Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_005_M21
(903 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P87048|RPN1_SCHPO 26S proteasome regulatory subunit rpn1... 38 0.044
sp|Q6CJL0|SNF7_KLULA Vacuolar sorting protein SNF7 (Vacuola... 36 0.13
sp|P34618|CEC1_CAEEL Chromo domain protein cec-1 36 0.13
sp|Q12114|CHS5_YEAST Chitin biosynthesis protein CHS5 (Prot... 36 0.13
sp|P36100|T2EA_YEAST Transcription initiation factor IIE al... 35 0.38
sp|Q8D8M2|FTSK_VIBVU DNA translocase ftsK 35 0.38
sp|Q01042|IE68_SHV21 Immediate-early protein 34 0.64
sp|Q9SCY0|PGMP_ARATH Phosphoglucomutase, chloroplast precur... 33 0.84
sp|P20281|RS3_HALMA 30S ribosomal protein S3P (HmaS3) (HS1) 33 0.84
sp|P23466|CYAA_SACKL Adenylate cyclase (ATP pyrophosphate-l... 33 1.1
>sp|P87048|RPN1_SCHPO 26S proteasome regulatory subunit rpn1 (Proteasome non-ATPase
subunit mts4) (19S regulatory cap region of 26S protease
subunit 2)
Length = 891
Score = 37.7 bits (86), Expect = 0.044
Identities = 22/89 (24%), Positives = 48/89 (53%), Gaps = 13/89 (14%)
Frame = +2
Query: 176 DLSTQHISSNGNVT----TEAHESDDQSISNEIEIENGVEDVKEEE---------LIKMV 316
D+S++ S N + T+ E++D++ +N + + +ED+ EE+ L++ V
Sbjct: 5 DISSKSPSGNDALNDKKGTKTSETNDRNSTNNTKERDELEDLSEEDLQLKNDLELLVQAV 64
Query: 317 SDAVDEIMGDAKSQVQHIVETPVSKDNSI 403
DA E++G + +Q++ I+ T S ++
Sbjct: 65 QDATPELVGSSLTQLKEIIRTSTSSMTAV 93
>sp|Q6CJL0|SNF7_KLULA Vacuolar sorting protein SNF7 (Vacuolar protein sorting-associated
protein VPS32)
Length = 232
Score = 36.2 bits (82), Expect = 0.13
Identities = 26/96 (27%), Positives = 41/96 (42%), Gaps = 5/96 (5%)
Frame = +2
Query: 8 NVEKESSDHK*NISSDVMLEDEFKPVEDLAEETKTSAEHTEPIQNSEITENSI-----VD 172
N E + K I D + EDE +L E EH P+ +I + SI D
Sbjct: 137 NQEISEAISKPLILQDELDEDELDQELELLESENLQKEHIAPVPQQQIQQPSIDLPSVPD 196
Query: 173 IDLSTQHISSNGNVTTEAHESDDQSISNEIEIENGV 280
+ LST ++S + E +D+ E++ E G+
Sbjct: 197 VKLSTPELNSAQADEEDEEEDEDERALRELQAEMGL 232
>sp|P34618|CEC1_CAEEL Chromo domain protein cec-1
Length = 304
Score = 36.2 bits (82), Expect = 0.13
Identities = 24/110 (21%), Positives = 50/110 (45%), Gaps = 5/110 (4%)
Frame = +2
Query: 2 DENVEKESSDHK*NISSDVMLEDEFKPVEDLAEETKTSAEHTEPIQNSEITENSIV---- 169
D + E S+D K + +ED+ +PV +E + E E ++ + E+ V
Sbjct: 127 DTDEEHSSADKKSKAEDEEEVEDDEEPVPKKKKEVQEEPEEEESVEGEDEEESQEVEDLK 186
Query: 170 -DIDLSTQHISSNGNVTTEAHESDDQSISNEIEIENGVEDVKEEELIKMV 316
D + +V E+ +++ + ++E + E+ +EEE I++V
Sbjct: 187 EDEKMEEDEKEEEEDVQLESEKNEKEEEEEKVEEKKEEEEEEEEEEIQLV 236
