Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_005_M17
(969 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P43883|ADFP_MOUSE Adipophilin (Adipose differentiation-r... 40 0.008
sp|P55737|HSP82_ARATH Heat shock protein 81-2 (HSP81-2) 33 1.6
sp|P51818|HSP83_ARATH Heat shock protein 81-3 (HSP81-3) (HS... 33 1.6
sp|Q99541|ADFP_HUMAN Adipophilin (Adipose differentiation-r... 32 2.7
sp|Q8AYI2|PRGR_RANDY Progesterone receptor (PR) (dyPR) 32 3.5
sp|P57498|CYSN_BUCAI Sulfate adenylyltransferase subunit 1 ... 32 3.5
sp|P03589|V125_AMVLE 125 kDa protein 31 4.6
sp|Q06412|TUS1_YEAST Rho1 guanine nucleotide exchange facto... 31 4.6
sp|P80872|G16O_BACSU General stress protein 16O (GSP16O) 31 4.6
sp|P33126|HSP82_ORYSA Heat shock protein 82 31 6.0
>sp|P43883|ADFP_MOUSE Adipophilin (Adipose differentiation-related protein) (ADRP)
Length = 425
Score = 40.4 bits (93), Expect = 0.008
Identities = 60/307 (19%), Positives = 120/307 (39%), Gaps = 19/307 (6%)
Frame = +3
Query: 3 TFTKAGEYYTWIKEKNSKLNSTLTWTESNLKIVKDMVEPIAAS-------KLGSVDNFAC 161
T+ Y K++ L S E +K V A ++ + +AC
Sbjct: 25 TYDLVSSAYVSTKDQYPYLRSVCEMAEKGVKTVTSAAMTSALPIIQKLEPQIAVANTYAC 84
Query: 162 NQLDKLETKYPXXXXXXXXXXXXXGEYYDSSIKPNVDKMIAAKDSAVYIAKTPFRITGQT 341
LD++E + P + +++A+ AV AK + T
Sbjct: 85 KGLDRMEERLPI-------------------LNQPTSEIVASARGAVTGAKD---VVTTT 122
Query: 342 IEFLSNTTNSFVHRFMSKDKKAEYGAVEKKEYDILYRRALKEVNTILTFTLKNIEGLSNV 521
+ ++ S V + K K A G+VE+ + +++ ++ + G+
Sbjct: 123 MAGAKDSVASTVSGVVDKTKGAVTGSVERTK-------------SVVNGSINTVLGMVQF 169
Query: 522 LNSIYDQSVTKNKEWLHGYY----EEFTDEMKKNSGDESKTESTVMQLAQNFSNKL--RV 683
+NS D ++TK++ + Y+ EE E KK G + + + + ++ S KL R
Sbjct: 170 MNSGVDNAITKSEMLVDQYFPLTQEELEMEAKKVEGFDMVQKPSNYERLESLSTKLCSRA 229
Query: 684 SVRAMSTVCYNHMSSGNKETLDLMFNKTEHIYSTLSKSTY------QEIRDQISIENIKW 845
+A+S V ++ET+ + T H+ K+ + Q +D++ + ++W
Sbjct: 230 YHQALSRV--KEAKQKSQETIS-QLHSTVHLIEFARKNMHSANQKIQGAQDKLYVSWVEW 286
Query: 846 IGQNSIG 866
+ SIG
Sbjct: 287 --KRSIG 291
>sp|P55737|HSP82_ARATH Heat shock protein 81-2 (HSP81-2)
Length = 699
Score = 32.7 bits (73), Expect = 1.6
Identities = 32/161 (19%), Positives = 70/161 (43%), Gaps = 3/161 (1%)
Frame = +3
Query: 414 GAVEKKEYDILYRRALKEVNTILTFTLKNIEGLSNVLNSIYDQSVTKNKEWLHGYYEEFT 593
G V+ ++ + R + N IL KN+ + L ++ + +NKE + +YE F+
Sbjct: 358 GIVDSEDLPLNISRETLQQNKILKVIRKNL--VKKCLELFFE--IAENKEDYNKFYEAFS 413
Query: 594 DEMKKNSGDESKTESTVMQLAQNFSNKLRVSVRAMSTVCYNHMSSGNKETLDLMFNKTEH 773
+K ++S+ + + +L + S K + ++ M G + +F T
Sbjct: 414 KNLKLGIHEDSQNRTKIAELLRYHSTKSGDELTSLKDY-VTRMKEGQND----IFYITGE 468
Query: 774 IYSTLSKSTYQEIRDQISIE---NIKWIGQNSIGTIEQYQG 887
+ S + E + IE + I + +IG +++++G
Sbjct: 469 SKKAVENSPFLEKLKKKGIEVLYMVDAIDEYAIGQLKEFEG 509
>sp|P51818|HSP83_ARATH Heat shock protein 81-3 (HSP81-3) (HSP81.2)
Length = 699
