQ 297
DM +LS KPTVN++DL KL +FT DFGQ
Sbjct: 412 WDMLRSLSSTKPTVNEQDLLKLKKFTEDFGQ 442
>sp|O75351|VPS4B_HUMAN Vacuolar sorting protein 4b (SKD1 protein)
Length = 444
Score = 127 bits (318), Expect = 4e-29
Identities = 60/91 (65%), Positives = 71/91 (78%)
Frame = +1
Query: 25 PVRKVQTATHFRRVRGPSPTDPSVIVSDLLTPCSPGSPGAIEMNWQTVDSEKLLEPCVTF 204
PVRKVQ+ATHF++VRGPS DP+ +V DLLTPCSPG PGAIEM W V +KLLEP V+
Sbjct: 352 PVRKVQSATHFKKVRGPSRADPNHLVDDLLTPCSPGDPGAIEMTWMDVPGDKLLEPVVSM 411
Query: 205 ADMKLALSRNKPTVNQKDLEKLIQFTTDFGQ 297
+DM +LS KPTVN+ DL KL +FT DFGQ
Sbjct: 412 SDMLRSLSNTKPTVNEHDLLKLKKFTEDFGQ 442
>sp|Q8VEJ9|VPS4A_MOUSE Vacuolar sorting protein 4a
Length = 437
Score = 119 bits (299), Expect = 6e-27
Identities = 56/91 (61%), Positives = 70/91 (76%)
Frame = +1
Query: 25 PVRKVQTATHFRRVRGPSPTDPSVIVSDLLTPCSPGSPGAIEMNWQTVDSEKLLEPCVTF 204
PVRKVQ+ATHF++V GPS T+PSV++ DLLTPCSPG PGAIEM W V +KLLEP V
Sbjct: 345 PVRKVQSATHFKKVCGPSRTNPSVMIDDLLTPCSPGDPGAIEMTWMDVPGDKLLEPVVCM 404
Query: 205 ADMKLALSRNKPTVNQKDLEKLIQFTTDFGQ 297
+DM +L+ +PTVN DL K+ +F+ DFGQ
Sbjct: 405 SDMLRSLATTRPTVNADDLLKVKKFSEDFGQ 435
>sp|Q9UN37|VPS4A_HUMAN Vacuolar sorting protein 4a (SKD2 protein) (hVPS4) (VPS4-1)
Length = 437
Score = 117 bits (293), Expect = 3e-26
Identities = 54/91 (59%), Positives = 70/91 (76%)
Frame = +1
Query: 25 PVRKVQTATHFRRVRGPSPTDPSVIVSDLLTPCSPGSPGAIEMNWQTVDSEKLLEPCVTF 204
PVRKVQ+ATHF++V GPS T+PS+++ DLLTPCSPG PGA+EM W V +KLLEP V
Sbjct: 345 PVRKVQSATHFKKVCGPSRTNPSMMIDDLLTPCSPGDPGAMEMTWMDVPGDKLLEPVVCM 404
Query: 205 ADMKLALSRNKPTVNQKDLEKLIQFTTDFGQ 297
+DM +L+ +PTVN DL K+ +F+ DFGQ
Sbjct: 405 SDMLRSLATTRPTVNADDLLKVKKFSEDFGQ 435
>sp|P52917|VPS4_YEAST Vacuolar protein sorting-associated protein VPS4 (END13 protein)
(DOA4-independent degradation protein 6)
Length = 437
Score = 85.1 bits (209), Expect = 2e-16
Identities = 44/91 (48%), Positives = 59/91 (64%)
Frame = +1
Query: 25 PVRKVQTATHFRRVRGPSPTDPSVIVSDLLTPCSPGSPGAIEMNWQTVDSEKLLEPCVTF 204
P+RK+Q+ATHF+ V S D + LTPCSPG GAIEM+W +++++L EP +T
Sbjct: 350 PIRKIQSATHFKDV---STEDDE---TRKLTPCSPGDDGAIEMSWTDIEADELKEPDLTI 403
Query: 205 ADMKLALSRNKPTVNQKDLEKLIQFTTDFGQ 297
D A+ +PTVN+ DL K QFT DFGQ
Sbjct: 404 KDFLKAIKSTRPTVNEDDLLKQEQFTRDFGQ 434
>sp|Q09803|VPS4_SCHPO Suppressor protein of bem1/bed5 double mutants
Length = 432
Score = 79.3 bits (194), Expect = 9e-15
Identities = 40/90 (44%), Positives = 53/90 (58%)
Frame = +1
Query: 25 PVRKVQTATHFRRVRGPSPTDPSVIVSDLLTPCSPGSPGAIEMNWQTVDSEKLLEPCVTF 204
PVR++ TATHF+ V L+TPCSPG P A E +W V+ E ++EP +T
Sbjct: 346 PVRRIHTATHFKEVYDNKSN------RTLVTPCSPGDPDAFESSWLEVNPEDIMEPKLTV 399
Query: 205 ADMKLALSRNKPTVNQKDLEKLIQFTTDFG 294
D A+ + KPT+N D+EK QFT DFG
Sbjct: 400 RDFYSAVRKVKPTLNAGDIEKHTQFTKDFG 429