>sp|Q12114|CHS5_YEAST Chitin biosynthesis protein CHS5 (Protein CAL3)
Length = 671
Score = 36.2 bits (82), Expect = 0.13
Identities = 29/123 (23%), Positives = 51/123 (41%), Gaps = 5/123 (4%)
Frame = +2
Query: 62 LEDEFKPVEDLAEETKTSAEHTEPIQNSEITENSIVDIDLSTQHISSNGNVTTEAHESDD 241
+ED +PVED E + + E T I + E DI+L + + +V E D
Sbjct: 527 VEDTSEPVEDAGEPVQETNEFTTDIASPRHQEE---DIELEAEPKDATESVAVEPSNEDV 583
Query: 242 QSISNEIEIENGVEDVKE-----EELIKMVSDAVDEIMGDAKSQVQHIVETPVSKDNSID 406
+ E E+ + +V + E ++A + A ++ Q E V+ D+ +
Sbjct: 584 KPEEKGSEAEDDINNVSKEAASGESTTHQKTEASASLESSAVTEEQETTEAEVNTDDVLS 643
Query: 407 TLE 415
T E
Sbjct: 644 TKE 646
>sp|P36100|T2EA_YEAST Transcription initiation factor IIE alpha subunit (TFIIE-alpha)
(Transcription factor A large subunit) (Factor A 66 kDa
subunit)
Length = 482
Score = 34.7 bits (78), Expect = 0.38
Identities = 25/101 (24%), Positives = 43/101 (42%), Gaps = 5/101 (4%)
Frame = +2
Query: 2 DENVEKESS-----DHK*NISSDVMLEDEFKPVEDLAEETKTSAEHTEPIQNSEITENSI 166
DE E+E D + + LEDEF+ V D A KT + + ++ I + +
Sbjct: 384 DEEEEEEEEMEDVMDDNDETARENALEDEFEDVTDTAGTAKTESNTSNDVKQESINDKTE 443
Query: 167 VDIDLSTQHISSNGNVTTEAHESDDQSISNEIEIENGVEDV 289
++ + + N + DD +E++IE EDV
Sbjct: 444 DAVNATATASGPSANAKPNDGDDDDDDDDDEMDIE--FEDV 482
>sp|Q8D8M2|FTSK_VIBVU DNA translocase ftsK
Length = 990
Score = 34.7 bits (78), Expect = 0.38
Identities = 31/143 (21%), Positives = 61/143 (42%), Gaps = 1/143 (0%)
Frame = +2
Query: 5 ENVEKESSDHK*NISSDVMLEDEFKPVEDLAEETKTSAEHTEPIQNSEITENSIVDIDLS 184
E+ + +H + S DV +EF V L+E K S E EP+ + +
Sbjct: 341 EHSQTTQVEHAQDFSVDV---EEFDHVISLSELDKISEEIDEPVM-----------VGFA 386
Query: 185 TQHISSNGNVTTEAHESDDQSISNEIEIENGVEDVKEEELIKM-VSDAVDEIMGDAKSQV 361
+ + A S + + + + +E + +EE++ + V+D+V GD ++
Sbjct: 387 EEAPLHHNEAQRSAMASSAEPMFSHLGVEQTTQHTTQEEIVDLPVADSV----GDVNPEM 442
Query: 362 QHIVETPVSKDNSIDTLEN*ISK 430
+ VE +D + +N +SK
Sbjct: 443 EDYVEEDEDQDQDVVAFQNMVSK 465
>sp|Q01042|IE68_SHV21 Immediate-early protein
Length = 407
Score = 33.9 bits (76), Expect = 0.64
Identities = 24/111 (21%), Positives = 48/111 (43%)
Frame = +2
Query: 2 DENVEKESSDHK*NISSDVMLEDEFKPVEDLAEETKTSAEHTEPIQNSEITENSIVDIDL 181
+E E+E ++ + ++ E+E + E+ AEE + E E + +E E +
Sbjct: 130 EEEAEEEEAEEE---EAEEAEEEEAEEAEEEAEEEEAEEEAEEEAEEAEEAEEEAEEEAE 186
Query: 182 STQHISSNGNVTTEAHESDDQSISNEIEIENGVEDVKEEELIKMVSDAVDE 334
+ EA E+++++ E E E E + EE + +A +E
Sbjct: 187 EAEEAEEAEEAEEEAEEAEEEAEEAEEEAEEAEEAEEAEEAEEEAEEAEEE 237
Score = 32.7 bits (73), Expect = 1.4
Identities = 25/111 (22%), Positives = 46/111 (41%)