Score = 32.7 bits (73), Expect = 1.6
Identities = 32/161 (19%), Positives = 70/161 (43%), Gaps = 3/161 (1%)
Frame = +3
Query: 414 GAVEKKEYDILYRRALKEVNTILTFTLKNIEGLSNVLNSIYDQSVTKNKEWLHGYYEEFT 593
G V+ ++ + R + N IL KN+ + L ++ + +NKE + +YE F+
Sbjct: 358 GIVDSEDLPLNISRETLQQNKILKVIRKNL--VKKCLELFFE--IAENKEDYNKFYEAFS 413
Query: 594 DEMKKNSGDESKTESTVMQLAQNFSNKLRVSVRAMSTVCYNHMSSGNKETLDLMFNKTEH 773
+K ++S+ + + +L + S K + ++ M G + +F T
Sbjct: 414 KNLKLGIHEDSQNRTKIAELLRYHSTKSGDELTSLKDY-VTRMKEGQND----IFYITGE 468
Query: 774 IYSTLSKSTYQEIRDQISIE---NIKWIGQNSIGTIEQYQG 887
+ S + E + IE + I + +IG +++++G
Sbjct: 469 SKKAVENSPFLEKLKKKGIEVLYMVDAIDEYAIGQLKEFEG 509
>sp|Q99541|ADFP_HUMAN Adipophilin (Adipose differentiation-related protein) (ADRP)
Length = 437
Score = 32.0 bits (71), Expect = 2.7
Identities = 59/303 (19%), Positives = 117/303 (38%), Gaps = 15/303 (4%)
Frame = +3
Query: 3 TFTKAGEYYTWIKEKNSKLNSTLTWTESNLKIVKDMVEPIAAS-------KLGSVDNFAC 161
T+ Y K++ L S E+ +K + + A ++ + +AC
Sbjct: 25 TYDLMSSAYLSTKDQYPYLKSVCEMAENGVKTITSVAMTSALPIIQKLEPQIAVANTYAC 84
Query: 162 NQLDKLETKYPXXXXXXXXXXXXXGEYYDSSIKPNVDKMIAAKDSAVYIAKTPFRITGQT 341
LD++E + P + +++A AV AK T
Sbjct: 85 KGLDRIEERLPI-------------------LNQPSTQIVANAKGAVTGAKD---AVTTT 122
Query: 342 IEFLSNTTNSFVHRFMSKDKKAEYGAVEKKEYDILYRRALKEVNTILTFTLKNIEGLSNV 521
+ ++ S + M K K A G+VEK + + +NT+L + + +S+
Sbjct: 123 VTGAKDSVASTITGVMDKTKGAVTGSVEKTKSVV-----SGSINTVLGSRMMQL--VSSG 175
Query: 522 LNSIYDQSVTKNKEWLHGYYEEFTDEMKKNSGDESKTESTVMQLAQNFSNKL--RVSVRA 695
+ + +S +++L EE E KK G + + + + S KL R +A
Sbjct: 176 VENALTKSELLVEQYLPLTEEELEKEAKKVEGFDLVQKPSYYVRLGSLSTKLHSRAYQQA 235
Query: 696 MSTVCYNHMSSGNKETLDLMFNKTEHIYSTLSKSTY------QEIRDQISIENIKWIGQN 857
+S V +++T+ + T H+ K+ Y Q+ +D++ + ++W +
Sbjct: 236 LSRV--KEAKQKSQQTIS-QLHSTVHLIEFARKNVYSANQKIQDAQDKLYLSWVEW--KR 290
Query: 858 SIG 866
SIG
Sbjct: 291 SIG 293
>sp|Q8AYI2|PRGR_RANDY Progesterone receptor (PR) (dyPR)
Length = 711
Score = 31.6 bits (70), Expect = 3.5
Identities = 14/39 (35%), Positives = 24/39 (61%)
Frame = +1
Query: 688 SEPCQPCAIITCLRETRKRWILCSIKPSISTPPYQNQHI 804
++P P A++ L + +R +LC +K S S P ++N HI
Sbjct: 484 TKPETPSALLCSLNQLCERQLLCVVKWSKSLPGFRNMHI 522
>sp|P57498|CYSN_BUCAI Sulfate adenylyltransferase subunit 1 (Sulfate adenylate
transferase) (SAT) (ATP-sulfurylase large subunit)
Length = 473
Score = 31.6 bits (70), Expect = 3.5
Identities = 42/160 (26%), Positives = 73/160 (45%), Gaps = 15/160 (9%)
Frame = +3
Query: 333 GQTIEFLSNTTNSFVHRFMSKD---KKAEYG----AVEKKEYDILYRRALKEVNTILTFT 491
GQTI+ L NS V R ++ D KKAE G V K E DI +++IL +
Sbjct: 269 GQTIKILPENINSRVSRIVTFDKELKKAEIGESITVVLKDEIDINRGDFFVNIDSILQPS 328
Query: 492 LKNIEGL----SNVLNSIYDQSVTKNKEWLHGYYEEFTDEMKKNSGDESKTESTVMQLAQ 659
+ I + N+L + +V + + + Y +E ++ N+ + K+ S V+
Sbjct: 329 QEAIIDIVWMTDNILLAGESYNVKLSGKKIRVYIKEILFKLDVNTLKKVKSHSLVL---- 384