>sp|Q26366|VG_DROME Vestigial protein
Length = 453
Score = 36.2 bits (82), Expect = 0.086
Identities = 15/28 (53%), Positives = 18/28 (64%)
Frame = -2
Query: 600 LTASHAHTHAHNLVHSEPQAHQHAHTHT 517
L +SH THAH+L H+ H H HTHT
Sbjct: 150 LHSSH-RTHAHSLAHAHTHPHSHTHTHT 176
>sp|Q9PUB8|KE4_BRARE Zinc transporter Slc39a7 (Solute carrier family 39 member 7)
(Histidine-rich membrane protein Ke4)
Length = 352
Score = 33.9 bits (76), Expect = 0.43
Identities = 11/31 (35%), Positives = 15/31 (48%)
Frame = -2
Query: 612 HLYILTASHAHTHAHNLVHSEPQAHQHAHTH 520
H + H H H+H +H AH+H H H
Sbjct: 73 HTHDHAHDHGHAHSHGDIHDHGHAHKHGHAH 103
Score = 30.8 bits (68), Expect = 3.6
Identities = 11/31 (35%), Positives = 14/31 (45%)
Frame = -2
Query: 612 HLYILTASHAHTHAHNLVHSEPQAHQHAHTH 520
H++ +H H H H HS H H H H
Sbjct: 67 HVHDHGHTHDHAHDHGHAHSHGDIHDHGHAH 97
>sp|P26410|HYPB_RHOCA Hydrogenase nickel incorporation protein hypB
Length = 335
Score = 33.5 bits (75), Expect = 0.56
Identities = 12/22 (54%), Positives = 14/22 (63%)
Frame = -2
Query: 591 SHAHTHAHNLVHSEPQAHQHAH 526
SH HTHA H+ P AH H+H
Sbjct: 49 SHWHTHADGTSHAHPHAHSHSH 70
Score = 31.6 bits (70), Expect = 2.1
Identities = 11/25 (44%), Positives = 15/25 (60%)
Frame = -2
Query: 591 SHAHTHAHNLVHSEPQAHQHAHTHT 517
SH+H+H H AH HAH+H+
Sbjct: 45 SHSHSHWHTHADGTSHAHPHAHSHS 69
Score = 30.8 bits (68), Expect = 3.6
Identities = 9/24 (37%), Positives = 16/24 (66%)
Frame = -2
Query: 588 HAHTHAHNLVHSEPQAHQHAHTHT 517
H+H+H+H H++ +H H H H+
Sbjct: 44 HSHSHSHWHTHADGTSHAHPHAHS 67
Score = 30.4 bits (67), Expect = 4.7
Identities = 15/42 (35%), Positives = 19/42 (45%), Gaps = 10/42 (23%)
Frame = -2
Query: 612 HLYILTASHAHTHAHNLVHS----------EPQAHQHAHTHT 517
H + SHAH HAH+ H+ P AH H H H+
Sbjct: 52 HTHADGTSHAHPHAHSHSHAAGHGAEADSDHPHAHGHDHGHS 93
>sp|Q8N441|FGRL1_HUMAN Fibroblast growth factor receptor-like 1 precursor (FGF
receptor-like protein 1) (Fibroblast growth factor
receptor 5) (FGFR-like protein) (FGF homologous factor
receptor)
Length = 504
Score = 33.5 bits (75), Expect = 0.56
Identities = 13/28 (46%), Positives = 16/28 (57%)
Frame = -2
Query: 609 LYILTASHAHTHAHNLVHSEPQAHQHAH 526
LY +H HTH+H H E + HQH H
Sbjct: 474 LYTDIHTHTHTHSHTHSHVEGKVHQHIH 501
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 77,938,324
Number of Sequences: 369166
Number of extensions: 1553375
Number of successful extensions: 5897
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4910
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5653
length of database: 68,354,980
effective HSP length: 107
effective length of database: 48,588,335
effective search space used: 6122130210
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)