Frame = +2
Query: 2 DENVEKESSDHK*NISSDVMLEDEFKPVEDLAEETKTSAEHTEPIQNSEITENSIVDIDL 181
+E EKE+ + + + + E+E + E AEE + E E + E E + +
Sbjct: 99 EEAEEKEAEEEEAEEAEEEAEEEEAEEAE--AEEEEAEEEEAEEEEAEEAEEEEAEEAEE 156
Query: 182 STQHISSNGNVTTEAHESDDQSISNEIEIENGVEDVKEEELIKMVSDAVDE 334
+ + EA E+++ E E E E + EE + +A +E
Sbjct: 157 EAEEEEAEEEAEEEAEEAEEAEEEAEEEAEEAEEAEEAEEAEEEAEEAEEE 207
>sp|Q9SCY0|PGMP_ARATH Phosphoglucomutase, chloroplast precursor (Glucose phosphomutase)
(PGM)
Length = 623
Score = 33.5 bits (75), Expect = 0.84
Identities = 31/102 (30%), Positives = 49/102 (48%), Gaps = 1/102 (0%)
Frame = +2
Query: 95 AEETKTSAEHTEPIQNSEITENSIVDIDLSTQHISSNGNVTTEAHESDDQSISNEIEIEN 274
A ET T + + SEI I DIDLS ++ GN + E + +S+ +E+
Sbjct: 203 APETITDKIYGNTLSISEIKVAEIPDIDLSQVGVTKYGNFSVEVID----PVSDYLEL-- 256
Query: 275 GVEDVKEEELIK-MVSDAVDEIMGDAKSQVQHIVETPVSKDN 397
+EDV + +LI+ ++S + M DA V P+ DN
Sbjct: 257 -MEDVFDFDLIRGLLSRSDFGFMFDAMHAVTGAYAKPIFVDN 297
>sp|P20281|RS3_HALMA 30S ribosomal protein S3P (HmaS3) (HS1)
Length = 304
Score = 33.5 bits (75), Expect = 0.84
Identities = 29/118 (24%), Positives = 61/118 (51%), Gaps = 7/118 (5%)
Frame = +2
Query: 44 ISSDVMLEDEFKPVEDLAEE---TKTSAEHTEPIQNSEITENSIVDIDLSTQHISSNGNV 214
I + L D+F+ ED+ E T E E + E E+S +L + +++ +
Sbjct: 183 IPPNAELPDDFEIYEDVDVEDYVADTDGESVEELLEGE-PEDSETAEELD-EDVAAGADD 240
Query: 215 TTEAHES--DDQSISNEIEI--ENGVEDVKEEELIKMVSDAVDEIMGDAKSQVQHIVE 376
+EA E D++ + ++E+ ++ VEDV +EL + V + +DE D +++ + +++
Sbjct: 241 DSEADEEFVDEEIVEEDVEVPTDDDVEDVDVDELEEAVDEELDE---DVEAEAEELMD 295
>sp|P23466|CYAA_SACKL Adenylate cyclase (ATP pyrophosphate-lyase) (Adenylyl cyclase)
Length = 1839
Score = 33.1 bits (74), Expect = 1.1
Identities = 24/96 (25%), Positives = 45/96 (46%), Gaps = 1/96 (1%)
Frame = +2
Query: 17 KESSDHK*NISSDVMLEDEFKPVEDLAEETKTSAEHTEPIQNSE-ITENSIVDIDLSTQH 193
K S+ I ++E E +P E + T + + ++ ++ I E+S + STQ
Sbjct: 353 KHYSNFSKPIEDKAVVEKEQEPPEKCPQPISTDSNDAQGLEIAKPIDESSGIQHSASTQS 412
Query: 194 ISSNGNVTTEAHESDDQSISNEIEIENGVEDVKEEE 301
+SS + T A + + I + E E+ + V E+E
Sbjct: 413 VSSFSSGATGASGATGKQIGGDQETESTISTVGEDE 448
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 77,330,583
Number of Sequences: 369166
Number of extensions: 1295638
Number of successful extensions: 4188
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3976
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4170
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 9126484700
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)