Query: 660 NFSNKLRVSVRAMSTVCYNHMS----SGNKETLDLMFNKT 767
++ RV + V +++ S +GN +DL+ N T
Sbjct: 385 --NSIGRVKIYFSKPVIFDNYSENRMTGNMIFIDLLTNIT 422
>sp|P03589|V125_AMVLE 125 kDa protein
Length = 1126
Score = 31.2 bits (69), Expect = 4.6
Identities = 28/113 (24%), Positives = 50/113 (44%), Gaps = 10/113 (8%)
Frame = +3
Query: 444 LYRRALKEVNTILTFTLKNIEGLSNVLNS-IYDQSV--TKNKEWLHG-------YYEEFT 593
LY+ K++ T+ +F L I+G S +++ +Y QS+ EW G Y
Sbjct: 556 LYQSFQKKIETVFSFILGKIDGPSPLISDPVYFQSLEDVYYAEWHQGNAIDASNYARTLL 615
Query: 594 DEMKKNSGDESKTESTVMQLAQNFSNKLRVSVRAMSTVCYNHMSSGNKETLDL 752
D+++K + K ++ ++ AQ KL ++ + H G E L L
Sbjct: 616 DDIRKQKEESLKAKAKEVEDAQ----KLNRAILQVHAYLEAHPDGGKIEGLGL 664
>sp|Q06412|TUS1_YEAST Rho1 guanine nucleotide exchange factor TUS1 (TOR unique function
suppressor protein 1)
Length = 1307
Score = 31.2 bits (69), Expect = 4.6
Identities = 30/118 (25%), Positives = 53/118 (44%), Gaps = 4/118 (3%)
Frame = +3
Query: 417 AVEKKEYDILYRRALKEVNTILTFTLKNIEGLSNV----LNSIYDQSVTKNKEWLHGYYE 584
AV K+ ILY+ + ++ +L L + + ++++ LNS+ +S T +K+ E
Sbjct: 1108 AVCTKKTVILYQDSFEDNGIVLPSFLNDKDMMAHLRHPHLNSLPFKSATDSKK--RPSIE 1165
Query: 585 EFTDEMKKNSGDESKTESTVMQLAQNFSNKLRVSVRAMSTVCYNHMSSGNKETLDLMF 758
T+E KK+ Q++Q+ L + CY MS K+ L L F
Sbjct: 1166 SLTEEAKKDIATCKAIPVNFFQISQSSFFALVYDEAVVKINCYGEMSDWRKDILLLDF 1223
>sp|P80872|G16O_BACSU General stress protein 16O (GSP16O)
Length = 163
Score = 31.2 bits (69), Expect = 4.6
Identities = 22/105 (20%), Positives = 47/105 (44%)
Frame = +3
Query: 324 RITGQTIEFLSNTTNSFVHRFMSKDKKAEYGAVEKKEYDILYRRALKEVNTILTFTLKNI 503
R+T QT++ + V+R + K K YG EK +I Y R + + +
Sbjct: 57 RMTDQTVKEIDRELLEEVNRALQKMKDGTYGVCEKTGQEIPYERL-----EAVPYARMTV 111
Query: 504 EGLSNVLNSIYDQSVTKNKEWLHGYYEEFTDEMKKNSGDESKTES 638
E ++V + + + + +E ++E+ D K + D+ +++
Sbjct: 112 EAQADVEDDLETDAPSYERE----FHEQVKDLSNKETIDQKSSQT 152
>sp|P33126|HSP82_ORYSA Heat shock protein 82
Length = 699
Score = 30.8 bits (68), Expect = 6.0
Identities = 19/87 (21%), Positives = 42/87 (48%)
Frame = +3
Query: 414 GAVEKKEYDILYRRALKEVNTILTFTLKNIEGLSNVLNSIYDQSVTKNKEWLHGYYEEFT 593
G V+ ++ + R + + N IL KN+ + + ++ + +NKE + +YE F+
Sbjct: 360 GIVDSEDLPLNISREMLQQNKILKVIRKNL--VKKCVELFFE--IAENKEDYNKFYEAFS 415
Query: 594 DEMKKNSGDESKTESTVMQLAQNFSNK 674
+K ++S + + +L + S K
Sbjct: 416 KNLKLGIHEDSTNRNKIAELLRYHSTK 442
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 106,261,888
Number of Sequences: 369166
Number of extensions: 2118360
Number of successful extensions: 6168
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5861
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6163
length of database: 68,354,980
effective HSP length: 111
effective length of database: 47,849,395
effective search space used: 10096222345
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)